Rorug06G0212100

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0010707
Physical Location & Seq
Forward (+)
33731308 .. 33734677
3370 bp
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UTR
Exon/CDS
Intron
Rorug06G0212100.1

Sequence Viewer

Length: 1350 bp
ATGGAGTTCAATATGGAGCCTCTAAGCAATGAAATGGAGATTGCTCAATCTTTGCTACACATGCAACAAACAAACATAACTAATGAGTTTCATAATGTGGTTGGGATTGTGAATGAGTTTACGGATGGCTCGAACTCTGAAGCTGAAGAGGATGATAGCTTTAATTTGCGTGGAGTAAATAGGCCAAATTGGGCAAAAGGAGGGAATGTGGAATACTTGAAGTTCAATGATGAAGGGCAAGCTGTGGAGCCAGAAGCAACTGTTGCAAGATGGCAACGATACCTTGGCATGAAGGCCACAGATCATAGGTTGTTTCAGCTCAATGTCTTTGATTGGCGAGAGTTTCGTAAAGGAAAGAAACTAGATGAGGCATGGGATGCAATCAGGAGAACTATTGATTGGTCAGACCCCGAAACTCAAAGAAAGAGTGGGAGGATAAGGTTTGTGGTGGAGAGGAAGTTGAATGATCGTTGGAAGACATTTAAGGCAAAACTCCGGAAAGTATGGTATACCCCAAATGTTGGTACTGAAGAGCGTTTTAAATGCAAAGATGGGAGGGTAAGCAAAGAACAATGGGTTGCACTAGTTAACCACTGGGAAGATGAAAAGGAACAGTGGCGTTCTATTGTGAACAAGCAGAGTCGGGCAAAGCGTAGGATGATGCACACAACTGGCACAAAGACATTTGCAGAGATCAGGAACAAATATAAAAAAATAAATGGAGGTGTAAACCCAGACCGTTGCAAAATGTTTGAGCTTACCCATGTCCACCCTGATGAACCAAATTCTAAACAAGCTATTGCCTTAATGAAGGACAAGATTGACAAGATGAAGACTACAAAGAGGGCATCCCAACTTCCAGAGACTCTTAATGATTTTGAAATCAATGAAGTATATGTGTCTGTGCTTGGAAAAGAGACACGTGGAGGGGTGCGAGGATTTGGCTTTGGAGTCAGACCTGAACAAGTTCCTGGTGTACTAGTGCAGAAGAGAGGTGTTCATTTAGAGGTACAAGCAATGAGAGAACAACATGAGGCTGAGATTGAAACGATTCGGAAGGAGAGCCAAGAAAAGGAAGATAAGCTTAGGGAAGAACTGAACAAACAAACTCTGGCCACAAAAGATAAATTTAAAAATATGGAGATTGCAATGAAAAAACAGGAAGCTCTTATGGCTTTGTTACTTGGGGCATATGAGTCAACTGATATGCTTGCTGCAGCTTTAAGAATGAAGGGTGCTTCTCTCCAACCATCGATGAGCCATATTCAATCTTCATATGAGGAGAACTTGGATGATGTTTATATACCACATTGCTCTGAAGAATTGCAGCAGCAAGTTACCAGAAATTGA

Protein Analysis

449

Amino Acids

52.36

Weight (kDa)

8.46

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transposase_24 PF03004 189 - 320 1e-22 Plant transposase (Ptta/En/Spm family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63250 AT3G63250 AT3G63250
fragaria_vesca FvH4_2g23130 FvH4_2g23130
malus_domestica MD03G1260400.v1.1 MD11G1280700.v1.1
prunus_persica Prupe.8G236600_v2.0.a1
pyrus_communis pycom03g20810 pycom11g24850
rosa_chinensis RchiOBHm_Chr6g0290381
rosa_laevigata RLG00000012224
rosa_multiflora Rmu_sc0025482.1_g000001
rosa_roxburghii Rroxscaffold_7G00177120
rosa_rugosa Rorug06G0212100 Rorug06G0212100 Rorug06G0212200
rosa_samantha Rh6DG322700
rosa_wichuraiana Rw6G027840 Rw6G027940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 521
AccI GTMKAC 1 cut(s) 509
AccIII TCCGGA 1 cut(s) 495
AcoI YGGCCR 1 cut(s) 1113
AcsI RAATTY 2 cut(s) 784, 1127
AcuI CTGAAG 4 cut(s) 159, 165, 549, 1338
AcvI CACGTG 1 cut(s) 923
AfaI GTAC 3 cut(s) 526, 978, 1011
AfiI CCNNNNNNNGG 2 cut(s) 521, 1072
AflIII ACRYGT 1 cut(s) 920
AgsI TTSAA 7 cut(s) 10, 220, 226, 463, 881, 1046, 1268
AhlI ACTAGT 2 cut(s) 583, 979
AjnI CCWGG 1 cut(s) 970
AjuI GAANNNNNNNTTGG 2 cut(s) 382, 414
AluBI AGCT 9 cut(s) 143, 159, 242, 319, 757, 797, 1084, 1166, 1220
AluI AGCT 9 cut(s) 143, 159, 242, 319, 757, 797, 1084, 1166, 1220
Alw26I GTCTC 2 cut(s) 857, 911
Aor13HI TCCGGA 1 cut(s) 495
AoxI GGCC 3 cut(s) 182, 294, 1113
ApeKI GCWGC 4 cut(s) 1214, 1217, 1327, 1330
ApoI RAATTY 2 cut(s) 784, 1127
Asp700I GAANNNNTTC 2 cut(s) 966, 1050
BalI TGGCCA 1 cut(s) 1115
BbrPI CACGTG 1 cut(s) 923
BbsI GAAGAC 2 cut(s) 482, 839
BbvI GCAGC 4 cut(s) 1201, 1229, 1339, 1342
BccI CCATC 4 cut(s) 119, 264, 545, 1258
BciT130I CCWGG 1 cut(s) 972
BcoDI GTCTC 2 cut(s) 857, 911
BcuI ACTAGT 2 cut(s) 583, 979
BfaI CTAG 3 cut(s) 362, 584, 980
BfmI CTRYAG 1 cut(s) 1215
BisI GCNGC 4 cut(s) 1215, 1218, 1328, 1331
BlsI GCNGC 4 cut(s) 1216, 1219, 1329, 1332
Bme1390I CCNGG 1 cut(s) 972
BmiI GGNNCC 2 cut(s) 18, 249
BmrFI CCNGG 1 cut(s) 972
BmrI ACTGGG 1 cut(s) 604
BmsI GCATC 3 cut(s) 367, 651, 857
BmuI ACTGGG 1 cut(s) 604
BpiI GAAGAC 2 cut(s) 482, 839
Bpu10I CCTNAGC 1 cut(s) 1085
Bsa29I ATCGAT 1 cut(s) 1253
BsaAI YACGTR 1 cut(s) 923
BsaJI CCNNGG 1 cut(s) 283
BsaWI WCCGGW 1 cut(s) 495
BsaXI ACNNNNNCTCC 4 cut(s) 192, 222, 443, 473
Bsc4I CCNNNNNNNGG 2 cut(s) 521, 1072
Bse1I ACTGG 2 cut(s) 599, 676
Bse3DI GCAATG 4 cut(s) 34, 1023, 1155, 1309
BseAI TCCGGA 1 cut(s) 495
BseBI CCWGG 1 cut(s) 972
BseCI ATCGAT 1 cut(s) 1253
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 6 cut(s) 130, 157, 382, 663, 848, 1297
BseLI CCNNNNNNNGG 2 cut(s) 521, 1072
BseMI GCAATG 4 cut(s) 34, 1023, 1155, 1309
BseMII CTCAG 1 cut(s) 1029
BseNI ACTGG 2 cut(s) 599, 676
BseRI GAGGAG 1 cut(s) 1295
BseXI GCAGC 4 cut(s) 1201, 1229, 1339, 1342
BsgI GTGCAG 1 cut(s) 1004
BshFI GGCC 3 cut(s) 184, 296, 1115
BshVI ATCGAT 1 cut(s) 1253
BsiSI CCGG 1 cut(s) 496
BslI CCNNNNNNNGG 2 cut(s) 521, 1072
BsmAI GTCTC 2 cut(s) 857, 911
BsnI GGCC 3 cut(s) 184, 296, 1115
Bsp13I TCCGGA 1 cut(s) 495
Bsp143I GATC 3 cut(s) 301, 466, 693
BspANI GGCC 3 cut(s) 184, 296, 1115
BspCNI CTCAG 1 cut(s) 1030
BspDI ATCGAT 1 cut(s) 1253
BspEI TCCGGA 1 cut(s) 495
BspLI GGNNCC 2 cut(s) 18, 249
BspMAI CTGCAG 1 cut(s) 1219
BspQI GCTCTTC 1 cut(s) 525
BsrDI GCAATG 4 cut(s) 34, 1023, 1155, 1309
BsrI ACTGG 2 cut(s) 599, 676
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 3 cut(s) 301, 466, 693
BssNAI GTATAC 1 cut(s) 510
BssT1I CCWWGG 1 cut(s) 283
Bst1107I GTATAC 1 cut(s) 510
Bst2UI CCWGG 1 cut(s) 972
Bst4CI ACNGT 3 cut(s) 262, 615, 740
Bst6I CTCTTC 3 cut(s) 141, 525, 983
BstAPI GCANNNNNTGC 2 cut(s) 263, 377
BstBAI YACGTR 1 cut(s) 923
BstC8I GCNNGC 2 cut(s) 240, 1212
BstDEI CTNAG 3 cut(s) 23, 1038, 1085
BstF5I GGATG 6 cut(s) 130, 157, 382, 663, 848, 1297
BstKTI GATC 3 cut(s) 304, 469, 696
BstMAI GTCTC 2 cut(s) 857, 911
BstMBI GATC 3 cut(s) 301, 466, 693
BstMWI GCNNNNNNNGC 4 cut(s) 61, 263, 377, 1172
BstNI CCWGG 1 cut(s) 972
BstNSI RCATGY 1 cut(s) 64
BstSCI CCNGG 1 cut(s) 970
BstSFI CTRYAG 1 cut(s) 1215
BstV1I GCAGC 4 cut(s) 1201, 1229, 1339, 1342
BstV2I GAAGAC 2 cut(s) 482, 839
BstZ17I GTATAC 1 cut(s) 510
Bsu15I ATCGAT 1 cut(s) 1253
BsuRI GGCC 3 cut(s) 184, 296, 1115
BsuTUI ATCGAT 1 cut(s) 1253
BtsCI GGATG 6 cut(s) 130, 157, 382, 663, 848, 1297
BtsIMutI CAGTG 2 cut(s) 592, 620
Cac8I GCNNGC 2 cut(s) 240, 1212
ClaI ATCGAT 1 cut(s) 1253
Csp6I GTAC 3 cut(s) 525, 977, 1010
CviAII CATG 5 cut(s) 61, 289, 372, 764, 1031
CviQI GTAC 3 cut(s) 525, 977, 1010
DdeI CTNAG 3 cut(s) 23, 1038, 1085
DpnI GATC 3 cut(s) 303, 468, 695
DpnII GATC 3 cut(s) 301, 466, 693
DraI TTTAAA 2 cut(s) 541, 1132
EaeI YGGCCR 1 cut(s) 1113
Eam1104I CTCTTC 3 cut(s) 141, 525, 983
EarI CTCTTC 3 cut(s) 141, 525, 983
Eco130I CCWWGG 1 cut(s) 283
Eco57I CTGAAG 4 cut(s) 159, 165, 549, 1338
Eco72I CACGTG 1 cut(s) 923
EcoRII CCWGG 1 cut(s) 970
EcoT14I CCWWGG 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 5 cut(s) 64, 292, 375, 767, 1034
FalI AAGNNNNNCTT 2 cut(s) 1068, 1100
FatI CATG 5 cut(s) 60, 288, 371, 763, 1030
FauNDI CATATG 2 cut(s) 1192, 1276
FblI GTMKAC 1 cut(s) 509
Fnu4HI GCNGC 4 cut(s) 1215, 1218, 1328, 1331
FokI GGATG 6 cut(s) 137, 164, 389, 670, 835, 1304
Fsp4HI GCNGC 4 cut(s) 1215, 1218, 1328, 1331
FspBI CTAG 3 cut(s) 362, 584, 980
GluI GCNGC 4 cut(s) 1215, 1218, 1328, 1331
HaeIII GGCC 3 cut(s) 184, 296, 1115
HapII CCGG 1 cut(s) 496
Hin1II CATG 5 cut(s) 64, 292, 375, 767, 1034
HincII GTYRAC 2 cut(s) 589, 1200
HindII GTYRAC 2 cut(s) 589, 1200
HindIII AAGCTT 1 cut(s) 1082
HinfI GANTC 5 cut(s) 640, 865, 951, 1051, 1196
HpaI GTTAAC 1 cut(s) 589
HpaII CCGG 1 cut(s) 496
Hpy166II GTNNAC 8 cut(s) 120, 510, 589, 631, 730, 769, 977, 1200
Hpy188I TCNGA 5 cut(s) 139, 406, 956, 1056, 1318
Hpy188III TCNNGA 4 cut(s) 385, 496, 697, 860
Hpy8I GTNNAC 8 cut(s) 120, 510, 589, 631, 730, 769, 977, 1200
HpyAV CCTTC 5 cut(s) 227, 286, 805, 1051, 1225
HpyCH4III ACNGT 3 cut(s) 262, 615, 740
HpyCH4IV ACGT 1 cut(s) 922
HpyF10VI GCNNNNNNNGC 4 cut(s) 61, 263, 377, 1172
HpyF3I CTNAG 3 cut(s) 23, 1038, 1085
HpySE526I ACGT 1 cut(s) 922
Hsp92II CATG 5 cut(s) 64, 292, 375, 767, 1034
Kpn2I TCCGGA 1 cut(s) 495
KspAI GTTAAC 1 cut(s) 589
Kzo9I GATC 3 cut(s) 301, 466, 693
LguI GCTCTTC 1 cut(s) 525
LmnI GCTCC 2 cut(s) 16, 247
Lsp1109I GCAGC 4 cut(s) 1201, 1229, 1339, 1342
LweI GCATC 3 cut(s) 367, 651, 857
MaeI CTAG 3 cut(s) 362, 584, 980
MaeII ACGT 1 cut(s) 922
MaeIII GTNAC 2 cut(s) 1179, 1336
MalI GATC 3 cut(s) 303, 468, 695
MboI GATC 3 cut(s) 301, 466, 693
MlsI TGGCCA 1 cut(s) 1115
MluCI AATT 6 cut(s) 163, 187, 784, 1127, 1322, 1345
MluNI TGGCCA 1 cut(s) 1115
MlyI GAGTC 4 cut(s) 649, 859, 960, 1205
MmeI TCCRAC 2 cut(s) 452, 1270
Mox20I TGGCCA 1 cut(s) 1115
MroI TCCGGA 1 cut(s) 495
MroXI GAANNNNTTC 2 cut(s) 966, 1050
MscI TGGCCA 1 cut(s) 1115
MseI TTAA 8 cut(s) 162, 483, 540, 588, 806, 870, 1131, 1223
MslI CAYNNNNRTG 1 cut(s) 774
Msp20I TGGCCA 1 cut(s) 1115
MspI CCGG 1 cut(s) 496
MspR9I CCNGG 1 cut(s) 972
MvaI CCWGG 1 cut(s) 972
MwoI GCNNNNNNNGC 4 cut(s) 61, 263, 377, 1172
NdeI CATATG 2 cut(s) 1192, 1276
NdeII GATC 3 cut(s) 301, 466, 693
NlaIII CATG 5 cut(s) 64, 292, 375, 767, 1034
NlaIV GGNNCC 2 cut(s) 18, 249
NspI RCATGY 1 cut(s) 64
PciSI GCTCTTC 1 cut(s) 525
PcsI WCGNNNNNNNCGW 2 cut(s) 128, 649
PdmI GAANNNNTTC 2 cut(s) 966, 1050
PfeI GAWTC 1 cut(s) 1051
PflMI CCANNNNNTGG 1 cut(s) 521
PkrI GCNGC 4 cut(s) 1216, 1219, 1329, 1332
PleI GAGTC 4 cut(s) 648, 859, 959, 1204
PmaCI CACGTG 1 cut(s) 923
PmlI CACGTG 1 cut(s) 923
PpsI GAGTC 4 cut(s) 648, 859, 959, 1204
Ppu21I YACGTR 1 cut(s) 923
Psp6I CCWGG 1 cut(s) 970
PspCI CACGTG 1 cut(s) 923
PspGI CCWGG 1 cut(s) 970
PspN4I GGNNCC 2 cut(s) 18, 249
PstI CTGCAG 1 cut(s) 1219
RsaI GTAC 3 cut(s) 526, 978, 1011
RsaNI GTAC 3 cut(s) 525, 977, 1010
RseI CAYNNNNRTG 1 cut(s) 774
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 8 cut(s) 162, 483, 540, 588, 806, 870, 1131, 1223
SatI GCNGC 4 cut(s) 1215, 1218, 1328, 1331
Sau3AI GATC 3 cut(s) 301, 466, 693
SchI GAGTC 4 cut(s) 649, 859, 960, 1205
ScrFI CCNGG 1 cut(s) 972
SfaNI GCATC 3 cut(s) 367, 651, 857
SfcI CTRYAG 1 cut(s) 1215
SmiMI CAYNNNNRTG 1 cut(s) 774
SpeI ACTAGT 2 cut(s) 583, 979
Sse9I AATT 6 cut(s) 163, 187, 784, 1127, 1322, 1345
SspMI CTAG 3 cut(s) 362, 584, 980
StyD4I CCNGG 1 cut(s) 970
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 3 cut(s) 262, 615, 740
TaiI ACGT 1 cut(s) 925
TaqI TCGA 2 cut(s) 131, 1253
TasI AATT 6 cut(s) 163, 187, 784, 1127, 1322, 1345
TatI WGTACW 1 cut(s) 976
TfiI GAWTC 1 cut(s) 1051
Tru1I TTAA 8 cut(s) 162, 483, 540, 588, 806, 870, 1131, 1223
Tru9I TTAA 8 cut(s) 162, 483, 540, 588, 806, 870, 1131, 1223
TscAI CASTG 2 cut(s) 599, 620
TseI GCWGC 4 cut(s) 1214, 1217, 1327, 1330
TspGWI ACGGA 1 cut(s) 137
TspRI CASTG 2 cut(s) 599, 620
Van91I CCANNNNNTGG 1 cut(s) 521
XapI RAATTY 2 cut(s) 784, 1127
XceI RCATGY 1 cut(s) 64
XmiI GTMKAC 1 cut(s) 509
XmnI GAANNNNTTC 2 cut(s) 966, 1050
XspI CTAG 3 cut(s) 362, 584, 980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.