MD11G1280700.v1.1

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010707
Physical Location & Seq
Forward (+)
39840013 .. 39843134
3122 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1280700.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGGCAGCAGCAGCAGCGCTAAAACGCCGTCGTTAATGAGGGACTTTCTCAGGCAGGCGGGCGGCATCGCGGTGGTCGACGGTGGACTTGCCACCGAGCTCGAACGCCACGGTGCTGACCTCAACGATCCTCTCTGGAGCGCCAAATGCCTTCTCACTTCCCCTCACCTCATTCGCACGTTACACATTGAATACCTTGAAGCTGGTGCAGATATCATAATCACAGCGTCCTATCAGGCCACCATTCAAGGTTTCGAGGCCAAAGGATATTCAACAGAAGAGAGTGAAGCCTTGCTTCGGAAAAGTGTTGAAATTGCCCGTGAGGCACGTGATGTGTATTACGACAGATGTACTCAATGCTCTTCTGCTGACAGTGCGAATGGTAGAATTCTCAAGCGTAGGCCAATCTTAGTTGCAGCATCTGTGGGGAGCTATGGTGCTTATTTGGCTGATGGGTCTGAGTACAGTGGAGATTATGGTGAGGCTATGACGCTAGGAAGATTAAAAGATTTTCATCGGAGAAGGGTCCAGGTCCTAGCAGCATCGGGTCCTGACCTACTTGCATTTGAAACGGTTCCAAATAAGCTGGAGGCTCAGGCTTATGCTGAGCTTTTGGAAGAAGAAAATATGCAACTTCCTGCGTGGTTTTCTTTCAACTCTAAAGATGGCATCAATGTTGTTAGTGGTGATTCTCTCCTTGAATGCGCCTCAGTTGCTGAATCATGCAAGAAAGTCGTTGCAGTTGGAATCAACTGCACCCCCCCTAGATTTATCCATGGACTGTTAACATTAATTACACAGGTGGCTACAAAACCAATTATTGTGTACCCAAACAGTGGTGAAAGCTATGATGCAGATAGAATGATGTGGGTGCAAAACACTGGGGTTTCAGATGAAGATTTTGTCTCGTATGTAAACAAATGGTGTGAGGTAGGGGCTTCCCTTGTAGGAGGTTGTTGCAGAACAACCCCAAATACAATCAGAGCAATATACAGGACTCTTCCCAATAGGTCAACATCTCCACCTGATCTGTAA

Protein Analysis

345

Amino Acids

37.41

Weight (kDa)

5.2

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 25 - 333 7.9e-80 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63250 AT3G63250 AT3G63250
fragaria_vesca FvH4_2g23130 FvH4_2g23130
malus_domestica MD03G1260400.v1.1 MD11G1280700.v1.1
prunus_persica Prupe.8G236600_v2.0.a1
pyrus_communis pycom03g20810 pycom11g24850
rosa_chinensis RchiOBHm_Chr6g0290381
rosa_laevigata RLG00000012224
rosa_multiflora Rmu_sc0025482.1_g000001
rosa_roxburghii Rroxscaffold_7G00177120
rosa_rugosa Rorug06G0212100 Rorug06G0212100 Rorug06G0212200
rosa_samantha Rh6DG322700
rosa_wichuraiana Rw6G027840 Rw6G027940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 836
AccI GTMKAC 1 cut(s) 78
AccII CGCG 1 cut(s) 71
AciI CCGC 3 cut(s) 59, 63, 71
AclWI GGATC 1 cut(s) 122
AcsI RAATTY 1 cut(s) 387
AcvI CACGTG 1 cut(s) 329
AfaI GTAC 3 cut(s) 352, 464, 827
AfeI AGCGCT 1 cut(s) 19
AfiI CCNNNNNNNGG 2 cut(s) 297, 836
AgsI TTSAA 8 cut(s) 191, 200, 248, 273, 311, 569, 655, 701
AjnI CCWGG 1 cut(s) 528
AluBI AGCT 6 cut(s) 100, 203, 432, 586, 610, 846
AluI AGCT 6 cut(s) 100, 203, 432, 586, 610, 846
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 910
AlwI GGATC 1 cut(s) 122
AlwNI CAGNNNCTG 2 cut(s) 422, 716
Aor51HI AGCGCT 1 cut(s) 19
AoxI GGCC 3 cut(s) 237, 258, 401
ApeKI GCWGC 6 cut(s) 6, 9, 12, 15, 416, 539
ApoI RAATTY 1 cut(s) 387
AseI ATTAAT 1 cut(s) 791
Asp700I GAANNNNTTC 1 cut(s) 573
AspLEI GCGC 3 cut(s) 20, 143, 707
AspS9I GGNCC 3 cut(s) 526, 532, 548
AsuHPI GGTGA 4 cut(s) 158, 491, 698, 851
AvaII GGWCC 3 cut(s) 526, 532, 548
BanII GRGCYC 1 cut(s) 102
BbrPI CACGTG 1 cut(s) 329
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 6 cut(s) 18, 21, 24, 27, 428, 551
BccI CCATC 2 cut(s) 446, 659
BceAI ACGGC 1 cut(s) 13
BcgI CGANNNNNNTGC 2 cut(s) 715, 749
BciT130I CCWGG 1 cut(s) 530
BcoDI GTCTC 1 cut(s) 910
BfaI CTAG 3 cut(s) 494, 536, 765
BfoI RGCGCY 2 cut(s) 21, 144
BglI GCCNNNNNGGC 1 cut(s) 323
BisI GCNGC 7 cut(s) 7, 10, 13, 16, 64, 417, 540
BlpI GCTNAGC 1 cut(s) 606
BlsI GCNGC 7 cut(s) 8, 11, 14, 17, 65, 418, 541
Bme1390I CCNGG 1 cut(s) 530
Bme18I GGWCC 3 cut(s) 526, 532, 548
BmgT120I GGNCC 3 cut(s) 526, 532, 548
BmiI GGNNCC 3 cut(s) 527, 549, 576
BmrFI CCNGG 1 cut(s) 530
BmrI ACTGGG 1 cut(s) 891
BmsI GCATC 5 cut(s) 75, 428, 551, 678, 841
BmuI ACTGGG 1 cut(s) 891
BpmI CTGGAG 2 cut(s) 157, 608
Bpu10I CCTNAGC 1 cut(s) 594
Bpu1102I GCTNAGC 1 cut(s) 606
BpuEI CTTGAG 1 cut(s) 377
BsaAI YACGTR 1 cut(s) 329
BsaBI GATNNNNATC 1 cut(s) 513
BsaJI CCNNGG 2 cut(s) 109, 775
BsaXI ACNNNNNCTCC 2 cut(s) 421, 451
Bsc4I CCNNNNNNNGG 2 cut(s) 297, 836
Bse1I ACTGG 1 cut(s) 886
Bse8I GATNNNNATC 1 cut(s) 513
BseBI CCWGG 1 cut(s) 530
BseDI CCNNGG 2 cut(s) 109, 775
BseJI GATNNNNATC 1 cut(s) 513
BseLI CCNNNNNNNGG 2 cut(s) 297, 836
BseMII CTCAG 5 cut(s) 64, 450, 597, 608, 723
BseNI ACTGG 1 cut(s) 886
BseXI GCAGC 6 cut(s) 18, 21, 24, 27, 428, 551
BsgI GTGCAG 2 cut(s) 228, 739
Bsh1236I CGCG 1 cut(s) 71
BshFI GGCC 3 cut(s) 239, 260, 403
BsiHKAI GWGCWC 1 cut(s) 102
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 297, 836
BsmAI GTCTC 1 cut(s) 910
BsmFI GGGAC 1 cut(s) 56
BsmI GAATGC 1 cut(s) 707
BsnI GGCC 3 cut(s) 239, 260, 403
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 2 cut(s) 127, 1027
Bsp1720I GCTNAGC 1 cut(s) 606
Bsp19I CCATGG 1 cut(s) 775
BspACI CCGC 3 cut(s) 59, 63, 71
BspANI GGCC 3 cut(s) 239, 260, 403
BspCNI CTCAG 5 cut(s) 63, 451, 598, 607, 722
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 3 cut(s) 527, 549, 576
BspPI GGATC 1 cut(s) 122
BspQI GCTCTTC 1 cut(s) 367
BsrI ACTGG 1 cut(s) 886
BssECI CCNNGG 2 cut(s) 109, 775
BssMI GATC 2 cut(s) 127, 1027
BssT1I CCWWGG 1 cut(s) 775
Bst2UI CCWGG 1 cut(s) 530
Bst4CI ACNGT 7 cut(s) 83, 113, 374, 467, 574, 783, 836
Bst6I CTCTTC 3 cut(s) 273, 367, 1005
BstBAI YACGTR 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 57, 61
BstDEI CTNAG 6 cut(s) 50, 409, 459, 594, 606, 709
BstDSI CCRYGG 2 cut(s) 109, 775
BstFNI CGCG 1 cut(s) 71
BstH2I RGCGCY 2 cut(s) 21, 144
BstHHI GCGC 3 cut(s) 20, 143, 707
BstKTI GATC 2 cut(s) 130, 1030
BstMAI GTCTC 1 cut(s) 910
BstMBI GATC 2 cut(s) 127, 1027
BstMWI GCNNNNNNNGC 7 cut(s) 12, 15, 147, 323, 374, 446, 713
BstNI CCWGG 1 cut(s) 530
BstSCI CCNGG 1 cut(s) 528
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 6 cut(s) 18, 21, 24, 27, 428, 551
BsuRI GGCC 3 cut(s) 239, 260, 403
BtgI CCRYGG 2 cut(s) 109, 775
BtgZI GCGATG 1 cut(s) 52
BtsIMutI CAGTG 4 cut(s) 379, 472, 841, 879
Cac8I GCNNGC 2 cut(s) 57, 61
CaiI CAGNNNCTG 2 cut(s) 422, 716
CfoI GCGC 3 cut(s) 20, 143, 707
Cfr13I GGNCC 3 cut(s) 526, 532, 548
CseI GACGC 2 cut(s) 216, 499
Csp6I GTAC 3 cut(s) 351, 463, 826
CviAII CATG 2 cut(s) 723, 776
CviQI GTAC 3 cut(s) 351, 463, 826
DdeI CTNAG 6 cut(s) 50, 409, 459, 594, 606, 709
DpnI GATC 2 cut(s) 129, 1029
DpnII GATC 2 cut(s) 127, 1027
Eam1104I CTCTTC 3 cut(s) 273, 367, 1005
EarI CTCTTC 3 cut(s) 273, 367, 1005
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 775
Eco24I GRGCYC 1 cut(s) 102
Eco32I GATATC 1 cut(s) 214
Eco47I GGWCC 3 cut(s) 526, 532, 548
Eco47III AGCGCT 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 100
Eco72I CACGTG 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 100
EcoO109I RGGNCCY 2 cut(s) 532, 548
EcoRI GAATTC 1 cut(s) 387
EcoRII CCWGG 1 cut(s) 528
EcoRV GATATC 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 775
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 775
FaeI CATG 2 cut(s) 726, 779
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FaqI GGGAC 1 cut(s) 56
FatI CATG 2 cut(s) 722, 775
FauI CCCGC 1 cut(s) 52
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 7 cut(s) 7, 10, 13, 16, 64, 417, 540
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 7 cut(s) 7, 10, 13, 16, 64, 417, 540
FspBI CTAG 3 cut(s) 494, 536, 765
GlaI GCGC 3 cut(s) 19, 142, 706
GluI GCNGC 7 cut(s) 7, 10, 13, 16, 64, 417, 540
GsuI CTGGAG 2 cut(s) 157, 608
HaeII RGCGCY 2 cut(s) 21, 144
HaeIII GGCC 3 cut(s) 239, 260, 403
HgaI GACGC 2 cut(s) 216, 499
HhaI GCGC 3 cut(s) 20, 143, 707
Hin1II CATG 2 cut(s) 726, 779
Hin6I GCGC 3 cut(s) 18, 141, 705
HinP1I GCGC 3 cut(s) 18, 141, 705
HincII GTYRAC 3 cut(s) 79, 786, 1014
HindII GTYRAC 3 cut(s) 79, 786, 1014
HinfI GANTC 4 cut(s) 689, 719, 747, 997
HpaI GTTAAC 1 cut(s) 786
HphI GGTGA 4 cut(s) 158, 491, 698, 851
Hpy166II GTNNAC 6 cut(s) 79, 86, 786, 826, 916, 1014
Hpy188I TCNGA 5 cut(s) 300, 460, 519, 892, 983
Hpy188III TCNNGA 2 cut(s) 136, 551
Hpy8I GTNNAC 6 cut(s) 79, 86, 786, 826, 916, 1014
Hpy99I CGWCG 2 cut(s) 34, 83
HpyAV CCTTC 2 cut(s) 161, 516
HpyCH4III ACNGT 7 cut(s) 83, 113, 374, 467, 574, 783, 836
HpyCH4IV ACGT 2 cut(s) 179, 328
HpyF10VI GCNNNNNNNGC 7 cut(s) 12, 15, 147, 323, 374, 446, 713
HpyF3I CTNAG 6 cut(s) 50, 409, 459, 594, 606, 709
HpySE526I ACGT 2 cut(s) 179, 328
Hsp92II CATG 2 cut(s) 726, 779
HspAI GCGC 3 cut(s) 18, 141, 705
KspAI GTTAAC 1 cut(s) 786
Kzo9I GATC 2 cut(s) 127, 1027
LguI GCTCTTC 1 cut(s) 367
LmnI GCTCC 2 cut(s) 138, 429
Lsp1109I GCAGC 6 cut(s) 18, 21, 24, 27, 428, 551
LweI GCATC 5 cut(s) 75, 428, 551, 678, 841
MaeI CTAG 3 cut(s) 494, 536, 765
MaeII ACGT 2 cut(s) 179, 328
MaeIII GTNAC 1 cut(s) 180
MalI GATC 2 cut(s) 129, 1029
MboI GATC 2 cut(s) 127, 1027
MboII GAAGA 7 cut(s) 290, 354, 510, 629, 632, 908, 992
MhlI GDGCHC 1 cut(s) 102
MluCI AATT 4 cut(s) 312, 387, 792, 816
MlyI GAGTC 1 cut(s) 991
MmeI TCCRAC 1 cut(s) 724
MroXI GAANNNNTTC 1 cut(s) 573
MseI TTAA 4 cut(s) 35, 503, 785, 791
MslI CAYNNNNRTG 1 cut(s) 71
MspR9I CCNGG 1 cut(s) 530
Mva1269I GAATGC 1 cut(s) 707
MvaI CCWGG 1 cut(s) 530
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 7 cut(s) 12, 15, 147, 323, 374, 446, 713
NcoI CCATGG 1 cut(s) 775
NdeII GATC 2 cut(s) 127, 1027
NlaIII CATG 2 cut(s) 726, 779
NlaIV GGNNCC 3 cut(s) 527, 549, 576
PciSI GCTCTTC 1 cut(s) 367
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 707
PdmI GAANNNNTTC 1 cut(s) 573
PfeI GAWTC 3 cut(s) 689, 719, 747
PflMI CCANNNNNTGG 1 cut(s) 836
PkrI GCNGC 7 cut(s) 8, 11, 14, 17, 65, 418, 541
PleI GAGTC 1 cut(s) 991
PmaCI CACGTG 1 cut(s) 329
PmlI CACGTG 1 cut(s) 329
PpsI GAGTC 1 cut(s) 991
Ppu21I YACGTR 1 cut(s) 329
PpuMI RGGWCCY 2 cut(s) 532, 548
PshBI ATTAAT 1 cut(s) 791
Psp124BI GAGCTC 1 cut(s) 102
Psp5II RGGWCCY 2 cut(s) 532, 548
Psp6I CCWGG 1 cut(s) 528
PspCI CACGTG 1 cut(s) 329
PspGI CCWGG 1 cut(s) 528
PspN4I GGNNCC 3 cut(s) 527, 549, 576
PspPI GGNCC 3 cut(s) 526, 532, 548
PspPPI RGGWCCY 2 cut(s) 532, 548
PstNI CAGNNNCTG 2 cut(s) 422, 716
RsaI GTAC 3 cut(s) 352, 464, 827
RsaNI GTAC 3 cut(s) 351, 463, 826
RseI CAYNNNNRTG 1 cut(s) 71
SacI GAGCTC 1 cut(s) 102
SalI GTCGAC 1 cut(s) 77
SapI GCTCTTC 1 cut(s) 367
SaqAI TTAA 4 cut(s) 35, 503, 785, 791
SatI GCNGC 7 cut(s) 7, 10, 13, 16, 64, 417, 540
Sau3AI GATC 2 cut(s) 127, 1027
Sau96I GGNCC 3 cut(s) 526, 532, 548
SchI GAGTC 1 cut(s) 991
ScrFI CCNGG 1 cut(s) 530
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 5 cut(s) 75, 428, 551, 678, 841
SinI GGWCC 3 cut(s) 526, 532, 548
SmiMI CAYNNNNRTG 1 cut(s) 71
SmlI CTYRAG 1 cut(s) 392
SmoI CTYRAG 1 cut(s) 392
Sse9I AATT 4 cut(s) 312, 387, 792, 816
SsiI CCGC 3 cut(s) 59, 63, 71
SspMI CTAG 3 cut(s) 494, 536, 765
SstI GAGCTC 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 528
StyI CCWWGG 1 cut(s) 775
TaaI ACNGT 7 cut(s) 83, 113, 374, 467, 574, 783, 836
TaiI ACGT 2 cut(s) 182, 331
TaqI TCGA 3 cut(s) 78, 102, 255
TasI AATT 4 cut(s) 312, 387, 792, 816
TatI WGTACW 2 cut(s) 350, 462
TauI GCSGC 1 cut(s) 66
TfiI GAWTC 3 cut(s) 689, 719, 747
Tru1I TTAA 4 cut(s) 35, 503, 785, 791
Tru9I TTAA 4 cut(s) 35, 503, 785, 791
TscAI CASTG 4 cut(s) 379, 472, 841, 886
TseI GCWGC 6 cut(s) 6, 9, 12, 15, 416, 539
TspDTI ATGAA 2 cut(s) 503, 909
TspRI CASTG 4 cut(s) 379, 472, 841, 886
Van91I CCANNNNNTGG 1 cut(s) 836
VpaK11BI GGWCC 3 cut(s) 526, 532, 548
VspI ATTAAT 1 cut(s) 791
XapI RAATTY 1 cut(s) 387
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 573
XspI CTAG 3 cut(s) 494, 536, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.