Rroxscaffold_7G00177120

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010707
Physical Location & Seq
Reverse (-)
16662435 .. 16665176
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00177120.1

Sequence Viewer

Length: 1035 bp
ATGGGCAGCCGCAGCAGCGTCGAAACGGCGTCGTCGTCAATGAGGGAGTTTCTGCGGCAGGCCGGCGGCGTCGCCGTCGTCGACGGTGGTCTGGCGACCGAGCTCGAGCGGCACGGCGCTGACCTCAACGATCCTCTCTGGAGCGCCAAGTGCCTCCTCACTTCCTCTCACCTCATTCGCACCGTTCATCTTGATTATCTTGAAGCTGGTGCTGATATCATAATCACAGCTTCTTATCAGGCAACTATTCAAGGTTTTGAGGCCAAAGGGTTTTCGACTGAGGAGAGTGAATCATTGCTTAGGAAGAGTGTGGAGATTGCGCGTGAGGCGCGAGATATTTATTACGAAAGGTGTAGTGAGGGGTCTTGTAATGAGAGTGATGCTAGAGTTCTCAAACGTCGGCCGATCATAGTTGCAGCATCTGTGGGGAGCTATGGTGCTTATTTGGCTGATGGGTCTGAATACAGTGGTGATTATGGTGAGGCTATGACGCTGGGAGCTTTGAAAGAGTTTCATCGTAGAAGAGTCCAAGTCCTAACAGAAGCAGGTCCTGACCTACTTGCATTTGAAACTATTCCAAATAAGCTGGAAGCCCAGGCTTTTGCTGAGCTTTTGGAAGAAGAAGACATAAAGCTTCCTGCATGGTTTTGTTTCAACTCTAAAGATGGAGTCAATGTTGTTAGTGGTGATTCTTTCCTTGAATGTGCCTCGGTTGCTGAATCCTGTAAGAAAGTTGTTGCTGTGGGGATTAACTGTACTCCTCCTAGATTTATTCATGGACTGATCACAACAATTAAAAAGGTGGCTACAAAACCAATAGTTATATACCCAAATAGTGGCGAAAGTTATGATGCTGATCGAAAGGAGTGGGTACAATATACTGGGGTTTCAGATGAAGATTTTGTTTCATATGTAAACAAATGGTGTGAGGCAGGGGCTTCCCTTGTGGGAGGTTGTTGCAGAACAACTCCAAATACTATCAGGGCCATCTACAAAACCCTTCCCAATCGGTCAACATCTCCACCTGAACAGTAA

Protein Analysis

344

Amino Acids

37.53

Weight (kDa)

5.07

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 26 - 333 3.6e-82 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63250 AT3G63250 AT3G63250
fragaria_vesca FvH4_2g23130 FvH4_2g23130
malus_domestica MD03G1260400.v1.1 MD11G1280700.v1.1
prunus_persica Prupe.8G236600_v2.0.a1
pyrus_communis pycom03g20810 pycom11g24850
rosa_chinensis RchiOBHm_Chr6g0290381
rosa_laevigata RLG00000012224
rosa_multiflora Rmu_sc0025482.1_g000001
rosa_roxburghii Rroxscaffold_7G00177120
rosa_rugosa Rorug06G0212100 Rorug06G0212100 Rorug06G0212200
rosa_samantha Rh6DG322700
rosa_wichuraiana Rw6G027840 Rw6G027940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 536
AccB7I CCANNNNNTGG 1 cut(s) 836
AccBSI CCGCTC 1 cut(s) 109
AccI GTMKAC 1 cut(s) 81
AccII CGCG 2 cut(s) 322, 331
AciI CCGC 4 cut(s) 10, 55, 66, 109
AclWI GGATC 1 cut(s) 125
AcoI YGGCCR 1 cut(s) 401
AcyI GRCGYC 2 cut(s) 29, 69
AfaI GTAC 2 cut(s) 757, 873
AfiI CCNNNNNNNGG 1 cut(s) 836
AgsI TTSAA 6 cut(s) 203, 251, 505, 569, 655, 701
AjnI CCWGG 1 cut(s) 594
AluBI AGCT 8 cut(s) 103, 206, 230, 432, 500, 586, 610, 634
AluI AGCT 8 cut(s) 103, 206, 230, 432, 500, 586, 610, 634
Alw21I GWGCWC 1 cut(s) 105
AlwI GGATC 1 cut(s) 125
AlwNI CAGNNNCTG 2 cut(s) 422, 551
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 4 cut(s) 60, 261, 401, 984
ApeKI GCWGC 4 cut(s) 6, 12, 15, 416
Asp700I GAANNNNTTC 1 cut(s) 573
AspLEI GCGC 4 cut(s) 119, 146, 322, 331
AspS9I GGNCC 2 cut(s) 548, 984
AsuHPI GGTGA 4 cut(s) 161, 482, 491, 698
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 548
BanII GRGCYC 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 105
BbvI GCAGC 4 cut(s) 18, 24, 27, 428
BccI CCATC 3 cut(s) 446, 659, 995
BceAI ACGGC 3 cut(s) 42, 59, 130
BcgI CGANNNNNNTGC 1 cut(s) 35
BciT130I CCWGG 1 cut(s) 596
BclI TGATCA 1 cut(s) 783
BfaI CTAG 2 cut(s) 384, 765
BfoI RGCGCY 2 cut(s) 120, 147
BfuAI ACCTGC 1 cut(s) 536
BisI GCNGC 8 cut(s) 7, 10, 13, 16, 56, 67, 110, 417
BlpI GCTNAGC 1 cut(s) 606
BlsI GCNGC 8 cut(s) 8, 11, 14, 17, 57, 68, 111, 418
Bme1390I CCNGG 1 cut(s) 596
Bme18I GGWCC 1 cut(s) 548
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 2 cut(s) 548, 984
BmrFI CCNGG 1 cut(s) 596
BmrI ACTGGG 1 cut(s) 891
BmsI GCATC 3 cut(s) 370, 428, 841
BmuI ACTGGG 1 cut(s) 891
BoxI GACNNNNGTC 1 cut(s) 87
BpiI GAAGAC 1 cut(s) 630
BpmI CTGGAG 1 cut(s) 160
Bpu10I CCTNAGC 1 cut(s) 299
Bpu1102I GCTNAGC 1 cut(s) 606
BsaBI GATNNNNATC 1 cut(s) 855
BsaHI GRCGYC 2 cut(s) 29, 69
BsaJI CCNNGG 2 cut(s) 594, 708
BsaXI ACNNNNNCTCC 4 cut(s) 421, 451, 660, 690
Bsc4I CCNNNNNNNGG 1 cut(s) 836
Bse118I RCCGGY 1 cut(s) 62
Bse1I ACTGG 1 cut(s) 886
Bse3DI GCAATG 1 cut(s) 293
Bse8I GATNNNNATC 1 cut(s) 855
BseBI CCWGG 1 cut(s) 596
BseDI CCNNGG 2 cut(s) 594, 708
BseJI GATNNNNATC 1 cut(s) 855
BseLI CCNNNNNNNGG 1 cut(s) 836
BseMI GCAATG 1 cut(s) 293
BseMII CTCAG 2 cut(s) 270, 597
BseNI ACTGG 1 cut(s) 886
BseRI GAGGAG 3 cut(s) 146, 296, 750
BseX3I CGGCCG 1 cut(s) 401
BseXI GCAGC 4 cut(s) 18, 24, 27, 428
BseYI CCCAGC 1 cut(s) 493
Bsh1236I CGCG 2 cut(s) 322, 331
Bsh1285I CGRYCG 2 cut(s) 99, 404
BshFI GGCC 4 cut(s) 62, 263, 403, 986
BsiEI CGRYCG 2 cut(s) 99, 404
BsiHKAI GWGCWC 1 cut(s) 105
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 1 cut(s) 63
BslI CCNNNNNNNGG 1 cut(s) 836
BsnI GGCC 4 cut(s) 62, 263, 403, 986
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 4 cut(s) 130, 405, 783, 856
Bsp1720I GCTNAGC 1 cut(s) 606
BspACI CCGC 4 cut(s) 10, 55, 66, 109
BspANI GGCC 4 cut(s) 62, 263, 403, 986
BspCNI CTCAG 2 cut(s) 271, 598
BspFNI CGCG 2 cut(s) 322, 331
BspMI ACCTGC 1 cut(s) 536
BspPI GGATC 1 cut(s) 125
BsrBI CCGCTC 1 cut(s) 109
BsrDI GCAATG 1 cut(s) 293
BsrFI RCCGGY 1 cut(s) 62
BsrI ACTGG 1 cut(s) 886
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 2 cut(s) 594, 708
BssMI GATC 4 cut(s) 130, 405, 783, 856
BssNI GRCGYC 2 cut(s) 29, 69
Bst2UI CCWGG 1 cut(s) 596
Bst4CI ACNGT 5 cut(s) 86, 184, 467, 755, 1032
Bst6I CTCTTC 2 cut(s) 299, 517
BstACI GRCGYC 2 cut(s) 29, 69
BstC8I GCNNGC 2 cut(s) 60, 64
BstDEI CTNAG 3 cut(s) 279, 299, 606
BstFNI CGCG 2 cut(s) 322, 331
BstH2I RGCGCY 2 cut(s) 120, 147
BstHHI GCGC 4 cut(s) 119, 146, 322, 331
BstKTI GATC 4 cut(s) 133, 408, 786, 859
BstMBI GATC 4 cut(s) 130, 405, 783, 856
BstMCI CGRYCG 2 cut(s) 99, 404
BstMWI GCNNNNNNNGC 8 cut(s) 12, 15, 109, 150, 326, 328, 446, 713
BstNI CCWGG 1 cut(s) 596
BstPAI GACNNNNGTC 1 cut(s) 87
BstSCI CCNGG 1 cut(s) 594
BstUI CGCG 2 cut(s) 322, 331
BstV1I GCAGC 4 cut(s) 18, 24, 27, 428
BstV2I GAAGAC 1 cut(s) 630
BstZI CGGCCG 1 cut(s) 401
BsuRI GGCC 4 cut(s) 62, 263, 403, 986
BtsIMutI CAGTG 1 cut(s) 472
BveI ACCTGC 1 cut(s) 536
Cac8I GCNNGC 2 cut(s) 60, 64
CaiI CAGNNNCTG 2 cut(s) 422, 551
CfoI GCGC 4 cut(s) 119, 146, 322, 331
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 2 cut(s) 548, 984
CseI GACGC 4 cut(s) 7, 18, 58, 499
Csp6I GTAC 2 cut(s) 756, 872
CviAII CATG 2 cut(s) 642, 776
CviQI GTAC 2 cut(s) 756, 872
DdeI CTNAG 3 cut(s) 279, 299, 606
DpnI GATC 4 cut(s) 132, 407, 785, 858
DpnII GATC 4 cut(s) 130, 405, 783, 856
EaeI YGGCCR 1 cut(s) 401
EagI CGGCCG 1 cut(s) 401
Eam1104I CTCTTC 2 cut(s) 299, 517
EarI CTCTTC 2 cut(s) 299, 517
Ecl136II GAGCTC 1 cut(s) 103
EclXI CGGCCG 1 cut(s) 401
Eco24I GRGCYC 1 cut(s) 105
Eco32I GATATC 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 548
Eco52I CGGCCG 1 cut(s) 401
Eco53kI GAGCTC 1 cut(s) 103
Eco88I CYCGRG 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 103
EcoO109I RGGNCCY 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 594
EcoRV GATATC 1 cut(s) 217
EcoT38I GRGCYC 1 cut(s) 105
FaeI CATG 2 cut(s) 645, 779
FatI CATG 2 cut(s) 641, 775
FauNDI CATATG 1 cut(s) 910
FbaI TGATCA 1 cut(s) 783
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 8 cut(s) 7, 10, 13, 16, 56, 67, 110, 417
FriOI GRGCYC 1 cut(s) 105
Fsp4HI GCNGC 8 cut(s) 7, 10, 13, 16, 56, 67, 110, 417
FspBI CTAG 2 cut(s) 384, 765
GlaI GCGC 4 cut(s) 118, 145, 321, 330
GluI GCNGC 8 cut(s) 7, 10, 13, 16, 56, 67, 110, 417
GsaI CCCAGC 1 cut(s) 497
GsuI CTGGAG 1 cut(s) 160
HaeII RGCGCY 2 cut(s) 120, 147
HaeIII GGCC 4 cut(s) 62, 263, 403, 986
HapII CCGG 1 cut(s) 63
HgaI GACGC 4 cut(s) 7, 18, 58, 499
HhaI GCGC 4 cut(s) 119, 146, 322, 331
Hin1I GRCGYC 2 cut(s) 29, 69
Hin1II CATG 2 cut(s) 645, 779
Hin6I GCGC 4 cut(s) 117, 144, 320, 329
HinP1I GCGC 4 cut(s) 117, 144, 320, 329
HincII GTYRAC 2 cut(s) 82, 1014
HindII GTYRAC 2 cut(s) 82, 1014
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 5 cut(s) 290, 525, 669, 689, 719
HpaII CCGG 1 cut(s) 63
HphI GGTGA 4 cut(s) 161, 482, 491, 698
Hpy166II GTNNAC 3 cut(s) 82, 916, 1014
Hpy188I TCNGA 2 cut(s) 460, 892
Hpy188III TCNNGA 4 cut(s) 139, 191, 200, 551
Hpy8I GTNNAC 3 cut(s) 82, 916, 1014
Hpy99I CGWCG 8 cut(s) 23, 34, 37, 74, 80, 83, 86, 402
HpyAV CCTTC 1 cut(s) 1010
HpyCH4III ACNGT 5 cut(s) 86, 184, 467, 755, 1032
HpyCH4IV ACGT 1 cut(s) 397
HpyCH4V TGCA 4 cut(s) 416, 563, 641, 960
HpyF10VI GCNNNNNNNGC 8 cut(s) 12, 15, 109, 150, 326, 328, 446, 713
HpyF3I CTNAG 3 cut(s) 279, 299, 606
HpySE526I ACGT 1 cut(s) 397
Hsp92I GRCGYC 2 cut(s) 29, 69
Hsp92II CATG 2 cut(s) 645, 779
HspAI GCGC 4 cut(s) 117, 144, 320, 329
KroI GCCGGC 1 cut(s) 62
KroNI GCCGGC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 783
Kzo9I GATC 4 cut(s) 130, 405, 783, 856
LmnI GCTCC 3 cut(s) 141, 429, 497
Lsp1109I GCAGC 4 cut(s) 18, 24, 27, 428
LweI GCATC 3 cut(s) 370, 428, 841
MaeI CTAG 2 cut(s) 384, 765
MaeII ACGT 1 cut(s) 397
MalI GATC 4 cut(s) 132, 407, 785, 858
MbiI CCGCTC 1 cut(s) 109
MboI GATC 4 cut(s) 130, 405, 783, 856
MboII GAAGA 6 cut(s) 316, 534, 629, 632, 635, 908
MhlI GDGCHC 1 cut(s) 105
MluCI AATT 1 cut(s) 792
MlyI GAGTC 2 cut(s) 534, 678
MroNI GCCGGC 1 cut(s) 62
MroXI GAANNNNTTC 1 cut(s) 573
MseI TTAA 2 cut(s) 750, 795
MspI CCGG 1 cut(s) 63
MspR9I CCNGG 1 cut(s) 596
MvaI CCWGG 1 cut(s) 596
MvnI CGCG 2 cut(s) 322, 331
MwoI GCNNNNNNNGC 8 cut(s) 12, 15, 109, 150, 326, 328, 446, 713
NaeI GCCGGC 1 cut(s) 64
NdeI CATATG 1 cut(s) 910
NdeII GATC 4 cut(s) 130, 405, 783, 856
NgoMIV GCCGGC 1 cut(s) 62
NlaIII CATG 2 cut(s) 645, 779
PaeR7I CTCGAG 1 cut(s) 104
PcsI WCGNNNNNNNCGW 2 cut(s) 32, 78
PdiI GCCGGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 573
PfeI GAWTC 3 cut(s) 290, 689, 719
PflMI CCANNNNNTGG 1 cut(s) 836
PkrI GCNGC 8 cut(s) 8, 11, 14, 17, 57, 68, 111, 418
PleI GAGTC 2 cut(s) 533, 677
PpsI GAGTC 2 cut(s) 533, 677
PpuMI RGGWCCY 1 cut(s) 548
PshAI GACNNNNGTC 1 cut(s) 87
Psp124BI GAGCTC 1 cut(s) 105
Psp5II RGGWCCY 1 cut(s) 548
Psp6I CCWGG 1 cut(s) 594
PspFI CCCAGC 1 cut(s) 493
PspGI CCWGG 1 cut(s) 594
PspPI GGNCC 2 cut(s) 548, 984
PspPPI RGGWCCY 1 cut(s) 548
PspXI VCTCGAGB 1 cut(s) 104
PstNI CAGNNNCTG 2 cut(s) 422, 551
RsaI GTAC 2 cut(s) 757, 873
RsaNI GTAC 2 cut(s) 756, 872
SacI GAGCTC 1 cut(s) 105
SalI GTCGAC 1 cut(s) 80
SaqAI TTAA 2 cut(s) 750, 795
SatI GCNGC 8 cut(s) 7, 10, 13, 16, 56, 67, 110, 417
Sau3AI GATC 4 cut(s) 130, 405, 783, 856
Sau96I GGNCC 2 cut(s) 548, 984
SchI GAGTC 2 cut(s) 534, 678
ScrFI CCNGG 1 cut(s) 596
SduI GDGCHC 1 cut(s) 105
SfaNI GCATC 3 cut(s) 370, 428, 841
Sfr274I CTCGAG 1 cut(s) 104
SgrDI CGTCGACG 1 cut(s) 80
SinI GGWCC 1 cut(s) 548
SlaI CTCGAG 1 cut(s) 104
SmlI CTYRAG 1 cut(s) 104
SmoI CTYRAG 1 cut(s) 104
Sse9I AATT 1 cut(s) 792
SsiI CCGC 4 cut(s) 10, 55, 66, 109
SspMI CTAG 2 cut(s) 384, 765
SstI GAGCTC 1 cut(s) 105
StyD4I CCNGG 1 cut(s) 594
TaaI ACNGT 5 cut(s) 86, 184, 467, 755, 1032
TaiI ACGT 1 cut(s) 400
TaqI TCGA 5 cut(s) 21, 81, 105, 275, 859
TaqII GACCGA 2 cut(s) 113, 999
TasI AATT 1 cut(s) 792
TatI WGTACW 1 cut(s) 755
TauI GCSGC 4 cut(s) 12, 58, 69, 112
TfiI GAWTC 3 cut(s) 290, 689, 719
Tru1I TTAA 2 cut(s) 750, 795
Tru9I TTAA 2 cut(s) 750, 795
TscAI CASTG 1 cut(s) 472
TseI GCWGC 4 cut(s) 6, 12, 15, 416
TspDTI ATGAA 5 cut(s) 176, 503, 764, 897, 909
TspRI CASTG 1 cut(s) 472
Van91I CCANNNNNTGG 1 cut(s) 836
VpaK11BI GGWCC 1 cut(s) 548
XhoI CTCGAG 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 81
XmnI GAANNNNTTC 1 cut(s) 573
XspI CTAG 2 cut(s) 384, 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.