Rw6G027940

Homocysteine s-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010707
Physical Location & Seq
Forward (+)
51911132 .. 51913388
2257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G027940.1

Sequence Viewer

Length: 1047 bp
ATGGGCAGCCGCAGCAGCAGCAGCAGCAGCGTCAAAATGGCGTCGTCGTCAATGAGGGAGTTTCTCCGGCAGGCAGGCGGCGTCGCCGTCGTCGACGGTGGTCTGGCGACCGAGCTCGAGCGGCACGGCGCTGACCTCAACGATCCTCTCTGGAGCGCCAAGTGCCTCCTCACTTCCTCTCACCTCATTCGCGCCGTTCATCTTGATTATCTTGAAGCTGGTGCTGATATCATAATCACAGCTTCTTATCAGGCAACTATTCAAGGTTTTGAGGCCAAAGGGTTTTCGACTGAGGAGAGTGAATCATTGCTTAGGAAGAGTGTGGAGATTGCGCGTGAGGCGCGAGATATTTATTACGAAAGGTGTAGTGAGGGGTCGTGTAATGATAGTGATGGTAGAGTTCTCAAACGTCGGCCCATCATAGTTGCAGCATCTGTGGGGAGCTATGGGGCTTATTTGGCTGATGGGTCTGAGTACAGCGGTGATTATGGTGAGGCTATGACGCTGGGAGCATTAAAAGAGTTTCATCGTAGAAGAGTCCAAGTCCTAACTGAAGCAGATCCTGACCTACTTGCATTTGAAACTATTCCAAATAAGCTGGAAGCCCAGGCTTTTGCTGAGCTTTTGGAAGAAGAAGACATAAAGCTTCCTGCATGGTTTTGTTTCAACTCTAAAGATGGAATCAATGTTGTTAGTGGTGATTCTTTCCTTGAATGTGCCTCGGTTGCTGAATCCTGTGAGAAAGTTGTTGCTGTGGGGATTAACTGTACTCCTCCTAGATTTATTCATGGATTGATCACAACAATTAAAAAGGTGGCTACAAAACCAATAGTTATATACCCAAATAGTGGCGAAAGTTATGATGCTGATCGAAAGGAGTGGGTACAAAATACTGGGGTTTCAGATGAAGATTTTGTTTCATATGTAAACAAATGGTGTGAGGCAGGGGCTTCCCTTGTGGGAGGTTGTTGCAGAACAACTCCAAATACTATCAGGGCCATCTACAAAACCCTTCCCAATCGGTCAACATCTCCACCTGAACAGTAA

Protein Analysis

348

Amino Acids

37.87

Weight (kDa)

5.0

Isoelectric Point (pI)

41.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S-methyl_trans PF02574 30 - 337 1.6e-81 Homocysteine S-methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010707)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63250 AT3G63250 AT3G63250
fragaria_vesca FvH4_2g23130 FvH4_2g23130
malus_domestica MD03G1260400.v1.1 MD11G1280700.v1.1
prunus_persica Prupe.8G236600_v2.0.a1
pyrus_communis pycom03g20810 pycom11g24850
rosa_chinensis RchiOBHm_Chr6g0290381
rosa_laevigata RLG00000012224
rosa_multiflora Rmu_sc0025482.1_g000001
rosa_roxburghii Rroxscaffold_7G00177120
rosa_rugosa Rorug06G0212100 Rorug06G0212100 Rorug06G0212200
rosa_samantha Rh6DG322700
rosa_wichuraiana Rw6G027840 Rw6G027940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 848
AccBSI CCGCTC 1 cut(s) 121
AccI GTMKAC 1 cut(s) 93
AccII CGCG 3 cut(s) 192, 334, 343
AciI CCGC 4 cut(s) 10, 78, 121, 480
AclWI GGATC 2 cut(s) 137, 554
AcuI CTGAAG 1 cut(s) 573
AcyI GRCGYC 2 cut(s) 41, 81
AfaI GTAC 3 cut(s) 476, 769, 885
AfiI CCNNNNNNNGG 1 cut(s) 848
AgsI TTSAA 5 cut(s) 215, 263, 581, 667, 713
AjnI CCWGG 1 cut(s) 606
AluBI AGCT 7 cut(s) 115, 218, 242, 444, 598, 622, 646
AluI AGCT 7 cut(s) 115, 218, 242, 444, 598, 622, 646
Alw21I GWGCWC 1 cut(s) 117
AlwI GGATC 2 cut(s) 137, 554
AlwNI CAGNNNCTG 2 cut(s) 434, 563
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 3 cut(s) 273, 413, 996
ApeKI GCWGC 8 cut(s) 6, 12, 15, 18, 21, 24, 27, 428
Asp700I GAANNNNTTC 1 cut(s) 585
AspLEI GCGC 5 cut(s) 131, 158, 194, 334, 343
AspS9I GGNCC 2 cut(s) 414, 996
AsuHPI GGTGA 4 cut(s) 173, 494, 503, 710
AvaI CYCGRG 1 cut(s) 116
BanII GRGCYC 1 cut(s) 117
BbsI GAAGAC 1 cut(s) 642
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 8 cut(s) 18, 24, 27, 30, 33, 36, 39, 440
BccI CCATC 5 cut(s) 386, 425, 458, 671, 1007
BceAI ACGGC 3 cut(s) 71, 142, 179
BciT130I CCWGG 1 cut(s) 608
BclI TGATCA 1 cut(s) 795
BfaI CTAG 1 cut(s) 777
BfoI RGCGCY 2 cut(s) 132, 159
BlpI GCTNAGC 1 cut(s) 618
Bme1390I CCNGG 1 cut(s) 608
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 2 cut(s) 414, 996
BmrFI CCNGG 1 cut(s) 608
BmrI ACTGGG 1 cut(s) 903
BmsI GCATC 2 cut(s) 440, 853
BmuI ACTGGG 1 cut(s) 903
BoxI GACNNNNGTC 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 642
BpmI CTGGAG 1 cut(s) 172
Bpu10I CCTNAGC 1 cut(s) 311
Bpu1102I GCTNAGC 1 cut(s) 618
BsaBI GATNNNNATC 1 cut(s) 867
BsaHI GRCGYC 2 cut(s) 41, 81
BsaJI CCNNGG 2 cut(s) 606, 720
Bsc4I CCNNNNNNNGG 1 cut(s) 848
Bse1I ACTGG 1 cut(s) 898
Bse3DI GCAATG 1 cut(s) 305
Bse8I GATNNNNATC 1 cut(s) 867
BseBI CCWGG 1 cut(s) 608
BseDI CCNNGG 2 cut(s) 606, 720
BseJI GATNNNNATC 1 cut(s) 867
BseLI CCNNNNNNNGG 1 cut(s) 848
BseMI GCAATG 1 cut(s) 305
BseMII CTCAG 3 cut(s) 282, 462, 609
BseNI ACTGG 1 cut(s) 898
BseRI GAGGAG 3 cut(s) 158, 308, 762
BseXI GCAGC 8 cut(s) 18, 24, 27, 30, 33, 36, 39, 440
BseYI CCCAGC 1 cut(s) 505
Bsh1236I CGCG 3 cut(s) 192, 334, 343
Bsh1285I CGRYCG 1 cut(s) 111
BshFI GGCC 3 cut(s) 275, 415, 998
BsiEI CGRYCG 1 cut(s) 111
BsiHKAI GWGCWC 1 cut(s) 117
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 1 cut(s) 67
BslI CCNNNNNNNGG 1 cut(s) 848
BsnI GGCC 3 cut(s) 275, 415, 998
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 117
Bsp143I GATC 4 cut(s) 142, 559, 795, 868
Bsp1720I GCTNAGC 1 cut(s) 618
BspACI CCGC 4 cut(s) 10, 78, 121, 480
BspANI GGCC 3 cut(s) 275, 415, 998
BspCNI CTCAG 3 cut(s) 283, 463, 610
BspFNI CGCG 3 cut(s) 192, 334, 343
BspPI GGATC 2 cut(s) 137, 554
BsrBI CCGCTC 1 cut(s) 121
BsrDI GCAATG 1 cut(s) 305
BsrI ACTGG 1 cut(s) 898
BssECI CCNNGG 2 cut(s) 606, 720
BssMI GATC 4 cut(s) 142, 559, 795, 868
BssNI GRCGYC 2 cut(s) 41, 81
Bst2UI CCWGG 1 cut(s) 608
Bst4CI ACNGT 3 cut(s) 98, 767, 1044
Bst6I CTCTTC 2 cut(s) 311, 529
BstACI GRCGYC 2 cut(s) 41, 81
BstC8I GCNNGC 2 cut(s) 72, 76
BstDEI CTNAG 4 cut(s) 291, 311, 471, 618
BstFNI CGCG 3 cut(s) 192, 334, 343
BstH2I RGCGCY 2 cut(s) 132, 159
BstHHI GCGC 5 cut(s) 131, 158, 194, 334, 343
BstKTI GATC 4 cut(s) 145, 562, 798, 871
BstMBI GATC 4 cut(s) 142, 559, 795, 868
BstMCI CGRYCG 1 cut(s) 111
BstNI CCWGG 1 cut(s) 608
BstPAI GACNNNNGTC 1 cut(s) 99
BstSCI CCNGG 1 cut(s) 606
BstUI CGCG 3 cut(s) 192, 334, 343
BstV1I GCAGC 8 cut(s) 18, 24, 27, 30, 33, 36, 39, 440
BstV2I GAAGAC 1 cut(s) 642
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
BsuRI GGCC 3 cut(s) 275, 415, 998
Cac8I GCNNGC 2 cut(s) 72, 76
CaiI CAGNNNCTG 2 cut(s) 434, 563
CfoI GCGC 5 cut(s) 131, 158, 194, 334, 343
Cfr13I GGNCC 2 cut(s) 414, 996
CseI GACGC 4 cut(s) 19, 30, 70, 511
Csp6I GTAC 3 cut(s) 475, 768, 884
CviAII CATG 2 cut(s) 654, 788
CviQI GTAC 3 cut(s) 475, 768, 884
DdeI CTNAG 4 cut(s) 291, 311, 471, 618
DpnI GATC 4 cut(s) 144, 561, 797, 870
DpnII GATC 4 cut(s) 142, 559, 795, 868
Eam1104I CTCTTC 2 cut(s) 311, 529
EarI CTCTTC 2 cut(s) 311, 529
Ecl136II GAGCTC 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 117
Eco32I GATATC 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 573
Eco88I CYCGRG 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 606
EcoRV GATATC 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 117
FaeI CATG 2 cut(s) 657, 791
FatI CATG 2 cut(s) 653, 787
FauNDI CATATG 1 cut(s) 922
FbaI TGATCA 1 cut(s) 795
FblI GTMKAC 1 cut(s) 93
FriOI GRGCYC 1 cut(s) 117
FspBI CTAG 1 cut(s) 777
GlaI GCGC 5 cut(s) 130, 157, 193, 333, 342
GsaI CCCAGC 1 cut(s) 509
GsuI CTGGAG 1 cut(s) 172
HaeII RGCGCY 2 cut(s) 132, 159
HaeIII GGCC 3 cut(s) 275, 415, 998
HapII CCGG 1 cut(s) 67
HgaI GACGC 4 cut(s) 19, 30, 70, 511
HhaI GCGC 5 cut(s) 131, 158, 194, 334, 343
Hin1I GRCGYC 2 cut(s) 41, 81
Hin1II CATG 2 cut(s) 657, 791
Hin6I GCGC 5 cut(s) 129, 156, 192, 332, 341
HinP1I GCGC 5 cut(s) 129, 156, 192, 332, 341
HincII GTYRAC 2 cut(s) 94, 1026
HindII GTYRAC 2 cut(s) 94, 1026
HindIII AAGCTT 1 cut(s) 644
HinfI GANTC 5 cut(s) 302, 537, 681, 701, 731
HpaII CCGG 1 cut(s) 67
HphI GGTGA 4 cut(s) 173, 494, 503, 710
Hpy166II GTNNAC 3 cut(s) 94, 928, 1026
Hpy188I TCNGA 2 cut(s) 472, 904
Hpy188III TCNNGA 4 cut(s) 151, 203, 212, 563
Hpy8I GTNNAC 3 cut(s) 94, 928, 1026
Hpy99I CGWCG 7 cut(s) 46, 49, 86, 92, 95, 98, 414
HpyAV CCTTC 1 cut(s) 1022
HpyCH4III ACNGT 3 cut(s) 98, 767, 1044
HpyCH4IV ACGT 1 cut(s) 409
HpyCH4V TGCA 4 cut(s) 428, 575, 653, 972
HpyF3I CTNAG 4 cut(s) 291, 311, 471, 618
HpySE526I ACGT 1 cut(s) 409
Hsp92I GRCGYC 2 cut(s) 41, 81
Hsp92II CATG 2 cut(s) 657, 791
HspAI GCGC 5 cut(s) 129, 156, 192, 332, 341
Ksp22I TGATCA 1 cut(s) 795
Kzo9I GATC 4 cut(s) 142, 559, 795, 868
LmnI GCTCC 3 cut(s) 153, 441, 509
Lsp1109I GCAGC 8 cut(s) 18, 24, 27, 30, 33, 36, 39, 440
LweI GCATC 2 cut(s) 440, 853
MaeI CTAG 1 cut(s) 777
MaeII ACGT 1 cut(s) 409
MalI GATC 4 cut(s) 144, 561, 797, 870
MbiI CCGCTC 1 cut(s) 121
MboI GATC 4 cut(s) 142, 559, 795, 868
MboII GAAGA 6 cut(s) 328, 546, 641, 644, 647, 920
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 1 cut(s) 804
MlyI GAGTC 1 cut(s) 546
MroXI GAANNNNTTC 1 cut(s) 585
MseI TTAA 3 cut(s) 515, 762, 807
MspA1I CMGCKG 1 cut(s) 480
MspI CCGG 1 cut(s) 67
MspR9I CCNGG 1 cut(s) 608
MvaI CCWGG 1 cut(s) 608
MvnI CGCG 3 cut(s) 192, 334, 343
NdeI CATATG 1 cut(s) 922
NdeII GATC 4 cut(s) 142, 559, 795, 868
NlaIII CATG 2 cut(s) 657, 791
PaeR7I CTCGAG 1 cut(s) 116
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 585
PfeI GAWTC 4 cut(s) 302, 681, 701, 731
PflMI CCANNNNNTGG 1 cut(s) 848
PleI GAGTC 1 cut(s) 545
PpsI GAGTC 1 cut(s) 545
PshAI GACNNNNGTC 1 cut(s) 99
Psp124BI GAGCTC 1 cut(s) 117
Psp6I CCWGG 1 cut(s) 606
PspFI CCCAGC 1 cut(s) 505
PspGI CCWGG 1 cut(s) 606
PspPI GGNCC 2 cut(s) 414, 996
PspXI VCTCGAGB 1 cut(s) 116
PstNI CAGNNNCTG 2 cut(s) 434, 563
PsuI RGATCY 1 cut(s) 559
RsaI GTAC 3 cut(s) 476, 769, 885
RsaNI GTAC 3 cut(s) 475, 768, 884
SacI GAGCTC 1 cut(s) 117
SalI GTCGAC 1 cut(s) 92
SaqAI TTAA 3 cut(s) 515, 762, 807
Sau3AI GATC 4 cut(s) 142, 559, 795, 868
Sau96I GGNCC 2 cut(s) 414, 996
SchI GAGTC 1 cut(s) 546
ScrFI CCNGG 1 cut(s) 608
SduI GDGCHC 1 cut(s) 117
SfaNI GCATC 2 cut(s) 440, 853
Sfr274I CTCGAG 1 cut(s) 116
SgrDI CGTCGACG 1 cut(s) 92
SlaI CTCGAG 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 1 cut(s) 804
SsiI CCGC 4 cut(s) 10, 78, 121, 480
SspMI CTAG 1 cut(s) 777
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 1 cut(s) 606
TaaI ACNGT 3 cut(s) 98, 767, 1044
TaiI ACGT 1 cut(s) 412
TaqI TCGA 4 cut(s) 93, 117, 287, 871
TaqII GACCGA 2 cut(s) 125, 1011
TasI AATT 1 cut(s) 804
TatI WGTACW 2 cut(s) 474, 767
TauI GCSGC 3 cut(s) 12, 81, 124
TfiI GAWTC 4 cut(s) 302, 681, 701, 731
Tru1I TTAA 3 cut(s) 515, 762, 807
Tru9I TTAA 3 cut(s) 515, 762, 807
TseI GCWGC 8 cut(s) 6, 12, 15, 18, 21, 24, 27, 428
TspDTI ATGAA 5 cut(s) 188, 515, 776, 909, 921
Van91I CCANNNNNTGG 1 cut(s) 848
XhoI CTCGAG 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 93
XmnI GAANNNNTTC 1 cut(s) 585
XspI CTAG 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.