AT4G00305

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012904
Physical Location & Seq
Forward (+)
131422 .. 132018
597 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G00305.1

Sequence Viewer

Length: 381 bp
ATGGGCTTATCTTATAGCGGCGCCGGAGTTATTATCGCCGGAGTTATCTTGAATGATTTTATCAAATCAGTTCGTTCTTTTCTTGATATTCGTCGTTTGTTTGGTTCGGCATCTTCAGGGAGCACTATCATCGACAAGATTCCGATGGACGATATGCTTCCGGCAACAAAGTTCGAGGATATATCATCAAGGGTTAATCCGCCGGAGAGCTGCCGGATATGCCAAGATGAGTTCGACGGCGGTGACGAAGTCCGGTGTTTGAGGAATTGCGTACATGTTTATCACAAGACGTGTATTGACCGTTGGATCCAAGACGATAAGATGACGTGTCCTTTGTGTAGGACCCCGATCGTTCCTGATTTCTATTTTTTTCGTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

126

Amino Acids

14.26

Weight (kDa)

5.6

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 69 - 114 2.9e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 70 - 114 1.1e-11 Ring finger domain
zf-C3HC4_2 PF13923 71 - 113 1.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 71 - 99 3.1e-07 RING-like zinc finger
zf-C3HC4 PF00097 71 - 113 1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 71 - 111 1.4e-06 RING-type zinc-finger
zf-RING_5 PF14634 71 - 115 1e-05 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 20
AciI CCGC 3 cut(s) 18, 200, 240
AclWI GGATC 2 cut(s) 301, 314
AcuI CTGAAG 1 cut(s) 99
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 1 cut(s) 273
AflIII ACRYGT 3 cut(s) 274, 290, 326
AgsI TTSAA 1 cut(s) 52
AjiI CACGTC 2 cut(s) 291, 327
AluBI AGCT 1 cut(s) 210
AluI AGCT 1 cut(s) 210
Alw21I GWGCWC 1 cut(s) 125
AlwI GGATC 2 cut(s) 301, 314
ApeKI GCWGC 1 cut(s) 210
ArsI GACNNNNNNTTYG 2 cut(s) 316, 348
AspLEI GCGC 1 cut(s) 23
AspS9I GGNCC 1 cut(s) 342
AsuHPI GGTGA 1 cut(s) 254
AvaII GGWCC 1 cut(s) 342
BamHI GGATCC 1 cut(s) 306
BanI GGYRCC 1 cut(s) 20
Bbv12I GWGCWC 1 cut(s) 125
BbvI GCAGC 1 cut(s) 197
BccI CCATC 1 cut(s) 139
BceAI ACGGC 1 cut(s) 253
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BfoI RGCGCY 1 cut(s) 24
BisI GCNGC 2 cut(s) 19, 211
BlsI GCNGC 2 cut(s) 20, 212
Bme18I GGWCC 1 cut(s) 342
BmgBI CACGTC 2 cut(s) 291, 327
BmgT120I GGNCC 1 cut(s) 342
BmiI GGNNCC 3 cut(s) 22, 308, 344
BmsI GCATC 1 cut(s) 119
BsaHI GRCGYC 1 cut(s) 21
BsaWI WCCGGW 1 cut(s) 252
BseXI GCAGC 1 cut(s) 197
Bsh1285I CGRYCG 1 cut(s) 351
BshNI GGYRCC 1 cut(s) 20
BsiEI CGRYCG 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 125
BsiSI CCGG 6 cut(s) 24, 39, 161, 203, 214, 253
Bsp1286I GDGCHC 1 cut(s) 125
Bsp143I GATC 2 cut(s) 306, 348
BspACI CCGC 3 cut(s) 18, 200, 240
BspLI GGNNCC 3 cut(s) 22, 308, 344
BspPI GGATC 2 cut(s) 301, 314
BspT107I GGYRCC 1 cut(s) 20
BssMI GATC 2 cut(s) 306, 348
BssNI GRCGYC 1 cut(s) 21
Bst4CI ACNGT 1 cut(s) 302
BstACI GRCGYC 1 cut(s) 21
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 1 cut(s) 23
BstKTI GATC 2 cut(s) 309, 351
BstMBI GATC 2 cut(s) 306, 348
BstMCI CGRYCG 1 cut(s) 351
BstMWI GCNNNNNNNGC 1 cut(s) 219
BstNSI RCATGY 1 cut(s) 278
BstV1I GCAGC 1 cut(s) 197
BstX2I RGATCY 1 cut(s) 306
BstYI RGATCY 1 cut(s) 306
BtrI CACGTC 2 cut(s) 291, 327
CfoI GCGC 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 342
Csp6I GTAC 1 cut(s) 272
CviAII CATG 1 cut(s) 275
CviJI RGCY 2 cut(s) 6, 210
CviKI_1 RGCY 2 cut(s) 6, 210
CviQI GTAC 1 cut(s) 272
DinI GGCGCC 1 cut(s) 22
DpnI GATC 2 cut(s) 308, 350
DpnII GATC 2 cut(s) 306, 348
EciI GGCGGA 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 342
Eco57I CTGAAG 1 cut(s) 99
EcoO109I RGGNCCY 1 cut(s) 342
EgeI GGCGCC 1 cut(s) 22
EheI GGCGCC 1 cut(s) 22
FaeI CATG 1 cut(s) 278
FaiI YATR 5 cut(s) 15, 155, 182, 220, 276
FatI CATG 1 cut(s) 274
Fnu4HI GCNGC 2 cut(s) 19, 211
Fsp4HI GCNGC 2 cut(s) 19, 211
GlaI GCGC 1 cut(s) 22
GluI GCNGC 2 cut(s) 19, 211
HaeII RGCGCY 1 cut(s) 24
HapII CCGG 6 cut(s) 24, 39, 161, 203, 214, 253
HhaI GCGC 1 cut(s) 23
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 1 cut(s) 278
Hin6I GCGC 1 cut(s) 21
HinP1I GCGC 1 cut(s) 21
HinfI GANTC 1 cut(s) 139
HpaII CCGG 6 cut(s) 24, 39, 161, 203, 214, 253
HphI GGTGA 1 cut(s) 254
Hpy188I TCNGA 1 cut(s) 144
Hpy188III TCNNGA 3 cut(s) 49, 83, 356
Hpy99I CGWCG 2 cut(s) 96, 239
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4IV ACGT 2 cut(s) 290, 326
HpyF10VI GCNNNNNNNGC 1 cut(s) 219
HpySE526I ACGT 2 cut(s) 290, 326
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 1 cut(s) 278
HspAI GCGC 1 cut(s) 21
KasI GGCGCC 1 cut(s) 20
Kzo9I GATC 2 cut(s) 306, 348
LmnI GCTCC 1 cut(s) 120
LpnPI CCDG 8 cut(s) 37, 52, 102, 174, 216, 227, 266, 369
Lsp1109I GCAGC 1 cut(s) 197
LweI GCATC 1 cut(s) 119
MaeII ACGT 2 cut(s) 290, 326
MaeIII GTNAC 1 cut(s) 242
MalI GATC 2 cut(s) 308, 350
MboI GATC 2 cut(s) 306, 348
MboII GAAGA 1 cut(s) 105
MflI RGATCY 1 cut(s) 306
MhlI GDGCHC 1 cut(s) 125
MluCI AATT 1 cut(s) 265
Mly113I GGCGCC 1 cut(s) 21
MmeI TCCRAC 1 cut(s) 284
MnlI CCTC 2 cut(s) 169, 255
MseI TTAA 1 cut(s) 195
MspI CCGG 6 cut(s) 24, 39, 161, 203, 214, 253
MwoI GCNNNNNNNGC 1 cut(s) 219
NarI GGCGCC 1 cut(s) 21
NdeII GATC 2 cut(s) 306, 348
NlaIII CATG 1 cut(s) 278
NlaIV GGNNCC 3 cut(s) 22, 308, 344
NmuCI GTSAC 1 cut(s) 242
NspI RCATGY 1 cut(s) 278
PciI ACATGT 1 cut(s) 274
PcsI WCGNNNNNNNCGW 1 cut(s) 243
PfeI GAWTC 1 cut(s) 139
PflFI GACNNNGTC 1 cut(s) 248
PkrI GCNGC 2 cut(s) 20, 212
Ple19I CGATCG 1 cut(s) 351
PluTI GGCGCC 1 cut(s) 24
PpuMI RGGWCCY 1 cut(s) 342
PscI ACATGT 1 cut(s) 274
Psp5II RGGWCCY 1 cut(s) 342
PspN4I GGNNCC 3 cut(s) 22, 308, 344
PspPI GGNCC 1 cut(s) 342
PspPPI RGGWCCY 1 cut(s) 342
PsuI RGATCY 1 cut(s) 306
PsyI GACNNNGTC 1 cut(s) 248
PvuI CGATCG 1 cut(s) 351
RsaI GTAC 1 cut(s) 273
RsaNI GTAC 1 cut(s) 272
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 2 cut(s) 19, 211
Sau3AI GATC 2 cut(s) 306, 348
Sau96I GGNCC 1 cut(s) 342
SduI GDGCHC 1 cut(s) 125
SetI ASST 3 cut(s) 212, 293, 329
SfaNI GCATC 1 cut(s) 119
SfoI GGCGCC 1 cut(s) 22
SinI GGWCC 1 cut(s) 342
Sse9I AATT 1 cut(s) 265
SsiI CCGC 3 cut(s) 18, 200, 240
SspDI GGCGCC 1 cut(s) 20
TaaI ACNGT 1 cut(s) 302
TaiI ACGT 2 cut(s) 293, 329
TaqI TCGA 3 cut(s) 132, 174, 234
TasI AATT 1 cut(s) 265
TauI GCSGC 1 cut(s) 21
TfiI GAWTC 1 cut(s) 139
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TseFI GTSAC 1 cut(s) 242
TseI GCWGC 1 cut(s) 210
Tsp45I GTSAC 1 cut(s) 242
Tth111I GACNNNGTC 1 cut(s) 248
VpaK11BI GGWCC 1 cut(s) 342
XceI RCATGY 1 cut(s) 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.