Rroxscaffold_3G00236340

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012904
Physical Location & Seq
Reverse (-)
23531751 .. 23534114
2364 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00236340.1

Sequence Viewer

Length: 366 bp
ATGCCCTTCTGCATCAGTGCTGAAATCGACATCTTCTTCACTGATTATGTTGGTCAAAGCGGTGACCTTGATTCGAGTAGTACTGACTTGGATGCTCTGTTTGAGAGAATTTCATCTACTGCTCAAGGTTCAAGTGTTCTGGACAAATTCATGGCTAATAGCTTTGTGGCCGAGAAGGATTCGGATAAGTGCACTATTTGTATGGAGGACTTTGATGGGACTAGTACTCTCATTCGAACCCCGTGCGCTCATGCCTTTCACAAATCATGTGTTGTTCGGTGGTTGATCATGAACAAGAACACATGTCCCTTGTGCCGCAGTCAACTCCTCGATTGTCTCCATCCAACCACCATATCTCTTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

121

Amino Acids

13.38

Weight (kDa)

4.63

Isoelectric Point (pI)

53.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 61 - 109 3.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 62 - 106 9.2e-15 Ring finger domain
zf-rbx1 PF12678 62 - 106 9.9e-09 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 63 - 105 1.4e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 63 - 106 1.5e-07 zinc-RING finger domain
zf-RING_11 PF17123 64 - 91 5.4e-09 RING-like zinc finger
zf-C3HC4 PF00097 64 - 105 1.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 64 - 103 7.3e-08 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 60, 316
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 2 cut(s) 108, 146
AfaI GTAC 2 cut(s) 82, 226
AflIII ACRYGT 1 cut(s) 302
AgsI TTSAA 1 cut(s) 132
AhlI ACTAGT 1 cut(s) 221
AluBI AGCT 1 cut(s) 162
AluI AGCT 1 cut(s) 162
Alw21I GWGCWC 1 cut(s) 194
Alw26I GTCTC 1 cut(s) 341
Alw44I GTGCAC 1 cut(s) 190
AoxI GGCC 1 cut(s) 168
ApaLI GTGCAC 1 cut(s) 190
ApoI RAATTY 2 cut(s) 108, 146
AspLEI GCGC 1 cut(s) 248
AsuHPI GGTGA 1 cut(s) 74
AsuII TTCGAA 1 cut(s) 235
BaeGI GKGCMC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 194
BccI CCATC 2 cut(s) 209, 348
BcgI CGANNNNNNTGC 2 cut(s) 225, 259
BclI TGATCA 1 cut(s) 285
BcoDI GTCTC 1 cut(s) 341
BcuI ACTAGT 1 cut(s) 221
BfaI CTAG 1 cut(s) 222
BisI GCNGC 1 cut(s) 316
BlsI GCNGC 1 cut(s) 317
BmcAI AGTACT 2 cut(s) 82, 226
BmsI GCATC 2 cut(s) 21, 82
Bpu14I TTCGAA 1 cut(s) 235
BpuEI CTTGAG 1 cut(s) 108
BseGI GGATG 2 cut(s) 97, 340
BseRI GAGGAG 1 cut(s) 317
BseSI GKGCMC 1 cut(s) 194
BshFI GGCC 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 194
BslFI GGGAC 2 cut(s) 232, 291
BsmAI GTCTC 1 cut(s) 341
BsmFI GGGAC 2 cut(s) 232, 291
BsnI GGCC 1 cut(s) 170
Bsp119I TTCGAA 1 cut(s) 235
Bsp1286I GDGCHC 1 cut(s) 194
Bsp143I GATC 1 cut(s) 285
BspACI CCGC 2 cut(s) 60, 316
BspANI GGCC 1 cut(s) 170
BspHI TCATGA 1 cut(s) 288
BspT104I TTCGAA 1 cut(s) 235
BssMI GATC 1 cut(s) 285
BstBI TTCGAA 1 cut(s) 235
BstEII GGTNACC 1 cut(s) 62
BstF5I GGATG 2 cut(s) 97, 340
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 1 cut(s) 288
BstMAI GTCTC 1 cut(s) 341
BstMBI GATC 1 cut(s) 285
BstNSI RCATGY 1 cut(s) 306
BstPI GGTNACC 1 cut(s) 62
BstSLI GKGCMC 1 cut(s) 194
BsuRI GGCC 1 cut(s) 170
BtsCI GGATG 2 cut(s) 97, 340
BtsIMutI CAGTG 2 cut(s) 22, 39
CciI TCATGA 1 cut(s) 288
CfoI GCGC 1 cut(s) 248
Csp6I GTAC 2 cut(s) 81, 225
CviAII CATG 5 cut(s) 151, 251, 267, 289, 303
CviJI RGCY 3 cut(s) 155, 162, 170
CviKI_1 RGCY 3 cut(s) 155, 162, 170
CviQI GTAC 2 cut(s) 81, 225
DpnI GATC 1 cut(s) 287
DpnII GATC 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 168
Eco91I GGTNACC 1 cut(s) 62
EcoO65I GGTNACC 1 cut(s) 62
FaeI CATG 5 cut(s) 154, 254, 270, 292, 306
FaiI YATR 8 cut(s) 48, 152, 203, 252, 268, 290, 304, 353
FaqI GGGAC 2 cut(s) 232, 291
FatI CATG 5 cut(s) 150, 250, 266, 288, 302
FbaI TGATCA 1 cut(s) 285
Fnu4HI GCNGC 1 cut(s) 316
FokI GGATG 2 cut(s) 104, 327
Fsp4HI GCNGC 1 cut(s) 316
FspBI CTAG 1 cut(s) 222
GlaI GCGC 1 cut(s) 247
GluI GCNGC 1 cut(s) 316
HaeIII GGCC 1 cut(s) 170
HhaI GCGC 1 cut(s) 248
Hin1II CATG 5 cut(s) 154, 254, 270, 292, 306
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HincII GTYRAC 1 cut(s) 323
HindII GTYRAC 1 cut(s) 323
HinfI GANTC 2 cut(s) 71, 179
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 2 cut(s) 192, 323
Hpy188I TCNGA 1 cut(s) 184
Hpy188III TCNNGA 2 cut(s) 140, 289
Hpy8I GTNNAC 2 cut(s) 192, 323
HpyAV CCTTC 2 cut(s) 16, 169
HpyCH4V TGCA 2 cut(s) 12, 192
Hsp92II CATG 5 cut(s) 154, 254, 270, 292, 306
HspAI GCGC 1 cut(s) 246
Ksp22I TGATCA 1 cut(s) 285
Kzo9I GATC 1 cut(s) 285
LpnPI CCDG 1 cut(s) 125
LweI GCATC 2 cut(s) 21, 82
MaeI CTAG 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 62
MalI GATC 1 cut(s) 287
MboI GATC 1 cut(s) 285
MboII GAAGA 2 cut(s) 25, 28
MhlI GDGCHC 1 cut(s) 194
MluCI AATT 2 cut(s) 108, 146
MnlI CCTC 2 cut(s) 199, 338
NdeII GATC 1 cut(s) 285
NlaIII CATG 5 cut(s) 154, 254, 270, 292, 306
NmeAIII GCCGAG 1 cut(s) 196
NmuCI GTSAC 1 cut(s) 62
NspI RCATGY 1 cut(s) 306
NspV TTCGAA 1 cut(s) 235
PagI TCATGA 1 cut(s) 288
PciI ACATGT 1 cut(s) 302
PfeI GAWTC 2 cut(s) 71, 179
PkrI GCNGC 1 cut(s) 317
PscI ACATGT 1 cut(s) 302
PspEI GGTNACC 1 cut(s) 62
RsaI GTAC 2 cut(s) 82, 226
RsaNI GTAC 2 cut(s) 81, 225
SatI GCNGC 1 cut(s) 316
Sau3AI GATC 1 cut(s) 285
ScaI AGTACT 2 cut(s) 82, 226
SduI GDGCHC 1 cut(s) 194
SetI ASST 3 cut(s) 69, 130, 164
SfaNI GCATC 2 cut(s) 21, 82
SfuI TTCGAA 1 cut(s) 235
SmlI CTYRAG 1 cut(s) 123
SmoI CTYRAG 1 cut(s) 123
SpeI ACTAGT 1 cut(s) 221
Sse9I AATT 2 cut(s) 108, 146
SsiI CCGC 2 cut(s) 60, 316
SspMI CTAG 1 cut(s) 222
TaqI TCGA 4 cut(s) 27, 74, 235, 330
TasI AATT 2 cut(s) 108, 146
TatI WGTACW 2 cut(s) 80, 224
TauI GCSGC 1 cut(s) 318
TfiI GAWTC 2 cut(s) 71, 179
TscAI CASTG 2 cut(s) 22, 46
TseFI GTSAC 1 cut(s) 62
Tsp45I GTSAC 1 cut(s) 62
TspDTI ATGAA 3 cut(s) 102, 139, 305
TspRI CASTG 2 cut(s) 22, 46
VneI GTGCAC 1 cut(s) 190
XapI RAATTY 2 cut(s) 108, 146
XceI RCATGY 1 cut(s) 306
XspI CTAG 1 cut(s) 222
ZrmI AGTACT 2 cut(s) 82, 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.