Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0013163

Physical Location & Seq

Genomic Coordinates Reverse (-)

255320 .. 256610

Gene Structure 1291 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G00600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 933 bp

ATGTTTACTGATTGCTCCTCCTCCACAACGTCGCGTCTCATCCATTTATACAACCGCAATGGTGTTTTCCTCCCCCGCCCGAGCGTCAGCCAATTCAGCTTACGGACAACTGCGTCTACTTGGCGCTGCACTCTCTCCATTCGCTCCTCTTCTTCTCCATCTGCGATTGTAATTGATGGAAAGGCTGAGGCCAAAAAGATTAGAGATGATATCAAAATCGAAGTTTCAAGAATGAAGGAATCAATCGGTGTGGTTCCTGCTGAAGATTCATCAGAAGAAGAGGTGTTGAAATATGTATCAGGATTCAATGATGATCCTTCTGTTCACGGAGTCCTTGTTCAGTTGCCTCTACCATCGCATATGGATGAACAGAACATATTGAATGCGGTTAGTATAGAGAAGGACGTTGATGGATTTCACCCGCTAAATATTGGACGGCTTGCCATGCGTGGTAGAGAACCTTTATTCGTTCCATGTACTCCAAAAGGATGCATCGAGTTGTTGCACAGATACAACATTGAATTCAAAGGCAAAAGAGCAGTTGTTATTGGAAGGAGTAACATTGTTGGTATGCCAGCTGCTCTGTTGCTGCAGAAGGAGGATGCAACCGTTAGCATTATCCATTCAAGAACCATGAACCCTGAAGAACTCACAAGACAAGCTGATATCCTAATCTCAGCTGTTGGGAAACCAAACATGGTCAGAGGAAGCTGGATAAAACCGGGTGCAGTCCTCATCGATGTTGGGATTAAACCTGTTGAGGATCCAAGTGCTGCCGGTGGAGAGCGGTTGGTTGGAGACATTTGCTATGTGGAGGCTAGCAAAATTGCATCAGCCATCACACCTGTTCCTGGTGATGTAGGACCAATGACCATAGCCATGCTTCTATCCAACACTTTAACATCAGCCAAGAGAATTCACAACTTCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004477 GO:0004488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006730 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0019238 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

310

Amino Acids

33.7

Weight (kDa)

7.68

Isoelectric Point (pI)

50.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THF_DHG_CYH	PF00763	87 - 137	7.5e-17	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C	PF02882	158 - 305	9.1e-59	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013163)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00600 AT4G00620
fragaria_vesca	FvH4_7g13650 FvH4_7g13650
malus_domestica	MD07G1141500.v1.1 MD08G1243500.v1.1
prunus_persica	Prupe.2G162600_v2.0.a1
pyrus_communis	pycom07g14040 pycom08g21120
rosa_chinensis	RchiOBHm_Chr1g0353731
rosa_laevigata	RLG00000028263
rosa_roxburghii	Rroxscaffold_4G00301710
rosa_rugosa	Rorug01G0237200
rosa_samantha	Rh1AG249400 Rh1BG219500 Rh1CG232100 Rh1DG246500
rosa_wichuraiana	Rw1G021760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	112
AccBSI	CCGCTC	1 cut(s)	787
AccI	GTMKAC	1 cut(s)	116
AccII	CGCG	1 cut(s)	34
AciI	CCGC	5 cut(s)	55, 76, 386, 422, 787
AclWI	GGATC	3 cut(s)	308, 758, 771
AcsI	RAATTY	2 cut(s)	521, 915
AcuI	CTGAAG	2 cut(s)	282, 663
AfaI	GTAC	1 cut(s)	478
AfiI	CCNNNNNNNGG	1 cut(s)	851
AgsI	TTSAA	7 cut(s)	228, 289, 307, 382, 521, 526, 627
AjnI	CCWGG	1 cut(s)	850
AloI	GAACNNNNNNTCC	2 cut(s)	321, 353
AluBI	AGCT	5 cut(s)	99, 578, 662, 680, 711
AluI	AGCT	5 cut(s)	99, 578, 662, 680, 711
Alw26I	GTCTC	2 cut(s)	41, 792
AlwI	GGATC	3 cut(s)	308, 758, 771
Ama87I	CYCGRG	1 cut(s)	79
AoxI	GGCC	1 cut(s)	189
ApeKI	GCWGC	4 cut(s)	126, 578, 589, 773
ApoI	RAATTY	2 cut(s)	521, 915
AspLEI	GCGC	1 cut(s)	126
AspS9I	GGNCC	1 cut(s)	863
AsuC2I	CCSGG	1 cut(s)	723
AsuHPI	GGTGA	2 cut(s)	410, 866
AsuNHI	GCTAGC	1 cut(s)	818
AvaI	CYCGRG	1 cut(s)	79
AvaII	GGWCC	1 cut(s)	863
BamHI	GGATCC	1 cut(s)	763
BbvCI	CCTCAGC	1 cut(s)	186
BbvI	GCAGC	4 cut(s)	113, 565, 576, 760
BccI	CCATC	5 cut(s)	166, 170, 361, 404, 845
BceAI	ACGGC	1 cut(s)	452
BciT130I	CCWGG	1 cut(s)	852
BcnI	CCSGG	1 cut(s)	723
BcoDI	GTCTC	2 cut(s)	41, 792
BfaI	CTAG	1 cut(s)	819
BfmI	CTRYAG	1 cut(s)	590
BfoI	RGCGCY	1 cut(s)	127
BisI	GCNGC	4 cut(s)	127, 579, 590, 774
BlsI	GCNGC	4 cut(s)	128, 580, 591, 775
Bme1390I	CCNGG	2 cut(s)	723, 852
Bme18I	GGWCC	1 cut(s)	863
BmeT110I	CYCGRG	1 cut(s)	79
BmgT120I	GGNCC	1 cut(s)	863
BmiI	GGNNCC	2 cut(s)	255, 765
BmrFI	CCNGG	2 cut(s)	723, 852
BmsI	GCATC	4 cut(s)	479, 501, 592, 839
BmtI	GCTAGC	1 cut(s)	822
Bpu10I	CCTNAGC	1 cut(s)	186
BpuMI	CCSGG	1 cut(s)	723
Bsa29I	ATCGAT	1 cut(s)	738
BsaXI	ACNNNNNCTCC	2 cut(s)	321, 351
Bsc4I	CCNNNNNNNGG	1 cut(s)	851
Bse118I	RCCGGY	1 cut(s)	776
Bse1I	ACTGG	1 cut(s)	928
Bse3DI	GCAATG	1 cut(s)	64
BseBI	CCWGG	1 cut(s)	852
BseCI	ATCGAT	1 cut(s)	738
BseGI	GGATG	4 cut(s)	39, 370, 494, 607
BseLI	CCNNNNNNNGG	1 cut(s)	851
BseMI	GCAATG	1 cut(s)	64
BseMII	CTCAG	2 cut(s)	177, 690
BseNI	ACTGG	1 cut(s)	928
BseRI	GAGGAG	3 cut(s)	7, 10, 136
BseXI	GCAGC	4 cut(s)	113, 565, 576, 760
BsgI	GTGCAG	2 cut(s)	112, 747
Bsh1236I	CGCG	1 cut(s)	34
BshFI	GGCC	1 cut(s)	191
BshVI	ATCGAT	1 cut(s)	738
BsiHKCI	CYCGRG	1 cut(s)	79
BsiSI	CCGG	2 cut(s)	722, 777
BslI	CCNNNNNNNGG	1 cut(s)	851
BsmAI	GTCTC	2 cut(s)	41, 792
BsmBI	CGTCTC	1 cut(s)	41
BsmI	GAATGC	1 cut(s)	388
BsnI	GGCC	1 cut(s)	191
BsoBI	CYCGRG	1 cut(s)	79
Bsp143I	GATC	2 cut(s)	313, 763
BspACI	CCGC	5 cut(s)	55, 76, 386, 422, 787
BspANI	GGCC	1 cut(s)	191
BspCNI	CTCAG	2 cut(s)	178, 689
BspDI	ATCGAT	1 cut(s)	738
BspFNI	CGCG	1 cut(s)	34
BspLI	GGNNCC	2 cut(s)	255, 765
BspMAI	CTGCAG	1 cut(s)	594
BspOI	GCTAGC	1 cut(s)	822
BspPI	GGATC	3 cut(s)	308, 758, 771
BsrBI	CCGCTC	1 cut(s)	787
BsrDI	GCAATG	1 cut(s)	64
BsrFI	RCCGGY	1 cut(s)	776
BsrI	ACTGG	1 cut(s)	928
BssAI	RCCGGY	1 cut(s)	776
BssMI	GATC	2 cut(s)	313, 763
Bst2UI	CCWGG	1 cut(s)	852
Bst4CI	ACNGT	1 cut(s)	610
Bst6I	CTCTTC	2 cut(s)	154, 273
BstC8I	GCNNGC	3 cut(s)	441, 576, 820
BstDEI	CTNAG	2 cut(s)	186, 676
BstF5I	GGATG	4 cut(s)	39, 370, 494, 607
BstFNI	CGCG	1 cut(s)	34
BstH2I	RGCGCY	1 cut(s)	127
BstHHI	GCGC	1 cut(s)	126
BstKTI	GATC	2 cut(s)	316, 766
BstMAI	GTCTC	2 cut(s)	41, 792
BstMBI	GATC	2 cut(s)	313, 763
BstMWI	GCNNNNNNNGC	2 cut(s)	96, 445
BstNI	CCWGG	1 cut(s)	852
BstSCI	CCNGG	2 cut(s)	721, 850
BstSFI	CTRYAG	1 cut(s)	590
BstUI	CGCG	1 cut(s)	34
BstV1I	GCAGC	4 cut(s)	113, 565, 576, 760
BstX2I	RGATCY	1 cut(s)	763
BstYI	RGATCY	1 cut(s)	763
Bsu15I	ATCGAT	1 cut(s)	738
BsuRI	GGCC	1 cut(s)	191
BsuTUI	ATCGAT	1 cut(s)	738
BtgZI	GCGATG	1 cut(s)	339
BtsCI	GGATG	4 cut(s)	39, 370, 494, 607
Cac8I	GCNNGC	3 cut(s)	441, 576, 820
CfoI	GCGC	1 cut(s)	126
Cfr10I	RCCGGY	1 cut(s)	776
Cfr13I	GGNCC	1 cut(s)	863
ClaI	ATCGAT	1 cut(s)	738
CseI	GACGC	3 cut(s)	23, 73, 102
Csp6I	GTAC	1 cut(s)	477
CspCI	CAANNNNNGTGG	2 cut(s)	231, 266
CviAII	CATG	5 cut(s)	445, 474, 634, 697, 880
CviQI	GTAC	1 cut(s)	477
DdeI	CTNAG	2 cut(s)	186, 676
DpnI	GATC	2 cut(s)	315, 765
DpnII	GATC	2 cut(s)	313, 763
DrdI	GACNNNNNNGTC	1 cut(s)	112
DseDI	GACNNNNNNGTC	1 cut(s)	112
Eam1104I	CTCTTC	2 cut(s)	154, 273
EarI	CTCTTC	2 cut(s)	154, 273
Eco32I	GATATC	2 cut(s)	211, 667
Eco47I	GGWCC	1 cut(s)	863
Eco57I	CTGAAG	2 cut(s)	282, 663
Eco88I	CYCGRG	1 cut(s)	79
EcoRI	GAATTC	2 cut(s)	521, 915
EcoRII	CCWGG	1 cut(s)	850
EcoRV	GATATC	2 cut(s)	211, 667
EcoT22I	ATGCAT	1 cut(s)	494
Esp3I	CGTCTC	1 cut(s)	41
FaeI	CATG	5 cut(s)	448, 477, 637, 700, 883
FatI	CATG	5 cut(s)	444, 473, 633, 696, 879
FauI	CCCGC	2 cut(s)	83, 429
FauNDI	CATATG	1 cut(s)	360
FblI	GTMKAC	1 cut(s)	116
Fnu4HI	GCNGC	4 cut(s)	127, 579, 590, 774
FokI	GGATG	4 cut(s)	26, 377, 501, 614
Fsp4HI	GCNGC	4 cut(s)	127, 579, 590, 774
FspBI	CTAG	1 cut(s)	819
GlaI	GCGC	1 cut(s)	125
GluI	GCNGC	4 cut(s)	127, 579, 590, 774
HaeII	RGCGCY	1 cut(s)	127
HaeIII	GGCC	1 cut(s)	191
HapII	CCGG	2 cut(s)	722, 777
HgaI	GACGC	3 cut(s)	23, 73, 102
HhaI	GCGC	1 cut(s)	126
Hin1II	CATG	5 cut(s)	448, 477, 637, 700, 883
Hin6I	GCGC	1 cut(s)	124
HinP1I	GCGC	1 cut(s)	124
HinfI	GANTC	4 cut(s)	239, 266, 303, 330
HpaII	CCGG	2 cut(s)	722, 777
HphI	GGTGA	2 cut(s)	410, 866
Hpy166II	GTNNAC	3 cut(s)	6, 117, 325
Hpy188I	TCNGA	2 cut(s)	274, 704
Hpy188III	TCNNGA	3 cut(s)	228, 300, 627
Hpy8I	GTNNAC	3 cut(s)	6, 117, 325
Hpy99I	CGWCG	1 cut(s)	34
HpyAV	CCTTC	5 cut(s)	229, 327, 394, 546, 589
HpyCH4III	ACNGT	1 cut(s)	610
HpyCH4IV	ACGT	2 cut(s)	29, 405
HpyCH4V	TGCA	7 cut(s)	129, 492, 505, 592, 605, 728, 830
HpyF10VI	GCNNNNNNNGC	2 cut(s)	96, 445
HpyF3I	CTNAG	2 cut(s)	186, 676
HpySE526I	ACGT	2 cut(s)	29, 405
Hsp92II	CATG	5 cut(s)	448, 477, 637, 700, 883
HspAI	GCGC	1 cut(s)	124
Kzo9I	GATC	2 cut(s)	313, 763
LmnI	GCTCC	2 cut(s)	20, 149
Lsp1109I	GCAGC	4 cut(s)	113, 565, 576, 760
LweI	GCATC	4 cut(s)	479, 501, 592, 839
MaeI	CTAG	1 cut(s)	819
MaeII	ACGT	2 cut(s)	29, 405
MaeIII	GTNAC	1 cut(s)	557
MalI	GATC	2 cut(s)	315, 765
MbiI	CCGCTC	1 cut(s)	787
MboI	GATC	2 cut(s)	313, 763
MboII	GAAGA	6 cut(s)	141, 144, 275, 287, 290, 656
MflI	RGATCY	1 cut(s)	763
MluCI	AATT	5 cut(s)	92, 171, 521, 825, 915
MlyI	GAGTC	1 cut(s)	339
MmeI	TCCRAC	2 cut(s)	775, 915
Mph1103I	ATGCAT	1 cut(s)	494
MseI	TTAA	2 cut(s)	750, 899
MslI	CAYNNNNRTG	2 cut(s)	363, 878
MspA1I	CMGCKG	2 cut(s)	578, 680
MspI	CCGG	2 cut(s)	722, 777
MspR9I	CCNGG	2 cut(s)	723, 852
Mva1269I	GAATGC	1 cut(s)	388
MvaI	CCWGG	1 cut(s)	852
MvnI	CGCG	1 cut(s)	34
MwoI	GCNNNNNNNGC	2 cut(s)	96, 445
NciI	CCSGG	1 cut(s)	723
NdeI	CATATG	1 cut(s)	360
NdeII	GATC	2 cut(s)	313, 763
NheI	GCTAGC	1 cut(s)	818
NlaIII	CATG	5 cut(s)	448, 477, 637, 700, 883
NlaIV	GGNNCC	2 cut(s)	255, 765
NsiI	ATGCAT	1 cut(s)	494
PctI	GAATGC	1 cut(s)	388
PfeI	GAWTC	3 cut(s)	239, 266, 303
PkrI	GCNGC	4 cut(s)	128, 580, 591, 775
PleI	GAGTC	1 cut(s)	338
PpsI	GAGTC	1 cut(s)	338
Psp6I	CCWGG	1 cut(s)	850
PspGI	CCWGG	1 cut(s)	850
PspN4I	GGNNCC	2 cut(s)	255, 765
PspPI	GGNCC	1 cut(s)	863
PstI	CTGCAG	1 cut(s)	594
PsuI	RGATCY	1 cut(s)	763
PvuII	CAGCTG	2 cut(s)	578, 680
RsaI	GTAC	1 cut(s)	478
RsaNI	GTAC	1 cut(s)	477
RseI	CAYNNNNRTG	2 cut(s)	363, 878
SaqAI	TTAA	2 cut(s)	750, 899
SatI	GCNGC	4 cut(s)	127, 579, 590, 774
Sau3AI	GATC	2 cut(s)	313, 763
Sau96I	GGNCC	1 cut(s)	863
SchI	GAGTC	1 cut(s)	339
ScrFI	CCNGG	2 cut(s)	723, 852
SfaNI	GCATC	4 cut(s)	479, 501, 592, 839
SfcI	CTRYAG	1 cut(s)	590
SinI	GGWCC	1 cut(s)	863
SmiMI	CAYNNNNRTG	2 cut(s)	363, 878
Sse9I	AATT	5 cut(s)	92, 171, 521, 825, 915
SsiI	CCGC	5 cut(s)	55, 76, 386, 422, 787
SspI	AATATT	1 cut(s)	430
SspMI	CTAG	1 cut(s)	819
StyD4I	CCNGG	2 cut(s)	721, 850
TaaI	ACNGT	1 cut(s)	610
TaiI	ACGT	2 cut(s)	32, 408
TaqI	TCGA	3 cut(s)	219, 495, 738
TasI	AATT	5 cut(s)	92, 171, 521, 825, 915
TatI	WGTACW	1 cut(s)	476
TfiI	GAWTC	3 cut(s)	239, 266, 303
Tru1I	TTAA	2 cut(s)	750, 899
Tru9I	TTAA	2 cut(s)	750, 899
TseI	GCWGC	4 cut(s)	126, 578, 589, 773
TspDTI	ATGAA	4 cut(s)	248, 258, 381, 650
TspGWI	ACGGA	2 cut(s)	118, 342
VpaK11BI	GGWCC	1 cut(s)	863
XapI	RAATTY	2 cut(s)	521, 915
XmiI	GTMKAC	1 cut(s)	116
XspI	CTAG	1 cut(s)	819
Zsp2I	ATGCAT	1 cut(s)	494

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.