Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0013163

Physical Location & Seq

Genomic Coordinates Reverse (-)

258987 .. 261289

Gene Structure 2303 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G00620.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1083 bp

ATGGCGTCGATGATGTTTACCGATTGCTCCTCCACCACGACGTCGCGTCTCATCCATTTGAACCGAAGCAGCGGTACTTTCCTCCTCCGTCAATGCGTCGGCCAACTCCGTTTACAGACCACTGCTTCTGGCCGTGGCTGCTGCATTCGCTCCTCTTCTTCTCCCATTTCTTCCATTTCCGCTGATACAAAATCAGAGGGTGGCGCAATTGTAATAGATGGAAAGGCTGTGGCAAAAAAGATTAGAGATGAAATCACAATTGAAGTTTCAAGAATGAAGGAATCAATTGGTGTGATTCCTGGTTTAGCAGTAATCCTTGTTGGTGACAGAAAAGATTCTGCAACTTATGTGAGGAACAAGAAGAAAGCTTGTGACTCCGTTGGAATCAAATCGTTCGAAGTTCGTCTAGCTGAAGATTCATCAGAAGAAGAGGTGTTGAAATCTGTATCAGGATTCAATGATGATCCTTCTGTCCATGGAATCCTTGTTCAGTTGCCTCTGCCATCGCATATGGATGAACAGAACATACTGAATGCGGTTAGTATAGAGAAAGATGTTGACGGATTTCATCCGCTAAATATTGGACGGCTTGCCATGCGTGGAAGAGAACCCTTATTCGTTCCTTGTACTCCAAAAGGATGCATTGAGTTGTTGCATAGATACAACATTGAAATCAAAGGAAAGAGAGCGGTTGTTATCGGAAGGAGTAACATTGTCGGTATGCCAGCTGCTCTTTTACTGCAGAGGGAGGATGCAACCGTTAGCATTATCCATTCAAGAACCAAGAACCCTGAAGAAATCACAAGAGAAGCTGATATTATAATCTCAGCTGTTGGACAGCCAAACATGGTCAGAGGAAGCTGGATAAAACCGGGCGCAGTCCTCATCGATGTTGGGATTAATCCTGTTGAGGATCCAAGTGCTGCGCGTGGATATCGATTGGTTGGAGACATTTGCTACGAGGAGGCTAGCAAAGTTGCATCAGCCATCACACCTGTTCCTGGCGGTGTAGGACCAATGACCATAGCCATGCTTCTATCCAACACTTTAACATCAGCTAAGAGGATTCACAACTTCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004477 GO:0004488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006730 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0019238 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

360

Amino Acids

38.74

Weight (kDa)

8.58

Isoelectric Point (pI)

49.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THF_DHG_CYH	PF00763	72 - 187	2.8e-43	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C	PF02882	208 - 355	3.4e-62	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013163)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00600 AT4G00620
fragaria_vesca	FvH4_7g13650 FvH4_7g13650
malus_domestica	MD07G1141500.v1.1 MD08G1243500.v1.1
prunus_persica	Prupe.2G162600_v2.0.a1
pyrus_communis	pycom07g14040 pycom08g21120
rosa_chinensis	RchiOBHm_Chr1g0353731
rosa_laevigata	RLG00000028263
rosa_roxburghii	Rroxscaffold_4G00301710
rosa_rugosa	Rorug01G0237200
rosa_samantha	Rh1AG249400 Rh1BG219500 Rh1CG232100 Rh1DG246500
rosa_wichuraiana	Rw1G021760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	821
AatII	GACGTC	1 cut(s)	44
AccBSI	CCGCTC	1 cut(s)	689
AccII	CGCG	2 cut(s)	46, 928
AciI	CCGC	6 cut(s)	72, 180, 536, 572, 689, 1005
AclWI	GGATC	3 cut(s)	458, 908, 921
AcoI	YGGCCR	2 cut(s)	100, 130
AcuI	CTGAAG	2 cut(s)	432, 813
AcyI	GRCGYC	2 cut(s)	5, 41
AfaI	GTAC	2 cut(s)	76, 628
AfiI	CCNNNNNNNGG	1 cut(s)	1001
AgsI	TTSAA	7 cut(s)	61, 263, 270, 439, 457, 671, 777
AhdI	GACNNNNNGTC	1 cut(s)	45
AjnI	CCWGG	2 cut(s)	298, 1000
AloI	GAACNNNNNNTCC	2 cut(s)	471, 503
AluBI	AGCT	7 cut(s)	368, 410, 728, 812, 830, 861, 1058
AluI	AGCT	7 cut(s)	368, 410, 728, 812, 830, 861, 1058
Alw26I	GTCTC	2 cut(s)	53, 942
AlwI	GGATC	3 cut(s)	458, 908, 921
AoxI	GGCC	2 cut(s)	100, 130
ApeKI	GCWGC	5 cut(s)	69, 138, 141, 728, 923
AseI	ATTAAT	1 cut(s)	900
Asp700I	GAANNNNTTC	1 cut(s)	334
AspLEI	GCGC	3 cut(s)	206, 878, 928
AspS9I	GGNCC	1 cut(s)	1013
AsuC2I	CCSGG	1 cut(s)	873
AsuHPI	GGTGA	1 cut(s)	335
AsuII	TTCGAA	1 cut(s)	396
AsuNHI	GCTAGC	1 cut(s)	968
AvaII	GGWCC	1 cut(s)	1013
BamHI	GGATCC	1 cut(s)	913
BarI	GAAGNNNNNNTAC	2 cut(s)	58, 90
BbvI	GCAGC	5 cut(s)	81, 125, 128, 715, 910
BccI	CCATC	3 cut(s)	212, 511, 995
BceAI	ACGGC	2 cut(s)	117, 602
BciT130I	CCWGG	2 cut(s)	300, 1002
BcnI	CCSGG	1 cut(s)	873
BcoDI	GTCTC	2 cut(s)	53, 942
BfaI	CTAG	2 cut(s)	407, 969
BfmI	CTRYAG	1 cut(s)	740
BisI	GCNGC	5 cut(s)	70, 139, 142, 729, 924
BlsI	GCNGC	5 cut(s)	71, 140, 143, 730, 925
Bme1390I	CCNGG	3 cut(s)	300, 873, 1002
Bme18I	GGWCC	1 cut(s)	1013
BmeRI	GACNNNNNGTC	1 cut(s)	45
BmgT120I	GGNCC	1 cut(s)	1013
BmiI	GGNNCC	1 cut(s)	915
BmrFI	CCNGG	3 cut(s)	300, 873, 1002
BmsI	GCATC	3 cut(s)	629, 742, 989
BmtI	GCTAGC	1 cut(s)	972
Bpu14I	TTCGAA	1 cut(s)	396
BpuMI	CCSGG	1 cut(s)	873
Bsa29I	ATCGAT	2 cut(s)	888, 937
BsaHI	GRCGYC	2 cut(s)	5, 41
BsaJI	CCNNGG	2 cut(s)	133, 475
Bsc4I	CCNNNNNNNGG	1 cut(s)	1001
Bse1I	ACTGG	1 cut(s)	1078
BseBI	CCWGG	2 cut(s)	300, 1002
BseCI	ATCGAT	2 cut(s)	888, 937
BseDI	CCNNGG	2 cut(s)	133, 475
BseGI	GGATG	5 cut(s)	51, 520, 568, 644, 757
BseLI	CCNNNNNNNGG	1 cut(s)	1001
BseMII	CTCAG	1 cut(s)	840
BseNI	ACTGG	1 cut(s)	1078
BseRI	GAGGAG	4 cut(s)	19, 74, 142, 977
BseXI	GCAGC	5 cut(s)	81, 125, 128, 715, 910
Bsh1236I	CGCG	2 cut(s)	46, 928
BshFI	GGCC	2 cut(s)	102, 132
BshVI	ATCGAT	2 cut(s)	888, 937
BsiSI	CCGG	1 cut(s)	872
BslI	CCNNNNNNNGG	1 cut(s)	1001
BsmAI	GTCTC	2 cut(s)	53, 942
BsmBI	CGTCTC	1 cut(s)	53
BsmI	GAATGC	2 cut(s)	144, 538
BsnI	GGCC	2 cut(s)	102, 132
Bsp119I	TTCGAA	1 cut(s)	396
Bsp143I	GATC	2 cut(s)	463, 913
Bsp19I	CCATGG	1 cut(s)	475
BspACI	CCGC	6 cut(s)	72, 180, 536, 572, 689, 1005
BspANI	GGCC	2 cut(s)	102, 132
BspCNI	CTCAG	1 cut(s)	839
BspDI	ATCGAT	2 cut(s)	888, 937
BspFNI	CGCG	2 cut(s)	46, 928
BspLI	GGNNCC	1 cut(s)	915
BspMAI	CTGCAG	1 cut(s)	744
BspOI	GCTAGC	1 cut(s)	972
BspPI	GGATC	3 cut(s)	458, 908, 921
BspT104I	TTCGAA	1 cut(s)	396
BsrBI	CCGCTC	1 cut(s)	689
BsrI	ACTGG	1 cut(s)	1078
BssECI	CCNNGG	2 cut(s)	133, 475
BssMI	GATC	2 cut(s)	463, 913
BssNI	GRCGYC	2 cut(s)	5, 41
BssT1I	CCWWGG	1 cut(s)	475
Bst2UI	CCWGG	2 cut(s)	300, 1002
Bst4CI	ACNGT	1 cut(s)	760
Bst6I	CTCTTC	3 cut(s)	160, 423, 598
BstACI	GRCGYC	2 cut(s)	5, 41
BstBI	TTCGAA	1 cut(s)	396
BstC8I	GCNNGC	3 cut(s)	591, 726, 970
BstDEI	CTNAG	2 cut(s)	826, 1059
BstDSI	CCRYGG	2 cut(s)	133, 475
BstF5I	GGATG	5 cut(s)	51, 520, 568, 644, 757
BstFNI	CGCG	2 cut(s)	46, 928
BstHHI	GCGC	3 cut(s)	206, 878, 928
BstKTI	GATC	2 cut(s)	466, 916
BstMAI	GTCTC	2 cut(s)	53, 942
BstMBI	GATC	2 cut(s)	463, 913
BstMWI	GCNNNNNNNGC	3 cut(s)	138, 147, 595
BstNI	CCWGG	2 cut(s)	300, 1002
BstSCI	CCNGG	3 cut(s)	298, 871, 1000
BstSFI	CTRYAG	1 cut(s)	740
BstUI	CGCG	2 cut(s)	46, 928
BstV1I	GCAGC	5 cut(s)	81, 125, 128, 715, 910
BstX2I	RGATCY	1 cut(s)	913
BstYI	RGATCY	1 cut(s)	913
Bsu15I	ATCGAT	2 cut(s)	888, 937
BsuRI	GGCC	2 cut(s)	102, 132
BsuTUI	ATCGAT	2 cut(s)	888, 937
BtgI	CCRYGG	2 cut(s)	133, 475
BtgZI	GCGATG	1 cut(s)	489
BtsCI	GGATG	5 cut(s)	51, 520, 568, 644, 757
BtsI	GCAGTG	1 cut(s)	120
BtsIMutI	CAGTG	1 cut(s)	120
Cac8I	GCNNGC	3 cut(s)	591, 726, 970
CfoI	GCGC	3 cut(s)	206, 878, 928
Cfr13I	GGNCC	1 cut(s)	1013
ClaI	ATCGAT	2 cut(s)	888, 937
CseI	GACGC	2 cut(s)	35, 85
Csp6I	GTAC	2 cut(s)	75, 627
CviAII	CATG	4 cut(s)	476, 595, 847, 1030
CviQI	GTAC	2 cut(s)	75, 627
DdeI	CTNAG	2 cut(s)	826, 1059
DpnI	GATC	2 cut(s)	465, 915
DpnII	GATC	2 cut(s)	463, 913
DriI	GACNNNNNGTC	1 cut(s)	45
EaeI	YGGCCR	2 cut(s)	100, 130
Eam1104I	CTCTTC	3 cut(s)	160, 423, 598
Eam1105I	GACNNNNNGTC	1 cut(s)	45
EarI	CTCTTC	3 cut(s)	160, 423, 598
Eco130I	CCWWGG	1 cut(s)	475
Eco32I	GATATC	1 cut(s)	935
Eco47I	GGWCC	1 cut(s)	1013
Eco57I	CTGAAG	2 cut(s)	432, 813
EcoRII	CCWGG	2 cut(s)	298, 1000
EcoRV	GATATC	1 cut(s)	935
EcoT14I	CCWWGG	1 cut(s)	475
EcoT22I	ATGCAT	1 cut(s)	644
ErhI	CCWWGG	1 cut(s)	475
Esp3I	CGTCTC	1 cut(s)	53
FaeI	CATG	4 cut(s)	479, 598, 850, 1033
FatI	CATG	4 cut(s)	475, 594, 846, 1029
FauNDI	CATATG	1 cut(s)	510
Fnu4HI	GCNGC	5 cut(s)	70, 139, 142, 729, 924
FokI	GGATG	5 cut(s)	38, 527, 555, 651, 764
Fsp4HI	GCNGC	5 cut(s)	70, 139, 142, 729, 924
FspBI	CTAG	2 cut(s)	407, 969
GlaI	GCGC	3 cut(s)	205, 877, 927
GluI	GCNGC	5 cut(s)	70, 139, 142, 729, 924
HaeIII	GGCC	2 cut(s)	102, 132
HapII	CCGG	1 cut(s)	872
HgaI	GACGC	2 cut(s)	35, 85
HhaI	GCGC	3 cut(s)	206, 878, 928
Hin1I	GRCGYC	2 cut(s)	5, 41
Hin1II	CATG	4 cut(s)	479, 598, 850, 1033
Hin6I	GCGC	3 cut(s)	204, 876, 926
HinP1I	GCGC	3 cut(s)	204, 876, 926
HincII	GTYRAC	1 cut(s)	559
HindII	GTYRAC	1 cut(s)	559
HindIII	AAGCTT	1 cut(s)	366
HinfI	GANTC	9 cut(s)	281, 295, 335, 374, 384, 416, 453, 480, 1066
HpaII	CCGG	1 cut(s)	872
HphI	GGTGA	1 cut(s)	335
Hpy166II	GTNNAC	3 cut(s)	18, 113, 559
Hpy188I	TCNGA	4 cut(s)	196, 424, 701, 854
Hpy188III	TCNNGA	3 cut(s)	270, 450, 777
Hpy8I	GTNNAC	3 cut(s)	18, 113, 559
Hpy99I	CGWCG	4 cut(s)	10, 43, 46, 101
HpyAV	CCTTC	3 cut(s)	271, 477, 696
HpyCH4III	ACNGT	1 cut(s)	760
HpyCH4IV	ACGT	1 cut(s)	41
HpyCH4V	TGCA	7 cut(s)	144, 341, 642, 655, 742, 755, 980
HpyF10VI	GCNNNNNNNGC	3 cut(s)	138, 147, 595
HpyF3I	CTNAG	2 cut(s)	826, 1059
HpySE526I	ACGT	1 cut(s)	41
Hsp92I	GRCGYC	2 cut(s)	5, 41
Hsp92II	CATG	4 cut(s)	479, 598, 850, 1033
HspAI	GCGC	3 cut(s)	204, 876, 926
Kzo9I	GATC	2 cut(s)	463, 913
LmnI	GCTCC	2 cut(s)	32, 155
Lsp1109I	GCAGC	5 cut(s)	81, 125, 128, 715, 910
LweI	GCATC	3 cut(s)	629, 742, 989
MaeI	CTAG	2 cut(s)	407, 969
MaeII	ACGT	1 cut(s)	41
MaeIII	GTNAC	3 cut(s)	323, 371, 707
MalI	GATC	2 cut(s)	465, 915
MbiI	CCGCTC	1 cut(s)	689
MboI	GATC	2 cut(s)	463, 913
MboII	GAAGA	9 cut(s)	147, 150, 162, 373, 425, 437, 440, 615, 806
MfeI	CAATTG	3 cut(s)	207, 258, 285
MflI	RGATCY	1 cut(s)	913
MluCI	AATT	3 cut(s)	207, 258, 285
MlyI	GAGTC	1 cut(s)	368
MmeI	TCCRAC	4 cut(s)	361, 814, 925, 1065
Mph1103I	ATGCAT	1 cut(s)	644
MroXI	GAANNNNTTC	1 cut(s)	334
MseI	TTAA	2 cut(s)	900, 1049
MslI	CAYNNNNRTG	2 cut(s)	513, 1028
MspA1I	CMGCKG	4 cut(s)	72, 182, 728, 830
MspI	CCGG	1 cut(s)	872
MspR9I	CCNGG	3 cut(s)	300, 873, 1002
MunI	CAATTG	3 cut(s)	207, 258, 285
Mva1269I	GAATGC	2 cut(s)	144, 538
MvaI	CCWGG	2 cut(s)	300, 1002
MvnI	CGCG	2 cut(s)	46, 928
MwoI	GCNNNNNNNGC	3 cut(s)	138, 147, 595
NciI	CCSGG	1 cut(s)	873
NcoI	CCATGG	1 cut(s)	475
NdeI	CATATG	1 cut(s)	510
NdeII	GATC	2 cut(s)	463, 913
NheI	GCTAGC	1 cut(s)	968
NlaIII	CATG	4 cut(s)	479, 598, 850, 1033
NlaIV	GGNNCC	1 cut(s)	915
NmuCI	GTSAC	2 cut(s)	323, 371
NsiI	ATGCAT	1 cut(s)	644
NspV	TTCGAA	1 cut(s)	396
PctI	GAATGC	2 cut(s)	144, 538
PdmI	GAANNNNTTC	1 cut(s)	334
PfeI	GAWTC	8 cut(s)	281, 295, 335, 384, 416, 453, 480, 1066
PkrI	GCNGC	5 cut(s)	71, 140, 143, 730, 925
PleI	GAGTC	1 cut(s)	368
PpsI	GAGTC	1 cut(s)	368
PshBI	ATTAAT	1 cut(s)	900
PsiI	TTATAA	1 cut(s)	821
Psp6I	CCWGG	2 cut(s)	298, 1000
PspGI	CCWGG	2 cut(s)	298, 1000
PspN4I	GGNNCC	1 cut(s)	915
PspPI	GGNCC	1 cut(s)	1013
PsrI	GAACNNNNNNTAC	2 cut(s)	510, 542
PstI	CTGCAG	1 cut(s)	744
PsuI	RGATCY	1 cut(s)	913
PvuII	CAGCTG	2 cut(s)	728, 830
RsaI	GTAC	2 cut(s)	76, 628
RsaNI	GTAC	2 cut(s)	75, 627
RseI	CAYNNNNRTG	2 cut(s)	513, 1028
SaqAI	TTAA	2 cut(s)	900, 1049
SatI	GCNGC	5 cut(s)	70, 139, 142, 729, 924
Sau3AI	GATC	2 cut(s)	463, 913
Sau96I	GGNCC	1 cut(s)	1013
SchI	GAGTC	1 cut(s)	368
ScrFI	CCNGG	3 cut(s)	300, 873, 1002
SfaNI	GCATC	3 cut(s)	629, 742, 989
SfcI	CTRYAG	1 cut(s)	740
SfuI	TTCGAA	1 cut(s)	396
SinI	GGWCC	1 cut(s)	1013
SmiMI	CAYNNNNRTG	2 cut(s)	513, 1028
Sse9I	AATT	3 cut(s)	207, 258, 285
SsiI	CCGC	6 cut(s)	72, 180, 536, 572, 689, 1005
SspI	AATATT	1 cut(s)	580
SspMI	CTAG	2 cut(s)	407, 969
StyD4I	CCNGG	3 cut(s)	298, 871, 1000
StyI	CCWWGG	1 cut(s)	475
TaaI	ACNGT	1 cut(s)	760
TaiI	ACGT	1 cut(s)	44
TaqI	TCGA	4 cut(s)	8, 396, 888, 937
TasI	AATT	3 cut(s)	207, 258, 285
TatI	WGTACW	1 cut(s)	626
TfiI	GAWTC	8 cut(s)	281, 295, 335, 384, 416, 453, 480, 1066
Tru1I	TTAA	2 cut(s)	900, 1049
Tru9I	TTAA	2 cut(s)	900, 1049
TscAI	CASTG	1 cut(s)	127
TseFI	GTSAC	2 cut(s)	323, 371
TseI	GCWGC	5 cut(s)	69, 138, 141, 728, 923
Tsp45I	GTSAC	2 cut(s)	323, 371
TspDTI	ATGAA	5 cut(s)	264, 290, 408, 531, 557
TspGWI	ACGGA	4 cut(s)	77, 98, 367, 576
TspRI	CASTG	1 cut(s)	127
VpaK11BI	GGWCC	1 cut(s)	1013
VspI	ATTAAT	1 cut(s)	900
XmnI	GAANNNNTTC	1 cut(s)	334
XspI	CTAG	2 cut(s)	407, 969
ZraI	GACGTC	1 cut(s)	42
Zsp2I	ATGCAT	1 cut(s)	644

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.