Prupe.2G162600_v2.0.a1

Bifunctional protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0013163
Physical Location & Seq
Reverse (-)
21314558 .. 21318283
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G162600.1

Sequence Viewer

Length: 1083 bp
ATGGCGTCGATGATGTTCACGGACTGCTCGTCCTCCACCACCGCTCGGCTCCTCCCCTTCAGACACAACCTTCTCCTCCGCAGAAATAACGGCGTCGTTTCCCTTCGCCGCCTCATAAACCTTGGCTCTCCCAGAAGCCTCCGCGTTCACTCCTCTTCTTCACTCTCTCCATCACCCACTGCTTCATTGGCGACTGAAACACCGGCTAAAGTGATTGATGGAAAATCAGTTGCGAAGCAAATCAGAGATGAGATCAATGCTGAAGTGGCGAGAATGAAGGATGCAATTGGAATTGTTCCGGGTTTAGCAGTTATTCTTGTCGGGGACAGGAAGGACTCTGCCACTTATGTGCGCAACAAGAAAAAAGCTTGTGATTCTGTCGGGATTAATTCATTCGAAGTGCGTTTGCCAGAGGATTCCACAGAACAAGAAGTGCTCAAATTCATCTCGGGCTTCAATGATGACTCCTCAGTTCATGGCATCCTTGTTCAGTTACCTCTGCCTCCTCATATGAATGAGCAAAACATCTTAAATGCTGTTAGCATTGAGAAAGATGTGGATGGGTTTCACCCGCTAAATATTGGTCGTCTTGCCATGCGTGGAAGGGATCCCTTGTTTGTGCCTTGTACACCAAAAGGGTGCATAGAGTTGTTGCATAGGTATGGTGTACCTATCAAAGGAAAGAGAGCAGTTGTAATTGGACGGAGCAATATTGTTGGGATGCCAGCTGCTTTATTGCTGCAGAGGGAAGATGCTACAATCAGTATAGTCCATTCCCGAACCAAGAATCCTGAGGAGATCACAAGACAAGCAGATATCATAATCTCAGCTGTAGGGCAACCAAATATGGTGAGGGGTAGCTGGATTAAACCTGGTGCAGTGATTATTGATGTCGGAATAAATCCAGTTGAGGATGAAAAGAGCCCTCGAGGTTATCGGCTGGTTGGAGATGTCTGCTATGAGGAGGCCAGCCAGATTGCTTCAGCAATTACTCCAGTACCTGGGGGAGTGGGTCCAATGACAATAGCAATGCTTCTCTCTAATACTCTCGTATCAGCAAAAAGGGTACACAACTTCCAGTGA

Protein Analysis

361

Amino Acids

39.0

Weight (kDa)

9.31

Isoelectric Point (pI)

53.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00600 AT4G00620
fragaria_vesca FvH4_7g13650 FvH4_7g13650
malus_domestica MD07G1141500.v1.1 MD08G1243500.v1.1
prunus_persica Prupe.2G162600_v2.0.a1
pyrus_communis pycom07g14040 pycom08g21120
rosa_chinensis RchiOBHm_Chr1g0353731
rosa_laevigata RLG00000028263
rosa_roxburghii Rroxscaffold_4G00301710
rosa_rugosa Rorug01G0237200
rosa_samantha Rh1AG249400 Rh1BG219500 Rh1CG232100 Rh1DG246500
rosa_wichuraiana Rw1G021760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 927
Acc16I TGCGCA 1 cut(s) 353
AccB7I CCANNNNNTGG 1 cut(s) 1001
AccBSI CCGCTC 1 cut(s) 44
AccII CGCG 1 cut(s) 144
AciI CCGC 5 cut(s) 42, 79, 109, 142, 572
AclWI GGATC 2 cut(s) 602, 615
AcsI RAATTY 1 cut(s) 440
AcuI CTGAAG 3 cut(s) 43, 282, 966
AcyI GRCGYC 2 cut(s) 5, 93
AfaI GTAC 4 cut(s) 628, 669, 999, 1068
AfiI CCNNNNNNNGG 3 cut(s) 45, 677, 1001
AgsI TTSAA 1 cut(s) 457
AhdI GACNNNNNGTC 1 cut(s) 28
AjnI CCWGG 2 cut(s) 871, 1000
AleI CACNNNNGTG 1 cut(s) 347
AloI GAACNNNNNNTCC 2 cut(s) 772, 804
AluBI AGCT 4 cut(s) 368, 728, 830, 861
AluI AGCT 4 cut(s) 368, 728, 830, 861
Alw21I GWGCWC 1 cut(s) 438
AlwI GGATC 2 cut(s) 602, 615
AlwNI CAGNNNCTG 1 cut(s) 1001
Ama87I CYCGRG 2 cut(s) 448, 927
AoxI GGCC 1 cut(s) 966
ApeKI GCWGC 2 cut(s) 728, 739
ApoI RAATTY 1 cut(s) 440
AseI ATTAAT 1 cut(s) 387
AspLEI GCGC 1 cut(s) 354
AspS9I GGNCC 1 cut(s) 1013
AsuC2I CCSGG 1 cut(s) 300
AsuHPI GGTGA 3 cut(s) 165, 560, 862
AsuII TTCGAA 1 cut(s) 396
AvaI CYCGRG 2 cut(s) 448, 927
AvaII GGWCC 1 cut(s) 1013
AxyI CCTNAGG 1 cut(s) 792
BaeI ACNNNNGTAYC 4 cut(s) 981, 1014, 1035, 1068
BamHI GGATCC 1 cut(s) 607
BanII GRGCYC 1 cut(s) 926
Bbv12I GWGCWC 1 cut(s) 438
BbvI GCAGC 2 cut(s) 715, 726
BccI CCATC 3 cut(s) 178, 212, 554
BceAI ACGGC 1 cut(s) 106
BciT130I CCWGG 2 cut(s) 873, 1002
BcnI CCSGG 1 cut(s) 300
BfmI CTRYAG 2 cut(s) 740, 831
BisI GCNGC 3 cut(s) 109, 729, 740
BlsI GCNGC 3 cut(s) 110, 730, 741
Bme1390I CCNGG 3 cut(s) 300, 873, 1002
Bme18I GGWCC 1 cut(s) 1013
BmeRI GACNNNNNGTC 1 cut(s) 28
BmeT110I CYCGRG 2 cut(s) 448, 927
BmgT120I GGNCC 1 cut(s) 1013
BmiI GGNNCC 3 cut(s) 50, 609, 1014
BmrFI CCNGG 3 cut(s) 300, 873, 1002
BmsI GCATC 4 cut(s) 271, 489, 711, 742
BpmI CTGGAG 1 cut(s) 978
Bpu14I TTCGAA 1 cut(s) 396
BpuMI CCSGG 1 cut(s) 300
BsaHI GRCGYC 2 cut(s) 5, 93
BsaJI CCNNGG 2 cut(s) 121, 1001
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 677, 1001
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 3 cut(s) 905, 995, 1078
Bse21I CCTNAGG 1 cut(s) 792
Bse3DI GCAATG 1 cut(s) 1035
BseBI CCWGG 2 cut(s) 873, 1002
BseDI CCNNGG 2 cut(s) 121, 1001
BseGI GGATG 5 cut(s) 286, 480, 565, 726, 919
BseLI CCNNNNNNNGG 3 cut(s) 45, 677, 1001
BseMI GCAATG 1 cut(s) 1035
BseMII CTCAG 3 cut(s) 483, 783, 840
BseNI ACTGG 3 cut(s) 905, 995, 1078
BseRI GAGGAG 7 cut(s) 41, 65, 142, 457, 495, 809, 977
BseXI GCAGC 2 cut(s) 715, 726
BsgI GTGCAG 1 cut(s) 897
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 1 cut(s) 968
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 2 cut(s) 448, 927
BsiSI CCGG 2 cut(s) 203, 299
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 3 cut(s) 45, 677, 1001
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 1 cut(s) 968
BsoBI CYCGRG 2 cut(s) 448, 927
Bsp119I TTCGAA 1 cut(s) 396
Bsp1286I GDGCHC 2 cut(s) 438, 926
Bsp1407I TGTACA 1 cut(s) 626
Bsp143I GATC 3 cut(s) 252, 607, 798
BspACI CCGC 5 cut(s) 42, 79, 109, 142, 572
BspANI GGCC 1 cut(s) 968
BspCNI CTCAG 3 cut(s) 482, 784, 839
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 3 cut(s) 50, 609, 1014
BspMAI CTGCAG 1 cut(s) 744
BspPI GGATC 2 cut(s) 602, 615
BspT104I TTCGAA 1 cut(s) 396
BsrBI CCGCTC 1 cut(s) 44
BsrDI GCAATG 1 cut(s) 1035
BsrFI RCCGGY 1 cut(s) 202
BsrGI TGTACA 1 cut(s) 626
BsrI ACTGG 3 cut(s) 905, 995, 1078
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 2 cut(s) 121, 1001
BssMI GATC 3 cut(s) 252, 607, 798
BssNI GRCGYC 2 cut(s) 5, 93
BssT1I CCWWGG 1 cut(s) 121
Bst2UI CCWGG 2 cut(s) 873, 1002
Bst6I CTCTTC 1 cut(s) 160
BstACI GRCGYC 2 cut(s) 5, 93
BstAUI TGTACA 1 cut(s) 626
BstBI TTCGAA 1 cut(s) 396
BstC8I GCNNGC 2 cut(s) 726, 970
BstDEI CTNAG 3 cut(s) 469, 792, 826
BstENI CCTNNNNNAGG 1 cut(s) 675
BstF5I GGATG 5 cut(s) 286, 480, 565, 726, 919
BstFNI CGCG 1 cut(s) 144
BstHHI GCGC 1 cut(s) 354
BstKTI GATC 3 cut(s) 255, 610, 801
BstMBI GATC 3 cut(s) 252, 607, 798
BstMWI GCNNNNNNNGC 2 cut(s) 188, 266
BstNI CCWGG 2 cut(s) 873, 1002
BstSCI CCNGG 3 cut(s) 298, 871, 1000
BstSFI CTRYAG 2 cut(s) 740, 831
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 2 cut(s) 715, 726
BstX2I RGATCY 1 cut(s) 607
BstYI RGATCY 1 cut(s) 607
Bsu36I CCTNAGG 1 cut(s) 792
BsuRI GGCC 1 cut(s) 968
BtsCI GGATG 5 cut(s) 286, 480, 565, 726, 919
BtsI GCAGTG 2 cut(s) 177, 885
BtsIMutI CAGTG 2 cut(s) 177, 885
Cac8I GCNNGC 2 cut(s) 726, 970
CaiI CAGNNNCTG 1 cut(s) 1001
CfoI GCGC 1 cut(s) 354
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 1013
CseI GACGC 1 cut(s) 82
CsiI ACCWGGT 1 cut(s) 871
Csp6I GTAC 4 cut(s) 627, 668, 998, 1067
CviAII CATG 2 cut(s) 476, 595
CviQI GTAC 4 cut(s) 627, 668, 998, 1067
DdeI CTNAG 3 cut(s) 469, 792, 826
DpnI GATC 3 cut(s) 254, 609, 800
DpnII GATC 3 cut(s) 252, 607, 798
DriI GACNNNNNGTC 1 cut(s) 28
Eam1104I CTCTTC 1 cut(s) 160
Eam1105I GACNNNNNGTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 121
Eco24I GRGCYC 1 cut(s) 926
Eco32I GATATC 1 cut(s) 817
Eco47I GGWCC 1 cut(s) 1013
Eco57I CTGAAG 3 cut(s) 43, 282, 966
Eco81I CCTNAGG 1 cut(s) 792
Eco88I CYCGRG 2 cut(s) 448, 927
EcoNI CCTNNNNNAGG 1 cut(s) 675
EcoRII CCWGG 2 cut(s) 871, 1000
EcoRV GATATC 1 cut(s) 817
EcoT14I CCWWGG 1 cut(s) 121
EcoT38I GRGCYC 1 cut(s) 926
ErhI CCWWGG 1 cut(s) 121
FaeI CATG 2 cut(s) 479, 598
FaqI GGGAC 1 cut(s) 338
FatI CATG 2 cut(s) 475, 594
FauI CCCGC 1 cut(s) 579
FauNDI CATATG 1 cut(s) 510
Fnu4HI GCNGC 3 cut(s) 109, 729, 740
FokI GGATG 5 cut(s) 293, 467, 572, 733, 926
FriOI GRGCYC 1 cut(s) 926
Fsp4HI GCNGC 3 cut(s) 109, 729, 740
FspI TGCGCA 1 cut(s) 353
GlaI GCGC 1 cut(s) 353
GluI GCNGC 3 cut(s) 109, 729, 740
GsuI CTGGAG 1 cut(s) 978
HaeIII GGCC 1 cut(s) 968
HapII CCGG 2 cut(s) 203, 299
HgaI GACGC 1 cut(s) 82
HhaI GCGC 1 cut(s) 354
Hin1I GRCGYC 2 cut(s) 5, 93
Hin1II CATG 2 cut(s) 479, 598
Hin6I GCGC 1 cut(s) 352
HinP1I GCGC 1 cut(s) 352
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 5 cut(s) 335, 374, 416, 464, 787
HpaII CCGG 2 cut(s) 203, 299
HphI GGTGA 3 cut(s) 165, 560, 862
Hpy166II GTNNAC 5 cut(s) 18, 148, 629, 668, 1069
Hpy188I TCNGA 3 cut(s) 62, 245, 896
Hpy188III TCNNGA 3 cut(s) 382, 777, 791
Hpy8I GTNNAC 5 cut(s) 18, 148, 629, 668, 1069
Hpy99I CGWCG 2 cut(s) 10, 98
HpyAV CCTTC 6 cut(s) 67, 80, 113, 271, 325, 597
HpyCH4V TGCA 5 cut(s) 284, 642, 655, 742, 878
HpyF10VI GCNNNNNNNGC 2 cut(s) 188, 266
HpyF3I CTNAG 3 cut(s) 469, 792, 826
Hsp92I GRCGYC 2 cut(s) 5, 93
Hsp92II CATG 2 cut(s) 479, 598
HspAI GCGC 1 cut(s) 352
Kzo9I GATC 3 cut(s) 252, 607, 798
LmnI GCTCC 2 cut(s) 54, 705
Lsp1109I GCAGC 2 cut(s) 715, 726
LweI GCATC 4 cut(s) 271, 489, 711, 742
MabI ACCWGGT 1 cut(s) 871
MaeIII GTNAC 1 cut(s) 492
MalI GATC 3 cut(s) 254, 609, 800
MbiI CCGCTC 1 cut(s) 44
MboI GATC 3 cut(s) 252, 607, 798
MboII GAAGA 3 cut(s) 147, 150, 761
MfeI CAATTG 1 cut(s) 285
MflI RGATCY 1 cut(s) 607
MhlI GDGCHC 2 cut(s) 438, 926
MluCI AATT 6 cut(s) 285, 291, 388, 440, 696, 987
MlyI GAGTC 2 cut(s) 329, 458
MmeI TCCRAC 2 cut(s) 874, 925
MseI TTAA 3 cut(s) 387, 530, 867
MslI CAYNNNNRTG 3 cut(s) 347, 513, 660
MspA1I CMGCKG 2 cut(s) 728, 830
MspI CCGG 2 cut(s) 203, 299
MspR9I CCNGG 3 cut(s) 300, 873, 1002
MunI CAATTG 1 cut(s) 285
MvaI CCWGG 2 cut(s) 873, 1002
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 2 cut(s) 188, 266
NciI CCSGG 1 cut(s) 300
NdeI CATATG 1 cut(s) 510
NdeII GATC 3 cut(s) 252, 607, 798
NlaIII CATG 2 cut(s) 479, 598
NlaIV GGNNCC 3 cut(s) 50, 609, 1014
NmeAIII GCCGAG 1 cut(s) 25
NsbI TGCGCA 1 cut(s) 353
NspV TTCGAA 1 cut(s) 396
OliI CACNNNNGTG 1 cut(s) 347
PaeR7I CTCGAG 1 cut(s) 927
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PfeI GAWTC 3 cut(s) 374, 416, 787
PflMI CCANNNNNTGG 1 cut(s) 1001
PkrI GCNGC 3 cut(s) 110, 730, 741
PleI GAGTC 2 cut(s) 329, 458
PpsI GAGTC 2 cut(s) 329, 458
PshBI ATTAAT 1 cut(s) 387
Psp6I CCWGG 2 cut(s) 871, 1000
PspGI CCWGG 2 cut(s) 871, 1000
PspN4I GGNNCC 3 cut(s) 50, 609, 1014
PspPI GGNCC 1 cut(s) 1013
PspXI VCTCGAGB 1 cut(s) 927
PstI CTGCAG 1 cut(s) 744
PstNI CAGNNNCTG 1 cut(s) 1001
PsuI RGATCY 1 cut(s) 607
PvuII CAGCTG 2 cut(s) 728, 830
RsaI GTAC 4 cut(s) 628, 669, 999, 1068
RsaNI GTAC 4 cut(s) 627, 668, 998, 1067
RseI CAYNNNNRTG 3 cut(s) 347, 513, 660
SaqAI TTAA 3 cut(s) 387, 530, 867
SatI GCNGC 3 cut(s) 109, 729, 740
Sau3AI GATC 3 cut(s) 252, 607, 798
Sau96I GGNCC 1 cut(s) 1013
SchI GAGTC 2 cut(s) 329, 458
ScrFI CCNGG 3 cut(s) 300, 873, 1002
SduI GDGCHC 2 cut(s) 438, 926
SexAI ACCWGGT 1 cut(s) 871
SfaNI GCATC 4 cut(s) 271, 489, 711, 742
SfcI CTRYAG 2 cut(s) 740, 831
Sfr274I CTCGAG 1 cut(s) 927
SfuI TTCGAA 1 cut(s) 396
SinI GGWCC 1 cut(s) 1013
SlaI CTCGAG 1 cut(s) 927
SmiMI CAYNNNNRTG 3 cut(s) 347, 513, 660
SmlI CTYRAG 1 cut(s) 927
SmoI CTYRAG 1 cut(s) 927
Sse9I AATT 6 cut(s) 285, 291, 388, 440, 696, 987
SsiI CCGC 5 cut(s) 42, 79, 109, 142, 572
SspI AATATT 2 cut(s) 580, 712
StyD4I CCNGG 3 cut(s) 298, 871, 1000
StyI CCWWGG 1 cut(s) 121
TaqI TCGA 3 cut(s) 8, 396, 928
TasI AATT 6 cut(s) 285, 291, 388, 440, 696, 987
TatI WGTACW 1 cut(s) 626
TauI GCSGC 1 cut(s) 111
TfiI GAWTC 3 cut(s) 374, 416, 787
Tru1I TTAA 3 cut(s) 387, 530, 867
Tru9I TTAA 3 cut(s) 387, 530, 867
TscAI CASTG 2 cut(s) 184, 885
TseI GCWGC 2 cut(s) 728, 739
TspDTI ATGAA 7 cut(s) 174, 290, 381, 433, 464, 527, 930
TspGWI ACGGA 2 cut(s) 35, 718
TspRI CASTG 2 cut(s) 184, 885
Van91I CCANNNNNTGG 1 cut(s) 1001
VpaK11BI GGWCC 1 cut(s) 1013
VspI ATTAAT 1 cut(s) 387
XagI CCTNNNNNAGG 1 cut(s) 675
XapI RAATTY 1 cut(s) 440
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
XhoI CTCGAG 1 cut(s) 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.