Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0013163

Physical Location & Seq

Genomic Coordinates Forward (+)

20676281 .. 20679042

Gene Structure 2762 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000028263

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1134 bp

ATGGCGGCAGCGTCGATGGTATTCACGGACTGCTCCTCCACCACCGCCGCCGCTCGCCTCCTCCCCTTCACACGTACCACCCCTCTCCTCCTCCGCACCACCCAATACGCCGTCGTTTTCCCCCGCCGCCTCATCCACCCTCCCAGAACCCTCTCAATCCACTCCTCTTCTTTTTCACCCTCCCAATTGTCTGCCGCTTCAATTGTTTCCCAAAATGAGAAGCACGAAAAATCGGATACGAAGCTGGAAATAGTGAATAAAAGGCTTGATGGGAAGAAAGTGGCAGAGAAAATTAGAGGGGAGATAGCTGAGGAAGTGTCAAGGATGAAGGATGCAATTGGGGTTGTTCCGGGTTTGGCTGTTATTTTGGTTGGAGATAGGGAAGAATCTGCCACTTATGTTAGCAAGAAGAAGGAGGCTTGTGAGGTTGTGGGGATTACTTCTTTTGAAGTGCATTTGCCGGAGGATGCCACGGAACAAGAAGTGCTCGACTCCATCTCCGGCTTCAATGAGGATCCCTCAGTTCATGGCATCCTTGTTCAGTTACCTCTGCCTAGGCATATGGATGAACAAAACGTTTTAAATGCTGTTAGCATTGAGAAAGATGTGGATGGATTTCACCCGCTAAATATTGGCTGTCTTGCCATGCGAGGTAGGGAACCCTTCTTTGTTCCCTGTACTCCCAAAGGCTGCATAGAGCTGTTGCATAGATATGACATCCCTATCAAAGGAAAGAGAGCTGTTGTAATTGGCCGGAGCAATATTGTTGGGATGCCAGTTGCTTTGTTGCTGCAGAGGGAAGATGCTACAGTCAGCATAGTCCATTCCCGAACCAAGAATCCCGAAGAGTTAACAAAACAAGCAGATATTGTAATCGCAGCTGTAGGGCAAGCAAATATGGTGAGGGGTAGTTGGATTAAACCTGGTGCAGTAGTTATTGATGTCGGAATCAATCCAGTTGAGGATAAAACAAAGCGTTCGGGCTTTCGGCTAGTTGGAGATGTCTGTTATAAGGAGGCCAAAGAAATTGCTTCAGCAATTACTCCAGTACCAGGAGGAGTTGGTCCAATGACTATAGCAATGCTTCTCACTAATACACTCATATCAGCAAAAAGGATACACAACTCCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004477 GO:0004488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006730 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0019238 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

378

Amino Acids

40.95

Weight (kDa)

8.16

Isoelectric Point (pI)

46.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THF_DHG_CYH	PF00763	89 - 204	5.3e-43	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C	PF02882	225 - 372	2.6e-62	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013163)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00600 AT4G00620
fragaria_vesca	FvH4_7g13650 FvH4_7g13650
malus_domestica	MD07G1141500.v1.1 MD08G1243500.v1.1
prunus_persica	Prupe.2G162600_v2.0.a1
pyrus_communis	pycom07g14040 pycom08g21120
rosa_chinensis	RchiOBHm_Chr1g0353731
rosa_laevigata	RLG00000028263
rosa_roxburghii	Rroxscaffold_4G00301710
rosa_rugosa	Rorug01G0237200
rosa_samantha	Rh1AG249400 Rh1BG219500 Rh1CG232100 Rh1DG246500
rosa_wichuraiana	Rw1G021760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1011
AccBSI	CCGCTC	1 cut(s)	53
AciI	CCGC	9 cut(s)	5, 45, 48, 51, 94, 124, 127, 195, 623
AclI	AACGTT	1 cut(s)	576
AclWI	GGATC	2 cut(s)	509, 522
AcoI	YGGCCR	1 cut(s)	751
AcuI	CTGAAG	1 cut(s)	1017
AfaI	GTAC	3 cut(s)	76, 679, 1050
AfiI	CCNNNNNNNGG	2 cut(s)	728, 1052
AflIII	ACRYGT	1 cut(s)	71
AgsI	TTSAA	3 cut(s)	201, 449, 508
AjnI	CCWGG	2 cut(s)	922, 1051
AloI	GAACNNNNNNTCC	2 cut(s)	823, 855
AluBI	AGCT	5 cut(s)	244, 308, 700, 740, 881
AluI	AGCT	5 cut(s)	244, 308, 700, 740, 881
Alw21I	GWGCWC	1 cut(s)	489
AlwI	GGATC	2 cut(s)	509, 522
AoxI	GGCC	2 cut(s)	751, 1017
ApeKI	GCWGC	4 cut(s)	8, 690, 790, 878
AspA2I	CCTAGG	1 cut(s)	554
AspS9I	GGNCC	1 cut(s)	1064
AsuC2I	CCSGG	1 cut(s)	351
AsuHPI	GGTGA	3 cut(s)	168, 611, 913
AvaII	GGWCC	1 cut(s)	1064
AvrII	CCTAGG	1 cut(s)	554
BaeI	ACNNNNGTAYC	2 cut(s)	1032, 1065
BamHI	GGATCC	1 cut(s)	514
Bbv12I	GWGCWC	1 cut(s)	489
BbvCI	CCTCAGC	1 cut(s)	309
BbvI	GCAGC	4 cut(s)	20, 677, 777, 890
BccI	CCATC	4 cut(s)	10, 263, 503, 605
BceAI	ACGGC	1 cut(s)	95
BciT130I	CCWGG	2 cut(s)	924, 1053
BciVI	GTATCC	2 cut(s)	229, 1110
BcnI	CCSGG	1 cut(s)	351
BfaI	CTAG	2 cut(s)	555, 992
BfmI	CTRYAG	4 cut(s)	791, 807, 882, 1074
BfuI	GTATCC	2 cut(s)	229, 1110
BisI	GCNGC	9 cut(s)	6, 9, 48, 51, 127, 195, 691, 791, 879
BlnI	CCTAGG	1 cut(s)	554
BlsI	GCNGC	9 cut(s)	7, 10, 49, 52, 128, 196, 692, 792, 880
Bme1390I	CCNGG	3 cut(s)	351, 924, 1053
Bme18I	GGWCC	1 cut(s)	1064
BmgT120I	GGNCC	1 cut(s)	1064
BmiI	GGNNCC	2 cut(s)	516, 660
BmrFI	CCNGG	3 cut(s)	351, 924, 1053
BmrI	ACTGGG	1 cut(s)	1123
BmsI	GCATC	5 cut(s)	322, 457, 540, 762, 793
BmuI	ACTGGG	1 cut(s)	1123
BplI	GAGNNNNNCTC	2 cut(s)	503, 535
BpmI	CTGGAG	1 cut(s)	1029
Bpu10I	CCTNAGC	1 cut(s)	309
BpuMI	CCSGG	1 cut(s)	351
BsaAI	YACGTR	1 cut(s)	74
BsaJI	CCNNGG	2 cut(s)	471, 554
BsaXI	ACNNNNNCTCC	4 cut(s)	20, 50, 482, 512
Bsc4I	CCNNNNNNNGG	2 cut(s)	728, 1052
Bse1I	ACTGG	4 cut(s)	776, 956, 1046, 1129
Bse3DI	GCAATG	1 cut(s)	1086
BseBI	CCWGG	2 cut(s)	924, 1053
BseDI	CCNNGG	2 cut(s)	471, 554
BseGI	GGATG	9 cut(s)	132, 330, 337, 472, 531, 571, 616, 717, 777
BseLI	CCNNNNNNNGG	2 cut(s)	728, 1052
BseMI	GCAATG	1 cut(s)	1086
BseMII	CTCAG	2 cut(s)	300, 534
BseNI	ACTGG	4 cut(s)	776, 956, 1046, 1129
BseRI	GAGGAG	6 cut(s)	25, 50, 77, 80, 154, 1071
BseXI	GCAGC	4 cut(s)	20, 677, 777, 890
BsgI	GTGCAG	1 cut(s)	948
BshFI	GGCC	2 cut(s)	753, 1019
BsiHKAI	GWGCWC	1 cut(s)	489
BsiSI	CCGG	4 cut(s)	350, 461, 501, 754
BslI	CCNNNNNNNGG	2 cut(s)	728, 1052
BsnI	GGCC	2 cut(s)	753, 1019
Bsp1286I	GDGCHC	1 cut(s)	489
Bsp143I	GATC	1 cut(s)	514
BspACI	CCGC	9 cut(s)	5, 45, 48, 51, 94, 124, 127, 195, 623
BspANI	GGCC	2 cut(s)	753, 1019
BspCNI	CTCAG	2 cut(s)	301, 533
BspLI	GGNNCC	2 cut(s)	516, 660
BspMAI	CTGCAG	1 cut(s)	795
BspPI	GGATC	2 cut(s)	509, 522
BsrBI	CCGCTC	1 cut(s)	53
BsrDI	GCAATG	1 cut(s)	1086
BsrI	ACTGG	4 cut(s)	776, 956, 1046, 1129
BssECI	CCNNGG	2 cut(s)	471, 554
BssMI	GATC	1 cut(s)	514
BssT1I	CCWWGG	1 cut(s)	554
Bst2UI	CCWGG	2 cut(s)	924, 1053
Bst4CI	ACNGT	1 cut(s)	811
Bst6I	CTCTTC	2 cut(s)	172, 840
BstBAI	YACGTR	1 cut(s)	74
BstC8I	GCNNGC	2 cut(s)	55, 891
BstDEI	CTNAG	2 cut(s)	309, 520
BstDSI	CCRYGG	1 cut(s)	471
BstENI	CCTNNNNNAGG	1 cut(s)	726
BstF5I	GGATG	9 cut(s)	132, 330, 337, 472, 531, 571, 616, 717, 777
BstKTI	GATC	1 cut(s)	517
BstMBI	GATC	1 cut(s)	514
BstNI	CCWGG	2 cut(s)	924, 1053
BstSCI	CCNGG	3 cut(s)	349, 922, 1051
BstSFI	CTRYAG	4 cut(s)	791, 807, 882, 1074
BstV1I	GCAGC	4 cut(s)	20, 677, 777, 890
BstX2I	RGATCY	1 cut(s)	514
BstYI	RGATCY	1 cut(s)	514
BsuI	GTATCC	2 cut(s)	229, 1110
BsuRI	GGCC	2 cut(s)	753, 1019
BtgI	CCRYGG	1 cut(s)	471
BtsCI	GGATG	9 cut(s)	132, 330, 337, 472, 531, 571, 616, 717, 777
Cac8I	GCNNGC	2 cut(s)	55, 891
Cfr13I	GGNCC	1 cut(s)	1064
CsiI	ACCWGGT	1 cut(s)	922
Csp6I	GTAC	3 cut(s)	75, 678, 1049
CviAII	CATG	2 cut(s)	527, 646
CviQI	GTAC	3 cut(s)	75, 678, 1049
DdeI	CTNAG	2 cut(s)	309, 520
DpnI	GATC	1 cut(s)	516
DpnII	GATC	1 cut(s)	514
DraI	TTTAAA	1 cut(s)	582
EaeI	YGGCCR	1 cut(s)	751
Eam1104I	CTCTTC	2 cut(s)	172, 840
EarI	CTCTTC	2 cut(s)	172, 840
Eco130I	CCWWGG	1 cut(s)	554
Eco47I	GGWCC	1 cut(s)	1064
Eco57I	CTGAAG	1 cut(s)	1017
EcoNI	CCTNNNNNAGG	1 cut(s)	726
EcoRII	CCWGG	2 cut(s)	922, 1051
EcoT14I	CCWWGG	1 cut(s)	554
ErhI	CCWWGG	1 cut(s)	554
FaeI	CATG	2 cut(s)	530, 649
FatI	CATG	2 cut(s)	526, 645
FauI	CCCGC	2 cut(s)	131, 630
FauNDI	CATATG	1 cut(s)	561
Fnu4HI	GCNGC	9 cut(s)	6, 9, 48, 51, 127, 195, 691, 791, 879
FokI	GGATG	9 cut(s)	119, 337, 344, 479, 518, 578, 623, 704, 784
Fsp4HI	GCNGC	9 cut(s)	6, 9, 48, 51, 127, 195, 691, 791, 879
FspBI	CTAG	2 cut(s)	555, 992
GluI	GCNGC	9 cut(s)	6, 9, 48, 51, 127, 195, 691, 791, 879
GsuI	CTGGAG	1 cut(s)	1029
HaeIII	GGCC	2 cut(s)	753, 1019
HapII	CCGG	4 cut(s)	350, 461, 501, 754
Hin1II	CATG	2 cut(s)	530, 649
HincII	GTYRAC	1 cut(s)	852
HindII	GTYRAC	1 cut(s)	852
HinfI	GANTC	4 cut(s)	386, 491, 838, 948
HpaI	GTTAAC	1 cut(s)	852
HpaII	CCGG	4 cut(s)	350, 461, 501, 754
HphI	GGTGA	3 cut(s)	168, 611, 913
Hpy166II	GTNNAC	1 cut(s)	852
Hpy188I	TCNGA	2 cut(s)	235, 947
Hpy188III	TCNNGA	2 cut(s)	828, 842
Hpy8I	GTNNAC	1 cut(s)	852
Hpy99I	CGWCG	2 cut(s)	16, 116
HpyAV	CCTTC	4 cut(s)	76, 322, 406, 673
HpyCH4III	ACNGT	1 cut(s)	811
HpyCH4IV	ACGT	2 cut(s)	73, 576
HpyCH4V	TGCA	6 cut(s)	335, 454, 693, 706, 793, 929
HpyF3I	CTNAG	2 cut(s)	309, 520
HpySE526I	ACGT	2 cut(s)	73, 576
Hsp92II	CATG	2 cut(s)	530, 649
KspAI	GTTAAC	1 cut(s)	852
Kzo9I	GATC	1 cut(s)	514
LmnI	GCTCC	2 cut(s)	38, 756
Lsp1109I	GCAGC	4 cut(s)	20, 677, 777, 890
LweI	GCATC	5 cut(s)	322, 457, 540, 762, 793
MabI	ACCWGGT	1 cut(s)	922
MaeI	CTAG	2 cut(s)	555, 992
MaeII	ACGT	2 cut(s)	73, 576
MaeIII	GTNAC	1 cut(s)	543
MalI	GATC	1 cut(s)	516
MbiI	CCGCTC	1 cut(s)	53
MboI	GATC	1 cut(s)	514
MboII	GAAGA	6 cut(s)	159, 286, 395, 421, 812, 857
MfeI	CAATTG	3 cut(s)	185, 201, 336
MflI	RGATCY	1 cut(s)	514
MhlI	GDGCHC	1 cut(s)	489
MluCI	AATT	7 cut(s)	185, 201, 291, 336, 747, 1026, 1038
MlyI	GAGTC	1 cut(s)	485
MmeI	TCCRAC	4 cut(s)	352, 893, 925, 976
MseI	TTAA	3 cut(s)	581, 851, 918
MslI	CAYNNNNRTG	2 cut(s)	564, 711
MspA1I	CMGCKG	1 cut(s)	881
MspI	CCGG	4 cut(s)	350, 461, 501, 754
MspR9I	CCNGG	3 cut(s)	351, 924, 1053
MunI	CAATTG	3 cut(s)	185, 201, 336
MvaI	CCWGG	2 cut(s)	924, 1053
NciI	CCSGG	1 cut(s)	351
NdeI	CATATG	1 cut(s)	561
NdeII	GATC	1 cut(s)	514
NlaIII	CATG	2 cut(s)	530, 649
NlaIV	GGNNCC	2 cut(s)	516, 660
PfeI	GAWTC	3 cut(s)	386, 838, 948
PkrI	GCNGC	9 cut(s)	7, 10, 49, 52, 128, 196, 692, 792, 880
PleI	GAGTC	1 cut(s)	485
PpsI	GAGTC	1 cut(s)	485
Ppu21I	YACGTR	1 cut(s)	74
PsiI	TTATAA	1 cut(s)	1011
Psp1406I	AACGTT	1 cut(s)	576
Psp6I	CCWGG	2 cut(s)	922, 1051
PspGI	CCWGG	2 cut(s)	922, 1051
PspN4I	GGNNCC	2 cut(s)	516, 660
PspPI	GGNCC	1 cut(s)	1064
PstI	CTGCAG	1 cut(s)	795
PsuI	RGATCY	1 cut(s)	514
PvuII	CAGCTG	1 cut(s)	881
RsaI	GTAC	3 cut(s)	76, 679, 1050
RsaNI	GTAC	3 cut(s)	75, 678, 1049
RseI	CAYNNNNRTG	2 cut(s)	564, 711
SaqAI	TTAA	3 cut(s)	581, 851, 918
SatI	GCNGC	9 cut(s)	6, 9, 48, 51, 127, 195, 691, 791, 879
Sau3AI	GATC	1 cut(s)	514
Sau96I	GGNCC	1 cut(s)	1064
SchI	GAGTC	1 cut(s)	485
ScrFI	CCNGG	3 cut(s)	351, 924, 1053
SduI	GDGCHC	1 cut(s)	489
SexAI	ACCWGGT	1 cut(s)	922
SfaNI	GCATC	5 cut(s)	322, 457, 540, 762, 793
SfcI	CTRYAG	4 cut(s)	791, 807, 882, 1074
SinI	GGWCC	1 cut(s)	1064
SmiMI	CAYNNNNRTG	2 cut(s)	564, 711
Sse9I	AATT	7 cut(s)	185, 201, 291, 336, 747, 1026, 1038
SsiI	CCGC	9 cut(s)	5, 45, 48, 51, 94, 124, 127, 195, 623
SspI	AATATT	2 cut(s)	631, 763
SspMI	CTAG	2 cut(s)	555, 992
StyD4I	CCNGG	3 cut(s)	349, 922, 1051
StyI	CCWWGG	1 cut(s)	554
TaaI	ACNGT	1 cut(s)	811
TaiI	ACGT	2 cut(s)	76, 579
TaqI	TCGA	2 cut(s)	14, 489
TasI	AATT	7 cut(s)	185, 201, 291, 336, 747, 1026, 1038
TatI	WGTACW	1 cut(s)	677
TauI	GCSGC	5 cut(s)	8, 50, 53, 129, 197
TfiI	GAWTC	3 cut(s)	386, 838, 948
Tru1I	TTAA	3 cut(s)	581, 851, 918
Tru9I	TTAA	3 cut(s)	581, 851, 918
TseI	GCWGC	4 cut(s)	8, 690, 790, 878
TspDTI	ATGAA	3 cut(s)	341, 515, 582
TspGWI	ACGGA	2 cut(s)	41, 488
VpaK11BI	GGWCC	1 cut(s)	1064
XagI	CCTNNNNNAGG	1 cut(s)	726
XmaJI	CCTAGG	1 cut(s)	554
XspI	CTAG	2 cut(s)	555, 992

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.