Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0013163

Physical Location & Seq

Genomic Coordinates Forward (+)

20521686 .. 20524402

Gene Structure 2717 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1141500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGGCTTCGATGGTATTCACGGACTGCTGCTCCACCACCACGCCTCGCCTCCTCCCCTTTCGGCGGAACCCCCTCTTCCACCGCAACCACGGCGCCGTTTTCCTTCGCCCCCTCATAAATCCTAGCTCCCCTAGACGCCTCTCCCTTCACTCCTCCTCCGCTTCTGCTTCGCCGTCTCCGTCGCCCACCGCTTCATTGGCCACTGAAGAATCGGCAAAAGTGATCGATGGGAAAGCCGTTGCGAAGCAAATCAGAGATGAGATCAATGCAGAAGTGTCGAAGATGAAGGATGCAATTGGCATTGTTCCGGGTTTGGCAGTTATTCTTGTTGGGGACAGGAAGGACTCTGCCACTTATGTGCGCAACAAGAAGAAAGCTTGTGAGTCCGTGGGGATTAATTCTTTTGAAGTGCGCTTGCCCGAGGGTTCCCCGGAACAAGAAGTGCTCAACTCCATTGCGGGATTCAATGATGATCCTTCAGTTCATGGCATCCTTGTGCAGCTACCCCTGCCTTGTCATATGAATGAGCAAAACATCTTAAATGCTGTTAGCATTGAGAAGGACGTGGATGGGTTTCACCCGCTAAATATTGGCCGTCTTGCCATGCGTGGTAGGGACCCCTTGTTTGTTCCTTGCACGCCAAAAGGATGCATAGAGTTGTTGCATAGATATGGCATCCCTATCAAGGGAAAGAGAGCTGTTGTAATTGGCCGGAGCAATATTGTTGGGATGCCAGCTGCTTTATTGTTGCAGAGGGAAGATGCTACAATCAGTATAGTCCATTCCCGAACCAAGAATCCTGAGGAGATCACAAGACAAGCGGATATTATAATCTCTGCCGTGGGGCAACCAAATATGGTGAGGGGCAGCTGGATCAAACCCGGTGCAGTAATTATTGATGTCGGAATCAATCCAGTTGAGGATGAAAAGAGTCCTCGAGGTTATCGGCTGGTTGGAGATGTGTGTTACGAGGAGGCCAGCCAGATTGCTTCAGCAATTACTCCAGTTCCCGGGGGAGTGGGTCCAATGACAATAGCAATGCTTCTCTCTAATACTCTCGTATCAGCAAAAAGAATACACAGCTTCCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004477 GO:0004488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006730 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0019238 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

364

Amino Acids

39.15

Weight (kDa)

8.95

Isoelectric Point (pI)

51.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THF_DHG_CYH	PF00763	75 - 190	3.7e-43	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C	PF02882	211 - 358	1.9e-61	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013163)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00600 AT4G00620
fragaria_vesca	FvH4_7g13650 FvH4_7g13650
malus_domestica	MD07G1141500.v1.1 MD08G1243500.v1.1
prunus_persica	Prupe.2G162600_v2.0.a1
pyrus_communis	pycom07g14040 pycom08g21120
rosa_chinensis	RchiOBHm_Chr1g0353731
rosa_laevigata	RLG00000028263
rosa_roxburghii	Rroxscaffold_4G00301710
rosa_rugosa	Rorug01G0237200
rosa_samantha	Rh1AG249400 Rh1BG219500 Rh1CG232100 Rh1DG246500
rosa_wichuraiana	Rw1G021760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	830
AbsI	CCTCGAGG	1 cut(s)	936
Acc16I	TGCGCA	1 cut(s)	362
AccB1I	GGYRCC	1 cut(s)	92
AciI	CCGC	7 cut(s)	64, 82, 159, 189, 458, 581, 821
AclWI	GGATC	2 cut(s)	467, 881
AcoI	YGGCCR	3 cut(s)	198, 592, 709
AcuI	CTGAAG	3 cut(s)	225, 462, 975
AcyI	GRCGYC	2 cut(s)	93, 136
AfiI	CCNNNNNNNGG	4 cut(s)	63, 685, 686, 1010
AgsI	TTSAA	2 cut(s)	407, 466
AjiI	CACGTC	1 cut(s)	565
AleI	CACNNNNGTG	1 cut(s)	356
AloI	GAACNNNNNNTCC	2 cut(s)	781, 813
AluBI	AGCT	7 cut(s)	126, 377, 502, 698, 737, 870, 1083
AluI	AGCT	7 cut(s)	126, 377, 502, 698, 737, 870, 1083
Alw21I	GWGCWC	1 cut(s)	447
Alw26I	GTCTC	1 cut(s)	180
AlwI	GGATC	2 cut(s)	467, 881
Ama87I	CYCGRG	3 cut(s)	419, 936, 1010
AoxI	GGCC	4 cut(s)	198, 592, 709, 975
ApeKI	GCWGC	4 cut(s)	27, 499, 737, 867
ArsI	GACNNNNNNTTYG	2 cut(s)	608, 640
AseI	ATTAAT	1 cut(s)	396
AspLEI	GCGC	3 cut(s)	95, 363, 414
AspS9I	GGNCC	2 cut(s)	616, 1022
AsuC2I	CCSGG	5 cut(s)	309, 431, 882, 1011, 1012
AsuHPI	GGTGA	2 cut(s)	569, 871
AvaI	CYCGRG	3 cut(s)	419, 936, 1010
AvaII	GGWCC	2 cut(s)	616, 1022
AxyI	CCTNAGG	1 cut(s)	801
BaeI	ACNNNNGTAYC	2 cut(s)	1044, 1077
BalI	TGGCCA	1 cut(s)	200
BanI	GGYRCC	1 cut(s)	92
Bbv12I	GWGCWC	1 cut(s)	447
BbvI	GCAGC	4 cut(s)	14, 511, 724, 879
BccI	CCATC	3 cut(s)	4, 221, 563
BceAI	ACGGC	6 cut(s)	80, 106, 157, 221, 579, 824
BcgI	CGANNNNNNTGC	2 cut(s)	258, 292
BcnI	CCSGG	5 cut(s)	309, 431, 882, 1011, 1012
BcoDI	GTCTC	1 cut(s)	180
BfaI	CTAG	2 cut(s)	123, 132
BfoI	RGCGCY	1 cut(s)	96
BisI	GCNGC	4 cut(s)	28, 500, 738, 868
BlsI	GCNGC	4 cut(s)	29, 501, 739, 869
Bme1390I	CCNGG	5 cut(s)	309, 431, 882, 1011, 1012
Bme18I	GGWCC	2 cut(s)	616, 1022
BmeT110I	CYCGRG	3 cut(s)	419, 936, 1010
BmgBI	CACGTC	1 cut(s)	565
BmgT120I	GGNCC	2 cut(s)	616, 1022
BmiI	GGNNCC	6 cut(s)	68, 94, 427, 617, 618, 1023
BmrFI	CCNGG	5 cut(s)	309, 431, 882, 1011, 1012
BmsI	GCATC	6 cut(s)	280, 498, 638, 684, 720, 751
BpmI	CTGGAG	1 cut(s)	987
BpuMI	CCSGG	5 cut(s)	309, 431, 882, 1011, 1012
Bsa29I	ATCGAT	1 cut(s)	225
BsaHI	GRCGYC	2 cut(s)	93, 136
BsaJI	CCNNGG	7 cut(s)	88, 387, 420, 429, 840, 1010, 1011
BsaXI	ACNNNNNCTCC	4 cut(s)	14, 44, 140, 170
Bsc4I	CCNNNNNNNGG	4 cut(s)	63, 685, 686, 1010
Bse1I	ACTGG	3 cut(s)	914, 1004, 1087
Bse21I	CCTNAGG	1 cut(s)	801
Bse3DI	GCAATG	2 cut(s)	453, 1044
BseCI	ATCGAT	1 cut(s)	225
BseDI	CCNNGG	7 cut(s)	88, 387, 420, 429, 840, 1010, 1011
BseGI	GGATG	7 cut(s)	295, 489, 574, 653, 675, 735, 928
BseLI	CCNNNNNNNGG	4 cut(s)	63, 685, 686, 1010
BseMI	GCAATG	2 cut(s)	453, 1044
BseMII	CTCAG	1 cut(s)	792
BseNI	ACTGG	3 cut(s)	914, 1004, 1087
BseRI	GAGGAG	5 cut(s)	41, 142, 145, 818, 986
BseXI	GCAGC	4 cut(s)	14, 511, 724, 879
BsgI	GTGCAG	2 cut(s)	518, 906
BshFI	GGCC	4 cut(s)	200, 594, 711, 977
BshNI	GGYRCC	1 cut(s)	92
BshVI	ATCGAT	1 cut(s)	225
BsiHKAI	GWGCWC	1 cut(s)	447
BsiHKCI	CYCGRG	3 cut(s)	419, 936, 1010
BsiSI	CCGG	5 cut(s)	308, 431, 712, 882, 1011
BslFI	GGGAC	2 cut(s)	347, 629
BslI	CCNNNNNNNGG	4 cut(s)	63, 685, 686, 1010
BsmAI	GTCTC	1 cut(s)	180
BsmBI	CGTCTC	1 cut(s)	180
BsmFI	GGGAC	2 cut(s)	347, 629
BsnI	GGCC	4 cut(s)	200, 594, 711, 977
BsoBI	CYCGRG	3 cut(s)	419, 936, 1010
Bsp1286I	GDGCHC	1 cut(s)	447
Bsp143I	GATC	5 cut(s)	222, 261, 472, 807, 873
BspACI	CCGC	7 cut(s)	64, 82, 159, 189, 458, 581, 821
BspANI	GGCC	4 cut(s)	200, 594, 711, 977
BspCNI	CTCAG	1 cut(s)	793
BspDI	ATCGAT	1 cut(s)	225
BspLI	GGNNCC	6 cut(s)	68, 94, 427, 617, 618, 1023
BspPI	GGATC	2 cut(s)	467, 881
BspT107I	GGYRCC	1 cut(s)	92
BsrDI	GCAATG	2 cut(s)	453, 1044
BsrI	ACTGG	3 cut(s)	914, 1004, 1087
BssECI	CCNNGG	7 cut(s)	88, 387, 420, 429, 840, 1010, 1011
BssMI	GATC	5 cut(s)	222, 261, 472, 807, 873
BssNI	GRCGYC	2 cut(s)	93, 136
Bst6I	CTCTTC	1 cut(s)	80
BstACI	GRCGYC	2 cut(s)	93, 136
BstC8I	GCNNGC	4 cut(s)	416, 638, 735, 979
BstDEI	CTNAG	1 cut(s)	801
BstDSI	CCRYGG	3 cut(s)	88, 387, 840
BstF5I	GGATG	7 cut(s)	295, 489, 574, 653, 675, 735, 928
BstH2I	RGCGCY	1 cut(s)	96
BstHHI	GCGC	3 cut(s)	95, 363, 414
BstKTI	GATC	5 cut(s)	225, 264, 475, 810, 876
BstMAI	GTCTC	1 cut(s)	180
BstMBI	GATC	5 cut(s)	222, 261, 472, 807, 873
BstMWI	GCNNNNNNNGC	3 cut(s)	90, 197, 508
BstSCI	CCNGG	5 cut(s)	307, 429, 880, 1009, 1010
BstV1I	GCAGC	4 cut(s)	14, 511, 724, 879
Bsu15I	ATCGAT	1 cut(s)	225
Bsu36I	CCTNAGG	1 cut(s)	801
BsuRI	GGCC	4 cut(s)	200, 594, 711, 977
BsuTUI	ATCGAT	1 cut(s)	225
BtgI	CCRYGG	3 cut(s)	88, 387, 840
BtrI	CACGTC	1 cut(s)	565
BtsCI	GGATG	7 cut(s)	295, 489, 574, 653, 675, 735, 928
BtsIMutI	CAGTG	1 cut(s)	201
Cac8I	GCNNGC	4 cut(s)	416, 638, 735, 979
CfoI	GCGC	3 cut(s)	95, 363, 414
Cfr13I	GGNCC	2 cut(s)	616, 1022
Cfr9I	CCCGGG	1 cut(s)	1010
ClaI	ATCGAT	1 cut(s)	225
CseI	GACGC	1 cut(s)	144
CviAII	CATG	2 cut(s)	485, 604
DdeI	CTNAG	1 cut(s)	801
DinI	GGCGCC	1 cut(s)	94
DpnI	GATC	5 cut(s)	224, 263, 474, 809, 875
DpnII	GATC	5 cut(s)	222, 261, 472, 807, 873
EaeI	YGGCCR	3 cut(s)	198, 592, 709
Eam1104I	CTCTTC	1 cut(s)	80
EarI	CTCTTC	1 cut(s)	80
EciI	GGCGGA	1 cut(s)	79
Eco47I	GGWCC	2 cut(s)	616, 1022
Eco57I	CTGAAG	3 cut(s)	225, 462, 975
Eco81I	CCTNAGG	1 cut(s)	801
Eco88I	CYCGRG	3 cut(s)	419, 936, 1010
EcoO109I	RGGNCCY	1 cut(s)	616
EcoT22I	ATGCAT	1 cut(s)	653
EgeI	GGCGCC	1 cut(s)	94
EheI	GGCGCC	1 cut(s)	94
Esp3I	CGTCTC	1 cut(s)	180
FaeI	CATG	2 cut(s)	488, 607
FaqI	GGGAC	2 cut(s)	347, 629
FatI	CATG	2 cut(s)	484, 603
FauI	CCCGC	2 cut(s)	451, 588
FauNDI	CATATG	1 cut(s)	519
Fnu4HI	GCNGC	4 cut(s)	28, 500, 738, 868
FokI	GGATG	7 cut(s)	302, 476, 581, 660, 662, 742, 935
Fsp4HI	GCNGC	4 cut(s)	28, 500, 738, 868
FspBI	CTAG	2 cut(s)	123, 132
FspI	TGCGCA	1 cut(s)	362
GlaI	GCGC	3 cut(s)	94, 362, 413
GluI	GCNGC	4 cut(s)	28, 500, 738, 868
GsuI	CTGGAG	1 cut(s)	987
HaeII	RGCGCY	1 cut(s)	96
HaeIII	GGCC	4 cut(s)	200, 594, 711, 977
HapII	CCGG	5 cut(s)	308, 431, 712, 882, 1011
HgaI	GACGC	1 cut(s)	144
HhaI	GCGC	3 cut(s)	95, 363, 414
Hin1I	GRCGYC	2 cut(s)	93, 136
Hin1II	CATG	2 cut(s)	488, 607
Hin6I	GCGC	3 cut(s)	93, 361, 412
HinP1I	GCGC	3 cut(s)	93, 361, 412
HindIII	AAGCTT	1 cut(s)	375
HinfI	GANTC	7 cut(s)	209, 344, 383, 462, 796, 906, 931
HpaII	CCGG	5 cut(s)	308, 431, 712, 882, 1011
HphI	GGTGA	2 cut(s)	569, 871
Hpy188I	TCNGA	2 cut(s)	254, 905
Hpy188III	TCNNGA	2 cut(s)	786, 800
Hpy99I	CGWCG	1 cut(s)	184
HpyAV	CCTTC	6 cut(s)	113, 155, 280, 334, 486, 553
HpyCH4IV	ACGT	1 cut(s)	564
HpyCH4V	TGCA	8 cut(s)	269, 293, 499, 636, 651, 664, 751, 887
HpyF10VI	GCNNNNNNNGC	3 cut(s)	90, 197, 508
HpyF3I	CTNAG	1 cut(s)	801
HpySE526I	ACGT	1 cut(s)	564
Hsp92I	GRCGYC	2 cut(s)	93, 136
Hsp92II	CATG	2 cut(s)	488, 607
HspAI	GCGC	3 cut(s)	93, 361, 412
KasI	GGCGCC	1 cut(s)	92
KflI	GGGWCCC	1 cut(s)	616
Kzo9I	GATC	5 cut(s)	222, 261, 472, 807, 873
LmnI	GCTCC	3 cut(s)	35, 131, 714
Lsp1109I	GCAGC	4 cut(s)	14, 511, 724, 879
LweI	GCATC	6 cut(s)	280, 498, 638, 684, 720, 751
MaeI	CTAG	2 cut(s)	123, 132
MaeII	ACGT	1 cut(s)	564
MaeIII	GTNAC	1 cut(s)	965
MalI	GATC	5 cut(s)	224, 263, 474, 809, 875
MboI	GATC	5 cut(s)	222, 261, 472, 807, 873
MboII	GAAGA	5 cut(s)	67, 218, 292, 382, 770
MfeI	CAATTG	1 cut(s)	294
MhlI	GDGCHC	1 cut(s)	447
MlsI	TGGCCA	1 cut(s)	200
MluCI	AATT	5 cut(s)	294, 397, 705, 891, 996
MluNI	TGGCCA	1 cut(s)	200
Mly113I	GGCGCC	1 cut(s)	93
MlyI	GAGTC	3 cut(s)	338, 392, 940
MmeI	TCCRAC	2 cut(s)	883, 934
Mox20I	TGGCCA	1 cut(s)	200
Mph1103I	ATGCAT	1 cut(s)	653
MscI	TGGCCA	1 cut(s)	200
MseI	TTAA	2 cut(s)	396, 539
MslI	CAYNNNNRTG	4 cut(s)	356, 494, 522, 669
Msp20I	TGGCCA	1 cut(s)	200
MspA1I	CMGCKG	2 cut(s)	737, 870
MspI	CCGG	5 cut(s)	308, 431, 712, 882, 1011
MspR9I	CCNGG	5 cut(s)	309, 431, 882, 1011, 1012
MunI	CAATTG	1 cut(s)	294
MwoI	GCNNNNNNNGC	3 cut(s)	90, 197, 508
NarI	GGCGCC	1 cut(s)	93
NciI	CCSGG	5 cut(s)	309, 431, 882, 1011, 1012
NdeI	CATATG	1 cut(s)	519
NdeII	GATC	5 cut(s)	222, 261, 472, 807, 873
NlaIII	CATG	2 cut(s)	488, 607
NlaIV	GGNNCC	6 cut(s)	68, 94, 427, 617, 618, 1023
NsbI	TGCGCA	1 cut(s)	362
NsiI	ATGCAT	1 cut(s)	653
OliI	CACNNNNGTG	1 cut(s)	356
PaeR7I	CTCGAG	1 cut(s)	936
PcsI	WCGNNNNNNNCGW	1 cut(s)	176
PfeI	GAWTC	4 cut(s)	209, 462, 796, 906
PkrI	GCNGC	4 cut(s)	29, 501, 739, 869
PleI	GAGTC	3 cut(s)	338, 391, 939
PluTI	GGCGCC	1 cut(s)	96
PpsI	GAGTC	3 cut(s)	338, 391, 939
PpuMI	RGGWCCY	1 cut(s)	616
PshBI	ATTAAT	1 cut(s)	396
PsiI	TTATAA	1 cut(s)	830
Psp5II	RGGWCCY	1 cut(s)	616
PspN4I	GGNNCC	6 cut(s)	68, 94, 427, 617, 618, 1023
PspPI	GGNCC	2 cut(s)	616, 1022
PspPPI	RGGWCCY	1 cut(s)	616
PspXI	VCTCGAGB	1 cut(s)	936
PvuII	CAGCTG	2 cut(s)	737, 870
RseI	CAYNNNNRTG	4 cut(s)	356, 494, 522, 669
SaqAI	TTAA	2 cut(s)	396, 539
SatI	GCNGC	4 cut(s)	28, 500, 738, 868
Sau3AI	GATC	5 cut(s)	222, 261, 472, 807, 873
Sau96I	GGNCC	2 cut(s)	616, 1022
SchI	GAGTC	3 cut(s)	338, 392, 940
ScrFI	CCNGG	5 cut(s)	309, 431, 882, 1011, 1012
SduI	GDGCHC	1 cut(s)	447
SetI	ASST	9 cut(s)	128, 379, 504, 567, 700, 739, 872, 943, 1085
SfaNI	GCATC	6 cut(s)	280, 498, 638, 684, 720, 751
SfoI	GGCGCC	1 cut(s)	94
Sfr274I	CTCGAG	1 cut(s)	936
SinI	GGWCC	2 cut(s)	616, 1022
SlaI	CTCGAG	1 cut(s)	936
SmaI	CCCGGG	1 cut(s)	1012
SmiMI	CAYNNNNRTG	4 cut(s)	356, 494, 522, 669
SmlI	CTYRAG	1 cut(s)	936
SmoI	CTYRAG	1 cut(s)	936
Sse9I	AATT	5 cut(s)	294, 397, 705, 891, 996
SsiI	CCGC	7 cut(s)	64, 82, 159, 189, 458, 581, 821
SspDI	GGCGCC	1 cut(s)	92
SspI	AATATT	2 cut(s)	589, 721
SspMI	CTAG	2 cut(s)	123, 132
StyD4I	CCNGG	5 cut(s)	307, 429, 880, 1009, 1010
TaiI	ACGT	1 cut(s)	567
TaqI	TCGA	4 cut(s)	8, 225, 278, 937
TasI	AATT	5 cut(s)	294, 397, 705, 891, 996
TfiI	GAWTC	4 cut(s)	209, 462, 796, 906
Tru1I	TTAA	2 cut(s)	396, 539
Tru9I	TTAA	2 cut(s)	396, 539
TscAI	CASTG	1 cut(s)	208
TseI	GCWGC	4 cut(s)	27, 499, 737, 867
TspDTI	ATGAA	5 cut(s)	183, 299, 473, 536, 939
TspGWI	ACGGA	3 cut(s)	35, 168, 376
TspMI	CCCGGG	1 cut(s)	1010
TspRI	CASTG	1 cut(s)	208
VpaK11BI	GGWCC	2 cut(s)	616, 1022
VspI	ATTAAT	1 cut(s)	396
XcmI	CCANNNNNNNNNTGG	1 cut(s)	193
XhoI	CTCGAG	1 cut(s)	936
XmaI	CCCGGG	1 cut(s)	1010
XspI	CTAG	2 cut(s)	123, 132
Zsp2I	ATGCAT	1 cut(s)	653

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.