pycom07g14040

Bifunctional protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0013163
Physical Location & Seq
Forward (+)
15875879 .. 15878284
2406 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g14040.3

Sequence Viewer

Length: 1092 bp
ATGGCTTCGATGCTATTCACGGACTGCTCCTCCACCACCACGCCTCGCCTCCTCCCCTTTCGGCGGAACCCCCTCTTCCACCGCAACCACGGCGCCGTTTTCCTTCGCCCCCTCGTAAATCCTAGCTCCCCTAGACGCCTCCCCCTGCACTCCTCCTCCGCTTCTTCTTCGCTGTCTCCGTCGCCCACCGTTTCCTTGGCCACTGAAGCATCGGCAAAAGTGATCGATGGGAAAGTCATTGCGAAGCAAATCAGAGATGAGATCAATGCAGAAGTGTCGAAGATGAAGGATGCAATTGGCATTGTTCCGGGTTTGGCAGTTATTCTTGTTGGGGACAGGAAGGACTCTGCCACTTATGTGCGCAACAAGAAGAAAGCTTGTGAGTCCGTGGGTATTAATTCTTTCGAAGTGCGCTTGCCTGAGGATTCCCCGGAACAAGAAGTGCTCAACTCCATCGCGGGGTTCAATGATGATCCTTCAGTTCATGGCATCCTTGTGCAGCTACCCCTGCCTTCTCATATGAATGAGCAAAACATCTTAAATGCTGTTAGCATTGAGAAAGACGTGGATGGGTTTCACCCGCTAAATATTGGCCGTCTTGCCATGCGTGGTAGGGACCCCTTGTTTGTTCCTTGCACGCCAAAAGGATGCATAGAGTTGTTGCATAGATATGGCATCCCTATCAAGGGAAAGAGAGCTGTTGTAATTGGCCGGAGCAATATTGTTGGGATGCCAGCTGCTTTATTGTTGCAGAGGGAAGATGCTACAATCAGCATAGTCCATTCCCGAACCAAGAATCCTGAGGAGATCACAAGACAAGCGGATATTATAATCTCTGCTGTGGGGCAACCAAATATGGTGAGGGGCAGCTCGATCAAACCTGGTGCAGTAATTATTGATGTCGGAATCAATCCAGTTGAGGATGAAAAGAATCCTCGAGGTTATCGGCTGGTTGGAGATGTGTGTTACGAGGAGGCCAGCCAGATTGCTTCAGCAATTACTCCAGTTCCCGGGGGAGTGGGTCCAATGACAATAGCAATGCTTCTCTCCAATACTCTCGTATCAGCAAAAAGAATACACAACTTCCAGTAA

Protein Analysis

364

Amino Acids

39.18

Weight (kDa)

9.08

Isoelectric Point (pI)

50.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00600 AT4G00620
fragaria_vesca FvH4_7g13650 FvH4_7g13650
malus_domestica MD07G1141500.v1.1 MD08G1243500.v1.1
prunus_persica Prupe.2G162600_v2.0.a1
pyrus_communis pycom07g14040 pycom08g21120
rosa_chinensis RchiOBHm_Chr1g0353731
rosa_laevigata RLG00000028263
rosa_roxburghii Rroxscaffold_4G00301710
rosa_rugosa Rorug01G0237200
rosa_samantha Rh1AG249400 Rh1BG219500 Rh1CG232100 Rh1DG246500
rosa_wichuraiana Rw1G021760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AbsI CCTCGAGG 1 cut(s) 936
Acc16I TGCGCA 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 92
AccII CGCG 1 cut(s) 458
AciI CCGC 6 cut(s) 64, 82, 159, 458, 581, 821
AclWI GGATC 1 cut(s) 467
AcoI YGGCCR 3 cut(s) 198, 592, 709
AcuI CTGAAG 3 cut(s) 225, 462, 975
AcyI GRCGYC 2 cut(s) 93, 136
AfiI CCNNNNNNNGG 5 cut(s) 63, 459, 685, 686, 1010
AgsI TTSAA 1 cut(s) 466
AjiI CACGTC 1 cut(s) 565
AjnI CCWGG 1 cut(s) 880
AleI CACNNNNGTG 1 cut(s) 356
AloI GAACNNNNNNTCC 2 cut(s) 781, 813
AluBI AGCT 6 cut(s) 126, 377, 502, 698, 737, 870
AluI AGCT 6 cut(s) 126, 377, 502, 698, 737, 870
Alw21I GWGCWC 1 cut(s) 447
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 1 cut(s) 467
Ama87I CYCGRG 2 cut(s) 936, 1010
AoxI GGCC 4 cut(s) 198, 592, 709, 975
ApeKI GCWGC 3 cut(s) 499, 737, 867
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
AseI ATTAAT 1 cut(s) 396
AspLEI GCGC 3 cut(s) 95, 363, 414
AspS9I GGNCC 2 cut(s) 616, 1022
AsuC2I CCSGG 4 cut(s) 309, 431, 1011, 1012
AsuHPI GGTGA 2 cut(s) 569, 871
AsuII TTCGAA 1 cut(s) 405
AvaI CYCGRG 2 cut(s) 936, 1010
AvaII GGWCC 2 cut(s) 616, 1022
AxyI CCTNAGG 2 cut(s) 420, 801
BaeI ACNNNNGTAYC 2 cut(s) 1044, 1077
BalI TGGCCA 1 cut(s) 200
BanI GGYRCC 1 cut(s) 92
Bbv12I GWGCWC 1 cut(s) 447
BbvI GCAGC 3 cut(s) 511, 724, 879
BccI CCATC 3 cut(s) 221, 461, 563
BceAI ACGGC 3 cut(s) 80, 106, 579
BcgI CGANNNNNNTGC 2 cut(s) 258, 292
BciT130I CCWGG 1 cut(s) 882
BcnI CCSGG 4 cut(s) 309, 431, 1011, 1012
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 2 cut(s) 123, 132
BfoI RGCGCY 1 cut(s) 96
BisI GCNGC 3 cut(s) 500, 738, 868
BlsI GCNGC 3 cut(s) 501, 739, 869
Bme1390I CCNGG 5 cut(s) 309, 431, 882, 1011, 1012
Bme18I GGWCC 2 cut(s) 616, 1022
BmeT110I CYCGRG 2 cut(s) 936, 1010
BmgBI CACGTC 1 cut(s) 565
BmgT120I GGNCC 2 cut(s) 616, 1022
BmiI GGNNCC 5 cut(s) 68, 94, 617, 618, 1023
BmrFI CCNGG 5 cut(s) 309, 431, 882, 1011, 1012
BmsI GCATC 7 cut(s) 218, 280, 498, 638, 684, 720, 751
BpmI CTGGAG 1 cut(s) 987
Bpu14I TTCGAA 1 cut(s) 405
BpuMI CCSGG 4 cut(s) 309, 431, 1011, 1012
Bsa29I ATCGAT 1 cut(s) 225
BsaHI GRCGYC 2 cut(s) 93, 136
BsaJI CCNNGG 6 cut(s) 88, 195, 387, 429, 1010, 1011
BsaXI ACNNNNNCTCC 4 cut(s) 14, 44, 140, 170
Bsc4I CCNNNNNNNGG 5 cut(s) 63, 459, 685, 686, 1010
Bse1I ACTGG 3 cut(s) 914, 1004, 1087
Bse21I CCTNAGG 2 cut(s) 420, 801
Bse3DI GCAATG 2 cut(s) 237, 1044
BseBI CCWGG 1 cut(s) 882
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 6 cut(s) 88, 195, 387, 429, 1010, 1011
BseGI GGATG 7 cut(s) 295, 489, 574, 653, 675, 735, 928
BseLI CCNNNNNNNGG 5 cut(s) 63, 459, 685, 686, 1010
BseMI GCAATG 2 cut(s) 237, 1044
BseMII CTCAG 2 cut(s) 411, 792
BseNI ACTGG 3 cut(s) 914, 1004, 1087
BseRI GAGGAG 6 cut(s) 19, 41, 142, 145, 818, 986
BseXI GCAGC 3 cut(s) 511, 724, 879
BsgI GTGCAG 3 cut(s) 131, 518, 906
Bsh1236I CGCG 1 cut(s) 458
BshFI GGCC 4 cut(s) 200, 594, 711, 977
BshNI GGYRCC 1 cut(s) 92
BshVI ATCGAT 1 cut(s) 225
BsiHKAI GWGCWC 1 cut(s) 447
BsiHKCI CYCGRG 2 cut(s) 936, 1010
BsiSI CCGG 4 cut(s) 308, 431, 712, 1011
BslFI GGGAC 2 cut(s) 347, 629
BslI CCNNNNNNNGG 5 cut(s) 63, 459, 685, 686, 1010
BsmAI GTCTC 1 cut(s) 180
BsmFI GGGAC 2 cut(s) 347, 629
BsnI GGCC 4 cut(s) 200, 594, 711, 977
BsoBI CYCGRG 2 cut(s) 936, 1010
Bsp119I TTCGAA 1 cut(s) 405
Bsp1286I GDGCHC 1 cut(s) 447
Bsp143I GATC 5 cut(s) 222, 261, 472, 807, 873
BspACI CCGC 6 cut(s) 64, 82, 159, 458, 581, 821
BspANI GGCC 4 cut(s) 200, 594, 711, 977
BspCNI CTCAG 2 cut(s) 412, 793
BspDI ATCGAT 1 cut(s) 225
BspFNI CGCG 1 cut(s) 458
BspLI GGNNCC 5 cut(s) 68, 94, 617, 618, 1023
BspPI GGATC 1 cut(s) 467
BspT104I TTCGAA 1 cut(s) 405
BspT107I GGYRCC 1 cut(s) 92
BsrDI GCAATG 2 cut(s) 237, 1044
BsrI ACTGG 3 cut(s) 914, 1004, 1087
BssECI CCNNGG 6 cut(s) 88, 195, 387, 429, 1010, 1011
BssMI GATC 5 cut(s) 222, 261, 472, 807, 873
BssNI GRCGYC 2 cut(s) 93, 136
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 1 cut(s) 882
Bst4CI ACNGT 1 cut(s) 190
Bst6I CTCTTC 1 cut(s) 80
BstACI GRCGYC 2 cut(s) 93, 136
BstBI TTCGAA 1 cut(s) 405
BstC8I GCNNGC 4 cut(s) 416, 638, 735, 979
BstDEI CTNAG 2 cut(s) 420, 801
BstDSI CCRYGG 2 cut(s) 88, 387
BstF5I GGATG 7 cut(s) 295, 489, 574, 653, 675, 735, 928
BstFNI CGCG 1 cut(s) 458
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 3 cut(s) 95, 363, 414
BstKTI GATC 5 cut(s) 225, 264, 475, 810, 876
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 5 cut(s) 222, 261, 472, 807, 873
BstMWI GCNNNNNNNGC 3 cut(s) 90, 206, 508
BstNI CCWGG 1 cut(s) 882
BstSCI CCNGG 5 cut(s) 307, 429, 880, 1009, 1010
BstUI CGCG 1 cut(s) 458
BstV1I GCAGC 3 cut(s) 511, 724, 879
Bsu15I ATCGAT 1 cut(s) 225
Bsu36I CCTNAGG 2 cut(s) 420, 801
BsuRI GGCC 4 cut(s) 200, 594, 711, 977
BsuTUI ATCGAT 1 cut(s) 225
BtgI CCRYGG 2 cut(s) 88, 387
BtgZI GCGATG 1 cut(s) 439
BtrI CACGTC 1 cut(s) 565
BtsCI GGATG 7 cut(s) 295, 489, 574, 653, 675, 735, 928
BtsIMutI CAGTG 1 cut(s) 201
Cac8I GCNNGC 4 cut(s) 416, 638, 735, 979
CfoI GCGC 3 cut(s) 95, 363, 414
Cfr13I GGNCC 2 cut(s) 616, 1022
Cfr9I CCCGGG 1 cut(s) 1010
ClaI ATCGAT 1 cut(s) 225
CseI GACGC 1 cut(s) 144
CsiI ACCWGGT 1 cut(s) 880
CviAII CATG 2 cut(s) 485, 604
DdeI CTNAG 2 cut(s) 420, 801
DinI GGCGCC 1 cut(s) 94
DpnI GATC 5 cut(s) 224, 263, 474, 809, 875
DpnII GATC 5 cut(s) 222, 261, 472, 807, 873
EaeI YGGCCR 3 cut(s) 198, 592, 709
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
EciI GGCGGA 1 cut(s) 79
Eco130I CCWWGG 1 cut(s) 195
Eco47I GGWCC 2 cut(s) 616, 1022
Eco57I CTGAAG 3 cut(s) 225, 462, 975
Eco81I CCTNAGG 2 cut(s) 420, 801
Eco88I CYCGRG 2 cut(s) 936, 1010
EcoO109I RGGNCCY 1 cut(s) 616
EcoRII CCWGG 1 cut(s) 880
EcoT14I CCWWGG 1 cut(s) 195
EcoT22I ATGCAT 1 cut(s) 653
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 195
FaeI CATG 2 cut(s) 488, 607
FaqI GGGAC 2 cut(s) 347, 629
FatI CATG 2 cut(s) 484, 603
FauI CCCGC 2 cut(s) 451, 588
FauNDI CATATG 1 cut(s) 519
Fnu4HI GCNGC 3 cut(s) 500, 738, 868
FokI GGATG 7 cut(s) 302, 476, 581, 660, 662, 742, 935
Fsp4HI GCNGC 3 cut(s) 500, 738, 868
FspBI CTAG 2 cut(s) 123, 132
FspI TGCGCA 1 cut(s) 362
GlaI GCGC 3 cut(s) 94, 362, 413
GluI GCNGC 3 cut(s) 500, 738, 868
GsuI CTGGAG 1 cut(s) 987
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 4 cut(s) 200, 594, 711, 977
HapII CCGG 4 cut(s) 308, 431, 712, 1011
HgaI GACGC 1 cut(s) 144
HhaI GCGC 3 cut(s) 95, 363, 414
Hin1I GRCGYC 2 cut(s) 93, 136
Hin1II CATG 2 cut(s) 488, 607
Hin6I GCGC 3 cut(s) 93, 361, 412
HinP1I GCGC 3 cut(s) 93, 361, 412
HindIII AAGCTT 1 cut(s) 375
HinfI GANTC 6 cut(s) 344, 383, 425, 796, 906, 931
HpaII CCGG 4 cut(s) 308, 431, 712, 1011
HphI GGTGA 2 cut(s) 569, 871
Hpy188I TCNGA 2 cut(s) 254, 905
Hpy188III TCNNGA 2 cut(s) 786, 800
Hpy99I CGWCG 1 cut(s) 184
HpyAV CCTTC 5 cut(s) 113, 280, 334, 486, 522
HpyCH4III ACNGT 1 cut(s) 190
HpyCH4IV ACGT 1 cut(s) 564
HpyCH4V TGCA 9 cut(s) 148, 269, 293, 499, 636, 651, 664, 751, 887
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 206, 508
HpyF3I CTNAG 2 cut(s) 420, 801
HpySE526I ACGT 1 cut(s) 564
Hsp92I GRCGYC 2 cut(s) 93, 136
Hsp92II CATG 2 cut(s) 488, 607
HspAI GCGC 3 cut(s) 93, 361, 412
KasI GGCGCC 1 cut(s) 92
KflI GGGWCCC 1 cut(s) 616
Kzo9I GATC 5 cut(s) 222, 261, 472, 807, 873
LmnI GCTCC 3 cut(s) 32, 131, 714
Lsp1109I GCAGC 3 cut(s) 511, 724, 879
LweI GCATC 7 cut(s) 218, 280, 498, 638, 684, 720, 751
MabI ACCWGGT 1 cut(s) 880
MaeI CTAG 2 cut(s) 123, 132
MaeII ACGT 1 cut(s) 564
MaeIII GTNAC 1 cut(s) 965
MalI GATC 5 cut(s) 224, 263, 474, 809, 875
MboI GATC 5 cut(s) 222, 261, 472, 807, 873
MboII GAAGA 6 cut(s) 67, 156, 159, 292, 382, 770
MfeI CAATTG 1 cut(s) 294
MhlI GDGCHC 1 cut(s) 447
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 5 cut(s) 294, 397, 705, 891, 996
MluNI TGGCCA 1 cut(s) 200
Mly113I GGCGCC 1 cut(s) 93
MlyI GAGTC 2 cut(s) 338, 392
MmeI TCCRAC 2 cut(s) 883, 934
Mox20I TGGCCA 1 cut(s) 200
Mph1103I ATGCAT 1 cut(s) 653
MscI TGGCCA 1 cut(s) 200
MseI TTAA 2 cut(s) 396, 539
MslI CAYNNNNRTG 4 cut(s) 356, 494, 522, 669
Msp20I TGGCCA 1 cut(s) 200
MspA1I CMGCKG 1 cut(s) 737
MspI CCGG 4 cut(s) 308, 431, 712, 1011
MspR9I CCNGG 5 cut(s) 309, 431, 882, 1011, 1012
MunI CAATTG 1 cut(s) 294
MvaI CCWGG 1 cut(s) 882
MvnI CGCG 1 cut(s) 458
MwoI GCNNNNNNNGC 3 cut(s) 90, 206, 508
NarI GGCGCC 1 cut(s) 93
NciI CCSGG 4 cut(s) 309, 431, 1011, 1012
NdeI CATATG 1 cut(s) 519
NdeII GATC 5 cut(s) 222, 261, 472, 807, 873
NlaIII CATG 2 cut(s) 488, 607
NlaIV GGNNCC 5 cut(s) 68, 94, 617, 618, 1023
NsbI TGCGCA 1 cut(s) 362
NsiI ATGCAT 1 cut(s) 653
NspV TTCGAA 1 cut(s) 405
OliI CACNNNNGTG 1 cut(s) 356
PaeR7I CTCGAG 1 cut(s) 936
PcsI WCGNNNNNNNCGW 1 cut(s) 176
PfeI GAWTC 4 cut(s) 425, 796, 906, 931
PkrI GCNGC 3 cut(s) 501, 739, 869
PleI GAGTC 2 cut(s) 338, 391
PluTI GGCGCC 1 cut(s) 96
PpsI GAGTC 2 cut(s) 338, 391
PpuMI RGGWCCY 1 cut(s) 616
PshBI ATTAAT 1 cut(s) 396
PsiI TTATAA 1 cut(s) 830
Psp5II RGGWCCY 1 cut(s) 616
Psp6I CCWGG 1 cut(s) 880
PspGI CCWGG 1 cut(s) 880
PspN4I GGNNCC 5 cut(s) 68, 94, 617, 618, 1023
PspPI GGNCC 2 cut(s) 616, 1022
PspPPI RGGWCCY 1 cut(s) 616
PspXI VCTCGAGB 1 cut(s) 936
PvuII CAGCTG 1 cut(s) 737
RseI CAYNNNNRTG 4 cut(s) 356, 494, 522, 669
SaqAI TTAA 2 cut(s) 396, 539
SatI GCNGC 3 cut(s) 500, 738, 868
Sau3AI GATC 5 cut(s) 222, 261, 472, 807, 873
Sau96I GGNCC 2 cut(s) 616, 1022
SchI GAGTC 2 cut(s) 338, 392
ScrFI CCNGG 5 cut(s) 309, 431, 882, 1011, 1012
SduI GDGCHC 1 cut(s) 447
SetI ASST 9 cut(s) 128, 379, 504, 567, 700, 739, 872, 883, 943
SexAI ACCWGGT 1 cut(s) 880
SfaNI GCATC 7 cut(s) 218, 280, 498, 638, 684, 720, 751
SfoI GGCGCC 1 cut(s) 94
Sfr274I CTCGAG 1 cut(s) 936
SfuI TTCGAA 1 cut(s) 405
SinI GGWCC 2 cut(s) 616, 1022
SlaI CTCGAG 1 cut(s) 936
SmaI CCCGGG 1 cut(s) 1012
SmiMI CAYNNNNRTG 4 cut(s) 356, 494, 522, 669
SmlI CTYRAG 1 cut(s) 936
SmoI CTYRAG 1 cut(s) 936
Sse9I AATT 5 cut(s) 294, 397, 705, 891, 996
SsiI CCGC 6 cut(s) 64, 82, 159, 458, 581, 821
SspDI GGCGCC 1 cut(s) 92
SspI AATATT 2 cut(s) 589, 721
SspMI CTAG 2 cut(s) 123, 132
StyD4I CCNGG 5 cut(s) 307, 429, 880, 1009, 1010
StyI CCWWGG 1 cut(s) 195
TaaI ACNGT 1 cut(s) 190
TaiI ACGT 1 cut(s) 567
TaqI TCGA 6 cut(s) 8, 225, 278, 405, 872, 937
TasI AATT 5 cut(s) 294, 397, 705, 891, 996
TfiI GAWTC 4 cut(s) 425, 796, 906, 931
Tru1I TTAA 2 cut(s) 396, 539
Tru9I TTAA 2 cut(s) 396, 539
TscAI CASTG 1 cut(s) 208
TseI GCWGC 3 cut(s) 499, 737, 867
TspDTI ATGAA 4 cut(s) 299, 473, 536, 939
TspGWI ACGGA 3 cut(s) 35, 168, 376
TspMI CCCGGG 1 cut(s) 1010
TspRI CASTG 1 cut(s) 208
VpaK11BI GGWCC 2 cut(s) 616, 1022
VspI ATTAAT 1 cut(s) 396
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XhoI CTCGAG 1 cut(s) 936
XmaI CCCGGG 1 cut(s) 1010
XspI CTAG 2 cut(s) 123, 132
Zsp2I ATGCAT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.