Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0013163

Physical Location & Seq

Genomic Coordinates Reverse (-)

30879209 .. 30890990

Gene Structure 11782 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1243500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1017 bp

ATGGCGTCGATGATATTCACAGACTGTTCCTCCTCCACCACCCCTCGCCTCCTCCGCTTCAGACGGAACCCCCTCTTCCACCGCAACCACGGCGCCGTTTTCCTTCGCCCCCTCATAAGCCCTACCTCCCCCAGACGCCTCTCCCTTCACTCCTCCTCGGCTTCTTCTTCGCCGTCTCCGTCAGCCACTGCTTCGTTGGCTACTGAAGCATCAGCAGCAGTGATCGATGGAAAAGGCGTTGCGAAGCAAATCAGAGATGAGATCAATGCCGAAGTGTCAAAGATGAAGGATGCGATTGGCATTGTTCCGGGTTTGGCAGTTATTCTTGTTGGGGACAGGAAGGACTCTGCCACTTATGTCCGCAACAAGAAGAAGGCTTGCGAGTCCGTGGGGATTAAATCCTTCGAAGTGCGCTTGCCTGAGGATTCATCTGAACGAGAAGTGCTAAACTCTGTCGGGAGATTCAATGATGATCCTTCAGTTCATGGCATCCTTGTGCAGCTACCCCTGCCTTCTCATATGAACGAGCAAAACATCTTAAATGCCGTTAGCATTGAGAAAGATGTGGATGGGTTTCACCCGCTAAATATTGGTCGTCTTGCCATGCGTGGTAGGGACCCCTTGTTTGTTCCTTGCACGCCAAAAGGATGCATAGAGTTGTTGCATAGGTATGGCATCCCTATCAAGGGAAAGAGAGCTGTTGTAATTGGCCGGAGCAATATTGTTGGGACGCCAGCTGCTTTATTGCTACAGAGGGAAGATGCAACAATCAGTATAGTCCATTCCCGAACCAAGAATCCTGAGGAGATCACAAGACAAGCGGATATTATAATCTCAGCTGTAGGGCAACCAAATATGGTGAGGGGTAGCTGGATTAAACCCGGTGCAGTAATTATCGATGTCGGAATCAATCCAGTCGAGGGGATAAGGCTTCTCCATCAAGCAATAAGGGGGATTTTACATACATTCTCTCTACTTCCAAATTGGAAGAGAATTTACATCACACCAAAGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004477 GO:0004488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006730 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0019238 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

339

Amino Acids

36.84

Weight (kDa)

9.93

Isoelectric Point (pI)

48.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THF_DHG_CYH	PF00763	75 - 190	6e-44	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C	PF02882	211 - 307	4e-40	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013163)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00600 AT4G00620
fragaria_vesca	FvH4_7g13650 FvH4_7g13650
malus_domestica	MD07G1141500.v1.1 MD08G1243500.v1.1
prunus_persica	Prupe.2G162600_v2.0.a1
pyrus_communis	pycom07g14040 pycom08g21120
rosa_chinensis	RchiOBHm_Chr1g0353731
rosa_laevigata	RLG00000028263
rosa_roxburghii	Rroxscaffold_4G00301710
rosa_rugosa	Rorug01G0237200
rosa_samantha	Rh1AG249400 Rh1BG219500 Rh1CG232100 Rh1DG246500
rosa_wichuraiana	Rw1G021760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	830
AccB1I	GGYRCC	1 cut(s)	92
AciI	CCGC	5 cut(s)	55, 82, 361, 581, 821
AclWI	GGATC	1 cut(s)	467
AcoI	YGGCCR	1 cut(s)	709
AcsI	RAATTY	1 cut(s)	993
AcuI	CTGAAG	3 cut(s)	43, 225, 462
AcyI	GRCGYC	4 cut(s)	5, 93, 136, 731
AfiI	CCNNNNNNNGG	3 cut(s)	685, 686, 920
AgsI	TTSAA	1 cut(s)	466
AloI	GAACNNNNNNTCC	2 cut(s)	781, 813
AluBI	AGCT	5 cut(s)	502, 698, 737, 839, 870
AluI	AGCT	5 cut(s)	502, 698, 737, 839, 870
Alw26I	GTCTC	1 cut(s)	180
AlwI	GGATC	1 cut(s)	467
AlwNI	CAGNNNCTG	1 cut(s)	188
AoxI	GGCC	1 cut(s)	709
ApeKI	GCWGC	3 cut(s)	215, 499, 737
ApoI	RAATTY	1 cut(s)	993
ArsI	GACNNNNNNTTYG	2 cut(s)	608, 640
AspLEI	GCGC	2 cut(s)	95, 414
AspS9I	GGNCC	1 cut(s)	616
AsuC2I	CCSGG	2 cut(s)	309, 882
AsuHPI	GGTGA	2 cut(s)	569, 871
AsuII	TTCGAA	1 cut(s)	405
AvaII	GGWCC	1 cut(s)	616
AxyI	CCTNAGG	2 cut(s)	420, 801
BanI	GGYRCC	1 cut(s)	92
BarI	GAAGNNNNNNTAC	2 cut(s)	980, 1012
BbvI	GCAGC	3 cut(s)	227, 511, 724
BccI	CCATC	3 cut(s)	221, 563, 945
BceAI	ACGGC	4 cut(s)	80, 106, 157, 530
BcnI	CCSGG	2 cut(s)	309, 882
BcoDI	GTCTC	1 cut(s)	180
BfmI	CTRYAG	2 cut(s)	749, 840
BfoI	RGCGCY	1 cut(s)	96
BisI	GCNGC	3 cut(s)	216, 500, 738
BlsI	GCNGC	3 cut(s)	217, 501, 739
Bme1390I	CCNGG	2 cut(s)	309, 882
Bme18I	GGWCC	1 cut(s)	616
BmgT120I	GGNCC	1 cut(s)	616
BmiI	GGNNCC	4 cut(s)	68, 94, 617, 618
BmrFI	CCNGG	2 cut(s)	309, 882
BmsI	GCATC	6 cut(s)	218, 280, 498, 638, 684, 751
Bpu14I	TTCGAA	1 cut(s)	405
BpuMI	CCSGG	2 cut(s)	309, 882
Bsa29I	ATCGAT	2 cut(s)	225, 897
BsaHI	GRCGYC	4 cut(s)	5, 93, 136, 731
BsaJI	CCNNGG	3 cut(s)	88, 156, 387
BsaXI	ACNNNNNCTCC	2 cut(s)	14, 44
Bsc4I	CCNNNNNNNGG	3 cut(s)	685, 686, 920
Bse1I	ACTGG	1 cut(s)	914
Bse21I	CCTNAGG	2 cut(s)	420, 801
BseCI	ATCGAT	2 cut(s)	225, 897
BseDI	CCNNGG	3 cut(s)	88, 156, 387
BseGI	GGATG	5 cut(s)	295, 489, 574, 653, 675
BseLI	CCNNNNNNNGG	3 cut(s)	685, 686, 920
BseMII	CTCAG	3 cut(s)	411, 792, 849
BseNI	ACTGG	1 cut(s)	914
BseRI	GAGGAG	5 cut(s)	22, 41, 142, 145, 818
BseXI	GCAGC	3 cut(s)	227, 511, 724
BsgI	GTGCAG	2 cut(s)	518, 906
BshFI	GGCC	1 cut(s)	711
BshNI	GGYRCC	1 cut(s)	92
BshVI	ATCGAT	2 cut(s)	225, 897
BsiSI	CCGG	3 cut(s)	308, 712, 882
BslFI	GGGAC	3 cut(s)	347, 629, 742
BslI	CCNNNNNNNGG	3 cut(s)	685, 686, 920
BsmAI	GTCTC	1 cut(s)	180
BsmBI	CGTCTC	1 cut(s)	180
BsmFI	GGGAC	3 cut(s)	347, 629, 742
BsnI	GGCC	1 cut(s)	711
Bsp119I	TTCGAA	1 cut(s)	405
Bsp143I	GATC	4 cut(s)	222, 261, 472, 807
BspACI	CCGC	5 cut(s)	55, 82, 361, 581, 821
BspANI	GGCC	1 cut(s)	711
BspCNI	CTCAG	3 cut(s)	412, 793, 848
BspDI	ATCGAT	2 cut(s)	225, 897
BspLI	GGNNCC	4 cut(s)	68, 94, 617, 618
BspPI	GGATC	1 cut(s)	467
BspT104I	TTCGAA	1 cut(s)	405
BspT107I	GGYRCC	1 cut(s)	92
BsrI	ACTGG	1 cut(s)	914
BssECI	CCNNGG	3 cut(s)	88, 156, 387
BssMI	GATC	4 cut(s)	222, 261, 472, 807
BssNI	GRCGYC	4 cut(s)	5, 93, 136, 731
Bst4CI	ACNGT	1 cut(s)	26
Bst6I	CTCTTC	2 cut(s)	80, 983
BstACI	GRCGYC	4 cut(s)	5, 93, 136, 731
BstBI	TTCGAA	1 cut(s)	405
BstC8I	GCNNGC	4 cut(s)	379, 416, 638, 735
BstDEI	CTNAG	3 cut(s)	420, 801, 835
BstDSI	CCRYGG	2 cut(s)	88, 387
BstF5I	GGATG	5 cut(s)	295, 489, 574, 653, 675
BstH2I	RGCGCY	1 cut(s)	96
BstHHI	GCGC	2 cut(s)	95, 414
BstKTI	GATC	4 cut(s)	225, 264, 475, 810
BstMAI	GTCTC	1 cut(s)	180
BstMBI	GATC	4 cut(s)	222, 261, 472, 807
BstMWI	GCNNNNNNNGC	6 cut(s)	54, 90, 197, 206, 215, 508
BstSCI	CCNGG	2 cut(s)	307, 880
BstSFI	CTRYAG	2 cut(s)	749, 840
BstV1I	GCAGC	3 cut(s)	227, 511, 724
Bsu15I	ATCGAT	2 cut(s)	225, 897
Bsu36I	CCTNAGG	2 cut(s)	420, 801
BsuRI	GGCC	1 cut(s)	711
BsuTUI	ATCGAT	2 cut(s)	225, 897
BtgI	CCRYGG	2 cut(s)	88, 387
BtsCI	GGATG	5 cut(s)	295, 489, 574, 653, 675
BtsI	GCAGTG	2 cut(s)	186, 225
BtsIMutI	CAGTG	2 cut(s)	186, 225
Cac8I	GCNNGC	4 cut(s)	379, 416, 638, 735
CaiI	CAGNNNCTG	1 cut(s)	188
CfoI	GCGC	2 cut(s)	95, 414
Cfr13I	GGNCC	1 cut(s)	616
ClaI	ATCGAT	2 cut(s)	225, 897
CseI	GACGC	2 cut(s)	144, 739
CviAII	CATG	2 cut(s)	485, 604
DdeI	CTNAG	3 cut(s)	420, 801, 835
DinI	GGCGCC	1 cut(s)	94
DpnI	GATC	4 cut(s)	224, 263, 474, 809
DpnII	GATC	4 cut(s)	222, 261, 472, 807
EaeI	YGGCCR	1 cut(s)	709
Eam1104I	CTCTTC	2 cut(s)	80, 983
EarI	CTCTTC	2 cut(s)	80, 983
Eco47I	GGWCC	1 cut(s)	616
Eco57I	CTGAAG	3 cut(s)	43, 225, 462
Eco81I	CCTNAGG	2 cut(s)	420, 801
EcoO109I	RGGNCCY	1 cut(s)	616
EcoT22I	ATGCAT	1 cut(s)	653
EgeI	GGCGCC	1 cut(s)	94
EheI	GGCGCC	1 cut(s)	94
Esp3I	CGTCTC	1 cut(s)	180
FaeI	CATG	2 cut(s)	488, 607
FaqI	GGGAC	3 cut(s)	347, 629, 742
FatI	CATG	2 cut(s)	484, 603
FauI	CCCGC	1 cut(s)	588
FauNDI	CATATG	1 cut(s)	519
Fnu4HI	GCNGC	3 cut(s)	216, 500, 738
FokI	GGATG	5 cut(s)	302, 476, 581, 660, 662
Fsp4HI	GCNGC	3 cut(s)	216, 500, 738
GlaI	GCGC	2 cut(s)	94, 413
GluI	GCNGC	3 cut(s)	216, 500, 738
HaeII	RGCGCY	1 cut(s)	96
HaeIII	GGCC	1 cut(s)	711
HapII	CCGG	3 cut(s)	308, 712, 882
HgaI	GACGC	2 cut(s)	144, 739
HhaI	GCGC	2 cut(s)	95, 414
Hin1I	GRCGYC	4 cut(s)	5, 93, 136, 731
Hin1II	CATG	2 cut(s)	488, 607
Hin6I	GCGC	2 cut(s)	93, 412
HinP1I	GCGC	2 cut(s)	93, 412
HinfI	GANTC	6 cut(s)	344, 383, 425, 462, 796, 906
HpaII	CCGG	3 cut(s)	308, 712, 882
HphI	GGTGA	2 cut(s)	569, 871
Hpy188I	TCNGA	4 cut(s)	62, 254, 433, 905
Hpy188III	TCNNGA	3 cut(s)	457, 786, 800
Hpy99I	CGWCG	1 cut(s)	10
HpyAV	CCTTC	8 cut(s)	113, 155, 280, 334, 367, 412, 486, 522
HpyCH4III	ACNGT	1 cut(s)	26
HpyCH4V	TGCA	6 cut(s)	499, 636, 651, 664, 764, 887
HpyF10VI	GCNNNNNNNGC	6 cut(s)	54, 90, 197, 206, 215, 508
HpyF3I	CTNAG	3 cut(s)	420, 801, 835
Hsp92I	GRCGYC	4 cut(s)	5, 93, 136, 731
Hsp92II	CATG	2 cut(s)	488, 607
HspAI	GCGC	2 cut(s)	93, 412
KasI	GGCGCC	1 cut(s)	92
KflI	GGGWCCC	1 cut(s)	616
Kzo9I	GATC	4 cut(s)	222, 261, 472, 807
LmnI	GCTCC	1 cut(s)	714
Lsp1109I	GCAGC	3 cut(s)	227, 511, 724
LweI	GCATC	6 cut(s)	218, 280, 498, 638, 684, 751
MalI	GATC	4 cut(s)	224, 263, 474, 809
MboI	GATC	4 cut(s)	222, 261, 472, 807
MboII	GAAGA	6 cut(s)	67, 156, 159, 382, 770, 1000
MluCI	AATT	4 cut(s)	705, 891, 982, 993
Mly113I	GGCGCC	1 cut(s)	93
MlyI	GAGTC	2 cut(s)	338, 392
MmeI	TCCRAC	1 cut(s)	883
Mph1103I	ATGCAT	1 cut(s)	653
MseI	TTAA	3 cut(s)	396, 539, 876
MslI	CAYNNNNRTG	2 cut(s)	494, 669
MspA1I	CMGCKG	2 cut(s)	737, 839
MspI	CCGG	3 cut(s)	308, 712, 882
MspR9I	CCNGG	2 cut(s)	309, 882
MwoI	GCNNNNNNNGC	6 cut(s)	54, 90, 197, 206, 215, 508
NarI	GGCGCC	1 cut(s)	93
NciI	CCSGG	2 cut(s)	309, 882
NdeI	CATATG	1 cut(s)	519
NdeII	GATC	4 cut(s)	222, 261, 472, 807
NlaIII	CATG	2 cut(s)	488, 607
NlaIV	GGNNCC	4 cut(s)	68, 94, 617, 618
NmeAIII	GCCGAG	1 cut(s)	137
NsiI	ATGCAT	1 cut(s)	653
NspV	TTCGAA	1 cut(s)	405
PcsI	WCGNNNNNNNCGW	1 cut(s)	176
PfeI	GAWTC	4 cut(s)	425, 462, 796, 906
PkrI	GCNGC	3 cut(s)	217, 501, 739
PleI	GAGTC	2 cut(s)	338, 391
PluTI	GGCGCC	1 cut(s)	96
PpsI	GAGTC	2 cut(s)	338, 391
PpuMI	RGGWCCY	1 cut(s)	616
PsiI	TTATAA	1 cut(s)	830
Psp5II	RGGWCCY	1 cut(s)	616
PspN4I	GGNNCC	4 cut(s)	68, 94, 617, 618
PspPI	GGNCC	1 cut(s)	616
PspPPI	RGGWCCY	1 cut(s)	616
PstNI	CAGNNNCTG	1 cut(s)	188
PvuII	CAGCTG	2 cut(s)	737, 839
RseI	CAYNNNNRTG	2 cut(s)	494, 669
SaqAI	TTAA	3 cut(s)	396, 539, 876
SatI	GCNGC	3 cut(s)	216, 500, 738
Sau3AI	GATC	4 cut(s)	222, 261, 472, 807
Sau96I	GGNCC	1 cut(s)	616
SchI	GAGTC	2 cut(s)	338, 392
ScrFI	CCNGG	2 cut(s)	309, 882
SetI	ASST	7 cut(s)	128, 504, 671, 700, 739, 841, 872
SfaNI	GCATC	6 cut(s)	218, 280, 498, 638, 684, 751
SfcI	CTRYAG	2 cut(s)	749, 840
SfoI	GGCGCC	1 cut(s)	94
SfuI	TTCGAA	1 cut(s)	405
SinI	GGWCC	1 cut(s)	616
SmiMI	CAYNNNNRTG	2 cut(s)	494, 669
Sse9I	AATT	4 cut(s)	705, 891, 982, 993
SsiI	CCGC	5 cut(s)	55, 82, 361, 581, 821
SspDI	GGCGCC	1 cut(s)	92
SspI	AATATT	2 cut(s)	589, 721
StyD4I	CCNGG	2 cut(s)	307, 880
TaaI	ACNGT	1 cut(s)	26
TaqI	TCGA	5 cut(s)	8, 225, 405, 897, 918
TasI	AATT	4 cut(s)	705, 891, 982, 993
TfiI	GAWTC	4 cut(s)	425, 462, 796, 906
Tru1I	TTAA	3 cut(s)	396, 539, 876
Tru9I	TTAA	3 cut(s)	396, 539, 876
TscAI	CASTG	2 cut(s)	193, 225
TseI	GCWGC	3 cut(s)	215, 499, 737
TspDTI	ATGAA	4 cut(s)	299, 417, 473, 536
TspGWI	ACGGA	3 cut(s)	79, 168, 376
TspRI	CASTG	2 cut(s)	193, 225
VpaK11BI	GGWCC	1 cut(s)	616
XapI	RAATTY	1 cut(s)	993
XcmI	CCANNNNNNNNNTGG	1 cut(s)	193
Zsp2I	ATGCAT	1 cut(s)	653

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.