pycom08g21120

Bifunctional protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013163
Physical Location & Seq
Reverse (-)
20692395 .. 20694574
2180 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g21120.2

Sequence Viewer

Length: 1092 bp
ATGTCGTCGATGATATTCACGGACTGTTCCTCCTCCACCACCCCTCGCCTCCTCCGATTCAGACGGAACCCCCTCTTCCACCGCAACCACGGCGCCGTTTTCCTGCGCCCCCTCATAAGCCCTAGCTCCCCCAGACGCCTCTCCCTTTACTCCTCGTCTGCTTCTCCTTCGCCGTCTCCATCAGCAACAGCTTCGTTGGCTACTGAAGCATCAGCGGCAGTGATCGATGGAAAAGGCGTTGCGAAACAAATCAGAGATGAGATCAATGCCGAAGTGTCAAAGATGAAGGATGCGATTGGCATTGTTCCGGGTTTGGCAGTTATTCTTGTTGGGGACAGGAAGGACTCTGCCACCTATGTCCGCAACAAGAAGAAAGCTTGTGAGTCCGTGGGGATTAATTCCTTCGAAGTGCGCTTGCCTGAGGATTCAACTGAACGAGAAGTGCTAAACTCTGTCGCCAGATTCAATGATGATCCTGCAGTTCATGGCATCCTTGTGCAGCTACCCCTGCCTTCTCATATGAACGAGCAAAACATCTTAAATTCCGTTAGCATTGAGAAAGATGTGGATGGGTTTCACCCGCTAAATATTGGCCGTCTTGCCATGCGTGGTAGGGACCCCTTGTTTGTTCCTTGCACGCCAAAAGGATGCATAGAGTTGTTGCATAGGTATGGCATCCCTATCAAGGGAAAGAGAGCTGTTGTAATTGGCCGGAGTAATATTGTTGGGATGCCAGCTGCTTTATTGCTGCAGAGGGAAGATGCGACAATCAGCATAGTCCATTCCCGAACCAAGAATCCTGAGGATATCACAAGACAAGCGGATATTATAATCTCAGCTGTAGGGCAACCAAATATGGTGAGGGGTAGCTGGATCAAACCTGGTGCAGTAATTATTGATGTCGGAATCAATCCAGTCGAGGATAAAAAGAGCCCTCGAGGTTACCGGCTGGTCGGAGATGTGTGTTATGAGGAGGCCAGCCAGATTGCTTCGGCAATAACTCCAGTTCCTGGGGGAGTGGGTCCAATGACAATAGCAATGCTTCTTTCGAACACTCTTGCATCATCAAAACGAATACACAACTTCCAGTGA

Protein Analysis

364

Amino Acids

39.23

Weight (kDa)

9.49

Isoelectric Point (pI)

52.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013163)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00600 AT4G00620
fragaria_vesca FvH4_7g13650 FvH4_7g13650
malus_domestica MD07G1141500.v1.1 MD08G1243500.v1.1
prunus_persica Prupe.2G162600_v2.0.a1
pyrus_communis pycom07g14040 pycom08g21120
rosa_chinensis RchiOBHm_Chr1g0353731
rosa_laevigata RLG00000028263
rosa_roxburghii Rroxscaffold_4G00301710
rosa_rugosa Rorug01G0237200
rosa_samantha Rh1AG249400 Rh1BG219500 Rh1CG232100 Rh1DG246500
rosa_wichuraiana Rw1G021760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AbsI CCTCGAGG 1 cut(s) 936
AccB1I GGYRCC 1 cut(s) 92
AccB7I CCANNNNNTGG 1 cut(s) 1010
AciI CCGC 5 cut(s) 82, 215, 361, 581, 821
AclWI GGATC 2 cut(s) 467, 881
AcoI YGGCCR 2 cut(s) 592, 709
AcsI RAATTY 1 cut(s) 541
AcuI CTGAAG 1 cut(s) 225
AcyI GRCGYC 2 cut(s) 93, 136
AfiI CCNNNNNNNGG 3 cut(s) 685, 686, 1010
AgsI TTSAA 2 cut(s) 429, 466
AjnI CCWGG 2 cut(s) 880, 1009
AloI GAACNNNNNNTCC 2 cut(s) 781, 813
AluBI AGCT 8 cut(s) 126, 191, 377, 502, 698, 737, 839, 870
AluI AGCT 8 cut(s) 126, 191, 377, 502, 698, 737, 839, 870
Alw26I GTCTC 1 cut(s) 180
AlwI GGATC 2 cut(s) 467, 881
AlwNI CAGNNNCTG 1 cut(s) 1010
Ama87I CYCGRG 1 cut(s) 936
AoxI GGCC 3 cut(s) 592, 709, 975
ApeKI GCWGC 3 cut(s) 499, 737, 748
ApoI RAATTY 1 cut(s) 541
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
AseI ATTAAT 1 cut(s) 396
AspLEI GCGC 3 cut(s) 95, 108, 414
AspS9I GGNCC 2 cut(s) 616, 1022
AsuC2I CCSGG 1 cut(s) 309
AsuHPI GGTGA 2 cut(s) 569, 871
AsuII TTCGAA 2 cut(s) 405, 1049
AvaI CYCGRG 1 cut(s) 936
AvaII GGWCC 2 cut(s) 616, 1022
AxyI CCTNAGG 2 cut(s) 420, 801
BanI GGYRCC 1 cut(s) 92
BanII GRGCYC 1 cut(s) 935
BbvI GCAGC 3 cut(s) 511, 724, 735
BccI CCATC 3 cut(s) 187, 221, 563
BceAI ACGGC 4 cut(s) 80, 106, 157, 579
BcgI CGANNNNNNTGC 2 cut(s) 174, 208
BciT130I CCWGG 2 cut(s) 882, 1011
BcnI CCSGG 1 cut(s) 309
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 1 cut(s) 123
BfmI CTRYAG 3 cut(s) 477, 749, 840
BfoI RGCGCY 1 cut(s) 96
BisI GCNGC 4 cut(s) 216, 500, 738, 749
BlsI GCNGC 4 cut(s) 217, 501, 739, 750
Bme1390I CCNGG 3 cut(s) 309, 882, 1011
Bme18I GGWCC 2 cut(s) 616, 1022
BmeT110I CYCGRG 1 cut(s) 936
BmgT120I GGNCC 2 cut(s) 616, 1022
BmiI GGNNCC 5 cut(s) 68, 94, 617, 618, 1023
BmrFI CCNGG 3 cut(s) 309, 882, 1011
BmsI GCATC 8 cut(s) 218, 280, 498, 638, 684, 720, 751, 1070
BpmI CTGGAG 1 cut(s) 987
Bpu14I TTCGAA 2 cut(s) 405, 1049
BpuMI CCSGG 1 cut(s) 309
Bsa29I ATCGAT 1 cut(s) 225
BsaHI GRCGYC 2 cut(s) 93, 136
BsaJI CCNNGG 3 cut(s) 88, 387, 1010
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bsc4I CCNNNNNNNGG 3 cut(s) 685, 686, 1010
Bse118I RCCGGY 1 cut(s) 945
Bse1I ACTGG 3 cut(s) 914, 1004, 1087
Bse21I CCTNAGG 2 cut(s) 420, 801
Bse3DI GCAATG 1 cut(s) 1044
BseBI CCWGG 2 cut(s) 882, 1011
BseCI ATCGAT 1 cut(s) 225
BseDI CCNNGG 3 cut(s) 88, 387, 1010
BseGI GGATG 6 cut(s) 295, 489, 574, 653, 675, 735
BseLI CCNNNNNNNGG 3 cut(s) 685, 686, 1010
BseMI GCAATG 1 cut(s) 1044
BseMII CTCAG 3 cut(s) 411, 792, 849
BseNI ACTGG 3 cut(s) 914, 1004, 1087
BseRI GAGGAG 4 cut(s) 22, 41, 142, 986
BseXI GCAGC 3 cut(s) 511, 724, 735
BsgI GTGCAG 2 cut(s) 518, 906
BshFI GGCC 3 cut(s) 594, 711, 977
BshNI GGYRCC 1 cut(s) 92
BshVI ATCGAT 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 936
BsiSI CCGG 3 cut(s) 308, 712, 946
BslFI GGGAC 2 cut(s) 347, 629
BslI CCNNNNNNNGG 3 cut(s) 685, 686, 1010
BsmAI GTCTC 1 cut(s) 180
BsmBI CGTCTC 1 cut(s) 180
BsmFI GGGAC 2 cut(s) 347, 629
BsnI GGCC 3 cut(s) 594, 711, 977
BsoBI CYCGRG 1 cut(s) 936
Bsp119I TTCGAA 2 cut(s) 405, 1049
Bsp1286I GDGCHC 1 cut(s) 935
Bsp143I GATC 4 cut(s) 222, 261, 472, 873
BspACI CCGC 5 cut(s) 82, 215, 361, 581, 821
BspANI GGCC 3 cut(s) 594, 711, 977
BspCNI CTCAG 3 cut(s) 412, 793, 848
BspDI ATCGAT 1 cut(s) 225
BspLI GGNNCC 5 cut(s) 68, 94, 617, 618, 1023
BspMAI CTGCAG 2 cut(s) 481, 753
BspPI GGATC 2 cut(s) 467, 881
BspT104I TTCGAA 2 cut(s) 405, 1049
BspT107I GGYRCC 1 cut(s) 92
BsrDI GCAATG 1 cut(s) 1044
BsrFI RCCGGY 1 cut(s) 945
BsrI ACTGG 3 cut(s) 914, 1004, 1087
BssAI RCCGGY 1 cut(s) 945
BssECI CCNNGG 3 cut(s) 88, 387, 1010
BssMI GATC 4 cut(s) 222, 261, 472, 873
BssNI GRCGYC 2 cut(s) 93, 136
Bst2UI CCWGG 2 cut(s) 882, 1011
Bst4CI ACNGT 1 cut(s) 26
Bst6I CTCTTC 1 cut(s) 80
BstACI GRCGYC 2 cut(s) 93, 136
BstBI TTCGAA 2 cut(s) 405, 1049
BstC8I GCNNGC 4 cut(s) 416, 638, 735, 979
BstDEI CTNAG 3 cut(s) 420, 801, 835
BstDSI CCRYGG 2 cut(s) 88, 387
BstEII GGTNACC 1 cut(s) 941
BstF5I GGATG 6 cut(s) 295, 489, 574, 653, 675, 735
BstH2I RGCGCY 1 cut(s) 96
BstHHI GCGC 3 cut(s) 95, 108, 414
BstKTI GATC 4 cut(s) 225, 264, 475, 876
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 4 cut(s) 222, 261, 472, 873
BstMWI GCNNNNNNNGC 5 cut(s) 90, 197, 206, 215, 508
BstNI CCWGG 2 cut(s) 882, 1011
BstPI GGTNACC 1 cut(s) 941
BstSCI CCNGG 3 cut(s) 307, 880, 1009
BstSFI CTRYAG 3 cut(s) 477, 749, 840
BstV1I GCAGC 3 cut(s) 511, 724, 735
Bsu15I ATCGAT 1 cut(s) 225
Bsu36I CCTNAGG 2 cut(s) 420, 801
BsuRI GGCC 3 cut(s) 594, 711, 977
BsuTUI ATCGAT 1 cut(s) 225
BtgI CCRYGG 2 cut(s) 88, 387
BtsCI GGATG 6 cut(s) 295, 489, 574, 653, 675, 735
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 225
Cac8I GCNNGC 4 cut(s) 416, 638, 735, 979
CaiI CAGNNNCTG 1 cut(s) 1010
CfoI GCGC 3 cut(s) 95, 108, 414
Cfr10I RCCGGY 1 cut(s) 945
Cfr13I GGNCC 2 cut(s) 616, 1022
ClaI ATCGAT 1 cut(s) 225
CseI GACGC 1 cut(s) 144
CsiI ACCWGGT 1 cut(s) 880
CviAII CATG 2 cut(s) 485, 604
DdeI CTNAG 3 cut(s) 420, 801, 835
DinI GGCGCC 1 cut(s) 94
DpnI GATC 4 cut(s) 224, 263, 474, 875
DpnII GATC 4 cut(s) 222, 261, 472, 873
EaeI YGGCCR 2 cut(s) 592, 709
Eam1104I CTCTTC 1 cut(s) 80
EarI CTCTTC 1 cut(s) 80
Eco24I GRGCYC 1 cut(s) 935
Eco32I GATATC 1 cut(s) 808
Eco47I GGWCC 2 cut(s) 616, 1022
Eco57I CTGAAG 1 cut(s) 225
Eco81I CCTNAGG 2 cut(s) 420, 801
Eco88I CYCGRG 1 cut(s) 936
Eco91I GGTNACC 1 cut(s) 941
EcoO109I RGGNCCY 1 cut(s) 616
EcoO65I GGTNACC 1 cut(s) 941
EcoRII CCWGG 2 cut(s) 880, 1009
EcoRV GATATC 1 cut(s) 808
EcoT22I ATGCAT 1 cut(s) 653
EcoT38I GRGCYC 1 cut(s) 935
EgeI GGCGCC 1 cut(s) 94
EheI GGCGCC 1 cut(s) 94
Esp3I CGTCTC 1 cut(s) 180
FaeI CATG 2 cut(s) 488, 607
FaqI GGGAC 2 cut(s) 347, 629
FatI CATG 2 cut(s) 484, 603
FauI CCCGC 1 cut(s) 588
FauNDI CATATG 1 cut(s) 519
Fnu4HI GCNGC 4 cut(s) 216, 500, 738, 749
FokI GGATG 6 cut(s) 302, 476, 581, 660, 662, 742
FriOI GRGCYC 1 cut(s) 935
Fsp4HI GCNGC 4 cut(s) 216, 500, 738, 749
FspBI CTAG 1 cut(s) 123
GlaI GCGC 3 cut(s) 94, 107, 413
GluI GCNGC 4 cut(s) 216, 500, 738, 749
GsuI CTGGAG 1 cut(s) 987
HaeII RGCGCY 1 cut(s) 96
HaeIII GGCC 3 cut(s) 594, 711, 977
HapII CCGG 3 cut(s) 308, 712, 946
HgaI GACGC 1 cut(s) 144
HhaI GCGC 3 cut(s) 95, 108, 414
Hin1I GRCGYC 2 cut(s) 93, 136
Hin1II CATG 2 cut(s) 488, 607
Hin6I GCGC 3 cut(s) 93, 106, 412
HinP1I GCGC 3 cut(s) 93, 106, 412
HindIII AAGCTT 1 cut(s) 375
HinfI GANTC 7 cut(s) 57, 344, 383, 425, 462, 796, 906
HpaII CCGG 3 cut(s) 308, 712, 946
HphI GGTGA 2 cut(s) 569, 871
Hpy188I TCNGA 5 cut(s) 56, 62, 254, 905, 956
Hpy188III TCNNGA 2 cut(s) 786, 800
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 5 cut(s) 177, 280, 334, 412, 522
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4V TGCA 8 cut(s) 479, 499, 636, 651, 664, 751, 887, 1061
HpyF10VI GCNNNNNNNGC 5 cut(s) 90, 197, 206, 215, 508
HpyF3I CTNAG 3 cut(s) 420, 801, 835
Hsp92I GRCGYC 2 cut(s) 93, 136
Hsp92II CATG 2 cut(s) 488, 607
HspAI GCGC 3 cut(s) 93, 106, 412
KasI GGCGCC 1 cut(s) 92
KflI GGGWCCC 1 cut(s) 616
Kzo9I GATC 4 cut(s) 222, 261, 472, 873
LmnI GCTCC 1 cut(s) 131
Lsp1109I GCAGC 3 cut(s) 511, 724, 735
LweI GCATC 8 cut(s) 218, 280, 498, 638, 684, 720, 751, 1070
MabI ACCWGGT 1 cut(s) 880
MaeI CTAG 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 941
MalI GATC 4 cut(s) 224, 263, 474, 875
MboI GATC 4 cut(s) 222, 261, 472, 873
MboII GAAGA 3 cut(s) 67, 382, 770
MhlI GDGCHC 1 cut(s) 935
MluCI AATT 4 cut(s) 397, 541, 705, 891
Mly113I GGCGCC 1 cut(s) 93
MlyI GAGTC 2 cut(s) 338, 392
MmeI TCCRAC 2 cut(s) 883, 934
Mph1103I ATGCAT 1 cut(s) 653
MseI TTAA 2 cut(s) 396, 539
MslI CAYNNNNRTG 2 cut(s) 494, 669
MspA1I CMGCKG 3 cut(s) 215, 737, 839
MspI CCGG 3 cut(s) 308, 712, 946
MspR9I CCNGG 3 cut(s) 309, 882, 1011
MvaI CCWGG 2 cut(s) 882, 1011
MwoI GCNNNNNNNGC 5 cut(s) 90, 197, 206, 215, 508
NarI GGCGCC 1 cut(s) 93
NciI CCSGG 1 cut(s) 309
NdeI CATATG 1 cut(s) 519
NdeII GATC 4 cut(s) 222, 261, 472, 873
NlaIII CATG 2 cut(s) 488, 607
NlaIV GGNNCC 5 cut(s) 68, 94, 617, 618, 1023
NsiI ATGCAT 1 cut(s) 653
NspV TTCGAA 2 cut(s) 405, 1049
PaeR7I CTCGAG 1 cut(s) 936
PcsI WCGNNNNNNNCGW 1 cut(s) 52
PfeI GAWTC 5 cut(s) 57, 425, 462, 796, 906
PflMI CCANNNNNTGG 1 cut(s) 1010
PkrI GCNGC 4 cut(s) 217, 501, 739, 750
PleI GAGTC 2 cut(s) 338, 391
PluTI GGCGCC 1 cut(s) 96
PpsI GAGTC 2 cut(s) 338, 391
PpuMI RGGWCCY 1 cut(s) 616
PshBI ATTAAT 1 cut(s) 396
PsiI TTATAA 1 cut(s) 830
Psp5II RGGWCCY 1 cut(s) 616
Psp6I CCWGG 2 cut(s) 880, 1009
PspEI GGTNACC 1 cut(s) 941
PspGI CCWGG 2 cut(s) 880, 1009
PspN4I GGNNCC 5 cut(s) 68, 94, 617, 618, 1023
PspPI GGNCC 2 cut(s) 616, 1022
PspPPI RGGWCCY 1 cut(s) 616
PspXI VCTCGAGB 1 cut(s) 936
PstI CTGCAG 2 cut(s) 481, 753
PstNI CAGNNNCTG 1 cut(s) 1010
PvuII CAGCTG 2 cut(s) 737, 839
RseI CAYNNNNRTG 2 cut(s) 494, 669
SaqAI TTAA 2 cut(s) 396, 539
SatI GCNGC 4 cut(s) 216, 500, 738, 749
Sau3AI GATC 4 cut(s) 222, 261, 472, 873
Sau96I GGNCC 2 cut(s) 616, 1022
SchI GAGTC 2 cut(s) 338, 392
ScrFI CCNGG 3 cut(s) 309, 882, 1011
SduI GDGCHC 1 cut(s) 935
SexAI ACCWGGT 1 cut(s) 880
SfaNI GCATC 8 cut(s) 218, 280, 498, 638, 684, 720, 751, 1070
SfcI CTRYAG 3 cut(s) 477, 749, 840
SfoI GGCGCC 1 cut(s) 94
Sfr274I CTCGAG 1 cut(s) 936
SfuI TTCGAA 2 cut(s) 405, 1049
SinI GGWCC 2 cut(s) 616, 1022
SlaI CTCGAG 1 cut(s) 936
SmiMI CAYNNNNRTG 2 cut(s) 494, 669
SmlI CTYRAG 1 cut(s) 936
SmoI CTYRAG 1 cut(s) 936
Sse9I AATT 4 cut(s) 397, 541, 705, 891
SsiI CCGC 5 cut(s) 82, 215, 361, 581, 821
SspDI GGCGCC 1 cut(s) 92
SspI AATATT 2 cut(s) 589, 721
SspMI CTAG 1 cut(s) 123
StyD4I CCNGG 3 cut(s) 307, 880, 1009
TaaI ACNGT 1 cut(s) 26
TaqI TCGA 6 cut(s) 8, 225, 405, 918, 937, 1049
TasI AATT 4 cut(s) 397, 541, 705, 891
TauI GCSGC 1 cut(s) 218
TfiI GAWTC 5 cut(s) 57, 425, 462, 796, 906
Tru1I TTAA 2 cut(s) 396, 539
Tru9I TTAA 2 cut(s) 396, 539
TscAI CASTG 1 cut(s) 225
TseI GCWGC 3 cut(s) 499, 737, 748
TspDTI ATGAA 3 cut(s) 299, 473, 536
TspGWI ACGGA 4 cut(s) 35, 79, 376, 535
TspRI CASTG 1 cut(s) 225
Van91I CCANNNNNTGG 1 cut(s) 1010
VpaK11BI GGWCC 2 cut(s) 616, 1022
VspI ATTAAT 1 cut(s) 396
XapI RAATTY 1 cut(s) 541
XhoI CTCGAG 1 cut(s) 936
XspI CTAG 1 cut(s) 123
Zsp2I ATGCAT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.