Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0013163

Physical Location & Seq

Genomic Coordinates Forward (+)

22054493 .. 22057747

Gene Structure 3255 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00301710.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1140 bp

ATGGCGGCAGCGTCGATGGTATTCACGGACTGCTGCTCCTCCTCCACCACCGCCGCCGCTCTCCTCCTCCCCTTCACACGTACCACCCCTCTCCTCCTCCGCACCACCCAATACGCCGTCGTTTTCCCCCGCCGCCTCATCCACCCTCCCAGAACCCTCTCAATCCACTCCTCTTCTTCTTCCCCCTCCCAATTGTCCACCGCTTCAATTGTTTCCCAAAATGAGAAGCACGAAAAATCGGATACGAAACTGGAAATAGTGAATAAAAGGCTTGATGGGAAGAAACTGGCAGAGAAAATTAGAGGGGAGATAGCTGAGGAAGTGTCAAGGATGAAGGATGCAATTGGGGTTGTTCCGGGTTTGGCTGTTATTTTGGTTGGAGATAGGGAAGAATCTGCTACTTATGTTAGCAAGAAGAAGGAGGCTTGTGAGGTTGTGGGGATTACTTCTTTTGAAGTGCATTTGCCGGAGGATGCCACGGAACAAGAAGTGCTCGACTCCATCTCCGGCTTCAATGAGGATCCCTCAGTTCATGGCATCCTTGTTCAGCTACCTCTGCCTCGGCATATGGATGAACAAAACATTTTAAATGCTGTTAGCATTGAGAAAGATGTGGATGGATTTCACCCGCTAAATATTGGCTGTCTTGCCATGCGAGGTAGGGAACCCTTCTTTGTTCCCTGTACTCCCAAAGGCTGCATAGAGCTGTTGCATAGATATGACATCCCTATCAAAGGAAAGAGAGCTGTTGTAATTGGCCGGAGCAATATTGTTGGGATGCCAGTTGCTTTGTTGCTGCAGAGGGAAGATGCTACAGTCAGCATAGTCCATTCCCGAACCAAGAATCCCGAAGAGTTAACAAAACAAGCAGATATTGTAATCGCAGCTGTAGGGCAAGCAAATATGGTGAGGGGTAGCTGGATTAAACCTGGTGCAGTAGTTATTGATGTCGGAATCAATCCAGTTGAGGATAAAACAAAGCGTGCGGGCTTTCGGCTAGTTGGAGATGTCTGTTATAAGGAGGCCAAAGAAATTGCTTCAGCAATTACTCCAGTACCAGGAGGAGTTGGTCCAATGACTATAGCAATGCTTCTCACTAATACACTCATATCAGCAAAAAGGATACACAACTTCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004477 GO:0004488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006730 GO:0008150 GO:0008152 GO:0009507 GO:0009536 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0016787 GO:0016810 GO:0016814 GO:0019238 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

THF_DHG_CYH THF_DHG_CYH_C

Protein Analysis

379

Amino Acids

41.14

Weight (kDa)

7.65

Isoelectric Point (pI)

47.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
THF_DHG_CYH	PF00763	91 - 206	7e-43	Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
THF_DHG_CYH_C	PF02882	227 - 374	3.6e-62	Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013163)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00600 AT4G00620
fragaria_vesca	FvH4_7g13650 FvH4_7g13650
malus_domestica	MD07G1141500.v1.1 MD08G1243500.v1.1
prunus_persica	Prupe.2G162600_v2.0.a1
pyrus_communis	pycom07g14040 pycom08g21120
rosa_chinensis	RchiOBHm_Chr1g0353731
rosa_laevigata	RLG00000028263
rosa_roxburghii	Rroxscaffold_4G00301710
rosa_rugosa	Rorug01G0237200
rosa_samantha	Rh1AG249400 Rh1BG219500 Rh1CG232100 Rh1DG246500
rosa_wichuraiana	Rw1G021760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1017
AccBSI	CCGCTC	1 cut(s)	59
AclWI	GGATC	2 cut(s)	515, 528
AcoI	YGGCCR	1 cut(s)	757
AcuI	CTGAAG	1 cut(s)	1023
AfaI	GTAC	3 cut(s)	82, 685, 1056
AfiI	CCNNNNNNNGG	2 cut(s)	734, 1058
AflIII	ACRYGT	1 cut(s)	77
AgsI	TTSAA	3 cut(s)	207, 455, 514
AjnI	CCWGG	2 cut(s)	928, 1057
AloI	GAACNNNNNNTCC	2 cut(s)	829, 861
AluBI	AGCT	6 cut(s)	314, 550, 706, 746, 887, 918
AluI	AGCT	6 cut(s)	314, 550, 706, 746, 887, 918
Alw21I	GWGCWC	1 cut(s)	495
AlwI	GGATC	2 cut(s)	515, 528
AoxI	GGCC	2 cut(s)	757, 1023
ApeKI	GCWGC	5 cut(s)	8, 33, 696, 796, 884
AspS9I	GGNCC	1 cut(s)	1070
AsuC2I	CCSGG	1 cut(s)	357
AsuHPI	GGTGA	2 cut(s)	617, 919
AvaII	GGWCC	1 cut(s)	1070
BaeI	ACNNNNGTAYC	2 cut(s)	1038, 1071
BamHI	GGATCC	1 cut(s)	520
Bbv12I	GWGCWC	1 cut(s)	495
BbvCI	CCTCAGC	1 cut(s)	315
BbvI	GCAGC	5 cut(s)	20, 20, 683, 783, 896
BccI	CCATC	4 cut(s)	10, 269, 509, 611
BceAI	ACGGC	1 cut(s)	101
BciT130I	CCWGG	2 cut(s)	930, 1059
BciVI	GTATCC	2 cut(s)	235, 1116
BcnI	CCSGG	1 cut(s)	357
BfaI	CTAG	1 cut(s)	998
BfmI	CTRYAG	4 cut(s)	797, 813, 888, 1080
BfuI	GTATCC	2 cut(s)	235, 1116
BisI	GCNGC	9 cut(s)	6, 9, 34, 54, 57, 133, 697, 797, 885
BlsI	GCNGC	9 cut(s)	7, 10, 35, 55, 58, 134, 698, 798, 886
Bme1390I	CCNGG	3 cut(s)	357, 930, 1059
Bme18I	GGWCC	1 cut(s)	1070
BmgT120I	GGNCC	1 cut(s)	1070
BmiI	GGNNCC	2 cut(s)	522, 666
BmrFI	CCNGG	3 cut(s)	357, 930, 1059
BmsI	GCATC	5 cut(s)	328, 463, 546, 768, 799
BplI	GAGNNNNNCTC	2 cut(s)	509, 541
BpmI	CTGGAG	1 cut(s)	1035
Bpu10I	CCTNAGC	1 cut(s)	315
BpuMI	CCSGG	1 cut(s)	357
BsaAI	YACGTR	1 cut(s)	80
BsaJI	CCNNGG	2 cut(s)	477, 560
BsaXI	ACNNNNNCTCC	4 cut(s)	20, 50, 488, 518
Bsc4I	CCNNNNNNNGG	2 cut(s)	734, 1058
Bse1I	ACTGG	6 cut(s)	255, 291, 782, 962, 1052, 1135
Bse3DI	GCAATG	1 cut(s)	1092
BseBI	CCWGG	2 cut(s)	930, 1059
BseDI	CCNNGG	2 cut(s)	477, 560
BseGI	GGATG	9 cut(s)	138, 336, 343, 478, 537, 577, 622, 723, 783
BseLI	CCNNNNNNNGG	2 cut(s)	734, 1058
BseMI	GCAATG	1 cut(s)	1092
BseMII	CTCAG	2 cut(s)	306, 540
BseNI	ACTGG	6 cut(s)	255, 291, 782, 962, 1052, 1135
BseRI	GAGGAG	8 cut(s)	28, 31, 53, 56, 83, 86, 160, 1077
BseXI	GCAGC	5 cut(s)	20, 20, 683, 783, 896
BsgI	GTGCAG	1 cut(s)	954
BshFI	GGCC	2 cut(s)	759, 1025
BsiHKAI	GWGCWC	1 cut(s)	495
BsiSI	CCGG	4 cut(s)	356, 467, 507, 760
BslI	CCNNNNNNNGG	2 cut(s)	734, 1058
BsnI	GGCC	2 cut(s)	759, 1025
Bsp1286I	GDGCHC	1 cut(s)	495
Bsp143I	GATC	1 cut(s)	520
BspANI	GGCC	2 cut(s)	759, 1025
BspCNI	CTCAG	2 cut(s)	307, 539
BspLI	GGNNCC	2 cut(s)	522, 666
BspMAI	CTGCAG	1 cut(s)	801
BspPI	GGATC	2 cut(s)	515, 528
BsrBI	CCGCTC	1 cut(s)	59
BsrDI	GCAATG	1 cut(s)	1092
BsrI	ACTGG	6 cut(s)	255, 291, 782, 962, 1052, 1135
BssECI	CCNNGG	2 cut(s)	477, 560
BssMI	GATC	1 cut(s)	520
Bst2UI	CCWGG	2 cut(s)	930, 1059
Bst4CI	ACNGT	1 cut(s)	817
Bst6I	CTCTTC	2 cut(s)	178, 846
BstBAI	YACGTR	1 cut(s)	80
BstC8I	GCNNGC	3 cut(s)	897, 984, 988
BstDEI	CTNAG	2 cut(s)	315, 526
BstDSI	CCRYGG	1 cut(s)	477
BstENI	CCTNNNNNAGG	1 cut(s)	732
BstF5I	GGATG	9 cut(s)	138, 336, 343, 478, 537, 577, 622, 723, 783
BstKTI	GATC	1 cut(s)	523
BstMBI	GATC	1 cut(s)	520
BstMWI	GCNNNNNNNGC	1 cut(s)	556
BstNI	CCWGG	2 cut(s)	930, 1059
BstSCI	CCNGG	3 cut(s)	355, 928, 1057
BstSFI	CTRYAG	4 cut(s)	797, 813, 888, 1080
BstV1I	GCAGC	5 cut(s)	20, 20, 683, 783, 896
BstX2I	RGATCY	1 cut(s)	520
BstYI	RGATCY	1 cut(s)	520
BsuI	GTATCC	2 cut(s)	235, 1116
BsuRI	GGCC	2 cut(s)	759, 1025
BtgI	CCRYGG	1 cut(s)	477
BtsCI	GGATG	9 cut(s)	138, 336, 343, 478, 537, 577, 622, 723, 783
Cac8I	GCNNGC	3 cut(s)	897, 984, 988
Cfr13I	GGNCC	1 cut(s)	1070
CsiI	ACCWGGT	1 cut(s)	928
Csp6I	GTAC	3 cut(s)	81, 684, 1055
CviAII	CATG	2 cut(s)	533, 652
CviQI	GTAC	3 cut(s)	81, 684, 1055
DdeI	CTNAG	2 cut(s)	315, 526
DpnI	GATC	1 cut(s)	522
DpnII	GATC	1 cut(s)	520
DraI	TTTAAA	1 cut(s)	588
EaeI	YGGCCR	1 cut(s)	757
Eam1104I	CTCTTC	2 cut(s)	178, 846
EarI	CTCTTC	2 cut(s)	178, 846
Eco47I	GGWCC	1 cut(s)	1070
Eco57I	CTGAAG	1 cut(s)	1023
EcoNI	CCTNNNNNAGG	1 cut(s)	732
EcoRII	CCWGG	2 cut(s)	928, 1057
FaeI	CATG	2 cut(s)	536, 655
FatI	CATG	2 cut(s)	532, 651
FauI	CCCGC	3 cut(s)	137, 636, 979
FauNDI	CATATG	1 cut(s)	567
Fnu4HI	GCNGC	9 cut(s)	6, 9, 34, 54, 57, 133, 697, 797, 885
FokI	GGATG	9 cut(s)	125, 343, 350, 485, 524, 584, 629, 710, 790
Fsp4HI	GCNGC	9 cut(s)	6, 9, 34, 54, 57, 133, 697, 797, 885
FspBI	CTAG	1 cut(s)	998
GluI	GCNGC	9 cut(s)	6, 9, 34, 54, 57, 133, 697, 797, 885
GsuI	CTGGAG	1 cut(s)	1035
HaeIII	GGCC	2 cut(s)	759, 1025
HapII	CCGG	4 cut(s)	356, 467, 507, 760
Hin1II	CATG	2 cut(s)	536, 655
HincII	GTYRAC	1 cut(s)	858
HindII	GTYRAC	1 cut(s)	858
HinfI	GANTC	4 cut(s)	392, 497, 844, 954
HpaI	GTTAAC	1 cut(s)	858
HpaII	CCGG	4 cut(s)	356, 467, 507, 760
HphI	GGTGA	2 cut(s)	617, 919
Hpy166II	GTNNAC	2 cut(s)	198, 858
Hpy188I	TCNGA	2 cut(s)	241, 953
Hpy188III	TCNNGA	2 cut(s)	834, 848
Hpy8I	GTNNAC	2 cut(s)	198, 858
Hpy99I	CGWCG	2 cut(s)	16, 122
HpyAV	CCTTC	4 cut(s)	82, 328, 412, 679
HpyCH4III	ACNGT	1 cut(s)	817
HpyCH4IV	ACGT	1 cut(s)	79
HpyCH4V	TGCA	6 cut(s)	341, 460, 699, 712, 799, 935
HpyF10VI	GCNNNNNNNGC	1 cut(s)	556
HpyF3I	CTNAG	2 cut(s)	315, 526
HpySE526I	ACGT	1 cut(s)	79
Hsp92II	CATG	2 cut(s)	536, 655
KspAI	GTTAAC	1 cut(s)	858
Kzo9I	GATC	1 cut(s)	520
LmnI	GCTCC	2 cut(s)	41, 762
Lsp1109I	GCAGC	5 cut(s)	20, 20, 683, 783, 896
LweI	GCATC	5 cut(s)	328, 463, 546, 768, 799
MabI	ACCWGGT	1 cut(s)	928
MaeI	CTAG	1 cut(s)	998
MaeII	ACGT	1 cut(s)	79
MalI	GATC	1 cut(s)	522
MbiI	CCGCTC	1 cut(s)	59
MboI	GATC	1 cut(s)	520
MboII	GAAGA	8 cut(s)	165, 168, 171, 292, 401, 427, 818, 863
MfeI	CAATTG	3 cut(s)	191, 207, 342
MflI	RGATCY	1 cut(s)	520
MhlI	GDGCHC	1 cut(s)	495
MluCI	AATT	7 cut(s)	191, 207, 297, 342, 753, 1032, 1044
MlyI	GAGTC	1 cut(s)	491
MmeI	TCCRAC	3 cut(s)	358, 931, 982
MseI	TTAA	3 cut(s)	587, 857, 924
MslI	CAYNNNNRTG	2 cut(s)	570, 717
MspA1I	CMGCKG	1 cut(s)	887
MspI	CCGG	4 cut(s)	356, 467, 507, 760
MspR9I	CCNGG	3 cut(s)	357, 930, 1059
MunI	CAATTG	3 cut(s)	191, 207, 342
MvaI	CCWGG	2 cut(s)	930, 1059
MwoI	GCNNNNNNNGC	1 cut(s)	556
NciI	CCSGG	1 cut(s)	357
NdeI	CATATG	1 cut(s)	567
NdeII	GATC	1 cut(s)	520
NlaIII	CATG	2 cut(s)	536, 655
NlaIV	GGNNCC	2 cut(s)	522, 666
NmeAIII	GCCGAG	1 cut(s)	541
PfeI	GAWTC	3 cut(s)	392, 844, 954
PkrI	GCNGC	9 cut(s)	7, 10, 35, 55, 58, 134, 698, 798, 886
PleI	GAGTC	1 cut(s)	491
PpsI	GAGTC	1 cut(s)	491
Ppu21I	YACGTR	1 cut(s)	80
PsiI	TTATAA	1 cut(s)	1017
Psp6I	CCWGG	2 cut(s)	928, 1057
PspGI	CCWGG	2 cut(s)	928, 1057
PspN4I	GGNNCC	2 cut(s)	522, 666
PspPI	GGNCC	1 cut(s)	1070
PstI	CTGCAG	1 cut(s)	801
PsuI	RGATCY	1 cut(s)	520
PvuII	CAGCTG	1 cut(s)	887
RsaI	GTAC	3 cut(s)	82, 685, 1056
RsaNI	GTAC	3 cut(s)	81, 684, 1055
RseI	CAYNNNNRTG	2 cut(s)	570, 717
SaqAI	TTAA	3 cut(s)	587, 857, 924
SatI	GCNGC	9 cut(s)	6, 9, 34, 54, 57, 133, 697, 797, 885
Sau3AI	GATC	1 cut(s)	520
Sau96I	GGNCC	1 cut(s)	1070
SchI	GAGTC	1 cut(s)	491
ScrFI	CCNGG	3 cut(s)	357, 930, 1059
SduI	GDGCHC	1 cut(s)	495
SexAI	ACCWGGT	1 cut(s)	928
SfaNI	GCATC	5 cut(s)	328, 463, 546, 768, 799
SfcI	CTRYAG	4 cut(s)	797, 813, 888, 1080
SinI	GGWCC	1 cut(s)	1070
SmiMI	CAYNNNNRTG	2 cut(s)	570, 717
Sse9I	AATT	7 cut(s)	191, 207, 297, 342, 753, 1032, 1044
SspI	AATATT	2 cut(s)	637, 769
SspMI	CTAG	1 cut(s)	998
StyD4I	CCNGG	3 cut(s)	355, 928, 1057
TaaI	ACNGT	1 cut(s)	817
TaiI	ACGT	1 cut(s)	82
TaqI	TCGA	2 cut(s)	14, 495
TasI	AATT	7 cut(s)	191, 207, 297, 342, 753, 1032, 1044
TatI	WGTACW	1 cut(s)	683
TauI	GCSGC	4 cut(s)	8, 56, 59, 135
TfiI	GAWTC	3 cut(s)	392, 844, 954
Tru1I	TTAA	3 cut(s)	587, 857, 924
Tru9I	TTAA	3 cut(s)	587, 857, 924
TseI	GCWGC	5 cut(s)	8, 33, 696, 796, 884
TspDTI	ATGAA	3 cut(s)	347, 521, 588
TspGWI	ACGGA	2 cut(s)	41, 494
VpaK11BI	GGWCC	1 cut(s)	1070
XagI	CCTNNNNNAGG	1 cut(s)	732
XspI	CTAG	1 cut(s)	998

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.