AT4G02733

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0004316
Physical Location & Seq
Reverse (-)
1210140 .. 1211532
1393 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G02733.1

Sequence Viewer

Length: 906 bp
ATGTTGTCCACCGAAAACCCTAACTCTAGTCCCCTATCTGATTCTCTAGAATTCAATGAAAGGCACTTGGATTCAATAATCTCCTCCTTGGTTACATTTCCGGATTCTCCATCTCTCTCCATAAGCTCCTCTTTCGATCGAGTACTTGATCATCTTCTCTCCTCTGGTGATGTCTCCGTCCAAGACCAACTCGTAGATCGTACTCTCGAGCGCTTCTCGCTTCTTCTCCAATCCACCAAACGTTGCTCCCAGAAACGAGCCACACTTCACAATTCCATCTCCTGGTTCCTCCCTTCGGAACTCACCGTTAAGGTATTTTCGATGGTTGATACAAAATCCCTAATGCAAGCTTCTGCATGCTGCACAATGTTCAATAATTGTGCCATGGACCCTTTATGTTACTTTCATATTGATTTGACAAAAGCATTTAAACATGTTGATGACCGCGTTTTGCGTACTTTGCTTAATCGTTCTGGAAAACAACTCAGATCCCTTAAACTTGGTCGTGTTGATGCTCCCGGATGTTTGTTTAGAAGTTCTTGTCTTCCTCCACTAATACTTTATGGGAATAATGCGAGAAGAGCCCTTAAACTTGGTCGTGATCCTCCTGGTCTTGGATCTTTGTTTACAAGATCTTGTTTTGATCCACTAAAACTTACCGGGAATCTTTTGACAAGTCTACACATTTACTCTCTTGGATTCATGAACATGAATTCCTTTTTGGATCCATTGTCAGCCTGCTCGAATCTAACCGATTTGAAGATTGTTGGAGTGAATGTTTTGTTAGAGCCGATATTAGAGTTACTTGCGAGAAACTGTTGCTTGATAGAGCATCTGTTCTTAGATAACTGTTCTCAAGGTAAAACTTTTACATGGTGGGGCTTCCTTTATTTTACTGTGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

301

Amino Acids

33.66

Weight (kDa)

7.97

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 96 - 139 8.8e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 282
AccI GTMKAC 1 cut(s) 679
AccII CGCG 1 cut(s) 447
AccIII TCCGGA 1 cut(s) 100
AciI CCGC 1 cut(s) 445
AclI AACGTT 1 cut(s) 241
AclWI GGATC 6 cut(s) 483, 596, 625, 638, 719, 732
AcsI RAATTY 2 cut(s) 50, 712
AfaI GTAC 3 cut(s) 144, 202, 457
AfeI AGCGCT 1 cut(s) 212
AfiI CCNNNNNNNGG 3 cut(s) 282, 295, 614
AflIII ACRYGT 1 cut(s) 433
AgsI TTSAA 4 cut(s) 55, 75, 373, 760
AjnI CCWGG 2 cut(s) 281, 607
AjuI GAANNNNNNNTTGG 2 cut(s) 704, 736
AloI GAACNNNNNNTCC 2 cut(s) 698, 730
AluBI AGCT 2 cut(s) 126, 350
AluI AGCT 2 cut(s) 126, 350
Alw26I GTCTC 1 cut(s) 178
AlwI GGATC 6 cut(s) 483, 596, 625, 638, 719, 732
Ama87I CYCGRG 1 cut(s) 206
Aor13HI TCCGGA 1 cut(s) 100
Aor51HI AGCGCT 1 cut(s) 212
ApeKI GCWGC 1 cut(s) 360
ApoI RAATTY 2 cut(s) 50, 712
AspLEI GCGC 1 cut(s) 213
AspS9I GGNCC 1 cut(s) 388
AsuC2I CCSGG 2 cut(s) 519, 661
AsuHPI GGTGA 2 cut(s) 179, 295
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 1 cut(s) 388
BamHI GGATCC 1 cut(s) 724
BanII GRGCYC 1 cut(s) 586
BbsI GAAGAC 1 cut(s) 536
BbvI GCAGC 1 cut(s) 347
BccI CCATC 3 cut(s) 118, 284, 316
BciT130I CCWGG 2 cut(s) 283, 609
BclI TGATCA 1 cut(s) 148
BcnI CCSGG 2 cut(s) 519, 661
BcoDI GTCTC 1 cut(s) 178
BfaI CTAG 2 cut(s) 27, 47
BfoI RGCGCY 1 cut(s) 214
BglII AGATCT 1 cut(s) 632
BisI GCNGC 1 cut(s) 361
BlsI GCNGC 1 cut(s) 362
BmcAI AGTACT 1 cut(s) 144
Bme1390I CCNGG 4 cut(s) 283, 519, 609, 661
Bme18I GGWCC 1 cut(s) 388
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 1 cut(s) 388
BmiI GGNNCC 3 cut(s) 287, 390, 726
BmrFI CCNGG 4 cut(s) 283, 519, 609, 661
BmsI GCATC 2 cut(s) 502, 841
BpiI GAAGAC 1 cut(s) 536
BplI GAGNNNNNCTC 2 cut(s) 200, 232
BpuEI CTTGAG 1 cut(s) 840
BpuMI CCSGG 2 cut(s) 519, 661
BsaJI CCNNGG 2 cut(s) 87, 384
BsaWI WCCGGW 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 762, 792
Bsc4I CCNNNNNNNGG 3 cut(s) 282, 295, 614
BseAI TCCGGA 1 cut(s) 100
BseBI CCWGG 2 cut(s) 283, 609
BseDI CCNNGG 2 cut(s) 87, 384
BseGI GGATG 1 cut(s) 527
BseLI CCNNNNNNNGG 3 cut(s) 282, 295, 614
BseMII CTCAG 1 cut(s) 499
BseRI GAGGAG 3 cut(s) 73, 118, 151
BseXI GCAGC 1 cut(s) 347
BsgI GTGCAG 1 cut(s) 346
Bsh1236I CGCG 1 cut(s) 447
Bsh1285I CGRYCG 1 cut(s) 139
BsiEI CGRYCG 1 cut(s) 139
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 3 cut(s) 101, 519, 660
BslFI GGGAC 1 cut(s) 15
BslI CCNNNNNNNGG 3 cut(s) 282, 295, 614
BsmAI GTCTC 1 cut(s) 178
BsmFI GGGAC 1 cut(s) 15
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 1 cut(s) 586
Bsp13I TCCGGA 1 cut(s) 100
Bsp143I GATC 9 cut(s) 136, 148, 196, 488, 601, 617, 632, 643, 724
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 1 cut(s) 445
BspCNI CTCAG 1 cut(s) 498
BspEI TCCGGA 1 cut(s) 100
BspFNI CGCG 1 cut(s) 447
BspHI TCATGA 1 cut(s) 702
BspLI GGNNCC 3 cut(s) 287, 390, 726
BspPI GGATC 6 cut(s) 483, 596, 625, 638, 719, 732
BspQI GCTCTTC 1 cut(s) 574
BssECI CCNNGG 2 cut(s) 87, 384
BssMI GATC 9 cut(s) 136, 148, 196, 488, 601, 617, 632, 643, 724
BssT1I CCWWGG 2 cut(s) 87, 384
Bst2UI CCWGG 2 cut(s) 283, 609
Bst4CI ACNGT 4 cut(s) 307, 818, 851, 898
Bst6I CTCTTC 1 cut(s) 574
BstC8I GCNNGC 3 cut(s) 348, 358, 739
BstDEI CTNAG 2 cut(s) 485, 841
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 1 cut(s) 527
BstFNI CGCG 1 cut(s) 447
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 1 cut(s) 213
BstKTI GATC 9 cut(s) 139, 151, 199, 491, 604, 620, 635, 646, 727
BstMAI GTCTC 1 cut(s) 178
BstMBI GATC 9 cut(s) 136, 148, 196, 488, 601, 617, 632, 643, 724
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 3 cut(s) 217, 460, 581
BstNI CCWGG 2 cut(s) 283, 609
BstNSI RCATGY 2 cut(s) 360, 437
BstSCI CCNGG 4 cut(s) 281, 517, 607, 659
BstUI CGCG 1 cut(s) 447
BstV1I GCAGC 1 cut(s) 347
BstV2I GAAGAC 1 cut(s) 536
BstX2I RGATCY 4 cut(s) 488, 617, 632, 724
BstYI RGATCY 4 cut(s) 488, 617, 632, 724
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 1 cut(s) 527
Cac8I GCNNGC 3 cut(s) 348, 358, 739
CciI TCATGA 1 cut(s) 702
CfoI GCGC 1 cut(s) 213
Cfr13I GGNCC 1 cut(s) 388
Csp6I GTAC 3 cut(s) 143, 201, 456
CviAII CATG 6 cut(s) 357, 385, 434, 703, 709, 873
CviJI RGCY 8 cut(s) 126, 260, 350, 584, 737, 790, 882, 902
CviKI_1 RGCY 8 cut(s) 126, 260, 350, 584, 737, 790, 882, 902
CviQI GTAC 3 cut(s) 143, 201, 456
DdeI CTNAG 2 cut(s) 485, 841
DpnI GATC 9 cut(s) 138, 150, 198, 490, 603, 619, 634, 645, 726
DpnII GATC 9 cut(s) 136, 148, 196, 488, 601, 617, 632, 643, 724
DraI TTTAAA 1 cut(s) 430
Eam1104I CTCTTC 1 cut(s) 574
EarI CTCTTC 1 cut(s) 574
Eco130I CCWWGG 2 cut(s) 87, 384
Eco24I GRGCYC 1 cut(s) 586
Eco47I GGWCC 1 cut(s) 388
Eco47III AGCGCT 1 cut(s) 212
Eco88I CYCGRG 1 cut(s) 206
EcoRI GAATTC 2 cut(s) 50, 712
EcoRII CCWGG 2 cut(s) 281, 607
EcoT14I CCWWGG 2 cut(s) 87, 384
EcoT38I GRGCYC 1 cut(s) 586
ErhI CCWWGG 2 cut(s) 87, 384
FaeI CATG 6 cut(s) 360, 388, 437, 706, 712, 876
FalI AAGNNNNNCTT 2 cut(s) 115, 147
FaqI GGGAC 1 cut(s) 15
FatI CATG 6 cut(s) 356, 384, 433, 702, 708, 872
FbaI TGATCA 1 cut(s) 148
FblI GTMKAC 1 cut(s) 679
Fnu4HI GCNGC 1 cut(s) 361
FokI GGATG 1 cut(s) 534
FriOI GRGCYC 1 cut(s) 586
Fsp4HI GCNGC 1 cut(s) 361
FspBI CTAG 2 cut(s) 27, 47
GlaI GCGC 1 cut(s) 212
GluI GCNGC 1 cut(s) 361
HaeII RGCGCY 1 cut(s) 214
HapII CCGG 3 cut(s) 101, 519, 660
HhaI GCGC 1 cut(s) 213
Hin1II CATG 6 cut(s) 360, 388, 437, 706, 712, 876
Hin6I GCGC 1 cut(s) 211
HinP1I GCGC 1 cut(s) 211
HindIII AAGCTT 1 cut(s) 348
HinfI GANTC 6 cut(s) 41, 71, 104, 664, 699, 745
HpaII CCGG 3 cut(s) 101, 519, 660
HphI GGTGA 2 cut(s) 179, 295
Hpy166II GTNNAC 3 cut(s) 9, 627, 680
Hpy188I TCNGA 3 cut(s) 40, 298, 488
Hpy188III TCNNGA 6 cut(s) 47, 101, 206, 474, 599, 703
Hpy8I GTNNAC 3 cut(s) 9, 627, 680
HpyAV CCTTC 1 cut(s) 303
HpyCH4III ACNGT 4 cut(s) 307, 818, 851, 898
HpyCH4IV ACGT 1 cut(s) 241
HpyCH4V TGCA 3 cut(s) 346, 356, 363
HpyF10VI GCNNNNNNNGC 3 cut(s) 217, 460, 581
HpyF3I CTNAG 2 cut(s) 485, 841
HpySE526I ACGT 1 cut(s) 241
Hsp92II CATG 6 cut(s) 360, 388, 437, 706, 712, 876
HspAI GCGC 1 cut(s) 211
Kpn2I TCCGGA 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 9 cut(s) 136, 148, 196, 488, 601, 617, 632, 643, 724
LguI GCTCTTC 1 cut(s) 574
LmnI GCTCC 3 cut(s) 131, 251, 520
Lsp1109I GCAGC 1 cut(s) 347
LweI GCATC 2 cut(s) 502, 841
MaeI CTAG 2 cut(s) 27, 47
MaeII ACGT 1 cut(s) 241
MaeIII GTNAC 3 cut(s) 91, 398, 801
MalI GATC 9 cut(s) 138, 150, 198, 490, 603, 619, 634, 645, 726
MboI GATC 9 cut(s) 136, 148, 196, 488, 601, 617, 632, 643, 724
MboII GAAGA 5 cut(s) 146, 215, 536, 591, 772
MflI RGATCY 4 cut(s) 488, 617, 632, 724
MhlI GDGCHC 1 cut(s) 586
MluCI AATT 4 cut(s) 50, 271, 376, 712
MmeI TCCRAC 1 cut(s) 748
MnlI CCTC 6 cut(s) 94, 139, 172, 299, 558, 615
MroI TCCGGA 1 cut(s) 100
MseI TTAA 6 cut(s) 309, 429, 465, 495, 588, 904
MslI CAYNNNNRTG 2 cut(s) 438, 707
MspI CCGG 3 cut(s) 101, 519, 660
MspR9I CCNGG 4 cut(s) 283, 519, 609, 661
MvaI CCWGG 2 cut(s) 283, 609
MvnI CGCG 1 cut(s) 447
MwoI GCNNNNNNNGC 3 cut(s) 217, 460, 581
NciI CCSGG 2 cut(s) 519, 661
NcoI CCATGG 1 cut(s) 384
NdeII GATC 9 cut(s) 136, 148, 196, 488, 601, 617, 632, 643, 724
NlaIII CATG 6 cut(s) 360, 388, 437, 706, 712, 876
NlaIV GGNNCC 3 cut(s) 287, 390, 726
NspI RCATGY 2 cut(s) 360, 437
PaeI GCATGC 1 cut(s) 360
PaeR7I CTCGAG 1 cut(s) 206
PagI TCATGA 1 cut(s) 702
PciI ACATGT 1 cut(s) 433
PciSI GCTCTTC 1 cut(s) 574
PfeI GAWTC 6 cut(s) 41, 71, 104, 664, 699, 745
PflMI CCANNNNNTGG 1 cut(s) 282
PfoI TCCNGGA 1 cut(s) 517
PkrI GCNGC 1 cut(s) 362
Ple19I CGATCG 1 cut(s) 139
PscI ACATGT 1 cut(s) 433
Psp1406I AACGTT 1 cut(s) 241
Psp6I CCWGG 2 cut(s) 281, 607
PspGI CCWGG 2 cut(s) 281, 607
PspN4I GGNNCC 3 cut(s) 287, 390, 726
PspPI GGNCC 1 cut(s) 388
PsuI RGATCY 4 cut(s) 488, 617, 632, 724
PvuI CGATCG 1 cut(s) 139
RsaI GTAC 3 cut(s) 144, 202, 457
RsaNI GTAC 3 cut(s) 143, 201, 456
RseI CAYNNNNRTG 2 cut(s) 438, 707
SapI GCTCTTC 1 cut(s) 574
SaqAI TTAA 6 cut(s) 309, 429, 465, 495, 588, 904
SatI GCNGC 1 cut(s) 361
Sau3AI GATC 9 cut(s) 136, 148, 196, 488, 601, 617, 632, 643, 724
Sau96I GGNCC 1 cut(s) 388
ScaI AGTACT 1 cut(s) 144
ScrFI CCNGG 4 cut(s) 283, 519, 609, 661
SduI GDGCHC 1 cut(s) 586
SetI ASST 5 cut(s) 128, 244, 315, 352, 862
SfaNI GCATC 2 cut(s) 502, 841
Sfr274I CTCGAG 1 cut(s) 206
SinI GGWCC 1 cut(s) 388
SlaI CTCGAG 1 cut(s) 206
SmiMI CAYNNNNRTG 2 cut(s) 438, 707
SmlI CTYRAG 2 cut(s) 206, 855
SmoI CTYRAG 2 cut(s) 206, 855
SphI GCATGC 1 cut(s) 360
Sse9I AATT 4 cut(s) 50, 271, 376, 712
SsiI CCGC 1 cut(s) 445
SspMI CTAG 2 cut(s) 27, 47
StyD4I CCNGG 4 cut(s) 281, 517, 607, 659
StyI CCWWGG 2 cut(s) 87, 384
TaaI ACNGT 4 cut(s) 307, 818, 851, 898
TaiI ACGT 1 cut(s) 244
TaqI TCGA 5 cut(s) 135, 139, 207, 320, 743
TasI AATT 4 cut(s) 50, 271, 376, 712
TatI WGTACW 1 cut(s) 142
TfiI GAWTC 6 cut(s) 41, 71, 104, 664, 699, 745
Tru1I TTAA 6 cut(s) 309, 429, 465, 495, 588, 904
Tru9I TTAA 6 cut(s) 309, 429, 465, 495, 588, 904
TseI GCWGC 1 cut(s) 360
TspDTI ATGAA 5 cut(s) 72, 395, 691, 719, 725
TspGWI ACGGA 1 cut(s) 166
Van91I CCANNNNNTGG 1 cut(s) 282
VpaK11BI GGWCC 1 cut(s) 388
XapI RAATTY 2 cut(s) 50, 712
XbaI TCTAGA 1 cut(s) 46
XceI RCATGY 2 cut(s) 360, 437
XhoI CTCGAG 1 cut(s) 206
XmiI GTMKAC 1 cut(s) 679
XspI CTAG 2 cut(s) 27, 47
ZrmI AGTACT 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.