MD03G1263100.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004316
Physical Location & Seq
Forward (+)
34897285 .. 34901503
4219 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1263100.v1.1.491

Sequence Viewer

Length: 1581 bp
ATGGATACCCTGCTGCACAGCCCGTCATCCATGGCGAAGCGACCCTGCCCCTCCCAAAACCCTAGAACCCCAAACCTCAACCAGCTCGATCGTGACCGCCTCCTCGACACATTTCTCGAGTTCTCCGACTCCCCGCCCTTCGCCATCGACCTCTCCTTCGAGCGCCTCCTCGAGTCACGGGCCTCCGACGCCGATCAGACCAAGCTCATCGATCGCGCCCTCCAATTGGGCTCCGTCCTCCTCGAGGCCGCCAAGCGCTCCGCCAGGAAGCGCGCCTCCAAGCACAACTCGCTCGCCTGGGTTCTCCCTCCGGACCTCACTATCAAGGTCTTTTCCATGCTTGATACACAAAGCCTATGCTATGCTGCTGCTACTTGTTCAATGTTTAATAAGTGTGCCATGGATTCCTCTTGCTACGCCAATATTGACTTGACAACAGTTGTCCCAAAAGTTAACAATGCGGTAGTTTCCACAATGATTCACCGAGCAGGGAAATCTCTCCAGTCTCTTAAGCTTGGGGTAGTTCCAGGCCCAACTACGCCAATTGGATCTTGTCAACCATTAGTTTATACCATCAGGAATTCTGTAGATGTATCCAACTTTTCATGGAATGATAAGAGATCCCGACAAGGAAAGGAGTCATCGATTCTTAGTAGGTCCTGTTTAAGCCCTTTAGGCGCGAACAATGGTGCTCCAGGGATTCTTGTGAGAAGGTTGCACCTTTACAATATTGAAAGAATGGATAATACGTCACTCTCTGGGGCACTAGGTGCTTGCCCATTTCTTGCTGATTTGGAGATTGTTGGCCTTCATGTTGAATTGAGGCAAACACTGCTGTCAGTGAGTGCAAACTGTCAATCAATAGAGCGTCTGTTCTTTGAATCTTCAAAATCAGGTAGAGATGACAGTTTGAAATCACCAACTTGTGTTGATCTGGTGAACAATTGCCCTCATCTAACATCATTGGCCCTCAGAGGGTTTAAGTTACATGATTATAAAGTTCGTATACTTCTTAAGGGATTTAAGAAATTAAAGTATGTTGATTTTTCGACTTCCTACTCGATCACCGGATCATTTTTAAGGAACCTTGGAAGCAGCACAGGTGGGAATCTGCTGGAAGTCTTGATTCTACGCGATTGCATGCATCTCAAAATGGTGGAGGCGACTCGGTTGTTGACAGCTGTTCTTGCTGGGGATTTCAAGTTCCTTAGACATCTTGATATATCTAACAGAGAAGGTTTGGCATCTGAGGATGACTGGATTCAGAGATGCCACACCTTAAGCACCATTCCCTTGAATCGGGTGTTAGAAGAAAGGCCTAATCTCTGTCTGCTGGCTGAGCTTCCACCAGAAGGAAGTTACCCTGACATGGACCCAATGTTTGACAGTGAGCCATATAGTGATATCAGTCTGCCATCGGCATCGCAGATGAGCAGTCCTTTATCTGATGGTTCAATGTTTGTGAGTTTTTCTGAGAGCAGTTACAATAGCGATCATGGTAGTGGCAACGAGGATGCTCGAGATGTTGGTTATGTAATTTATGATGAAAGCTCAGACGAGGTGGACTACGTGGCCGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box-like

Protein Analysis

527

Amino Acids

57.99

Weight (kDa)

5.93

Isoelectric Point (pI)

50.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 101 - 144 9.1e-08 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 996
AbsI CCTCGAGG 1 cut(s) 242
AccI GTMKAC 1 cut(s) 1006
AccII CGCG 4 cut(s) 216, 273, 680, 1134
AccIII TCCGGA 1 cut(s) 310
AciI CCGC 5 cut(s) 97, 134, 249, 261, 461
AclWI GGATC 3 cut(s) 556, 615, 1078
AcoI YGGCCR 1 cut(s) 1572
AcsI RAATTY 1 cut(s) 580
AcyI GRCGYC 1 cut(s) 189
AdeI CACNNNGTG 1 cut(s) 770
AfeI AGCGCT 1 cut(s) 257
AfiI CCNNNNNNNGG 6 cut(s) 226, 244, 975, 1299, 1352, 1369
AflII CTTAAG 3 cut(s) 509, 1013, 1279
AgsI TTSAA 9 cut(s) 381, 734, 818, 881, 888, 913, 1201, 1297, 1455
AjnI CCWGG 4 cut(s) 263, 296, 526, 694
AloI GAACNNNNNNTCC 2 cut(s) 1187, 1219
AluBI AGCT 6 cut(s) 85, 205, 514, 1181, 1342, 1551
AluI AGCT 6 cut(s) 85, 205, 514, 1181, 1342, 1551
Alw21I GWGCWC 1 cut(s) 694
Alw26I GTCTC 1 cut(s) 510
AlwI GGATC 3 cut(s) 556, 615, 1078
Ama87I CYCGRG 4 cut(s) 116, 170, 242, 1518
Aor13HI TCCGGA 1 cut(s) 310
Aor51HI AGCGCT 1 cut(s) 257
AoxI GGCC 7 cut(s) 180, 246, 529, 805, 966, 1316, 1572
ApeKI GCWGC 4 cut(s) 13, 365, 368, 1095
ApoI RAATTY 1 cut(s) 580
ArsI GACNNNNNNTTYG 4 cut(s) 140, 172, 1364, 1396
AspLEI GCGC 6 cut(s) 165, 218, 258, 273, 275, 680
AspS9I GGNCC 6 cut(s) 180, 313, 530, 657, 967, 1372
AsuHPI GGTGA 4 cut(s) 473, 909, 949, 1057
AvaI CYCGRG 4 cut(s) 116, 170, 242, 1518
AvaII GGWCC 3 cut(s) 313, 657, 1372
BaeGI GKGCMC 1 cut(s) 766
BanII GRGCYC 1 cut(s) 233
Bbv12I GWGCWC 1 cut(s) 694
BbvI GCAGC 3 cut(s) 352, 355, 1107
BccI CCATC 4 cut(s) 152, 581, 1423, 1442
BceAI ACGGC 1 cut(s) 1559
BciT130I CCWGG 4 cut(s) 265, 298, 528, 696
BciVI GTATCC 1 cut(s) 604
BcoDI GTCTC 1 cut(s) 510
BfaI CTAG 2 cut(s) 63, 767
BfmI CTRYAG 1 cut(s) 585
BfoI RGCGCY 2 cut(s) 166, 259
BfrI CTTAAG 3 cut(s) 509, 1013, 1279
BfuI GTATCC 1 cut(s) 604
BglI GCCNNNNNGGC 1 cut(s) 675
BisI GCNGC 5 cut(s) 14, 249, 366, 369, 1096
BlpI GCTNAGC 1 cut(s) 1338
BlsI GCNGC 5 cut(s) 15, 250, 367, 370, 1097
Bme1390I CCNGG 4 cut(s) 265, 298, 528, 696
Bme18I GGWCC 3 cut(s) 313, 657, 1372
BmeT110I CYCGRG 4 cut(s) 116, 170, 242, 1518
BmgT120I GGNCC 6 cut(s) 180, 313, 530, 657, 967, 1372
BmiI GGNNCC 3 cut(s) 232, 1085, 1374
BmrFI CCNGG 4 cut(s) 265, 298, 528, 696
BmsI GCATC 5 cut(s) 1153, 1253, 1259, 1430, 1504
BpmI CTGGAG 2 cut(s) 485, 678
Bpu1102I GCTNAGC 1 cut(s) 1338
Bsa29I ATCGAT 2 cut(s) 210, 644
BsaAI YACGTR 1 cut(s) 1570
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 5 cut(s) 30, 297, 399, 695, 1087
BsaWI WCCGGW 2 cut(s) 310, 1067
Bsc4I CCNNNNNNNGG 6 cut(s) 226, 244, 975, 1299, 1352, 1369
Bse1I ACTGG 2 cut(s) 502, 1262
BseAI TCCGGA 1 cut(s) 310
BseBI CCWGG 4 cut(s) 265, 298, 528, 696
BseCI ATCGAT 2 cut(s) 210, 644
BseDI CCNNGG 5 cut(s) 30, 297, 399, 695, 1087
BseGI GGATG 3 cut(s) 26, 1258, 1519
BseLI CCNNNNNNNGG 6 cut(s) 226, 244, 975, 1299, 1352, 1369
BseMII CTCAG 5 cut(s) 985, 1239, 1329, 1464, 1566
BseNI ACTGG 2 cut(s) 502, 1262
BsePI GCGCGC 1 cut(s) 271
BseRI GAGGAG 3 cut(s) 92, 158, 230
BseSI GKGCMC 1 cut(s) 766
BseXI GCAGC 3 cut(s) 352, 355, 1107
BseYI CCCAGC 1 cut(s) 1190
Bsh1236I CGCG 4 cut(s) 216, 273, 680, 1134
Bsh1285I CGRYCG 2 cut(s) 91, 214
BshFI GGCC 7 cut(s) 182, 248, 531, 807, 968, 1318, 1574
BshVI ATCGAT 2 cut(s) 210, 644
BsiEI CGRYCG 2 cut(s) 91, 214
BsiHKAI GWGCWC 1 cut(s) 694
BsiHKCI CYCGRG 4 cut(s) 116, 170, 242, 1518
BsiSI CCGG 2 cut(s) 311, 1068
BslFI GGGAC 1 cut(s) 428
BslI CCNNNNNNNGG 6 cut(s) 226, 244, 975, 1299, 1352, 1369
BsmAI GTCTC 1 cut(s) 510
BsmFI GGGAC 1 cut(s) 428
BsnI GGCC 7 cut(s) 182, 248, 531, 807, 968, 1318, 1574
BsoBI CYCGRG 4 cut(s) 116, 170, 242, 1518
Bsp1286I GDGCHC 3 cut(s) 233, 694, 766
Bsp13I TCCGGA 1 cut(s) 310
Bsp143I GATC 9 cut(s) 88, 193, 211, 548, 620, 931, 1062, 1070, 1492
Bsp1720I GCTNAGC 1 cut(s) 1338
Bsp19I CCATGG 2 cut(s) 30, 399
BspACI CCGC 5 cut(s) 97, 134, 249, 261, 461
BspANI GGCC 7 cut(s) 182, 248, 531, 807, 968, 1318, 1574
BspCNI CTCAG 5 cut(s) 984, 1240, 1330, 1465, 1565
BspDI ATCGAT 2 cut(s) 210, 644
BspEI TCCGGA 1 cut(s) 310
BspFNI CGCG 4 cut(s) 216, 273, 680, 1134
BspLI GGNNCC 3 cut(s) 232, 1085, 1374
BspPI GGATC 3 cut(s) 556, 615, 1078
BspTI CTTAAG 3 cut(s) 509, 1013, 1279
BsrI ACTGG 2 cut(s) 502, 1262
BssECI CCNNGG 5 cut(s) 30, 297, 399, 695, 1087
BssHII GCGCGC 1 cut(s) 271
BssMI GATC 9 cut(s) 88, 193, 211, 548, 620, 931, 1062, 1070, 1492
BssNAI GTATAC 1 cut(s) 1007
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 3 cut(s) 30, 399, 1087
Bst1107I GTATAC 1 cut(s) 1007
Bst2UI CCWGG 4 cut(s) 265, 298, 528, 696
Bst4CI ACNGT 4 cut(s) 439, 854, 908, 1388
BstACI GRCGYC 1 cut(s) 189
BstAFI CTTAAG 3 cut(s) 509, 1013, 1279
BstAPI GCANNNNNTGC 2 cut(s) 770, 832
BstBAI YACGTR 1 cut(s) 1570
BstC8I GCNNGC 5 cut(s) 273, 294, 775, 1142, 1335
BstDEI CTNAG 7 cut(s) 650, 971, 1208, 1248, 1338, 1473, 1552
BstDSI CCRYGG 2 cut(s) 30, 399
BstENI CCTNNNNNAGG 1 cut(s) 242
BstF5I GGATG 3 cut(s) 26, 1258, 1519
BstFNI CGCG 4 cut(s) 216, 273, 680, 1134
BstH2I RGCGCY 2 cut(s) 166, 259
BstHHI GCGC 6 cut(s) 165, 218, 258, 273, 275, 680
BstKTI GATC 9 cut(s) 91, 196, 214, 551, 623, 934, 1065, 1073, 1495
BstMAI GTCTC 1 cut(s) 510
BstMBI GATC 9 cut(s) 88, 193, 211, 548, 620, 931, 1062, 1070, 1492
BstMCI CGRYCG 2 cut(s) 91, 214
BstMWI GCNNNNNNNGC 7 cut(s) 188, 289, 675, 770, 832, 1187, 1339
BstNI CCWGG 4 cut(s) 265, 298, 528, 696
BstNSI RCATGY 1 cut(s) 1144
BstSCI CCNGG 4 cut(s) 263, 296, 526, 694
BstSFI CTRYAG 1 cut(s) 585
BstSLI GKGCMC 1 cut(s) 766
BstUI CGCG 4 cut(s) 216, 273, 680, 1134
BstV1I GCAGC 3 cut(s) 352, 355, 1107
BstX2I RGATCY 2 cut(s) 548, 620
BstYI RGATCY 2 cut(s) 548, 620
BstZ17I GTATAC 1 cut(s) 1007
Bsu15I ATCGAT 2 cut(s) 210, 644
BsuI GTATCC 1 cut(s) 604
BsuRI GGCC 7 cut(s) 182, 248, 531, 807, 968, 1318, 1574
BsuTUI ATCGAT 2 cut(s) 210, 644
BtgI CCRYGG 2 cut(s) 30, 399
BtgZI GCGATG 1 cut(s) 1407
BtsCI GGATG 3 cut(s) 26, 1258, 1519
BtsI GCAGTG 1 cut(s) 830
BtsIMutI CAGTG 3 cut(s) 830, 846, 1393
Cac8I GCNNGC 5 cut(s) 273, 294, 775, 1142, 1335
CfoI GCGC 6 cut(s) 165, 218, 258, 273, 275, 680
Cfr13I GGNCC 6 cut(s) 180, 313, 530, 657, 967, 1372
ClaI ATCGAT 2 cut(s) 210, 644
CseI GACGC 2 cut(s) 197, 857
CviAII CATG 9 cut(s) 31, 337, 400, 606, 812, 989, 1141, 1369, 1496
DdeI CTNAG 7 cut(s) 650, 971, 1208, 1248, 1338, 1473, 1552
DpnI GATC 9 cut(s) 90, 195, 213, 550, 622, 933, 1064, 1072, 1494
DpnII GATC 9 cut(s) 88, 193, 211, 548, 620, 931, 1062, 1070, 1492
DraIII CACNNNGTG 1 cut(s) 770
EaeI YGGCCR 1 cut(s) 1572
EciI GGCGGA 1 cut(s) 250
Eco130I CCWWGG 3 cut(s) 30, 399, 1087
Eco147I AGGCCT 1 cut(s) 1318
Eco24I GRGCYC 1 cut(s) 233
Eco32I GATATC 1 cut(s) 1405
Eco47I GGWCC 3 cut(s) 313, 657, 1372
Eco47III AGCGCT 1 cut(s) 257
Eco88I CYCGRG 4 cut(s) 116, 170, 242, 1518
EcoNI CCTNNNNNAGG 1 cut(s) 242
EcoO109I RGGNCCY 1 cut(s) 657
EcoRI GAATTC 1 cut(s) 580
EcoRII CCWGG 4 cut(s) 263, 296, 526, 694
EcoRV GATATC 1 cut(s) 1405
EcoT14I CCWWGG 3 cut(s) 30, 399, 1087
EcoT22I ATGCAT 1 cut(s) 1146
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 3 cut(s) 30, 399, 1087
FaeI CATG 9 cut(s) 34, 340, 403, 609, 815, 992, 1144, 1372, 1499
FaqI GGGAC 1 cut(s) 428
FatI CATG 9 cut(s) 30, 336, 399, 605, 811, 988, 1140, 1368, 1495
FauI CCCGC 1 cut(s) 141
FblI GTMKAC 1 cut(s) 1006
Fnu4HI GCNGC 5 cut(s) 14, 249, 366, 369, 1096
FokI GGATG 3 cut(s) 13, 1265, 1526
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 5 cut(s) 14, 249, 366, 369, 1096
FspBI CTAG 2 cut(s) 63, 767
GlaI GCGC 6 cut(s) 164, 217, 257, 272, 274, 679
GluI GCNGC 5 cut(s) 14, 249, 366, 369, 1096
GsaI CCCAGC 1 cut(s) 1194
GsuI CTGGAG 2 cut(s) 485, 678
HaeII RGCGCY 2 cut(s) 166, 259
HaeIII GGCC 7 cut(s) 182, 248, 531, 807, 968, 1318, 1574
HapII CCGG 2 cut(s) 311, 1068
HgaI GACGC 2 cut(s) 197, 857
HhaI GCGC 6 cut(s) 165, 218, 258, 273, 275, 680
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 9 cut(s) 34, 340, 403, 609, 815, 992, 1144, 1372, 1499
Hin6I GCGC 6 cut(s) 163, 216, 256, 271, 273, 678
HinP1I GCGC 6 cut(s) 163, 216, 256, 271, 273, 678
HincII GTYRAC 3 cut(s) 454, 557, 1176
HindII GTYRAC 3 cut(s) 454, 557, 1176
HindIII AAGCTT 1 cut(s) 512
HpaI GTTAAC 1 cut(s) 454
HpaII CCGG 2 cut(s) 311, 1068
HphI GGTGA 4 cut(s) 473, 909, 949, 1057
Hpy166II GTNNAC 6 cut(s) 454, 557, 940, 1007, 1176, 1564
Hpy188I TCNGA 9 cut(s) 127, 187, 198, 974, 1249, 1266, 1447, 1474, 1555
Hpy188III TCNNGA 8 cut(s) 92, 116, 311, 577, 624, 1123, 1217, 1520
Hpy8I GTNNAC 6 cut(s) 454, 557, 940, 1007, 1176, 1564
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 6 cut(s) 148, 166, 705, 818, 1229, 1346
HpyCH4III ACNGT 4 cut(s) 439, 854, 908, 1388
HpyCH4IV ACGT 2 cut(s) 749, 1569
HpyCH4V TGCA 5 cut(s) 16, 718, 848, 1140, 1144
HpyF10VI GCNNNNNNNGC 7 cut(s) 188, 289, 675, 770, 832, 1187, 1339
HpyF3I CTNAG 7 cut(s) 650, 971, 1208, 1248, 1338, 1473, 1552
HpySE526I ACGT 2 cut(s) 749, 1569
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 9 cut(s) 34, 340, 403, 609, 815, 992, 1144, 1372, 1499
HspAI GCGC 6 cut(s) 163, 216, 256, 271, 273, 678
Kpn2I TCCGGA 1 cut(s) 310
KspAI GTTAAC 1 cut(s) 454
Kzo9I GATC 9 cut(s) 88, 193, 211, 548, 620, 931, 1062, 1070, 1492
LmnI GCTCC 3 cut(s) 236, 263, 697
Lsp1109I GCAGC 3 cut(s) 352, 355, 1107
LweI GCATC 5 cut(s) 1153, 1253, 1259, 1430, 1504
MaeI CTAG 2 cut(s) 63, 767
MaeII ACGT 2 cut(s) 749, 1569
MaeIII GTNAC 6 cut(s) 92, 174, 750, 984, 1358, 1481
MalI GATC 9 cut(s) 90, 195, 213, 550, 622, 933, 1064, 1072, 1494
MboI GATC 9 cut(s) 88, 193, 211, 548, 620, 931, 1062, 1070, 1492
MboII GAAGA 2 cut(s) 876, 1322
MfeI CAATTG 3 cut(s) 224, 543, 943
MflI RGATCY 2 cut(s) 548, 620
MhlI GDGCHC 3 cut(s) 233, 694, 766
MluCI AATT 7 cut(s) 224, 543, 580, 818, 943, 1028, 1536
MlyI GAGTC 4 cut(s) 122, 182, 647, 1159
MmeI TCCRAC 3 cut(s) 150, 210, 621
Mph1103I ATGCAT 1 cut(s) 1146
MroI TCCGGA 1 cut(s) 310
MslI CAYNNNNRTG 1 cut(s) 1500
MspA1I CMGCKG 1 cut(s) 1181
MspCI CTTAAG 3 cut(s) 509, 1013, 1279
MspI CCGG 2 cut(s) 311, 1068
MspR9I CCNGG 4 cut(s) 265, 298, 528, 696
MunI CAATTG 3 cut(s) 224, 543, 943
MvaI CCWGG 4 cut(s) 265, 298, 528, 696
MvnI CGCG 4 cut(s) 216, 273, 680, 1134
MwoI GCNNNNNNNGC 7 cut(s) 188, 289, 675, 770, 832, 1187, 1339
NcoI CCATGG 2 cut(s) 30, 399
NdeII GATC 9 cut(s) 88, 193, 211, 548, 620, 931, 1062, 1070, 1492
NlaIII CATG 9 cut(s) 34, 340, 403, 609, 815, 992, 1144, 1372, 1499
NlaIV GGNNCC 3 cut(s) 232, 1085, 1374
NmuCI GTSAC 3 cut(s) 92, 174, 750
NsiI ATGCAT 1 cut(s) 1146
NspI RCATGY 1 cut(s) 1144
PaeI GCATGC 1 cut(s) 1144
PaeR7I CTCGAG 4 cut(s) 116, 170, 242, 1518
PauI GCGCGC 1 cut(s) 271
PceI AGGCCT 1 cut(s) 1318
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 9 cut(s) 404, 478, 646, 700, 881, 1108, 1126, 1261, 1297
PkrI GCNGC 5 cut(s) 15, 250, 367, 370, 1097
Ple19I CGATCG 2 cut(s) 91, 214
PleI GAGTC 4 cut(s) 122, 181, 646, 1159
PpsI GAGTC 4 cut(s) 122, 181, 646, 1159
Ppu21I YACGTR 1 cut(s) 1570
PpuMI RGGWCCY 1 cut(s) 657
PsiI TTATAA 1 cut(s) 996
Psp5II RGGWCCY 1 cut(s) 657
Psp6I CCWGG 4 cut(s) 263, 296, 526, 694
PspFI CCCAGC 1 cut(s) 1190
PspGI CCWGG 4 cut(s) 263, 296, 526, 694
PspN4I GGNNCC 3 cut(s) 232, 1085, 1374
PspPI GGNCC 6 cut(s) 180, 313, 530, 657, 967, 1372
PspPPI RGGWCCY 1 cut(s) 657
PspXI VCTCGAGB 2 cut(s) 170, 242
PsuI RGATCY 2 cut(s) 548, 620
PteI GCGCGC 1 cut(s) 271
PvuI CGATCG 2 cut(s) 91, 214
PvuII CAGCTG 1 cut(s) 1181
RseI CAYNNNNRTG 1 cut(s) 1500
SatI GCNGC 5 cut(s) 14, 249, 366, 369, 1096
Sau3AI GATC 9 cut(s) 88, 193, 211, 548, 620, 931, 1062, 1070, 1492
Sau96I GGNCC 6 cut(s) 180, 313, 530, 657, 967, 1372
SchI GAGTC 4 cut(s) 122, 182, 647, 1159
ScrFI CCNGG 4 cut(s) 265, 298, 528, 696
SduI GDGCHC 3 cut(s) 233, 694, 766
SfaNI GCATC 5 cut(s) 1153, 1253, 1259, 1430, 1504
SfcI CTRYAG 1 cut(s) 585
Sfr274I CTCGAG 4 cut(s) 116, 170, 242, 1518
SinI GGWCC 3 cut(s) 313, 657, 1372
SlaI CTCGAG 4 cut(s) 116, 170, 242, 1518
SmiMI CAYNNNNRTG 1 cut(s) 1500
SmlI CTYRAG 7 cut(s) 116, 170, 242, 509, 1013, 1279, 1518
SmoI CTYRAG 7 cut(s) 116, 170, 242, 509, 1013, 1279, 1518
SphI GCATGC 1 cut(s) 1144
Sse9I AATT 7 cut(s) 224, 543, 580, 818, 943, 1028, 1536
SseBI AGGCCT 1 cut(s) 1318
SsiI CCGC 5 cut(s) 97, 134, 249, 261, 461
SspI AATATT 2 cut(s) 424, 730
SspMI CTAG 2 cut(s) 63, 767
StuI AGGCCT 1 cut(s) 1318
StyD4I CCNGG 4 cut(s) 263, 296, 526, 694
StyI CCWWGG 3 cut(s) 30, 399, 1087
TaaI ACNGT 4 cut(s) 439, 854, 908, 1388
TaiI ACGT 2 cut(s) 752, 1572
TasI AATT 7 cut(s) 224, 543, 580, 818, 943, 1028, 1536
TauI GCSGC 1 cut(s) 251
TfiI GAWTC 9 cut(s) 404, 478, 646, 700, 881, 1108, 1126, 1261, 1297
TscAI CASTG 3 cut(s) 837, 846, 1393
TseFI GTSAC 3 cut(s) 92, 174, 750
TseI GCWGC 4 cut(s) 13, 365, 368, 1095
Tsp45I GTSAC 3 cut(s) 92, 174, 750
TspDTI ATGAA 3 cut(s) 594, 800, 1560
TspGWI ACGGA 1 cut(s) 223
TspRI CASTG 3 cut(s) 837, 846, 1393
Vha464I CTTAAG 3 cut(s) 509, 1013, 1279
VpaK11BI GGWCC 3 cut(s) 313, 657, 1372
XagI CCTNNNNNAGG 1 cut(s) 242
XapI RAATTY 1 cut(s) 580
XceI RCATGY 1 cut(s) 1144
XhoI CTCGAG 4 cut(s) 116, 170, 242, 1518
XmiI GTMKAC 1 cut(s) 1006
XspI CTAG 2 cut(s) 63, 767
Zsp2I ATGCAT 1 cut(s) 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.