AT4G05390

ferredoxin--NADP reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006772
Physical Location & Seq
Reverse (-)
2738531 .. 2740927
2397 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G05390.1

Sequence Viewer

Length: 1137 bp
ATGGCTCTCTCAACTACTCCTTCTCAGATGTCCGTTGCACTTCCAACTAGAATTGATGGGTCGAGCCGATCTATGATTAAGGTTCAAAGTATAAGCTTCACTGATAAGTCATGGGGTCCTCCTCTGCTGAGACTAGATTCGAAATCTAGAAGCTTAGGTGTGAAAAAAAGGTCCACAATATGCATGTCACTTCAGCAATCAAGCAAATCCAAAGTCTTGGTTACTCCTCTAGAACTTGAAGATCCCAAAGAGACTCCCTTAAACTTGTTTAGGCCTAAGGAGCCTTATACTGCAACTATTGTTTCGGTTGAGAGAATTGTTGGTCCACAAGCACCTGGAGAGACTTGCCACATTGTTATTGATCATGATGGTAATGTTCCTTACTGGGAAGGACAAAGCTATGGAGTCATTCCTCCTGGTGAGAATCCCAAGAAACCTGGTGCACCTCATAACGTTCGCCTTTATTCGATTGCATCAACACGGTATGGAGATTCTTTTGATGGCAAAACAGCTAGTCTATGTGTCCGTCGAGCTATTTACTATGATCCGGAGACAGGAAAAGAAGATCCTTCCAAAGCTGGTGTATGCAGTAACTTCTTGTGCAATGCCAAACCCGGCGATAAAGTTAAAATCACCGGTCCATCTGGAAAGGTAATGCTTTTACCTGAAGATGACCCGAAAGCTACTCACATAATGATTGCTACTGGAACCGGAGTTGCTCCGTACAGAGGATACCTACGGCGTATGTTTATGGAGAATGTTCCCAATTTCAAGTTTGACGGACTTGCTTGGCTCTTCCTTGGTGTGGCTAACTCAGACAGTCTTCTCTATGATGAAGAATTTGCCGGGTACCGCAAGGACTATCCAGAAAATTTCAGGTACGACAAAGCGCTGAGCAGAGAAGAGAAAAACAAGAAAGGAGGGAAAATGTATGTGCAGGACAAGATTGAAGAATACAGCGATGAAATCTTCAAACTTCTGGACAATGGAGCTCATATTTACTTTTGCGGACTTAAAGGAATGATGCCCGGGATTCAAGATACGCTTAAGAGAGTCGCTGAAGAGCGAGGCGAAAGCTGGGAGCAGAAACTTACTCAGCTCAGGAAGAACAAGCAGTGGCATGTTGAAGTGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005507 GO:0043167 GO:0043169 GO:0046872 GO:0046914
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

378

Amino Acids

42.4

Weight (kDa)

8.77

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 232 - 346 9.2e-26 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 849
AccB1I GGYRCC 1 cut(s) 849
AccIII TCCGGA 1 cut(s) 547
AciI CCGC 2 cut(s) 853, 1008
AclI AACGTT 1 cut(s) 453
AclWI GGATC 3 cut(s) 236, 539, 560
AcsI RAATTY 2 cut(s) 839, 871
AcuI CTGAAG 3 cut(s) 176, 687, 1080
AfaI GTAC 3 cut(s) 725, 851, 881
AfeI AGCGCT 1 cut(s) 891
AfiI CCNNNNNNNGG 3 cut(s) 554, 728, 805
AflII CTTAAG 1 cut(s) 1046
AgeI ACCGGT 1 cut(s) 635
AgsI TTSAA 7 cut(s) 86, 239, 772, 950, 973, 1037, 1127
AjnI CCWGG 3 cut(s) 334, 415, 436
Alw21I GWGCWC 2 cut(s) 445, 994
Alw26I GTCTC 4 cut(s) 124, 245, 335, 545
Alw44I GTGCAC 1 cut(s) 441
AlwI GGATC 3 cut(s) 236, 539, 560
Ama87I CYCGRG 1 cut(s) 1028
Aor13HI TCCGGA 1 cut(s) 547
Aor51HI AGCGCT 1 cut(s) 891
AoxI GGCC 1 cut(s) 272
ApaLI GTGCAC 1 cut(s) 441
ApoI RAATTY 2 cut(s) 839, 871
ArsI GACNNNNNNTTYG 2 cut(s) 198, 230
AsiGI ACCGGT 1 cut(s) 635
Asp718I GGTACC 1 cut(s) 849
AspLEI GCGC 1 cut(s) 892
AspS9I GGNCC 4 cut(s) 116, 171, 323, 638
AsuC2I CCSGG 4 cut(s) 615, 847, 1029, 1030
AsuHPI GGTGA 2 cut(s) 431, 625
AsuII TTCGAA 1 cut(s) 140
AvaI CYCGRG 1 cut(s) 1028
AvaII GGWCC 4 cut(s) 116, 171, 323, 638
AxyI CCTNAGG 1 cut(s) 276
BaeGI GKGCMC 1 cut(s) 445
BanI GGYRCC 1 cut(s) 849
BanII GRGCYC 1 cut(s) 994
BbsI GAAGAC 1 cut(s) 815
Bbv12I GWGCWC 2 cut(s) 445, 994
BccI CCATC 4 cut(s) 50, 362, 494, 649
BceAI ACGGC 1 cut(s) 755
BciT130I CCWGG 3 cut(s) 336, 417, 438
BciVI GTATCC 1 cut(s) 725
BclI TGATCA 1 cut(s) 361
BcnI CCSGG 4 cut(s) 615, 847, 1029, 1030
BcoDI GTCTC 4 cut(s) 124, 245, 335, 545
BfaI CTAG 5 cut(s) 48, 134, 147, 230, 513
BfoI RGCGCY 1 cut(s) 893
BfrI CTTAAG 1 cut(s) 1046
BfuI GTATCC 1 cut(s) 725
BlpI GCTNAGC 1 cut(s) 893
Bme1390I CCNGG 7 cut(s) 336, 417, 438, 615, 847, 1029, 1030
Bme18I GGWCC 4 cut(s) 116, 171, 323, 638
BmeT110I CYCGRG 1 cut(s) 1028
BmgT120I GGNCC 4 cut(s) 116, 171, 323, 638
BmiI GGNNCC 4 cut(s) 117, 282, 709, 851
BmrFI CCNGG 7 cut(s) 336, 417, 438, 615, 847, 1029, 1030
BmrI ACTGGG 1 cut(s) 394
BmsI GCATC 2 cut(s) 482, 1014
BmuI ACTGGG 1 cut(s) 394
BpiI GAAGAC 1 cut(s) 815
BpmI CTGGAG 1 cut(s) 357
Bpu10I CCTNAGC 2 cut(s) 154, 1100
Bpu1102I GCTNAGC 1 cut(s) 893
Bpu14I TTCGAA 1 cut(s) 140
BpuMI CCSGG 4 cut(s) 615, 847, 1029, 1030
BsaJI CCNNGG 2 cut(s) 799, 1028
BsaWI WCCGGW 3 cut(s) 547, 635, 710
Bsc4I CCNNNNNNNGG 3 cut(s) 554, 728, 805
Bse118I RCCGGY 1 cut(s) 635
Bse1I ACTGG 2 cut(s) 389, 709
Bse21I CCTNAGG 1 cut(s) 276
Bse3DI GCAATG 1 cut(s) 610
BseAI TCCGGA 1 cut(s) 547
BseBI CCWGG 3 cut(s) 336, 417, 438
BseDI CCNNGG 2 cut(s) 799, 1028
BseLI CCNNNNNNNGG 3 cut(s) 554, 728, 805
BseMI GCAATG 1 cut(s) 610
BseMII CTCAG 6 cut(s) 38, 119, 828, 884, 1109, 1114
BseNI ACTGG 2 cut(s) 389, 709
BseRI GAGGAG 2 cut(s) 111, 216
BseSI GKGCMC 1 cut(s) 445
BseYI CCCAGC 1 cut(s) 1077
BsgI GTGCAG 1 cut(s) 956
BshFI GGCC 1 cut(s) 274
BshNI GGYRCC 1 cut(s) 849
BshTI ACCGGT 1 cut(s) 635
BsiHKAI GWGCWC 2 cut(s) 445, 994
BsiHKCI CYCGRG 1 cut(s) 1028
BsiSI CCGG 6 cut(s) 548, 615, 636, 711, 846, 1029
BslI CCNNNNNNNGG 3 cut(s) 554, 728, 805
BsmAI GTCTC 4 cut(s) 124, 245, 335, 545
BsnI GGCC 1 cut(s) 274
BsoBI CYCGRG 1 cut(s) 1028
Bsp119I TTCGAA 1 cut(s) 140
Bsp1286I GDGCHC 2 cut(s) 445, 994
Bsp13I TCCGGA 1 cut(s) 547
Bsp143I GATC 5 cut(s) 68, 241, 361, 544, 565
Bsp1720I GCTNAGC 1 cut(s) 893
BspACI CCGC 2 cut(s) 853, 1008
BspANI GGCC 1 cut(s) 274
BspCNI CTCAG 6 cut(s) 37, 120, 827, 885, 1108, 1113
BspEI TCCGGA 1 cut(s) 547
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 4 cut(s) 117, 282, 709, 851
BspPI GGATC 3 cut(s) 236, 539, 560
BspQI GCTCTTC 2 cut(s) 800, 1056
BspT104I TTCGAA 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 849
BspTI CTTAAG 1 cut(s) 1046
BsrDI GCAATG 1 cut(s) 610
BsrFI RCCGGY 1 cut(s) 635
BsrI ACTGG 2 cut(s) 389, 709
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 2 cut(s) 799, 1028
BssMI GATC 5 cut(s) 68, 241, 361, 544, 565
BssT1I CCWWGG 1 cut(s) 799
Bst2UI CCWGG 3 cut(s) 336, 417, 438
Bst4CI ACNGT 2 cut(s) 483, 821
Bst6I CTCTTC 3 cut(s) 800, 897, 1056
BstAFI CTTAAG 1 cut(s) 1046
BstBI TTCGAA 1 cut(s) 140
BstDEI CTNAG 8 cut(s) 24, 128, 154, 276, 814, 893, 1095, 1100
BstH2I RGCGCY 1 cut(s) 893
BstHHI GCGC 1 cut(s) 892
BstKTI GATC 5 cut(s) 71, 244, 364, 547, 568
BstMAI GTCTC 4 cut(s) 124, 245, 335, 545
BstMBI GATC 5 cut(s) 68, 241, 361, 544, 565
BstMWI GCNNNNNNNGC 1 cut(s) 280
BstNI CCWGG 3 cut(s) 336, 417, 438
BstNSI RCATGY 2 cut(s) 187, 1124
BstSCI CCNGG 7 cut(s) 334, 415, 436, 613, 845, 1027, 1028
BstSLI GKGCMC 1 cut(s) 445
BstV2I GAAGAC 1 cut(s) 815
BstX2I RGATCY 2 cut(s) 241, 565
BstXI CCANNNNNNTGG 1 cut(s) 217
BstYI RGATCY 2 cut(s) 241, 565
Bsu36I CCTNAGG 1 cut(s) 276
BsuI GTATCC 1 cut(s) 725
BsuRI GGCC 1 cut(s) 274
BtgZI GCGATG 1 cut(s) 975
BtsI GCAGTG 1 cut(s) 1121
BtsIMutI CAGTG 2 cut(s) 99, 1121
CciI TCATGA 1 cut(s) 364
CfoI GCGC 1 cut(s) 892
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 4 cut(s) 116, 171, 323, 638
Cfr9I CCCGGG 1 cut(s) 1028
CsiI ACCWGGT 1 cut(s) 436
Csp6I GTAC 3 cut(s) 724, 850, 880
CspAI ACCGGT 1 cut(s) 635
CviAII CATG 4 cut(s) 111, 184, 365, 1121
CviQI GTAC 3 cut(s) 724, 850, 880
DdeI CTNAG 8 cut(s) 24, 128, 154, 276, 814, 893, 1095, 1100
DpnI GATC 5 cut(s) 70, 243, 363, 546, 567
DpnII GATC 5 cut(s) 68, 241, 361, 544, 565
Eam1104I CTCTTC 3 cut(s) 800, 897, 1056
EarI CTCTTC 3 cut(s) 800, 897, 1056
Ecl136II GAGCTC 1 cut(s) 992
Eco130I CCWWGG 1 cut(s) 799
Eco147I AGGCCT 1 cut(s) 274
Eco24I GRGCYC 1 cut(s) 994
Eco47I GGWCC 4 cut(s) 116, 171, 323, 638
Eco47III AGCGCT 1 cut(s) 891
Eco53kI GAGCTC 1 cut(s) 992
Eco57I CTGAAG 3 cut(s) 176, 687, 1080
Eco81I CCTNAGG 1 cut(s) 276
Eco88I CYCGRG 1 cut(s) 1028
EcoICRI GAGCTC 1 cut(s) 992
EcoO109I RGGNCCY 1 cut(s) 116
EcoRII CCWGG 3 cut(s) 334, 415, 436
EcoT14I CCWWGG 1 cut(s) 799
EcoT22I ATGCAT 1 cut(s) 185
EcoT38I GRGCYC 1 cut(s) 994
ErhI CCWWGG 1 cut(s) 799
FaeI CATG 4 cut(s) 114, 187, 368, 1124
FalI AAGNNNNNCTT 2 cut(s) 1029, 1061
FatI CATG 4 cut(s) 110, 183, 364, 1120
FbaI TGATCA 1 cut(s) 361
FriOI GRGCYC 1 cut(s) 994
FspBI CTAG 5 cut(s) 48, 134, 147, 230, 513
GlaI GCGC 1 cut(s) 891
GsaI CCCAGC 1 cut(s) 1081
GsuI CTGGAG 1 cut(s) 357
HaeII RGCGCY 1 cut(s) 893
HaeIII GGCC 1 cut(s) 274
HapII CCGG 6 cut(s) 548, 615, 636, 711, 846, 1029
HhaI GCGC 1 cut(s) 892
Hin1II CATG 4 cut(s) 114, 187, 368, 1124
Hin6I GCGC 1 cut(s) 890
HinP1I GCGC 1 cut(s) 890
HindIII AAGCTT 2 cut(s) 94, 151
HinfI GANTC 7 cut(s) 137, 253, 405, 424, 491, 1033, 1053
HpaII CCGG 6 cut(s) 548, 615, 636, 711, 846, 1029
HphI GGTGA 2 cut(s) 431, 625
Hpy166II GTNNAC 3 cut(s) 174, 326, 443
Hpy188I TCNGA 2 cut(s) 27, 817
Hpy188III TCNNGA 9 cut(s) 147, 230, 365, 548, 645, 866, 980, 1037, 1102
Hpy8I GTNNAC 3 cut(s) 174, 326, 443
Hpy99I CGWCG 1 cut(s) 531
HpyAV CCTTC 3 cut(s) 30, 383, 579
HpyCH4III ACNGT 2 cut(s) 483, 821
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 8 cut(s) 38, 183, 293, 443, 473, 588, 603, 937
HpyF10VI GCNNNNNNNGC 1 cut(s) 280
HpyF3I CTNAG 8 cut(s) 24, 128, 154, 276, 814, 893, 1095, 1100
HpySE526I ACGT 1 cut(s) 453
Hsp92II CATG 4 cut(s) 114, 187, 368, 1124
HspAI GCGC 1 cut(s) 890
Kpn2I TCCGGA 1 cut(s) 547
KpnI GGTACC 1 cut(s) 853
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 5 cut(s) 68, 241, 361, 544, 565
LguI GCTCTTC 2 cut(s) 800, 1056
LmnI GCTCC 4 cut(s) 280, 724, 989, 1081
LweI GCATC 2 cut(s) 482, 1014
MabI ACCWGGT 1 cut(s) 436
MaeI CTAG 5 cut(s) 48, 134, 147, 230, 513
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 3 cut(s) 186, 220, 590
MalI GATC 5 cut(s) 70, 243, 363, 546, 567
MboI GATC 5 cut(s) 68, 241, 361, 544, 565
MflI RGATCY 2 cut(s) 241, 565
MhlI GDGCHC 2 cut(s) 445, 994
MluCI AATT 5 cut(s) 51, 315, 766, 839, 871
MlyI GAGTC 3 cut(s) 247, 414, 1062
MmeI TCCRAC 1 cut(s) 68
MnlI CCTC 8 cut(s) 129, 132, 237, 423, 456, 722, 914, 1061
Mph1103I ATGCAT 1 cut(s) 185
MroI TCCGGA 1 cut(s) 547
MseI TTAA 5 cut(s) 78, 260, 627, 1014, 1047
MspCI CTTAAG 1 cut(s) 1046
MspI CCGG 6 cut(s) 548, 615, 636, 711, 846, 1029
MspR9I CCNGG 7 cut(s) 336, 417, 438, 615, 847, 1029, 1030
MvaI CCWGG 3 cut(s) 336, 417, 438
MwoI GCNNNNNNNGC 1 cut(s) 280
NciI CCSGG 4 cut(s) 615, 847, 1029, 1030
NdeII GATC 5 cut(s) 68, 241, 361, 544, 565
NlaIII CATG 4 cut(s) 114, 187, 368, 1124
NlaIV GGNNCC 4 cut(s) 117, 282, 709, 851
NmuCI GTSAC 1 cut(s) 186
NsiI ATGCAT 1 cut(s) 185
NspI RCATGY 2 cut(s) 187, 1124
NspV TTCGAA 1 cut(s) 140
PagI TCATGA 1 cut(s) 364
PceI AGGCCT 1 cut(s) 274
PciSI GCTCTTC 2 cut(s) 800, 1056
PfeI GAWTC 4 cut(s) 137, 424, 491, 1033
PinAI ACCGGT 1 cut(s) 635
PleI GAGTC 3 cut(s) 247, 413, 1061
PpsI GAGTC 3 cut(s) 247, 413, 1061
PpuMI RGGWCCY 1 cut(s) 116
Psp124BI GAGCTC 1 cut(s) 994
Psp1406I AACGTT 1 cut(s) 453
Psp5II RGGWCCY 1 cut(s) 116
Psp6I CCWGG 3 cut(s) 334, 415, 436
PspFI CCCAGC 1 cut(s) 1077
PspGI CCWGG 3 cut(s) 334, 415, 436
PspN4I GGNNCC 4 cut(s) 117, 282, 709, 851
PspPI GGNCC 4 cut(s) 116, 171, 323, 638
PspPPI RGGWCCY 1 cut(s) 116
PsuI RGATCY 2 cut(s) 241, 565
RsaI GTAC 3 cut(s) 725, 851, 881
RsaNI GTAC 3 cut(s) 724, 850, 880
SacI GAGCTC 1 cut(s) 994
SapI GCTCTTC 2 cut(s) 800, 1056
SaqAI TTAA 5 cut(s) 78, 260, 627, 1014, 1047
Sau3AI GATC 5 cut(s) 68, 241, 361, 544, 565
Sau96I GGNCC 4 cut(s) 116, 171, 323, 638
SchI GAGTC 3 cut(s) 247, 414, 1062
ScrFI CCNGG 7 cut(s) 336, 417, 438, 615, 847, 1029, 1030
SduI GDGCHC 2 cut(s) 445, 994
SexAI ACCWGGT 1 cut(s) 436
SfaNI GCATC 2 cut(s) 482, 1014
SfuI TTCGAA 1 cut(s) 140
SinI GGWCC 4 cut(s) 116, 171, 323, 638
SmaI CCCGGG 1 cut(s) 1030
SmlI CTYRAG 1 cut(s) 1046
SmoI CTYRAG 1 cut(s) 1046
Sse9I AATT 5 cut(s) 51, 315, 766, 839, 871
SseBI AGGCCT 1 cut(s) 274
SsiI CCGC 2 cut(s) 853, 1008
SspMI CTAG 5 cut(s) 48, 134, 147, 230, 513
SstI GAGCTC 1 cut(s) 994
StuI AGGCCT 1 cut(s) 274
StyD4I CCNGG 7 cut(s) 334, 415, 436, 613, 845, 1027, 1028
StyI CCWWGG 1 cut(s) 799
TaaI ACNGT 2 cut(s) 483, 821
TaiI ACGT 1 cut(s) 456
TaqI TCGA 4 cut(s) 62, 140, 467, 529
TasI AATT 5 cut(s) 51, 315, 766, 839, 871
TfiI GAWTC 4 cut(s) 137, 424, 491, 1033
Tru1I TTAA 5 cut(s) 78, 260, 627, 1014, 1047
Tru9I TTAA 5 cut(s) 78, 260, 627, 1014, 1047
TscAI CASTG 2 cut(s) 106, 1121
TseFI GTSAC 1 cut(s) 186
Tsp45I GTSAC 1 cut(s) 186
TspDTI ATGAA 2 cut(s) 849, 978
TspGWI ACGGA 4 cut(s) 22, 515, 711, 795
TspMI CCCGGG 1 cut(s) 1028
TspRI CASTG 2 cut(s) 106, 1121
Vha464I CTTAAG 1 cut(s) 1046
VneI GTGCAC 1 cut(s) 441
VpaK11BI GGWCC 4 cut(s) 116, 171, 323, 638
XapI RAATTY 2 cut(s) 839, 871
XbaI TCTAGA 2 cut(s) 146, 229
XceI RCATGY 2 cut(s) 187, 1124
XmaI CCCGGG 1 cut(s) 1028
XspI CTAG 5 cut(s) 48, 134, 147, 230, 513
Zsp2I ATGCAT 1 cut(s) 185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.