pycom05g25490

Ferredoxin-NADP reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006772
Physical Location & Seq
Reverse (-)
26852613 .. 26855044
2432 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g25490.4

Sequence Viewer

Length: 1023 bp
ATGTCTCATTGTGCTCTCTCTCAGGGTTCACTGACTGTTCCTGTTGCAAGTGATTTCTCCCTCAGAAGATCTGTGTTCAAGGCACGAAGTGTTAACTTTAATGATAAATCATGGGCACCTATTTTACCTTTGGACTTGAAGACGAAGAATGGGCAGCTGAGAAATCAGCGCATTGTATGCATGTCGGTGCAACAAGCTAGTGCACCTAAAGTTAATGTAGCTCCTTTACAACTTGAGGATGCTAATGAACCCCCATTAAATATATACAAGCCTAAGGAACCTTATACTGCAACCATTCTTTCTGTTGAGAGGATTGTCGGCCCAAAGGCTCCTGGTGAGACTTGCCATATTGTTATTGATCATGGTGGTAATGTGCCCTACTGGGAAGGGCAAAGTTATGGTGTAATTCCTCCTGGTAAAAACCCAAAGAAACCTGGGGCTCCACACAATGTTCGTCTATACTCAATTGCATCCTCAAGGTATGGAGACTATTTTGATGGCAAGACAACCAGTTTGTGTGTCCGCCGTGCTGTCTACTATGACCCTGAGACAGGAAAGGAGGATCGATCCAAGAATGGTGTATGCAGCAACTTTTTATGCAACTCCAAACCTGGTGATAAAATTAAGATCACAGGGCCTGCTGGCAAGATCATGCTTTTGCCTGAAGACGACCCAAATGCCACCCACATTATGATCGCCACTGGCACTGGTGTTGCCCCATACAGAGGTTACCTTCGTCGCATGTTCATGGAATCTGTTCCCAAATTCAAGTTTGGCGGCCTTGCATGGCTATTCCTCGGTGTGGCCAACACTGATAGTCTCCTCTATAATGAAGAATTCACCAAGTATCTCAAAGACTACCCTGATAATTTCCGGTATGACATAGCACTTAGCAGAGAACAGAAGAACCAGAGAGGAGGGAAGATGTATGTTCAGGATAAAATTGAGGAGTATAGTGACGATCGATCTTTAAGCTACTGGATAATGGGGCCCACATATACTTCTGCGGGCTCAAGGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

341

Amino Acids

37.93

Weight (kDa)

9.15

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 115
AccI GTMKAC 1 cut(s) 534
AciI CCGC 3 cut(s) 523, 777, 1007
AclWI GGATC 2 cut(s) 561, 570
AcoI YGGCCR 1 cut(s) 804
AcsI RAATTY 2 cut(s) 764, 836
AcuI CTGAAG 1 cut(s) 684
AdeI CACNNNGTG 1 cut(s) 89
AfiI CCNNNNNNNGG 3 cut(s) 551, 725, 802
AgsI TTSAA 3 cut(s) 79, 139, 769
AjnI CCWGG 4 cut(s) 331, 412, 433, 610
AluBI AGCT 4 cut(s) 157, 197, 221, 975
AluI AGCT 4 cut(s) 157, 197, 221, 975
Alw21I GWGCWC 2 cut(s) 16, 205
Alw26I GTCTC 5 cut(s) 9, 332, 480, 542, 824
Alw44I GTGCAC 1 cut(s) 201
AlwI GGATC 2 cut(s) 561, 570
AlwNI CAGNNNCTG 1 cut(s) 638
AoxI GGCC 5 cut(s) 319, 635, 778, 804, 989
ApaI GGGCCC 1 cut(s) 993
ApaLI GTGCAC 1 cut(s) 201
ApeKI GCWGC 2 cut(s) 154, 585
ApoI RAATTY 2 cut(s) 764, 836
ArsI GACNNNNNNTTYG 2 cut(s) 496, 528
Asp700I GAANNNNTTC 1 cut(s) 756
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 4 cut(s) 320, 635, 989, 990
AsuHPI GGTGA 3 cut(s) 347, 626, 832
AxyI CCTNAGG 1 cut(s) 273
BaeGI GKGCMC 4 cut(s) 118, 205, 378, 993
BalI TGGCCA 1 cut(s) 806
BanI GGYRCC 1 cut(s) 115
BanII GRGCYC 3 cut(s) 442, 993, 1013
BbsI GAAGAC 2 cut(s) 146, 672
Bbv12I GWGCWC 2 cut(s) 16, 205
BbvI GCAGC 2 cut(s) 166, 597
BccI CCATC 1 cut(s) 491
BceAI ACGGC 1 cut(s) 510
BcgI CGANNNNNNTGC 2 cut(s) 659, 693
BciT130I CCWGG 4 cut(s) 333, 414, 435, 612
BclI TGATCA 1 cut(s) 358
BcoDI GTCTC 5 cut(s) 9, 332, 480, 542, 824
BfaI CTAG 1 cut(s) 198
BglII AGATCT 1 cut(s) 68
BisI GCNGC 3 cut(s) 155, 586, 778
BlsI GCNGC 3 cut(s) 156, 587, 779
Bme1390I CCNGG 4 cut(s) 333, 414, 435, 612
BmgT120I GGNCC 4 cut(s) 320, 635, 989, 990
BmiI GGNNCC 6 cut(s) 117, 279, 330, 441, 990, 991
BmrFI CCNGG 4 cut(s) 333, 414, 435, 612
BmrI ACTGGG 1 cut(s) 391
BmsI GCATC 2 cut(s) 229, 479
BmuI ACTGGG 1 cut(s) 391
BpiI GAAGAC 2 cut(s) 146, 672
BpuEI CTTGAG 3 cut(s) 254, 460, 997
Bsa29I ATCGAT 2 cut(s) 565, 964
BsaJI CCNNGG 2 cut(s) 434, 796
BsaWI WCCGGW 1 cut(s) 873
Bsc4I CCNNNNNNNGG 3 cut(s) 551, 725, 802
Bse1I ACTGG 5 cut(s) 386, 510, 706, 712, 983
Bse21I CCTNAGG 1 cut(s) 273
BseBI CCWGG 4 cut(s) 333, 414, 435, 612
BseCI ATCGAT 2 cut(s) 565, 964
BseDI CCNNGG 2 cut(s) 434, 796
BseGI GGATG 2 cut(s) 244, 470
BseLI CCNNNNNNNGG 3 cut(s) 551, 725, 802
BseMII CTCAG 4 cut(s) 35, 76, 149, 537
BseNI ACTGG 5 cut(s) 386, 510, 706, 712, 983
BseRI GAGGAG 3 cut(s) 812, 930, 962
BseSI GKGCMC 4 cut(s) 118, 205, 378, 993
BseXI GCAGC 2 cut(s) 166, 597
Bsh1285I CGRYCG 1 cut(s) 964
BshFI GGCC 5 cut(s) 321, 637, 780, 806, 991
BshNI GGYRCC 1 cut(s) 115
BshVI ATCGAT 2 cut(s) 565, 964
BsiEI CGRYCG 1 cut(s) 964
BsiHKAI GWGCWC 2 cut(s) 16, 205
BsiSI CCGG 1 cut(s) 874
BslI CCNNNNNNNGG 3 cut(s) 551, 725, 802
BsmAI GTCTC 5 cut(s) 9, 332, 480, 542, 824
BsnI GGCC 5 cut(s) 321, 637, 780, 806, 991
Bsp120I GGGCCC 1 cut(s) 989
Bsp1286I GDGCHC 7 cut(s) 16, 118, 205, 378, 442, 993, 1013
Bsp143I GATC 9 cut(s) 68, 358, 562, 566, 627, 648, 693, 961, 965
BspACI CCGC 3 cut(s) 523, 777, 1007
BspANI GGCC 5 cut(s) 321, 637, 780, 806, 991
BspCNI CTCAG 4 cut(s) 34, 75, 150, 538
BspDI ATCGAT 2 cut(s) 565, 964
BspLI GGNNCC 6 cut(s) 117, 279, 330, 441, 990, 991
BspPI GGATC 2 cut(s) 561, 570
BspT107I GGYRCC 1 cut(s) 115
BsrI ACTGG 5 cut(s) 386, 510, 706, 712, 983
BssECI CCNNGG 2 cut(s) 434, 796
BssMI GATC 9 cut(s) 68, 358, 562, 566, 627, 648, 693, 961, 965
Bst2UI CCWGG 4 cut(s) 333, 414, 435, 612
Bst4CI ACNGT 1 cut(s) 37
BstAPI GCANNNNNTGC 1 cut(s) 177
BstC8I GCNNGC 3 cut(s) 639, 643, 1009
BstDEI CTNAG 6 cut(s) 21, 62, 158, 273, 546, 890
BstEII GGTNACC 1 cut(s) 728
BstENI CCTNNNNNAGG 1 cut(s) 549
BstF5I GGATG 2 cut(s) 244, 470
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 9 cut(s) 71, 361, 565, 569, 630, 651, 696, 964, 968
BstMAI GTCTC 5 cut(s) 9, 332, 480, 542, 824
BstMBI GATC 9 cut(s) 68, 358, 562, 566, 627, 648, 693, 961, 965
BstMCI CGRYCG 1 cut(s) 964
BstMWI GCNNNNNNNGC 1 cut(s) 177
BstNI CCWGG 4 cut(s) 333, 414, 435, 612
BstNSI RCATGY 2 cut(s) 184, 745
BstPI GGTNACC 1 cut(s) 728
BstSCI CCNGG 4 cut(s) 331, 412, 433, 610
BstSLI GKGCMC 4 cut(s) 118, 205, 378, 993
BstV1I GCAGC 2 cut(s) 166, 597
BstV2I GAAGAC 2 cut(s) 146, 672
BstX2I RGATCY 1 cut(s) 68
BstYI RGATCY 1 cut(s) 68
Bsu15I ATCGAT 2 cut(s) 565, 964
Bsu36I CCTNAGG 1 cut(s) 273
BsuRI GGCC 5 cut(s) 321, 637, 780, 806, 991
BsuTUI ATCGAT 2 cut(s) 565, 964
BtsCI GGATG 2 cut(s) 244, 470
BtsIMutI CAGTG 4 cut(s) 29, 699, 705, 810
Cac8I GCNNGC 3 cut(s) 639, 643, 1009
CaiI CAGNNNCTG 1 cut(s) 638
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 4 cut(s) 320, 635, 989, 990
ClaI ATCGAT 2 cut(s) 565, 964
CsiI ACCWGGT 1 cut(s) 610
CviAII CATG 7 cut(s) 111, 181, 362, 652, 742, 748, 786
DdeI CTNAG 6 cut(s) 21, 62, 158, 273, 546, 890
DpnI GATC 9 cut(s) 70, 360, 564, 568, 629, 650, 695, 963, 967
DpnII GATC 9 cut(s) 68, 358, 562, 566, 627, 648, 693, 961, 965
DraIII CACNNNGTG 1 cut(s) 89
EaeI YGGCCR 1 cut(s) 804
EciI GGCGGA 1 cut(s) 512
Eco24I GRGCYC 3 cut(s) 442, 993, 1013
Eco57I CTGAAG 1 cut(s) 684
Eco81I CCTNAGG 1 cut(s) 273
Eco91I GGTNACC 1 cut(s) 728
EcoNI CCTNNNNNAGG 1 cut(s) 549
EcoO109I RGGNCCY 2 cut(s) 635, 989
EcoO65I GGTNACC 1 cut(s) 728
EcoRI GAATTC 1 cut(s) 836
EcoRII CCWGG 4 cut(s) 331, 412, 433, 610
EcoT22I ATGCAT 1 cut(s) 182
EcoT38I GRGCYC 3 cut(s) 442, 993, 1013
FaeI CATG 7 cut(s) 114, 184, 365, 655, 745, 751, 789
FatI CATG 7 cut(s) 110, 180, 361, 651, 741, 747, 785
FauI CCCGC 1 cut(s) 1000
FbaI TGATCA 1 cut(s) 358
FblI GTMKAC 1 cut(s) 534
Fnu4HI GCNGC 3 cut(s) 155, 586, 778
FokI GGATG 2 cut(s) 251, 457
FriOI GRGCYC 3 cut(s) 442, 993, 1013
Fsp4HI GCNGC 3 cut(s) 155, 586, 778
FspBI CTAG 1 cut(s) 198
GlaI GCGC 1 cut(s) 170
GluI GCNGC 3 cut(s) 155, 586, 778
HaeIII GGCC 5 cut(s) 321, 637, 780, 806, 991
HapII CCGG 1 cut(s) 874
HhaI GCGC 1 cut(s) 171
Hin1II CATG 7 cut(s) 114, 184, 365, 655, 745, 751, 789
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 1 cut(s) 752
HpaI GTTAAC 1 cut(s) 94
HpaII CCGG 1 cut(s) 874
HphI GGTGA 3 cut(s) 347, 626, 832
Hpy166II GTNNAC 4 cut(s) 29, 94, 203, 535
Hpy188I TCNGA 1 cut(s) 65
Hpy188III TCNNGA 1 cut(s) 935
Hpy8I GTNNAC 4 cut(s) 29, 94, 203, 535
Hpy99I CGWCG 1 cut(s) 741
HpyAV CCTTC 2 cut(s) 380, 743
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4V TGCA 9 cut(s) 47, 180, 190, 203, 290, 470, 585, 600, 785
HpyF10VI GCNNNNNNNGC 1 cut(s) 177
HpyF3I CTNAG 6 cut(s) 21, 62, 158, 273, 546, 890
Hsp92II CATG 7 cut(s) 114, 184, 365, 655, 745, 751, 789
HspAI GCGC 1 cut(s) 169
Ksp22I TGATCA 1 cut(s) 358
KspAI GTTAAC 1 cut(s) 94
Kzo9I GATC 9 cut(s) 68, 358, 562, 566, 627, 648, 693, 961, 965
LmnI GCTCC 3 cut(s) 226, 334, 445
Lsp1109I GCAGC 2 cut(s) 166, 597
LweI GCATC 2 cut(s) 229, 479
MabI ACCWGGT 1 cut(s) 610
MaeI CTAG 1 cut(s) 198
MaeIII GTNAC 2 cut(s) 728, 956
MalI GATC 9 cut(s) 70, 360, 564, 568, 629, 650, 695, 963, 967
MboI GATC 9 cut(s) 68, 358, 562, 566, 627, 648, 693, 961, 965
MboII GAAGA 7 cut(s) 78, 151, 157, 677, 845, 916, 934
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 1 cut(s) 68
MhlI GDGCHC 7 cut(s) 16, 118, 205, 378, 442, 993, 1013
MlsI TGGCCA 1 cut(s) 806
MluCI AATT 7 cut(s) 405, 465, 621, 764, 836, 868, 942
MluNI TGGCCA 1 cut(s) 806
Mox20I TGGCCA 1 cut(s) 806
Mph1103I ATGCAT 1 cut(s) 182
MroXI GAANNNNTTC 1 cut(s) 756
MscI TGGCCA 1 cut(s) 806
MseI TTAA 6 cut(s) 93, 99, 213, 257, 624, 971
MslI CAYNNNNRTG 2 cut(s) 185, 746
Msp20I TGGCCA 1 cut(s) 806
MspA1I CMGCKG 1 cut(s) 157
MspI CCGG 1 cut(s) 874
MspR9I CCNGG 4 cut(s) 333, 414, 435, 612
MunI CAATTG 1 cut(s) 465
MvaI CCWGG 4 cut(s) 333, 414, 435, 612
MwoI GCNNNNNNNGC 1 cut(s) 177
NdeII GATC 9 cut(s) 68, 358, 562, 566, 627, 648, 693, 961, 965
NlaIII CATG 7 cut(s) 114, 184, 365, 655, 745, 751, 789
NlaIV GGNNCC 6 cut(s) 117, 279, 330, 441, 990, 991
NmuCI GTSAC 1 cut(s) 956
NsiI ATGCAT 1 cut(s) 182
NspI RCATGY 2 cut(s) 184, 745
PdmI GAANNNNTTC 1 cut(s) 756
PfeI GAWTC 1 cut(s) 752
PkrI GCNGC 3 cut(s) 156, 587, 779
Ple19I CGATCG 1 cut(s) 964
Psp6I CCWGG 4 cut(s) 331, 412, 433, 610
PspEI GGTNACC 1 cut(s) 728
PspGI CCWGG 4 cut(s) 331, 412, 433, 610
PspN4I GGNNCC 6 cut(s) 117, 279, 330, 441, 990, 991
PspOMI GGGCCC 1 cut(s) 989
PspPI GGNCC 4 cut(s) 320, 635, 989, 990
PstNI CAGNNNCTG 1 cut(s) 638
PsuI RGATCY 1 cut(s) 68
PvuI CGATCG 1 cut(s) 964
PvuII CAGCTG 1 cut(s) 157
RseI CAYNNNNRTG 2 cut(s) 185, 746
SaqAI TTAA 6 cut(s) 93, 99, 213, 257, 624, 971
SatI GCNGC 3 cut(s) 155, 586, 778
Sau3AI GATC 9 cut(s) 68, 358, 562, 566, 627, 648, 693, 961, 965
Sau96I GGNCC 4 cut(s) 320, 635, 989, 990
ScrFI CCNGG 4 cut(s) 333, 414, 435, 612
SduI GDGCHC 7 cut(s) 16, 118, 205, 378, 442, 993, 1013
SexAI ACCWGGT 1 cut(s) 610
SfaNI GCATC 2 cut(s) 229, 479
SmiMI CAYNNNNRTG 2 cut(s) 185, 746
SmlI CTYRAG 3 cut(s) 233, 475, 1012
SmoI CTYRAG 3 cut(s) 233, 475, 1012
Sse9I AATT 7 cut(s) 405, 465, 621, 764, 836, 868, 942
SsiI CCGC 3 cut(s) 523, 777, 1007
SspMI CTAG 1 cut(s) 198
StyD4I CCNGG 4 cut(s) 331, 412, 433, 610
TaaI ACNGT 1 cut(s) 37
TaqI TCGA 2 cut(s) 565, 964
TasI AATT 7 cut(s) 405, 465, 621, 764, 836, 868, 942
TauI GCSGC 1 cut(s) 780
TfiI GAWTC 1 cut(s) 752
Tru1I TTAA 6 cut(s) 93, 99, 213, 257, 624, 971
Tru9I TTAA 6 cut(s) 93, 99, 213, 257, 624, 971
TscAI CASTG 4 cut(s) 36, 706, 712, 817
TseFI GTSAC 1 cut(s) 956
TseI GCWGC 2 cut(s) 154, 585
Tsp45I GTSAC 1 cut(s) 956
TspDTI ATGAA 3 cut(s) 261, 736, 846
TspRI CASTG 4 cut(s) 36, 706, 712, 817
VneI GTGCAC 1 cut(s) 201
XagI CCTNNNNNAGG 1 cut(s) 549
XapI RAATTY 2 cut(s) 764, 836
XceI RCATGY 2 cut(s) 184, 745
XmiI GTMKAC 1 cut(s) 534
XmnI GAANNNNTTC 1 cut(s) 756
XspI CTAG 1 cut(s) 198
Zsp2I ATGCAT 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.