Rw5G010720

Ferredoxin-NADP reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0006772
Physical Location & Seq
Forward (+)
11756026 .. 11759174
3149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G010720.1

Sequence Viewer

Length: 1140 bp
ATGTCTCACTTGGCTCTCTCTCAGGTTTCACTTACTACTACTGTTCCTGCTGGAAGTGACTTATCTCTCAGAAGATCTGTGTTCAAGGCACGAAGTGTAAATTTTAGTGATAAATCATGGGCACCAGTTTCAACTTTGGACTTGAAAACAAAGAGTGGGCGGCTACGAAACAAGCGCATAGTATGCATGTCAGTGCAACAAGCAAGTGCACCAAAAGTTGCTGTGTCTCCTTTAGAACTCGAAAATGCTACAGAACCCCCATTGAATATATACAAGCCGAAGGAACCTTATACCGCAACCATTTTATCTGTTGAGAGGATTGTCGGCCCAAGGGCTCCTGGAGAAACTTGCCATATAGTGATTGATCATGGTGGTAATGTTCCCTATTGGGAAGGACAAAGTTATGGCGTTATCCCTCCTGGTGAAAACCCAAAGAAACCTGGTGCTCCACACAATGTCCGTCTTTACTCAATTGCATCGACAAGGTATGGAGACTATTTCGATGGCAAGACAACTAGTTTGTGTGTCCGCCGTGCTGTCTATTATGACCCAGAGACAGGGAAGGAGGATCCCTCCAAAAATGGTGTATGCAGCAACTATTTATGCAACTCTAAACCCGGAGATAAAATTAAGATCACAGGTCCTGCTGGCAAGATCATGCTCCTGCCTGAAGACAACCCCAATGCCACCCATATTATGATTGCCACTGGCACTGGTGTTGCTCCATACAGAGGTTACCTTCGTCGTATGTTCATGGAAGCTGTTCCTACATTCAAGTTTGGTGGCCTTGCATGGTTGTTCCTTGGAGTGGCCAATACCGACAGCCTCCTCTATGATGAAGAATTCCGCAAGTATCTTAAGGACTACCCCGATAATTTCCGATACGACATAGCACTTAGTAGAGAACAAAAGAACAAGAGAGGAGGAAAGATGTATGTTCAGGATAAAATTGAAGAATACAGTGATGAGATCTTTAAACTCTTGGATGGTGGGGCCCATATCTACTTCTGTGGGCTCAAGGGTATGATGCCTGGAATTCAAGAAACCTTGAAGAGGGTTGCAGAAGAAAGAGGGGAAAGTTGGGAGGAGAAGCTTTCACAACTGAAGAAGAATAAGCAATGGCATGTTGAAGTTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

379

Amino Acids

42.45

Weight (kDa)

9.03

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 233 - 347 6.5e-25 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 121
AciI CCGC 4 cut(s) 160, 294, 529, 847
AclWI GGATC 2 cut(s) 563, 576
AcoI YGGCCR 1 cut(s) 810
AcsI RAATTY 3 cut(s) 100, 842, 1035
AcuI CTGAAG 2 cut(s) 690, 1124
AdeI CACNNNGTG 1 cut(s) 95
AfiI CCNNNNNNNGG 4 cut(s) 557, 731, 808, 1053
AflII CTTAAG 1 cut(s) 857
AgsI TTSAA 9 cut(s) 85, 132, 145, 265, 775, 953, 1040, 1051, 1130
AhlI ACTAGT 1 cut(s) 515
AjnI CCWGG 4 cut(s) 337, 418, 439, 1030
AluBI AGCT 2 cut(s) 761, 1093
AluI AGCT 2 cut(s) 761, 1093
Alw21I GWGCWC 2 cut(s) 211, 448
Alw26I GTCTC 4 cut(s) 9, 231, 486, 548
Alw44I GTGCAC 1 cut(s) 207
AlwI GGATC 2 cut(s) 563, 576
AlwNI CAGNNNCTG 1 cut(s) 644
AoxI GGCC 4 cut(s) 325, 784, 810, 993
ApaI GGGCCC 1 cut(s) 997
ApaLI GTGCAC 1 cut(s) 207
ApeKI GCWGC 1 cut(s) 591
ApoI RAATTY 3 cut(s) 100, 842, 1035
ArsI GACNNNNNNTTYG 2 cut(s) 502, 534
Asp700I GAANNNNTTC 1 cut(s) 762
AspLEI GCGC 1 cut(s) 177
AspS9I GGNCC 4 cut(s) 326, 641, 993, 994
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 1 cut(s) 434
AvaII GGWCC 1 cut(s) 641
BaeGI GKGCMC 3 cut(s) 124, 211, 997
BalI TGGCCA 1 cut(s) 812
BamHI GGATCC 1 cut(s) 568
BanI GGYRCC 1 cut(s) 121
BanII GRGCYC 3 cut(s) 337, 997, 1017
BbsI GAAGAC 1 cut(s) 678
Bbv12I GWGCWC 2 cut(s) 211, 448
BbvI GCAGC 1 cut(s) 603
BccI CCATC 2 cut(s) 497, 980
BceAI ACGGC 1 cut(s) 516
BciT130I CCWGG 4 cut(s) 339, 420, 441, 1032
BclI TGATCA 1 cut(s) 364
BcnI CCSGG 1 cut(s) 618
BcoDI GTCTC 4 cut(s) 9, 231, 486, 548
BcuI ACTAGT 1 cut(s) 515
BfaI CTAG 1 cut(s) 516
BfmI CTRYAG 1 cut(s) 249
BfrI CTTAAG 1 cut(s) 857
BglII AGATCT 2 cut(s) 74, 969
BisI GCNGC 2 cut(s) 161, 592
BlsI GCNGC 2 cut(s) 162, 593
Bme1390I CCNGG 5 cut(s) 339, 420, 441, 618, 1032
Bme18I GGWCC 1 cut(s) 641
BmgT120I GGNCC 4 cut(s) 326, 641, 993, 994
BmiI GGNNCC 6 cut(s) 123, 285, 336, 570, 994, 995
BmrFI CCNGG 5 cut(s) 339, 420, 441, 618, 1032
BmsI GCATC 2 cut(s) 485, 1017
BpiI GAAGAC 1 cut(s) 678
BplI GAGNNNNNCTC 2 cut(s) 557, 589
BpmI CTGGAG 1 cut(s) 360
BpuEI CTTGAG 1 cut(s) 1001
BpuMI CCSGG 1 cut(s) 618
BsaJI CCNNGG 2 cut(s) 329, 802
Bsc4I CCNNNNNNNGG 4 cut(s) 557, 731, 808, 1053
Bse1I ACTGG 3 cut(s) 125, 712, 718
Bse3DI GCAATG 1 cut(s) 1124
BseBI CCWGG 4 cut(s) 339, 420, 441, 1032
BseDI CCNNGG 2 cut(s) 329, 802
BseGI GGATG 1 cut(s) 991
BseLI CCNNNNNNNGG 4 cut(s) 557, 731, 808, 1053
BseMI GCAATG 1 cut(s) 1124
BseMII CTCAG 2 cut(s) 35, 82
BseNI ACTGG 3 cut(s) 125, 712, 718
BseRI GAGGAG 3 cut(s) 818, 936, 1100
BseSI GKGCMC 3 cut(s) 124, 211, 997
BseXI GCAGC 1 cut(s) 603
BshFI GGCC 4 cut(s) 327, 786, 812, 995
BshNI GGYRCC 1 cut(s) 121
BsiHKAI GWGCWC 2 cut(s) 211, 448
BsiSI CCGG 1 cut(s) 618
BslI CCNNNNNNNGG 4 cut(s) 557, 731, 808, 1053
BsmAI GTCTC 4 cut(s) 9, 231, 486, 548
BsnI GGCC 4 cut(s) 327, 786, 812, 995
Bsp120I GGGCCC 1 cut(s) 993
Bsp1286I GDGCHC 6 cut(s) 124, 211, 337, 448, 997, 1017
Bsp143I GATC 6 cut(s) 74, 364, 568, 633, 654, 969
BspACI CCGC 4 cut(s) 160, 294, 529, 847
BspANI GGCC 4 cut(s) 327, 786, 812, 995
BspCNI CTCAG 2 cut(s) 34, 81
BspLI GGNNCC 6 cut(s) 123, 285, 336, 570, 994, 995
BspPI GGATC 2 cut(s) 563, 576
BspT107I GGYRCC 1 cut(s) 121
BspTI CTTAAG 1 cut(s) 857
BsrDI GCAATG 1 cut(s) 1124
BsrI ACTGG 3 cut(s) 125, 712, 718
BssECI CCNNGG 2 cut(s) 329, 802
BssMI GATC 6 cut(s) 74, 364, 568, 633, 654, 969
BssT1I CCWWGG 2 cut(s) 329, 802
Bst2UI CCWGG 4 cut(s) 339, 420, 441, 1032
Bst4CI ACNGT 2 cut(s) 43, 962
Bst6I CTCTTC 1 cut(s) 1046
BstAFI CTTAAG 1 cut(s) 857
BstAPI GCANNNNNTGC 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 649
BstDEI CTNAG 3 cut(s) 21, 68, 896
BstEII GGTNACC 1 cut(s) 734
BstENI CCTNNNNNAGG 1 cut(s) 1051
BstF5I GGATG 1 cut(s) 991
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 6 cut(s) 77, 367, 571, 636, 657, 972
BstMAI GTCTC 4 cut(s) 9, 231, 486, 548
BstMBI GATC 6 cut(s) 74, 364, 568, 633, 654, 969
BstMWI GCNNNNNNNGC 1 cut(s) 183
BstNI CCWGG 4 cut(s) 339, 420, 441, 1032
BstNSI RCATGY 2 cut(s) 190, 1127
BstPI GGTNACC 1 cut(s) 734
BstSCI CCNGG 5 cut(s) 337, 418, 439, 616, 1030
BstSFI CTRYAG 1 cut(s) 249
BstSLI GKGCMC 3 cut(s) 124, 211, 997
BstV1I GCAGC 1 cut(s) 603
BstV2I GAAGAC 1 cut(s) 678
BstX2I RGATCY 3 cut(s) 74, 568, 969
BstYI RGATCY 3 cut(s) 74, 568, 969
BsuRI GGCC 4 cut(s) 327, 786, 812, 995
BtsCI GGATG 1 cut(s) 991
BtsIMutI CAGTG 4 cut(s) 198, 705, 711, 967
Cac8I GCNNGC 1 cut(s) 649
CaiI CAGNNNCTG 1 cut(s) 644
CfoI GCGC 1 cut(s) 177
Cfr13I GGNCC 4 cut(s) 326, 641, 993, 994
CsiI ACCWGGT 1 cut(s) 439
CspCI CAANNNNNGTGG 2 cut(s) 763, 798
CviAII CATG 7 cut(s) 117, 187, 368, 658, 754, 792, 1124
DdeI CTNAG 3 cut(s) 21, 68, 896
DpnI GATC 6 cut(s) 76, 366, 570, 635, 656, 971
DpnII GATC 6 cut(s) 74, 364, 568, 633, 654, 969
DraI TTTAAA 1 cut(s) 976
DraIII CACNNNGTG 1 cut(s) 95
EaeI YGGCCR 1 cut(s) 810
Eam1104I CTCTTC 1 cut(s) 1046
EarI CTCTTC 1 cut(s) 1046
EciI GGCGGA 1 cut(s) 518
Eco130I CCWWGG 2 cut(s) 329, 802
Eco24I GRGCYC 3 cut(s) 337, 997, 1017
Eco47I GGWCC 1 cut(s) 641
Eco57I CTGAAG 2 cut(s) 690, 1124
Eco91I GGTNACC 1 cut(s) 734
EcoNI CCTNNNNNAGG 1 cut(s) 1051
EcoO109I RGGNCCY 2 cut(s) 641, 993
EcoO65I GGTNACC 1 cut(s) 734
EcoRI GAATTC 2 cut(s) 842, 1035
EcoRII CCWGG 4 cut(s) 337, 418, 439, 1030
EcoT14I CCWWGG 2 cut(s) 329, 802
EcoT22I ATGCAT 1 cut(s) 188
EcoT38I GRGCYC 3 cut(s) 337, 997, 1017
ErhI CCWWGG 2 cut(s) 329, 802
FaeI CATG 7 cut(s) 120, 190, 371, 661, 757, 795, 1127
FatI CATG 7 cut(s) 116, 186, 367, 657, 753, 791, 1123
FbaI TGATCA 1 cut(s) 364
Fnu4HI GCNGC 2 cut(s) 161, 592
FokI GGATG 1 cut(s) 998
FriOI GRGCYC 3 cut(s) 337, 997, 1017
Fsp4HI GCNGC 2 cut(s) 161, 592
FspBI CTAG 1 cut(s) 516
GlaI GCGC 1 cut(s) 176
GluI GCNGC 2 cut(s) 161, 592
GsuI CTGGAG 1 cut(s) 360
HaeIII GGCC 4 cut(s) 327, 786, 812, 995
HapII CCGG 1 cut(s) 618
HhaI GCGC 1 cut(s) 177
Hin1II CATG 7 cut(s) 120, 190, 371, 661, 757, 795, 1127
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HindIII AAGCTT 1 cut(s) 1091
HpaII CCGG 1 cut(s) 618
HphI GGTGA 1 cut(s) 434
Hpy166II GTNNAC 2 cut(s) 209, 1135
Hpy188I TCNGA 2 cut(s) 71, 881
Hpy188III TCNNGA 2 cut(s) 941, 1040
Hpy8I GTNNAC 2 cut(s) 209, 1135
Hpy99I CGWCG 1 cut(s) 747
HpyAV CCTTC 4 cut(s) 274, 386, 556, 749
HpyCH4III ACNGT 2 cut(s) 43, 962
HpyCH4V TGCA 8 cut(s) 186, 196, 209, 476, 591, 606, 791, 1061
HpyF10VI GCNNNNNNNGC 1 cut(s) 183
HpyF3I CTNAG 3 cut(s) 21, 68, 896
Hsp92II CATG 7 cut(s) 120, 190, 371, 661, 757, 795, 1127
HspAI GCGC 1 cut(s) 175
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 6 cut(s) 74, 364, 568, 633, 654, 969
LmnI GCTCC 4 cut(s) 340, 451, 666, 727
Lsp1109I GCAGC 1 cut(s) 603
LweI GCATC 2 cut(s) 485, 1017
MabI ACCWGGT 1 cut(s) 439
MaeI CTAG 1 cut(s) 516
MaeIII GTNAC 2 cut(s) 56, 734
MalI GATC 6 cut(s) 76, 366, 570, 635, 656, 971
MboI GATC 6 cut(s) 74, 364, 568, 633, 654, 969
MboII GAAGA 8 cut(s) 84, 683, 851, 965, 1063, 1076, 1117, 1120
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 3 cut(s) 74, 568, 969
MhlI GDGCHC 6 cut(s) 124, 211, 337, 448, 997, 1017
MlsI TGGCCA 1 cut(s) 812
MluCI AATT 7 cut(s) 100, 471, 627, 842, 874, 948, 1035
MluNI TGGCCA 1 cut(s) 812
Mox20I TGGCCA 1 cut(s) 812
Mph1103I ATGCAT 1 cut(s) 188
MroXI GAANNNNTTC 1 cut(s) 762
MscI TGGCCA 1 cut(s) 812
MseI TTAA 3 cut(s) 630, 858, 975
MslI CAYNNNNRTG 1 cut(s) 191
Msp20I TGGCCA 1 cut(s) 812
MspCI CTTAAG 1 cut(s) 857
MspI CCGG 1 cut(s) 618
MspR9I CCNGG 5 cut(s) 339, 420, 441, 618, 1032
MunI CAATTG 1 cut(s) 471
MvaI CCWGG 4 cut(s) 339, 420, 441, 1032
MwoI GCNNNNNNNGC 1 cut(s) 183
NciI CCSGG 1 cut(s) 618
NdeII GATC 6 cut(s) 74, 364, 568, 633, 654, 969
NlaIII CATG 7 cut(s) 120, 190, 371, 661, 757, 795, 1127
NlaIV GGNNCC 6 cut(s) 123, 285, 336, 570, 994, 995
NmuCI GTSAC 1 cut(s) 56
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 2 cut(s) 190, 1127
PdmI GAANNNNTTC 1 cut(s) 762
PfoI TCCNGGA 1 cut(s) 337
PkrI GCNGC 2 cut(s) 162, 593
PpuMI RGGWCCY 1 cut(s) 641
Psp5II RGGWCCY 1 cut(s) 641
Psp6I CCWGG 4 cut(s) 337, 418, 439, 1030
PspEI GGTNACC 1 cut(s) 734
PspGI CCWGG 4 cut(s) 337, 418, 439, 1030
PspN4I GGNNCC 6 cut(s) 123, 285, 336, 570, 994, 995
PspOMI GGGCCC 1 cut(s) 993
PspPI GGNCC 4 cut(s) 326, 641, 993, 994
PspPPI RGGWCCY 1 cut(s) 641
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 3 cut(s) 74, 568, 969
RseI CAYNNNNRTG 1 cut(s) 191
SaqAI TTAA 3 cut(s) 630, 858, 975
SatI GCNGC 2 cut(s) 161, 592
Sau3AI GATC 6 cut(s) 74, 364, 568, 633, 654, 969
Sau96I GGNCC 4 cut(s) 326, 641, 993, 994
ScrFI CCNGG 5 cut(s) 339, 420, 441, 618, 1032
SduI GDGCHC 6 cut(s) 124, 211, 337, 448, 997, 1017
SexAI ACCWGGT 1 cut(s) 439
SfaNI GCATC 2 cut(s) 485, 1017
SfcI CTRYAG 1 cut(s) 249
SinI GGWCC 1 cut(s) 641
SmiMI CAYNNNNRTG 1 cut(s) 191
SmlI CTYRAG 2 cut(s) 857, 1016
SmoI CTYRAG 2 cut(s) 857, 1016
SpeI ACTAGT 1 cut(s) 515
Sse9I AATT 7 cut(s) 100, 471, 627, 842, 874, 948, 1035
SsiI CCGC 4 cut(s) 160, 294, 529, 847
SspMI CTAG 1 cut(s) 516
StyD4I CCNGG 5 cut(s) 337, 418, 439, 616, 1030
StyI CCWWGG 2 cut(s) 329, 802
TaaI ACNGT 2 cut(s) 43, 962
TaqI TCGA 3 cut(s) 240, 479, 501
TasI AATT 7 cut(s) 100, 471, 627, 842, 874, 948, 1035
TauI GCSGC 1 cut(s) 163
Tru1I TTAA 3 cut(s) 630, 858, 975
Tru9I TTAA 3 cut(s) 630, 858, 975
TscAI CASTG 4 cut(s) 198, 712, 718, 967
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 1 cut(s) 591
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 2 cut(s) 742, 852
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 4 cut(s) 198, 712, 718, 967
Vha464I CTTAAG 1 cut(s) 857
VneI GTGCAC 1 cut(s) 207
VpaK11BI GGWCC 1 cut(s) 641
XagI CCTNNNNNAGG 1 cut(s) 1051
XapI RAATTY 3 cut(s) 100, 842, 1035
XceI RCATGY 2 cut(s) 190, 1127
XmnI GAANNNNTTC 1 cut(s) 762
XspI CTAG 1 cut(s) 516
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.