Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0006772

Physical Location & Seq

Genomic Coordinates Reverse (-)

23643170 .. 23645206

Gene Structure 2037 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g20890.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1137 bp

ATGTCTCATTTGGCTCTCTCTCAGGGTTCACTGACTGTGACTGTTCCGCTTGGAAGTGATTTCTCCCTCCGAAGATCTGTGTTCAAGGCACGAAGTGTTAACTTTAATGATAAATCATGGGCTCCTATACCTTTGGACTTCAAAACGAAAAAGGGACGGCTGAGAAATCAGCGCATTCTATGCATGTCAGTGCAACAAGCAAGTGCGCCTAAAGTTAATGTAGCTCCTCTACAACTTGAGGATGCTAATGAGCCCCCATTAAATACATACAAGCCTAAGGAGCCTTATACTGCAACCATTCTTTCTGTTGAGAGGATTGTCGGCCCAAATGCTCCTGGAGAGACTTGCCATATTGTGATTGATCATGGTGGTAATGTGCCTTACTGGGAAGGGCAAAGTTATGGTGTCATCCCTCCGGGGGAAAACCCAAAGAAACCTGGGGCTCCGCACAATGTTCGCCTTTACTCAATTGCATCGACAAGGTATGGAGACTATTTTGATGGCAAGACAACTAGTTTGTGTGTTCGTCGTGCTGTCTATTACGACCCTGAGACAGGAAAGGAGGATCGATCCAAGAATGGTGTATGCAGCAACTTTTTATGCAACTCCAAACCTGGTGATAAAATTAAGATCACAGGGCCTGCTGGCAAGATCATGCTTTTGCCTGAAGACAACCCAAATGCCACCCACATTATGATTGCAACTGGCACTGGTATTGCCCCGTACAGAGGTTACCTCCGTCGCATGTTCATGGAATCTGTTCCCAAATTCAAGTTTGGCGGCCTTGCATGGCTATTCCTTGGTGTGGCCAACACTGATAGTCTCCTATATGATGAAGAATTCACCAAGTATCTCAAGGACTACCCCGATAATTTCCGGTACGACATAGCACTTAGCAGAGAACAGAAGAACCAGAGAGGAGGGAAGATGTATGTTCAGGATAAAATCGAGGAGTACAGTGACGAGATCTTTAAGCTATTGGACAACGGGGCCCACATATACTTCTGCGGGCTCAAGGGAATGATGCCCGGGATTCAAGAAACCTTGAAGAGGGTTGCGGTTGAAAGAGGGGAGAGCTGGGAGGAGAAGCTTTCACAGCTGAAAAAGAACAAGCAATGGCATGTGGAAGTCTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00195 ko01100 map00195 map01100

Pfam Domains

Protein Families

NAD_binding_1

Protein Analysis

379

Amino Acids

42.58

Weight (kDa)

9.03

Isoelectric Point (pI)

41.22

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006772)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30510 AT1G30510 AT1G30510 AT4G05390 AT4G05390
fragaria_vesca	FvH4_3g10090 FvH4_3g10090
malus_domestica	MD05G1273100.v1.1 MD10G1253200.v1.1
prunus_persica	Prupe.4G090300_v2.0.a1
pyrus_communis	pycom05g25490 pycom10g20890
rosa_chinensis	RchiOBHm_Chr5g0016001
rosa_laevigata	RLG00000032234
rosa_multiflora	Rmu_co8509133.1_g000001 Rmu_sc0000299.1_g000024 Rmu_sc0001878.1_g000003 Rmu_sc0021845.1_g000003
rosa_roxburghii	Rroxscaffold_1G00060640
rosa_rugosa	Rorug05G0030700 Rorug05G0030700
rosa_samantha	Rh5AG124100 Rh5BG123100 Rh5CG134500 Rh5DG122500
rosa_wichuraiana	Rw5G010720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1131
AciI	CCGC	5 cut(s)	47, 446, 780, 1008, 1058
AclWI	GGATC	2 cut(s)	564, 573
AcoI	YGGCCR	1 cut(s)	807
AcsI	RAATTY	2 cut(s)	767, 839
AcuI	CTGAAG	1 cut(s)	687
AdeI	CACNNNGTG	1 cut(s)	95
AfaI	GTAC	3 cut(s)	725, 881, 956
AfiI	CCNNNNNNNGG	5 cut(s)	418, 554, 728, 805, 1050
AgsI	TTSAA	6 cut(s)	85, 142, 772, 1037, 1048, 1064
AhlI	ACTAGT	1 cut(s)	512
AjnI	CCWGG	3 cut(s)	334, 436, 613
AluBI	AGCT	5 cut(s)	224, 976, 1077, 1090, 1099
AluI	AGCT	5 cut(s)	224, 976, 1077, 1090, 1099
Alw26I	GTCTC	5 cut(s)	9, 335, 483, 545, 827
AlwI	GGATC	2 cut(s)	564, 573
AlwNI	CAGNNNCTG	1 cut(s)	641
Ama87I	CYCGRG	1 cut(s)	1028
AoxI	GGCC	5 cut(s)	322, 638, 781, 807, 990
ApaI	GGGCCC	1 cut(s)	994
ApeKI	GCWGC	1 cut(s)	588
ApoI	RAATTY	2 cut(s)	767, 839
ArsI	GACNNNNNNTTYG	2 cut(s)	499, 531
Asp700I	GAANNNNTTC	1 cut(s)	759
AspLEI	GCGC	2 cut(s)	174, 208
AspS9I	GGNCC	4 cut(s)	323, 638, 990, 991
AsuC2I	CCSGG	3 cut(s)	417, 1029, 1030
AsuHPI	GGTGA	2 cut(s)	629, 835
AvaI	CYCGRG	1 cut(s)	1028
AxyI	CCTNAGG	1 cut(s)	276
BaeGI	GKGCMC	1 cut(s)	994
BalI	TGGCCA	1 cut(s)	809
BanII	GRGCYC	5 cut(s)	124, 255, 445, 994, 1014
BbsI	GAAGAC	1 cut(s)	675
BbvI	GCAGC	1 cut(s)	600
BccI	CCATC	1 cut(s)	494
BceAI	ACGGC	1 cut(s)	173
BcgI	CGANNNNNNTGC	2 cut(s)	437, 471
BciT130I	CCWGG	3 cut(s)	336, 438, 615
BclI	TGATCA	1 cut(s)	361
BcnI	CCSGG	3 cut(s)	417, 1029, 1030
BcoDI	GTCTC	5 cut(s)	9, 335, 483, 545, 827
BcuI	ACTAGT	1 cut(s)	512
BfaI	CTAG	1 cut(s)	513
BglII	AGATCT	2 cut(s)	74, 966
BisI	GCNGC	2 cut(s)	589, 781
BlsI	GCNGC	2 cut(s)	590, 782
Bme1390I	CCNGG	6 cut(s)	336, 417, 438, 615, 1029, 1030
BmeT110I	CYCGRG	1 cut(s)	1028
BmgT120I	GGNCC	4 cut(s)	323, 638, 990, 991
BmiI	GGNNCC	5 cut(s)	123, 282, 444, 991, 992
BmrFI	CCNGG	6 cut(s)	336, 417, 438, 615, 1029, 1030
BmrI	ACTGGG	1 cut(s)	394
BmsI	GCATC	3 cut(s)	232, 482, 1014
BmuI	ACTGGG	1 cut(s)	394
BpiI	GAAGAC	1 cut(s)	675
BplI	GAGNNNNNCTC	2 cut(s)	720, 752
BpmI	CTGGAG	1 cut(s)	357
BpuEI	CTTGAG	3 cut(s)	257, 839, 998
BpuMI	CCSGG	3 cut(s)	417, 1029, 1030
Bsa29I	ATCGAT	1 cut(s)	568
BsaJI	CCNNGG	4 cut(s)	416, 437, 799, 1028
BsaWI	WCCGGW	1 cut(s)	876
Bsc4I	CCNNNNNNNGG	5 cut(s)	418, 554, 728, 805, 1050
Bse1I	ACTGG	3 cut(s)	389, 709, 715
Bse21I	CCTNAGG	1 cut(s)	276
Bse3DI	GCAATG	1 cut(s)	1121
BseBI	CCWGG	3 cut(s)	336, 438, 615
BseCI	ATCGAT	1 cut(s)	568
BseDI	CCNNGG	4 cut(s)	416, 437, 799, 1028
BseGI	GGATG	2 cut(s)	247, 408
BseLI	CCNNNNNNNGG	5 cut(s)	418, 554, 728, 805, 1050
BseMI	GCAATG	1 cut(s)	1121
BseMII	CTCAG	3 cut(s)	35, 152, 540
BseNI	ACTGG	3 cut(s)	389, 709, 715
BseRI	GAGGAG	4 cut(s)	216, 933, 965, 1097
BseSI	GKGCMC	1 cut(s)	994
BseXI	GCAGC	1 cut(s)	600
BseYI	CCCAGC	1 cut(s)	1077
BshFI	GGCC	5 cut(s)	324, 640, 783, 809, 992
BshVI	ATCGAT	1 cut(s)	568
BsiHKCI	CYCGRG	1 cut(s)	1028
BsiSI	CCGG	3 cut(s)	416, 877, 1029
BslFI	GGGAC	1 cut(s)	168
BslI	CCNNNNNNNGG	5 cut(s)	418, 554, 728, 805, 1050
BsmAI	GTCTC	5 cut(s)	9, 335, 483, 545, 827
BsmFI	GGGAC	1 cut(s)	168
BsmI	GAATGC	1 cut(s)	174
BsnI	GGCC	5 cut(s)	324, 640, 783, 809, 992
BsoBI	CYCGRG	1 cut(s)	1028
Bsp120I	GGGCCC	1 cut(s)	990
Bsp1286I	GDGCHC	5 cut(s)	124, 255, 445, 994, 1014
Bsp143I	GATC	7 cut(s)	74, 361, 565, 569, 630, 651, 966
BspACI	CCGC	5 cut(s)	47, 446, 780, 1008, 1058
BspANI	GGCC	5 cut(s)	324, 640, 783, 809, 992
BspCNI	CTCAG	3 cut(s)	34, 153, 541
BspDI	ATCGAT	1 cut(s)	568
BspLI	GGNNCC	5 cut(s)	123, 282, 444, 991, 992
BspPI	GGATC	2 cut(s)	564, 573
BsrDI	GCAATG	1 cut(s)	1121
BsrI	ACTGG	3 cut(s)	389, 709, 715
BssECI	CCNNGG	4 cut(s)	416, 437, 799, 1028
BssMI	GATC	7 cut(s)	74, 361, 565, 569, 630, 651, 966
BssT1I	CCWWGG	1 cut(s)	799
Bst2UI	CCWGG	3 cut(s)	336, 438, 615
Bst4CI	ACNGT	3 cut(s)	37, 43, 959
Bst6I	CTCTTC	1 cut(s)	1043
BstAPI	GCANNNNNTGC	1 cut(s)	180
BstC8I	GCNNGC	3 cut(s)	642, 646, 1010
BstDEI	CTNAG	5 cut(s)	21, 161, 276, 549, 893
BstEII	GGTNACC	1 cut(s)	731
BstENI	CCTNNNNNAGG	2 cut(s)	552, 1048
BstF5I	GGATG	2 cut(s)	247, 408
BstHHI	GCGC	2 cut(s)	174, 208
BstKTI	GATC	7 cut(s)	77, 364, 568, 572, 633, 654, 969
BstMAI	GTCTC	5 cut(s)	9, 335, 483, 545, 827
BstMBI	GATC	7 cut(s)	74, 361, 565, 569, 630, 651, 966
BstMWI	GCNNNNNNNGC	3 cut(s)	180, 280, 1096
BstNI	CCWGG	3 cut(s)	336, 438, 615
BstNSI	RCATGY	3 cut(s)	187, 748, 1124
BstPI	GGTNACC	1 cut(s)	731
BstSCI	CCNGG	6 cut(s)	334, 415, 436, 613, 1027, 1028
BstSLI	GKGCMC	1 cut(s)	994
BstV1I	GCAGC	1 cut(s)	600
BstV2I	GAAGAC	1 cut(s)	675
BstX2I	RGATCY	2 cut(s)	74, 966
BstYI	RGATCY	2 cut(s)	74, 966
Bsu15I	ATCGAT	1 cut(s)	568
Bsu36I	CCTNAGG	1 cut(s)	276
BsuRI	GGCC	5 cut(s)	324, 640, 783, 809, 992
BsuTUI	ATCGAT	1 cut(s)	568
BtsCI	GGATG	2 cut(s)	247, 408
BtsIMutI	CAGTG	5 cut(s)	29, 195, 708, 813, 964
Cac8I	GCNNGC	3 cut(s)	642, 646, 1010
CaiI	CAGNNNCTG	1 cut(s)	641
CfoI	GCGC	2 cut(s)	174, 208
Cfr13I	GGNCC	4 cut(s)	323, 638, 990, 991
Cfr9I	CCCGGG	1 cut(s)	1028
ClaI	ATCGAT	1 cut(s)	568
CsiI	ACCWGGT	1 cut(s)	613
Csp6I	GTAC	3 cut(s)	724, 880, 955
CviAII	CATG	8 cut(s)	117, 184, 365, 655, 745, 751, 789, 1121
CviQI	GTAC	3 cut(s)	724, 880, 955
DdeI	CTNAG	5 cut(s)	21, 161, 276, 549, 893
DpnI	GATC	7 cut(s)	76, 363, 567, 571, 632, 653, 968
DpnII	GATC	7 cut(s)	74, 361, 565, 569, 630, 651, 966
DraIII	CACNNNGTG	1 cut(s)	95
EaeI	YGGCCR	1 cut(s)	807
Eam1104I	CTCTTC	1 cut(s)	1043
EarI	CTCTTC	1 cut(s)	1043
Eco130I	CCWWGG	1 cut(s)	799
Eco24I	GRGCYC	5 cut(s)	124, 255, 445, 994, 1014
Eco57I	CTGAAG	1 cut(s)	687
Eco81I	CCTNAGG	1 cut(s)	276
Eco88I	CYCGRG	1 cut(s)	1028
Eco91I	GGTNACC	1 cut(s)	731
EcoNI	CCTNNNNNAGG	2 cut(s)	552, 1048
EcoO109I	RGGNCCY	2 cut(s)	638, 990
EcoO65I	GGTNACC	1 cut(s)	731
EcoRI	GAATTC	1 cut(s)	839
EcoRII	CCWGG	3 cut(s)	334, 436, 613
EcoT14I	CCWWGG	1 cut(s)	799
EcoT22I	ATGCAT	1 cut(s)	185
EcoT38I	GRGCYC	5 cut(s)	124, 255, 445, 994, 1014
ErhI	CCWWGG	1 cut(s)	799
FaeI	CATG	8 cut(s)	120, 187, 368, 658, 748, 754, 792, 1124
FaqI	GGGAC	1 cut(s)	168
FatI	CATG	8 cut(s)	116, 183, 364, 654, 744, 750, 788, 1120
FauI	CCCGC	1 cut(s)	1001
FbaI	TGATCA	1 cut(s)	361
FblI	GTMKAC	1 cut(s)	1131
Fnu4HI	GCNGC	2 cut(s)	589, 781
FokI	GGATG	2 cut(s)	254, 395
FriOI	GRGCYC	5 cut(s)	124, 255, 445, 994, 1014
Fsp4HI	GCNGC	2 cut(s)	589, 781
FspBI	CTAG	1 cut(s)	513
GlaI	GCGC	2 cut(s)	173, 207
GluI	GCNGC	2 cut(s)	589, 781
GsaI	CCCAGC	1 cut(s)	1081
GsuI	CTGGAG	1 cut(s)	357
HaeIII	GGCC	5 cut(s)	324, 640, 783, 809, 992
HapII	CCGG	3 cut(s)	416, 877, 1029
HhaI	GCGC	2 cut(s)	174, 208
Hin1II	CATG	8 cut(s)	120, 187, 368, 658, 748, 754, 792, 1124
Hin6I	GCGC	2 cut(s)	172, 206
HinP1I	GCGC	2 cut(s)	172, 206
HincII	GTYRAC	1 cut(s)	100
HindII	GTYRAC	1 cut(s)	100
HindIII	AAGCTT	1 cut(s)	1088
HinfI	GANTC	2 cut(s)	755, 1033
HpaI	GTTAAC	1 cut(s)	100
HpaII	CCGG	3 cut(s)	416, 877, 1029
HphI	GGTGA	2 cut(s)	629, 835
Hpy166II	GTNNAC	3 cut(s)	29, 100, 1132
Hpy188I	TCNGA	1 cut(s)	71
Hpy188III	TCNNGA	2 cut(s)	938, 1037
Hpy8I	GTNNAC	3 cut(s)	29, 100, 1132
Hpy99I	CGWCG	2 cut(s)	531, 744
HpyAV	CCTTC	1 cut(s)	383
HpyCH4III	ACNGT	3 cut(s)	37, 43, 959
HpyCH4V	TGCA	8 cut(s)	183, 193, 293, 473, 588, 603, 701, 788
HpyF10VI	GCNNNNNNNGC	3 cut(s)	180, 280, 1096
HpyF3I	CTNAG	5 cut(s)	21, 161, 276, 549, 893
Hsp92II	CATG	8 cut(s)	120, 187, 368, 658, 748, 754, 792, 1124
HspAI	GCGC	2 cut(s)	172, 206
Ksp22I	TGATCA	1 cut(s)	361
KspAI	GTTAAC	1 cut(s)	100
Kzo9I	GATC	7 cut(s)	74, 361, 565, 569, 630, 651, 966
LmnI	GCTCC	5 cut(s)	127, 229, 280, 337, 448
Lsp1109I	GCAGC	1 cut(s)	600
LweI	GCATC	3 cut(s)	232, 482, 1014
MabI	ACCWGGT	1 cut(s)	613
MaeI	CTAG	1 cut(s)	513
MaeIII	GTNAC	3 cut(s)	37, 731, 959
MalI	GATC	7 cut(s)	76, 363, 567, 571, 632, 653, 968
MboI	GATC	7 cut(s)	74, 361, 565, 569, 630, 651, 966
MboII	GAAGA	6 cut(s)	84, 680, 848, 919, 937, 1060
MfeI	CAATTG	1 cut(s)	468
MflI	RGATCY	2 cut(s)	74, 966
MhlI	GDGCHC	5 cut(s)	124, 255, 445, 994, 1014
MlsI	TGGCCA	1 cut(s)	809
MluCI	AATT	5 cut(s)	468, 624, 767, 839, 871
MluNI	TGGCCA	1 cut(s)	809
Mox20I	TGGCCA	1 cut(s)	809
Mph1103I	ATGCAT	1 cut(s)	185
MroXI	GAANNNNTTC	1 cut(s)	759
MscI	TGGCCA	1 cut(s)	809
MseI	TTAA	6 cut(s)	99, 105, 216, 260, 627, 972
MslI	CAYNNNNRTG	2 cut(s)	188, 749
Msp20I	TGGCCA	1 cut(s)	809
MspA1I	CMGCKG	1 cut(s)	1099
MspI	CCGG	3 cut(s)	416, 877, 1029
MspR9I	CCNGG	6 cut(s)	336, 417, 438, 615, 1029, 1030
MunI	CAATTG	1 cut(s)	468
Mva1269I	GAATGC	1 cut(s)	174
MvaI	CCWGG	3 cut(s)	336, 438, 615
MwoI	GCNNNNNNNGC	3 cut(s)	180, 280, 1096
NciI	CCSGG	3 cut(s)	417, 1029, 1030
NdeII	GATC	7 cut(s)	74, 361, 565, 569, 630, 651, 966
NlaIII	CATG	8 cut(s)	120, 187, 368, 658, 748, 754, 792, 1124
NlaIV	GGNNCC	5 cut(s)	123, 282, 444, 991, 992
NmuCI	GTSAC	2 cut(s)	37, 959
NsiI	ATGCAT	1 cut(s)	185
NspI	RCATGY	3 cut(s)	187, 748, 1124
PctI	GAATGC	1 cut(s)	174
PdmI	GAANNNNTTC	1 cut(s)	759
PfeI	GAWTC	2 cut(s)	755, 1033
PfoI	TCCNGGA	1 cut(s)	334
PkrI	GCNGC	2 cut(s)	590, 782
Psp6I	CCWGG	3 cut(s)	334, 436, 613
PspEI	GGTNACC	1 cut(s)	731
PspFI	CCCAGC	1 cut(s)	1077
PspGI	CCWGG	3 cut(s)	334, 436, 613
PspN4I	GGNNCC	5 cut(s)	123, 282, 444, 991, 992
PspOMI	GGGCCC	1 cut(s)	990
PspPI	GGNCC	4 cut(s)	323, 638, 990, 991
PstNI	CAGNNNCTG	1 cut(s)	641
PsuI	RGATCY	2 cut(s)	74, 966
PvuII	CAGCTG	1 cut(s)	1099
RsaI	GTAC	3 cut(s)	725, 881, 956
RsaNI	GTAC	3 cut(s)	724, 880, 955
RseI	CAYNNNNRTG	2 cut(s)	188, 749
SaqAI	TTAA	6 cut(s)	99, 105, 216, 260, 627, 972
SatI	GCNGC	2 cut(s)	589, 781
Sau3AI	GATC	7 cut(s)	74, 361, 565, 569, 630, 651, 966
Sau96I	GGNCC	4 cut(s)	323, 638, 990, 991
ScrFI	CCNGG	6 cut(s)	336, 417, 438, 615, 1029, 1030
SduI	GDGCHC	5 cut(s)	124, 255, 445, 994, 1014
SexAI	ACCWGGT	1 cut(s)	613
SfaNI	GCATC	3 cut(s)	232, 482, 1014
SmaI	CCCGGG	1 cut(s)	1030
SmiMI	CAYNNNNRTG	2 cut(s)	188, 749
SmlI	CTYRAG	3 cut(s)	236, 854, 1013
SmoI	CTYRAG	3 cut(s)	236, 854, 1013
SpeI	ACTAGT	1 cut(s)	512
Sse9I	AATT	5 cut(s)	468, 624, 767, 839, 871
SsiI	CCGC	5 cut(s)	47, 446, 780, 1008, 1058
SspMI	CTAG	1 cut(s)	513
StyD4I	CCNGG	6 cut(s)	334, 415, 436, 613, 1027, 1028
StyI	CCWWGG	1 cut(s)	799
TaaI	ACNGT	3 cut(s)	37, 43, 959
TaqI	TCGA	3 cut(s)	476, 568, 948
TasI	AATT	5 cut(s)	468, 624, 767, 839, 871
TatI	WGTACW	1 cut(s)	954
TauI	GCSGC	1 cut(s)	783
TfiI	GAWTC	2 cut(s)	755, 1033
Tru1I	TTAA	6 cut(s)	99, 105, 216, 260, 627, 972
Tru9I	TTAA	6 cut(s)	99, 105, 216, 260, 627, 972
TscAI	CASTG	5 cut(s)	36, 195, 715, 820, 964
TseFI	GTSAC	2 cut(s)	37, 959
TseI	GCWGC	1 cut(s)	588
Tsp45I	GTSAC	2 cut(s)	37, 959
TspDTI	ATGAA	2 cut(s)	739, 849
TspGWI	ACGGA	1 cut(s)	728
TspMI	CCCGGG	1 cut(s)	1028
TspRI	CASTG	5 cut(s)	36, 195, 715, 820, 964
XagI	CCTNNNNNAGG	2 cut(s)	552, 1048
XapI	RAATTY	2 cut(s)	767, 839
XceI	RCATGY	3 cut(s)	187, 748, 1124
XmaI	CCCGGG	1 cut(s)	1028
XmiI	GTMKAC	1 cut(s)	1131
XmnI	GAANNNNTTC	1 cut(s)	759
XspI	CTAG	1 cut(s)	513
Zsp2I	ATGCAT	1 cut(s)	185

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.