MD05G1273100.v1.1

Ferredoxin-NADP reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0006772
Physical Location & Seq
Reverse (-)
40814413 .. 40817877
3465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1273100.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGTCTCATTGGGCTCTCTCTCAGGGTTCACTGACTGTTCCTGTTGCAAGTGATTTCTCCCTCAGAAGATCTGTGTTCAAGGCACAAAGTGTTAACTTTAATGATAAATCATGGGCACCTATTTTACCTTTGGACTTGAAGACGAAGAATGGGCAACTGAGAAATCAGCGCATTGTATGCATGTCGGTGCAACAAGCTAGTGCACCTAAAGTTAATGTAGCTCCTTTACAACTTGAGGATGCTAATGAACCCCCATTAAATATACACAAGCCTAAGGAACCTTATACTGCAACCATTCTTTCTGTTGAGAGAATTGTTGGCCCAAAGGCTCCTGGTGAGACTTGCCATATTGTTATTGATCATGGTGGTAATGTGCCCTACTGGGAAGGGCAAAGTTATGGTGTAATTCCTCCTGGTGAAAACCCAAAGAAACCTGGGGCTCCGCACAATGTTCGTCTTTACTCAATTGCATCCTCAAGGTATGGAGACTATTTTGATGGCAAGACAACCAGTTTGTGTGTCCGCCGTGCTGTCTACTATGACCCTGAGACAGGAAAGGAGGATCCTTCCAAGAATGGTGTATGCAGCAACTTTTTATGCAACTCCAAACCTGGTGATAAAATTAAGATCACAGGGCCTGCTGGCAAGATCATGCTTTTGCCTGAAGACGACCCAAATGCCACCCACATTATGATCGCCACTGGCACTGGTGTTGCCCCATACAGAGGTTACCTTCGTCGCATGTTCATGGAATCTGTTCCCAAATTCAAGTTTGGCGGCCTTGCATGGTTATTCCTTGGTGTGGCCAACATTGATAGTCTCCTCTATGATGAAGAATTCACCAAGTATCTCAAGGACTACCCTGATAATTTCCGGTATGACATAGCACTTAGCAGAGAACAGAAGAACCAGAGAGGAGGGAAGATGTATGTTCAGGATAAAATTGAGGAGTATAGTGACGAGATCTTTAAGCTACTGGATAATGGGGCCCACATATACTTCTGCGGGCTCAAGGGTATGATGCCCGGGATTCAAGAGACCTTGAAGAGGGTTGCAGTTGAAAGAGGGGAAAGCTGGGAGGAGAAGCTTTCACAACTGAAAAAGAACAAGCAATGGCATGTGGAGGTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

378

Amino Acids

42.37

Weight (kDa)

8.66

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 231 - 345 3.2e-24 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 115
AccI GTMKAC 2 cut(s) 534, 1128
AciI CCGC 4 cut(s) 443, 523, 777, 1005
AclWI GGATC 2 cut(s) 557, 570
AcoI YGGCCR 1 cut(s) 804
AcsI RAATTY 2 cut(s) 764, 836
AcuI CTGAAG 1 cut(s) 684
AdeI CACNNNGTG 1 cut(s) 89
AfiI CCNNNNNNNGG 4 cut(s) 551, 725, 802, 1047
AgsI TTSAA 6 cut(s) 79, 139, 769, 1034, 1045, 1061
AjnI CCWGG 4 cut(s) 331, 412, 433, 610
AluBI AGCT 5 cut(s) 197, 221, 973, 1074, 1087
AluI AGCT 5 cut(s) 197, 221, 973, 1074, 1087
Alw21I GWGCWC 1 cut(s) 205
Alw26I GTCTC 6 cut(s) 9, 332, 480, 542, 824, 1031
Alw44I GTGCAC 1 cut(s) 201
AlwI GGATC 2 cut(s) 557, 570
AlwNI CAGNNNCTG 1 cut(s) 638
Ama87I CYCGRG 1 cut(s) 1025
AoxI GGCC 5 cut(s) 319, 635, 778, 804, 987
ApaI GGGCCC 1 cut(s) 991
ApaLI GTGCAC 1 cut(s) 201
ApeKI GCWGC 1 cut(s) 585
ApoI RAATTY 2 cut(s) 764, 836
ArsI GACNNNNNNTTYG 2 cut(s) 496, 528
Asp700I GAANNNNTTC 1 cut(s) 756
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 4 cut(s) 320, 635, 987, 988
AsuC2I CCSGG 2 cut(s) 1026, 1027
AsuHPI GGTGA 4 cut(s) 347, 428, 626, 832
AvaI CYCGRG 1 cut(s) 1025
AxyI CCTNAGG 1 cut(s) 273
BaeGI GKGCMC 4 cut(s) 118, 205, 378, 991
BalI TGGCCA 1 cut(s) 806
BamHI GGATCC 1 cut(s) 562
BanI GGYRCC 1 cut(s) 115
BanII GRGCYC 4 cut(s) 16, 442, 991, 1011
BbsI GAAGAC 2 cut(s) 146, 672
Bbv12I GWGCWC 1 cut(s) 205
BbvI GCAGC 1 cut(s) 597
BccI CCATC 1 cut(s) 491
BceAI ACGGC 1 cut(s) 510
BcgI CGANNNNNNTGC 4 cut(s) 434, 468, 659, 693
BciT130I CCWGG 4 cut(s) 333, 414, 435, 612
BclI TGATCA 1 cut(s) 358
BcnI CCSGG 2 cut(s) 1026, 1027
BcoDI GTCTC 6 cut(s) 9, 332, 480, 542, 824, 1031
BfaI CTAG 1 cut(s) 198
BglII AGATCT 2 cut(s) 68, 963
BisI GCNGC 2 cut(s) 586, 778
BlsI GCNGC 2 cut(s) 587, 779
Bme1390I CCNGG 6 cut(s) 333, 414, 435, 612, 1026, 1027
BmeT110I CYCGRG 1 cut(s) 1025
BmgT120I GGNCC 4 cut(s) 320, 635, 987, 988
BmiI GGNNCC 7 cut(s) 117, 279, 330, 441, 564, 988, 989
BmrFI CCNGG 6 cut(s) 333, 414, 435, 612, 1026, 1027
BmrI ACTGGG 1 cut(s) 391
BmsI GCATC 3 cut(s) 229, 479, 1011
BmuI ACTGGG 1 cut(s) 391
BpiI GAAGAC 2 cut(s) 146, 672
BpuEI CTTGAG 4 cut(s) 254, 460, 836, 995
BpuMI CCSGG 2 cut(s) 1026, 1027
BsaI GGTCTC 1 cut(s) 1031
BsaJI CCNNGG 3 cut(s) 434, 796, 1025
BsaWI WCCGGW 1 cut(s) 873
Bsc4I CCNNNNNNNGG 4 cut(s) 551, 725, 802, 1047
Bse1I ACTGG 5 cut(s) 386, 510, 706, 712, 981
Bse21I CCTNAGG 1 cut(s) 273
Bse3DI GCAATG 1 cut(s) 1118
BseBI CCWGG 4 cut(s) 333, 414, 435, 612
BseDI CCNNGG 3 cut(s) 434, 796, 1025
BseGI GGATG 2 cut(s) 244, 470
BseLI CCNNNNNNNGG 4 cut(s) 551, 725, 802, 1047
BseMI GCAATG 1 cut(s) 1118
BseMII CTCAG 4 cut(s) 35, 76, 149, 537
BseNI ACTGG 5 cut(s) 386, 510, 706, 712, 981
BseRI GAGGAG 4 cut(s) 812, 930, 962, 1094
BseSI GKGCMC 4 cut(s) 118, 205, 378, 991
BseXI GCAGC 1 cut(s) 597
BseYI CCCAGC 1 cut(s) 1074
BshFI GGCC 5 cut(s) 321, 637, 780, 806, 989
BshNI GGYRCC 1 cut(s) 115
BsiHKAI GWGCWC 1 cut(s) 205
BsiHKCI CYCGRG 1 cut(s) 1025
BsiSI CCGG 2 cut(s) 874, 1026
BslI CCNNNNNNNGG 4 cut(s) 551, 725, 802, 1047
BsmAI GTCTC 6 cut(s) 9, 332, 480, 542, 824, 1031
BsnI GGCC 5 cut(s) 321, 637, 780, 806, 989
Bso31I GGTCTC 1 cut(s) 1031
BsoBI CYCGRG 1 cut(s) 1025
Bsp120I GGGCCC 1 cut(s) 987
Bsp1286I GDGCHC 7 cut(s) 16, 118, 205, 378, 442, 991, 1011
Bsp143I GATC 7 cut(s) 68, 358, 562, 627, 648, 693, 963
BspACI CCGC 4 cut(s) 443, 523, 777, 1005
BspANI GGCC 5 cut(s) 321, 637, 780, 806, 989
BspCNI CTCAG 4 cut(s) 34, 75, 150, 538
BspLI GGNNCC 7 cut(s) 117, 279, 330, 441, 564, 988, 989
BspPI GGATC 2 cut(s) 557, 570
BspT107I GGYRCC 1 cut(s) 115
BspTNI GGTCTC 1 cut(s) 1031
BsrDI GCAATG 1 cut(s) 1118
BsrI ACTGG 5 cut(s) 386, 510, 706, 712, 981
BssECI CCNNGG 3 cut(s) 434, 796, 1025
BssMI GATC 7 cut(s) 68, 358, 562, 627, 648, 693, 963
BssT1I CCWWGG 1 cut(s) 796
Bst2UI CCWGG 4 cut(s) 333, 414, 435, 612
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 1 cut(s) 1040
BstAPI GCANNNNNTGC 1 cut(s) 177
BstC8I GCNNGC 3 cut(s) 639, 643, 1007
BstDEI CTNAG 6 cut(s) 21, 62, 158, 273, 546, 890
BstEII GGTNACC 1 cut(s) 728
BstENI CCTNNNNNAGG 2 cut(s) 549, 1045
BstF5I GGATG 2 cut(s) 244, 470
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 7 cut(s) 71, 361, 565, 630, 651, 696, 966
BstMAI GTCTC 6 cut(s) 9, 332, 480, 542, 824, 1031
BstMBI GATC 7 cut(s) 68, 358, 562, 627, 648, 693, 963
BstMWI GCNNNNNNNGC 1 cut(s) 177
BstNI CCWGG 4 cut(s) 333, 414, 435, 612
BstNSI RCATGY 3 cut(s) 184, 745, 1121
BstPI GGTNACC 1 cut(s) 728
BstSCI CCNGG 6 cut(s) 331, 412, 433, 610, 1024, 1025
BstSLI GKGCMC 4 cut(s) 118, 205, 378, 991
BstV1I GCAGC 1 cut(s) 597
BstV2I GAAGAC 2 cut(s) 146, 672
BstX2I RGATCY 3 cut(s) 68, 562, 963
BstYI RGATCY 3 cut(s) 68, 562, 963
Bsu36I CCTNAGG 1 cut(s) 273
BsuRI GGCC 5 cut(s) 321, 637, 780, 806, 989
BtsCI GGATG 2 cut(s) 244, 470
BtsIMutI CAGTG 3 cut(s) 29, 699, 705
Cac8I GCNNGC 3 cut(s) 639, 643, 1007
CaiI CAGNNNCTG 1 cut(s) 638
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 4 cut(s) 320, 635, 987, 988
Cfr9I CCCGGG 1 cut(s) 1025
CsiI ACCWGGT 1 cut(s) 610
CviAII CATG 8 cut(s) 111, 181, 362, 652, 742, 748, 786, 1118
DdeI CTNAG 6 cut(s) 21, 62, 158, 273, 546, 890
DpnI GATC 7 cut(s) 70, 360, 564, 629, 650, 695, 965
DpnII GATC 7 cut(s) 68, 358, 562, 627, 648, 693, 963
DraIII CACNNNGTG 1 cut(s) 89
EaeI YGGCCR 1 cut(s) 804
Eam1104I CTCTTC 1 cut(s) 1040
EarI CTCTTC 1 cut(s) 1040
EciI GGCGGA 1 cut(s) 512
Eco130I CCWWGG 1 cut(s) 796
Eco24I GRGCYC 4 cut(s) 16, 442, 991, 1011
Eco31I GGTCTC 1 cut(s) 1031
Eco57I CTGAAG 1 cut(s) 684
Eco81I CCTNAGG 1 cut(s) 273
Eco88I CYCGRG 1 cut(s) 1025
Eco91I GGTNACC 1 cut(s) 728
EcoNI CCTNNNNNAGG 2 cut(s) 549, 1045
EcoO109I RGGNCCY 2 cut(s) 635, 987
EcoO65I GGTNACC 1 cut(s) 728
EcoRI GAATTC 1 cut(s) 836
EcoRII CCWGG 4 cut(s) 331, 412, 433, 610
EcoT14I CCWWGG 1 cut(s) 796
EcoT22I ATGCAT 1 cut(s) 182
EcoT38I GRGCYC 4 cut(s) 16, 442, 991, 1011
ErhI CCWWGG 1 cut(s) 796
FaeI CATG 8 cut(s) 114, 184, 365, 655, 745, 751, 789, 1121
FatI CATG 8 cut(s) 110, 180, 361, 651, 741, 747, 785, 1117
FauI CCCGC 1 cut(s) 998
FbaI TGATCA 1 cut(s) 358
FblI GTMKAC 2 cut(s) 534, 1128
Fnu4HI GCNGC 2 cut(s) 586, 778
FokI GGATG 2 cut(s) 251, 457
FriOI GRGCYC 4 cut(s) 16, 442, 991, 1011
Fsp4HI GCNGC 2 cut(s) 586, 778
FspBI CTAG 1 cut(s) 198
GlaI GCGC 1 cut(s) 170
GluI GCNGC 2 cut(s) 586, 778
GsaI CCCAGC 1 cut(s) 1078
HaeIII GGCC 5 cut(s) 321, 637, 780, 806, 989
HapII CCGG 2 cut(s) 874, 1026
HhaI GCGC 1 cut(s) 171
Hin1II CATG 8 cut(s) 114, 184, 365, 655, 745, 751, 789, 1121
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 1085
HinfI GANTC 2 cut(s) 752, 1030
HpaI GTTAAC 1 cut(s) 94
HpaII CCGG 2 cut(s) 874, 1026
HphI GGTGA 4 cut(s) 347, 428, 626, 832
Hpy166II GTNNAC 5 cut(s) 29, 94, 203, 535, 1129
Hpy188I TCNGA 1 cut(s) 65
Hpy188III TCNNGA 2 cut(s) 935, 1034
Hpy8I GTNNAC 5 cut(s) 29, 94, 203, 535, 1129
Hpy99I CGWCG 1 cut(s) 741
HpyAV CCTTC 3 cut(s) 380, 576, 743
HpyCH4III ACNGT 1 cut(s) 37
HpyF10VI GCNNNNNNNGC 1 cut(s) 177
HpyF3I CTNAG 6 cut(s) 21, 62, 158, 273, 546, 890
Hsp92II CATG 8 cut(s) 114, 184, 365, 655, 745, 751, 789, 1121
HspAI GCGC 1 cut(s) 169
Ksp22I TGATCA 1 cut(s) 358
KspAI GTTAAC 1 cut(s) 94
Kzo9I GATC 7 cut(s) 68, 358, 562, 627, 648, 693, 963
LmnI GCTCC 3 cut(s) 226, 334, 445
Lsp1109I GCAGC 1 cut(s) 597
LweI GCATC 3 cut(s) 229, 479, 1011
MabI ACCWGGT 1 cut(s) 610
MaeI CTAG 1 cut(s) 198
MaeIII GTNAC 2 cut(s) 728, 956
MalI GATC 7 cut(s) 70, 360, 564, 629, 650, 695, 965
MboI GATC 7 cut(s) 68, 358, 562, 627, 648, 693, 963
MboII GAAGA 8 cut(s) 78, 151, 157, 677, 845, 916, 934, 1057
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 3 cut(s) 68, 562, 963
MhlI GDGCHC 7 cut(s) 16, 118, 205, 378, 442, 991, 1011
MlsI TGGCCA 1 cut(s) 806
MluCI AATT 8 cut(s) 312, 405, 465, 621, 764, 836, 868, 942
MluNI TGGCCA 1 cut(s) 806
Mox20I TGGCCA 1 cut(s) 806
Mph1103I ATGCAT 1 cut(s) 182
MroXI GAANNNNTTC 1 cut(s) 756
MscI TGGCCA 1 cut(s) 806
MseI TTAA 6 cut(s) 93, 99, 213, 257, 624, 969
MslI CAYNNNNRTG 2 cut(s) 185, 746
Msp20I TGGCCA 1 cut(s) 806
MspI CCGG 2 cut(s) 874, 1026
MspR9I CCNGG 6 cut(s) 333, 414, 435, 612, 1026, 1027
MunI CAATTG 1 cut(s) 465
MvaI CCWGG 4 cut(s) 333, 414, 435, 612
MwoI GCNNNNNNNGC 1 cut(s) 177
NciI CCSGG 2 cut(s) 1026, 1027
NdeII GATC 7 cut(s) 68, 358, 562, 627, 648, 693, 963
NlaIII CATG 8 cut(s) 114, 184, 365, 655, 745, 751, 789, 1121
NlaIV GGNNCC 7 cut(s) 117, 279, 330, 441, 564, 988, 989
NmuCI GTSAC 1 cut(s) 956
NsiI ATGCAT 1 cut(s) 182
NspI RCATGY 3 cut(s) 184, 745, 1121
PdmI GAANNNNTTC 1 cut(s) 756
PfeI GAWTC 2 cut(s) 752, 1030
PkrI GCNGC 2 cut(s) 587, 779
Psp6I CCWGG 4 cut(s) 331, 412, 433, 610
PspEI GGTNACC 1 cut(s) 728
PspFI CCCAGC 1 cut(s) 1074
PspGI CCWGG 4 cut(s) 331, 412, 433, 610
PspN4I GGNNCC 7 cut(s) 117, 279, 330, 441, 564, 988, 989
PspOMI GGGCCC 1 cut(s) 987
PspPI GGNCC 4 cut(s) 320, 635, 987, 988
PstNI CAGNNNCTG 1 cut(s) 638
PsuI RGATCY 3 cut(s) 68, 562, 963
RseI CAYNNNNRTG 2 cut(s) 185, 746
SaqAI TTAA 6 cut(s) 93, 99, 213, 257, 624, 969
SatI GCNGC 2 cut(s) 586, 778
Sau3AI GATC 7 cut(s) 68, 358, 562, 627, 648, 693, 963
Sau96I GGNCC 4 cut(s) 320, 635, 987, 988
ScrFI CCNGG 6 cut(s) 333, 414, 435, 612, 1026, 1027
SduI GDGCHC 7 cut(s) 16, 118, 205, 378, 442, 991, 1011
SexAI ACCWGGT 1 cut(s) 610
SfaNI GCATC 3 cut(s) 229, 479, 1011
SmaI CCCGGG 1 cut(s) 1027
SmiMI CAYNNNNRTG 2 cut(s) 185, 746
SmlI CTYRAG 4 cut(s) 233, 475, 851, 1010
SmoI CTYRAG 4 cut(s) 233, 475, 851, 1010
Sse9I AATT 8 cut(s) 312, 405, 465, 621, 764, 836, 868, 942
SsiI CCGC 4 cut(s) 443, 523, 777, 1005
SspMI CTAG 1 cut(s) 198
StyD4I CCNGG 6 cut(s) 331, 412, 433, 610, 1024, 1025
StyI CCWWGG 1 cut(s) 796
TaaI ACNGT 1 cut(s) 37
TasI AATT 8 cut(s) 312, 405, 465, 621, 764, 836, 868, 942
TauI GCSGC 1 cut(s) 780
TfiI GAWTC 2 cut(s) 752, 1030
Tru1I TTAA 6 cut(s) 93, 99, 213, 257, 624, 969
Tru9I TTAA 6 cut(s) 93, 99, 213, 257, 624, 969
TscAI CASTG 3 cut(s) 36, 706, 712
TseFI GTSAC 1 cut(s) 956
TseI GCWGC 1 cut(s) 585
Tsp45I GTSAC 1 cut(s) 956
TspDTI ATGAA 3 cut(s) 261, 736, 846
TspMI CCCGGG 1 cut(s) 1025
TspRI CASTG 3 cut(s) 36, 706, 712
VneI GTGCAC 1 cut(s) 201
XagI CCTNNNNNAGG 2 cut(s) 549, 1045
XapI RAATTY 2 cut(s) 764, 836
XceI RCATGY 3 cut(s) 184, 745, 1121
XmaI CCCGGG 1 cut(s) 1025
XmiI GTMKAC 2 cut(s) 534, 1128
XmnI GAANNNNTTC 1 cut(s) 756
XspI CTAG 1 cut(s) 198
Zsp2I ATGCAT 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.