MD10G1253200.v1.1

Ferredoxin-NADP reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006772
Physical Location & Seq
Reverse (-)
34577884 .. 34580680
2797 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1253200.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGTCTCATTTGGCTCTCTCTCAGGGTTCACTGACTGTGACTGTTCCGCTTGGAAGTGATTTCTCCCTCCGAAGATCTGTGTTCAAGGCACGAAGTGTTAGCTTTAATGATAAATCATGGACTCCTATTTTACCTTTGGACTTCAAAACGAAGAATGGACGGCTGAGAAATCAGCGCATTCTATGCATGTCAGTGCAACAAGCAAGTGCGCCTAAAGTTAATGTAGCTCCTCTACAACTCGAGGATGCTAATGAGCCCCCATTAAATACATACAAGCCTAAGGAACCTTATACTGCAACCATTCTTTCTGTTGAGAGGATTGTCGGCCCAAATGCTCCTGGAGAGACTTGCCATATTGTGATTGATCATGGTGGTAATGTGCCTTACTGGGAAGGGCAAAGTTATGGTGTAATTCCTCCTGGTGAAAACCCTAAGAAACCTGGGGCTCCGCAAAATGTTCGCCTTTACTCAATTGCATCCACAAGGTATGGAGACTATTTTGATGGCAAGACAACTAGTTTGTGTGTTCGTCGTGCTGTCTATTACGACCCTGAGACAGGAAAGGAGGATCCTTCCAAGAATGGTGTATGCAGCAACTTTTTATGCAACTCCAAACCTGGTGATAAAATTAAGATCACAGGGCCTGCTGGCAAGATCATGCTTTTGCCTGAAGACAACCCAAATGCCACCCACATTATGATTGCCACTGGCACCGGTGTAGCCCCGTACAGAGGTTACCTCCGTCGCATGTTCATGGAATCTGTTCCCAAATTCAAGTTTGGCGGCCTTGCATGGCTATTCCTTGGTGTGGCCAACACTGATAGTCTCCTCTATGATGAAGAATTCACCAAGTATCTCAAGGACTACCCTGATAATTTCCGGTACAACATAGCACTTAGCAGAGAACAGAAGAACCAGAGAGGAGGGAAGATGTATGTTCAGGATAAAATCGAGGAGTACAGTGACGAGATCTTTAAGCTATTGGACAACGGGGCCCACATATACTTCTGCGGGCTCAAGGGAATGATGCCCGGGATTCAAGAAACCTTGAAGAGGGTTGCGGTTGAAAGAGGGGAGAGCTGGGAGGAGAAGCTTTCACAACTGAAAAAGAACAAGCAATGGCATGTGGAAGTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
NAD_binding_1

Protein Analysis

380

Amino Acids

42.6

Weight (kDa)

8.96

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAD_binding_1 PF00175 233 - 347 2.8e-25 Oxidoreductase NAD-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 710
AccI GTMKAC 1 cut(s) 1134
AciI CCGC 5 cut(s) 47, 449, 783, 1011, 1061
AclWI GGATC 2 cut(s) 563, 576
AcoI YGGCCR 1 cut(s) 810
AcsI RAATTY 2 cut(s) 770, 842
AcuI CTGAAG 1 cut(s) 690
AdeI CACNNNGTG 1 cut(s) 95
AfaI GTAC 3 cut(s) 728, 884, 959
AfiI CCNNNNNNNGG 4 cut(s) 557, 731, 808, 1053
AgeI ACCGGT 1 cut(s) 713
AgsI TTSAA 6 cut(s) 85, 145, 775, 1040, 1051, 1067
AhlI ACTAGT 1 cut(s) 515
AjnI CCWGG 4 cut(s) 337, 418, 439, 616
AluBI AGCT 5 cut(s) 102, 227, 979, 1080, 1093
AluI AGCT 5 cut(s) 102, 227, 979, 1080, 1093
Alw26I GTCTC 5 cut(s) 9, 338, 486, 548, 830
AlwI GGATC 2 cut(s) 563, 576
AlwNI CAGNNNCTG 1 cut(s) 644
Ama87I CYCGRG 2 cut(s) 239, 1031
AoxI GGCC 5 cut(s) 325, 641, 784, 810, 993
ApaI GGGCCC 1 cut(s) 997
ApeKI GCWGC 1 cut(s) 591
ApoI RAATTY 2 cut(s) 770, 842
ArsI GACNNNNNNTTYG 2 cut(s) 502, 534
AsiGI ACCGGT 1 cut(s) 713
Asp700I GAANNNNTTC 1 cut(s) 762
AspLEI GCGC 2 cut(s) 177, 211
AspS9I GGNCC 4 cut(s) 326, 641, 993, 994
AsuC2I CCSGG 2 cut(s) 1032, 1033
AsuHPI GGTGA 3 cut(s) 434, 632, 838
AvaI CYCGRG 2 cut(s) 239, 1031
AxyI CCTNAGG 1 cut(s) 279
BaeGI GKGCMC 1 cut(s) 997
BalI TGGCCA 1 cut(s) 812
BamHI GGATCC 1 cut(s) 568
BanI GGYRCC 1 cut(s) 710
BanII GRGCYC 4 cut(s) 258, 448, 997, 1017
BbsI GAAGAC 1 cut(s) 678
BbvI GCAGC 1 cut(s) 603
BccI CCATC 1 cut(s) 497
BceAI ACGGC 1 cut(s) 176
BcgI CGANNNNNNTGC 2 cut(s) 440, 474
BciT130I CCWGG 4 cut(s) 339, 420, 441, 618
BclI TGATCA 1 cut(s) 364
BcnI CCSGG 2 cut(s) 1032, 1033
BcoDI GTCTC 5 cut(s) 9, 338, 486, 548, 830
BcuI ACTAGT 1 cut(s) 515
BfaI CTAG 1 cut(s) 516
BglII AGATCT 2 cut(s) 74, 969
BisI GCNGC 2 cut(s) 592, 784
BlsI GCNGC 2 cut(s) 593, 785
Bme1390I CCNGG 6 cut(s) 339, 420, 441, 618, 1032, 1033
BmeT110I CYCGRG 2 cut(s) 239, 1031
BmgT120I GGNCC 4 cut(s) 326, 641, 993, 994
BmiI GGNNCC 6 cut(s) 285, 447, 570, 712, 994, 995
BmrFI CCNGG 6 cut(s) 339, 420, 441, 618, 1032, 1033
BmrI ACTGGG 1 cut(s) 397
BmsI GCATC 3 cut(s) 235, 485, 1017
BmuI ACTGGG 1 cut(s) 397
BpiI GAAGAC 1 cut(s) 678
BplI GAGNNNNNCTC 2 cut(s) 723, 755
BpmI CTGGAG 1 cut(s) 360
BpuEI CTTGAG 2 cut(s) 842, 1001
BpuMI CCSGG 2 cut(s) 1032, 1033
BsaJI CCNNGG 3 cut(s) 440, 802, 1031
BsaWI WCCGGW 2 cut(s) 713, 879
Bsc4I CCNNNNNNNGG 4 cut(s) 557, 731, 808, 1053
Bse118I RCCGGY 1 cut(s) 713
Bse1I ACTGG 2 cut(s) 392, 712
Bse21I CCTNAGG 1 cut(s) 279
Bse3DI GCAATG 1 cut(s) 1124
BseBI CCWGG 4 cut(s) 339, 420, 441, 618
BseDI CCNNGG 3 cut(s) 440, 802, 1031
BseGI GGATG 2 cut(s) 250, 476
BseLI CCNNNNNNNGG 4 cut(s) 557, 731, 808, 1053
BseMI GCAATG 1 cut(s) 1124
BseMII CTCAG 3 cut(s) 35, 155, 543
BseNI ACTGG 2 cut(s) 392, 712
BseRI GAGGAG 5 cut(s) 219, 818, 936, 968, 1100
BseSI GKGCMC 1 cut(s) 997
BseXI GCAGC 1 cut(s) 603
BseYI CCCAGC 1 cut(s) 1080
BshFI GGCC 5 cut(s) 327, 643, 786, 812, 995
BshNI GGYRCC 1 cut(s) 710
BshTI ACCGGT 1 cut(s) 713
BsiHKCI CYCGRG 2 cut(s) 239, 1031
BsiSI CCGG 3 cut(s) 714, 880, 1032
BslI CCNNNNNNNGG 4 cut(s) 557, 731, 808, 1053
BsmAI GTCTC 5 cut(s) 9, 338, 486, 548, 830
BsmI GAATGC 1 cut(s) 177
BsnI GGCC 5 cut(s) 327, 643, 786, 812, 995
BsoBI CYCGRG 2 cut(s) 239, 1031
Bsp120I GGGCCC 1 cut(s) 993
Bsp1286I GDGCHC 4 cut(s) 258, 448, 997, 1017
Bsp143I GATC 6 cut(s) 74, 364, 568, 633, 654, 969
BspACI CCGC 5 cut(s) 47, 449, 783, 1011, 1061
BspANI GGCC 5 cut(s) 327, 643, 786, 812, 995
BspCNI CTCAG 3 cut(s) 34, 156, 544
BspLI GGNNCC 6 cut(s) 285, 447, 570, 712, 994, 995
BspPI GGATC 2 cut(s) 563, 576
BspT107I GGYRCC 1 cut(s) 710
BsrDI GCAATG 1 cut(s) 1124
BsrFI RCCGGY 1 cut(s) 713
BsrI ACTGG 2 cut(s) 392, 712
BssAI RCCGGY 1 cut(s) 713
BssECI CCNNGG 3 cut(s) 440, 802, 1031
BssMI GATC 6 cut(s) 74, 364, 568, 633, 654, 969
BssT1I CCWWGG 1 cut(s) 802
Bst2UI CCWGG 4 cut(s) 339, 420, 441, 618
Bst4CI ACNGT 3 cut(s) 37, 43, 962
Bst6I CTCTTC 1 cut(s) 1046
BstAPI GCANNNNNTGC 1 cut(s) 183
BstC8I GCNNGC 3 cut(s) 645, 649, 1013
BstDEI CTNAG 6 cut(s) 21, 164, 279, 432, 552, 896
BstEII GGTNACC 1 cut(s) 734
BstENI CCTNNNNNAGG 2 cut(s) 555, 1051
BstF5I GGATG 2 cut(s) 250, 476
BstHHI GCGC 2 cut(s) 177, 211
BstKTI GATC 6 cut(s) 77, 367, 571, 636, 657, 972
BstMAI GTCTC 5 cut(s) 9, 338, 486, 548, 830
BstMBI GATC 6 cut(s) 74, 364, 568, 633, 654, 969
BstMWI GCNNNNNNNGC 1 cut(s) 183
BstNI CCWGG 4 cut(s) 339, 420, 441, 618
BstNSI RCATGY 3 cut(s) 190, 751, 1127
BstPI GGTNACC 1 cut(s) 734
BstSCI CCNGG 6 cut(s) 337, 418, 439, 616, 1030, 1031
BstSLI GKGCMC 1 cut(s) 997
BstV1I GCAGC 1 cut(s) 603
BstV2I GAAGAC 1 cut(s) 678
BstX2I RGATCY 3 cut(s) 74, 568, 969
BstYI RGATCY 3 cut(s) 74, 568, 969
Bsu36I CCTNAGG 1 cut(s) 279
BsuRI GGCC 5 cut(s) 327, 643, 786, 812, 995
BtsCI GGATG 2 cut(s) 250, 476
BtsIMutI CAGTG 5 cut(s) 29, 198, 705, 816, 967
Cac8I GCNNGC 3 cut(s) 645, 649, 1013
CaiI CAGNNNCTG 1 cut(s) 644
CfoI GCGC 2 cut(s) 177, 211
Cfr10I RCCGGY 1 cut(s) 713
Cfr13I GGNCC 4 cut(s) 326, 641, 993, 994
Cfr9I CCCGGG 1 cut(s) 1031
CsiI ACCWGGT 1 cut(s) 616
Csp6I GTAC 3 cut(s) 727, 883, 958
CspAI ACCGGT 1 cut(s) 713
CviAII CATG 8 cut(s) 117, 187, 368, 658, 748, 754, 792, 1124
CviQI GTAC 3 cut(s) 727, 883, 958
DdeI CTNAG 6 cut(s) 21, 164, 279, 432, 552, 896
DpnI GATC 6 cut(s) 76, 366, 570, 635, 656, 971
DpnII GATC 6 cut(s) 74, 364, 568, 633, 654, 969
DraIII CACNNNGTG 1 cut(s) 95
EaeI YGGCCR 1 cut(s) 810
Eam1104I CTCTTC 1 cut(s) 1046
EarI CTCTTC 1 cut(s) 1046
Eco130I CCWWGG 1 cut(s) 802
Eco24I GRGCYC 4 cut(s) 258, 448, 997, 1017
Eco57I CTGAAG 1 cut(s) 690
Eco81I CCTNAGG 1 cut(s) 279
Eco88I CYCGRG 2 cut(s) 239, 1031
Eco91I GGTNACC 1 cut(s) 734
EcoNI CCTNNNNNAGG 2 cut(s) 555, 1051
EcoO109I RGGNCCY 2 cut(s) 641, 993
EcoO65I GGTNACC 1 cut(s) 734
EcoRI GAATTC 1 cut(s) 842
EcoRII CCWGG 4 cut(s) 337, 418, 439, 616
EcoT14I CCWWGG 1 cut(s) 802
EcoT22I ATGCAT 1 cut(s) 188
EcoT38I GRGCYC 4 cut(s) 258, 448, 997, 1017
ErhI CCWWGG 1 cut(s) 802
FaeI CATG 8 cut(s) 120, 190, 371, 661, 751, 757, 795, 1127
FatI CATG 8 cut(s) 116, 186, 367, 657, 747, 753, 791, 1123
FauI CCCGC 1 cut(s) 1004
FbaI TGATCA 1 cut(s) 364
FblI GTMKAC 1 cut(s) 1134
Fnu4HI GCNGC 2 cut(s) 592, 784
FokI GGATG 2 cut(s) 257, 463
FriOI GRGCYC 4 cut(s) 258, 448, 997, 1017
Fsp4HI GCNGC 2 cut(s) 592, 784
FspBI CTAG 1 cut(s) 516
GlaI GCGC 2 cut(s) 176, 210
GluI GCNGC 2 cut(s) 592, 784
GsaI CCCAGC 1 cut(s) 1084
GsuI CTGGAG 1 cut(s) 360
HaeIII GGCC 5 cut(s) 327, 643, 786, 812, 995
HapII CCGG 3 cut(s) 714, 880, 1032
HhaI GCGC 2 cut(s) 177, 211
Hin1II CATG 8 cut(s) 120, 190, 371, 661, 751, 757, 795, 1127
Hin6I GCGC 2 cut(s) 175, 209
HinP1I GCGC 2 cut(s) 175, 209
HindIII AAGCTT 1 cut(s) 1091
HinfI GANTC 3 cut(s) 121, 758, 1036
HpaII CCGG 3 cut(s) 714, 880, 1032
HphI GGTGA 3 cut(s) 434, 632, 838
Hpy166II GTNNAC 2 cut(s) 29, 1135
Hpy188I TCNGA 1 cut(s) 71
Hpy188III TCNNGA 2 cut(s) 941, 1040
Hpy8I GTNNAC 2 cut(s) 29, 1135
Hpy99I CGWCG 2 cut(s) 534, 747
HpyAV CCTTC 2 cut(s) 386, 582
HpyCH4III ACNGT 3 cut(s) 37, 43, 962
HpyCH4V TGCA 7 cut(s) 186, 196, 296, 476, 591, 606, 791
HpyF10VI GCNNNNNNNGC 1 cut(s) 183
HpyF3I CTNAG 6 cut(s) 21, 164, 279, 432, 552, 896
Hsp92II CATG 8 cut(s) 120, 190, 371, 661, 751, 757, 795, 1127
HspAI GCGC 2 cut(s) 175, 209
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 6 cut(s) 74, 364, 568, 633, 654, 969
LmnI GCTCC 3 cut(s) 232, 340, 451
Lsp1109I GCAGC 1 cut(s) 603
LweI GCATC 3 cut(s) 235, 485, 1017
MabI ACCWGGT 1 cut(s) 616
MaeI CTAG 1 cut(s) 516
MaeIII GTNAC 3 cut(s) 37, 734, 962
MalI GATC 6 cut(s) 76, 366, 570, 635, 656, 971
MboI GATC 6 cut(s) 74, 364, 568, 633, 654, 969
MboII GAAGA 7 cut(s) 84, 163, 683, 851, 922, 940, 1063
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 3 cut(s) 74, 568, 969
MhlI GDGCHC 4 cut(s) 258, 448, 997, 1017
MlsI TGGCCA 1 cut(s) 812
MluCI AATT 6 cut(s) 411, 471, 627, 770, 842, 874
MluNI TGGCCA 1 cut(s) 812
MlyI GAGTC 1 cut(s) 115
Mox20I TGGCCA 1 cut(s) 812
Mph1103I ATGCAT 1 cut(s) 188
MroXI GAANNNNTTC 1 cut(s) 762
MscI TGGCCA 1 cut(s) 812
MseI TTAA 5 cut(s) 105, 219, 263, 630, 975
MslI CAYNNNNRTG 2 cut(s) 191, 752
Msp20I TGGCCA 1 cut(s) 812
MspI CCGG 3 cut(s) 714, 880, 1032
MspR9I CCNGG 6 cut(s) 339, 420, 441, 618, 1032, 1033
MunI CAATTG 1 cut(s) 471
Mva1269I GAATGC 1 cut(s) 177
MvaI CCWGG 4 cut(s) 339, 420, 441, 618
MwoI GCNNNNNNNGC 1 cut(s) 183
NciI CCSGG 2 cut(s) 1032, 1033
NdeII GATC 6 cut(s) 74, 364, 568, 633, 654, 969
NlaIII CATG 8 cut(s) 120, 190, 371, 661, 751, 757, 795, 1127
NlaIV GGNNCC 6 cut(s) 285, 447, 570, 712, 994, 995
NmuCI GTSAC 2 cut(s) 37, 962
NsiI ATGCAT 1 cut(s) 188
NspI RCATGY 3 cut(s) 190, 751, 1127
PaeR7I CTCGAG 1 cut(s) 239
PctI GAATGC 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 762
PfeI GAWTC 2 cut(s) 758, 1036
PfoI TCCNGGA 1 cut(s) 337
PinAI ACCGGT 1 cut(s) 713
PkrI GCNGC 2 cut(s) 593, 785
PleI GAGTC 1 cut(s) 115
PpsI GAGTC 1 cut(s) 115
Psp6I CCWGG 4 cut(s) 337, 418, 439, 616
PspEI GGTNACC 1 cut(s) 734
PspFI CCCAGC 1 cut(s) 1080
PspGI CCWGG 4 cut(s) 337, 418, 439, 616
PspN4I GGNNCC 6 cut(s) 285, 447, 570, 712, 994, 995
PspOMI GGGCCC 1 cut(s) 993
PspPI GGNCC 4 cut(s) 326, 641, 993, 994
PspXI VCTCGAGB 1 cut(s) 239
PstNI CAGNNNCTG 1 cut(s) 644
PsuI RGATCY 3 cut(s) 74, 568, 969
RsaI GTAC 3 cut(s) 728, 884, 959
RsaNI GTAC 3 cut(s) 727, 883, 958
RseI CAYNNNNRTG 2 cut(s) 191, 752
SaqAI TTAA 5 cut(s) 105, 219, 263, 630, 975
SatI GCNGC 2 cut(s) 592, 784
Sau3AI GATC 6 cut(s) 74, 364, 568, 633, 654, 969
Sau96I GGNCC 4 cut(s) 326, 641, 993, 994
SchI GAGTC 1 cut(s) 115
ScrFI CCNGG 6 cut(s) 339, 420, 441, 618, 1032, 1033
SduI GDGCHC 4 cut(s) 258, 448, 997, 1017
SexAI ACCWGGT 1 cut(s) 616
SfaNI GCATC 3 cut(s) 235, 485, 1017
Sfr274I CTCGAG 1 cut(s) 239
SgrAI CRCCGGYG 1 cut(s) 713
SlaI CTCGAG 1 cut(s) 239
SmaI CCCGGG 1 cut(s) 1033
SmiMI CAYNNNNRTG 2 cut(s) 191, 752
SmlI CTYRAG 3 cut(s) 239, 857, 1016
SmoI CTYRAG 3 cut(s) 239, 857, 1016
SpeI ACTAGT 1 cut(s) 515
Sse9I AATT 6 cut(s) 411, 471, 627, 770, 842, 874
SsiI CCGC 5 cut(s) 47, 449, 783, 1011, 1061
SspMI CTAG 1 cut(s) 516
StyD4I CCNGG 6 cut(s) 337, 418, 439, 616, 1030, 1031
StyI CCWWGG 1 cut(s) 802
TaaI ACNGT 3 cut(s) 37, 43, 962
TaqI TCGA 2 cut(s) 240, 951
TasI AATT 6 cut(s) 411, 471, 627, 770, 842, 874
TatI WGTACW 1 cut(s) 957
TauI GCSGC 1 cut(s) 786
TfiI GAWTC 2 cut(s) 758, 1036
Tru1I TTAA 5 cut(s) 105, 219, 263, 630, 975
Tru9I TTAA 5 cut(s) 105, 219, 263, 630, 975
TscAI CASTG 5 cut(s) 36, 198, 712, 823, 967
TseFI GTSAC 2 cut(s) 37, 962
TseI GCWGC 1 cut(s) 591
Tsp45I GTSAC 2 cut(s) 37, 962
TspDTI ATGAA 2 cut(s) 742, 852
TspGWI ACGGA 1 cut(s) 731
TspMI CCCGGG 1 cut(s) 1031
TspRI CASTG 5 cut(s) 36, 198, 712, 823, 967
XagI CCTNNNNNAGG 2 cut(s) 555, 1051
XapI RAATTY 2 cut(s) 770, 842
XceI RCATGY 3 cut(s) 190, 751, 1127
XhoI CTCGAG 1 cut(s) 239
XmaI CCCGGG 1 cut(s) 1031
XmiI GTMKAC 1 cut(s) 1134
XmnI GAANNNNTTC 1 cut(s) 762
XspI CTAG 1 cut(s) 516
Zsp2I ATGCAT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.