AT4G08685

Pollen proteins Ole e I like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009997
Physical Location & Seq
Forward (+)
5550404 .. 5551370
967 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G08685.1

Sequence Viewer

Length: 480 bp
ATGTCTAAAGCAGTTCTATTGGTCGCTCTCTGCTTTTTACCGGCTCTAGCCATCGCGGCTAGACCGAACAAGAACCCTTTCGTCGTGCGAGGCCGTGTCTATTGCGACACTTGCCTCGCTGGTTTCGAAACTCCAGCCTCTACTTACATCTCCGGTGCAGTGGTTAGATTGGAATGTAAAGACAGGAGAACAATGGAGTTAACGTATAGCCACGAGGCGAGAACTGACTCGACAGGATCATACAAGATCTTGGTTAACGAGGATCATGATGAGCAGTTCTGTGATGCAATGTTGGTTCGTAGCTCTCAGTTACGGTGTTCCAATGTGTCCCCTGGTCATGACCGTGCCCGTGTGACTCTCACTCGCTTCAACGGTATTGCTTCTGACGACCGTTTTGCTAACAACATGGGATTTCTAAGGGATGCAGCTATGCCGGGTTGTGCGGATATCATGAAGCTATACCAAGAAACCGAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

159

Amino Acids

17.67

Weight (kDa)

6.29

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 29 - 112 1.7e-17 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 56
AciI CCGC 2 cut(s) 56, 443
AclWI GGATC 2 cut(s) 244, 270
AgsI TTSAA 1 cut(s) 370
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 3 cut(s) 303, 428, 457
AluI AGCT 3 cut(s) 303, 428, 457
AlwI GGATC 2 cut(s) 244, 270
AoxI GGCC 1 cut(s) 91
ApeKI GCWGC 1 cut(s) 425
ArsI GACNNNNNNTTYG 2 cut(s) 377, 409
Asp700I GAANNNNTTC 1 cut(s) 77
AsuC2I CCSGG 1 cut(s) 435
AsuII TTCGAA 1 cut(s) 126
BaeGI GKGCMC 1 cut(s) 349
BauI CACGAG 1 cut(s) 212
BbvI GCAGC 1 cut(s) 437
BccI CCATC 1 cut(s) 59
BceAI ACGGC 1 cut(s) 78
BcgI CGANNNNNNTGC 2 cut(s) 377, 411
BciT130I CCWGG 1 cut(s) 333
BcnI CCSGG 1 cut(s) 435
BfaI CTAG 3 cut(s) 47, 60, 478
BglI GCCNNNNNGGC 1 cut(s) 56
BglII AGATCT 1 cut(s) 246
BisI GCNGC 2 cut(s) 57, 426
BlsI GCNGC 2 cut(s) 58, 427
Bme1390I CCNGG 2 cut(s) 333, 435
BmrFI CCNGG 2 cut(s) 333, 435
BmsI GCATC 2 cut(s) 274, 412
BpmI CTGGAG 1 cut(s) 117
Bpu14I TTCGAA 1 cut(s) 126
BpuMI CCSGG 1 cut(s) 435
BsaJI CCNNGG 2 cut(s) 331, 471
BsaWI WCCGGW 1 cut(s) 152
BsaXI ACNNNNNCTCC 2 cut(s) 188, 218
Bse118I RCCGGY 1 cut(s) 40
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 331, 471
BseGI GGATG 1 cut(s) 427
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 320
BseSI GKGCMC 1 cut(s) 349
BseXI GCAGC 1 cut(s) 437
BsgI GTGCAG 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 56
Bsh1285I CGRYCG 1 cut(s) 391
BshFI GGCC 1 cut(s) 93
BsiEI CGRYCG 1 cut(s) 391
BsiSI CCGG 3 cut(s) 41, 153, 434
BslFI GGGAC 1 cut(s) 313
BsmFI GGGAC 1 cut(s) 313
BsnI GGCC 1 cut(s) 93
Bsp119I TTCGAA 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 349
Bsp143I GATC 3 cut(s) 236, 246, 262
BspACI CCGC 2 cut(s) 56, 443
BspANI GGCC 1 cut(s) 93
BspCNI CTCAG 1 cut(s) 319
BspFNI CGCG 1 cut(s) 56
BspHI TCATGA 3 cut(s) 265, 337, 450
BspPI GGATC 2 cut(s) 244, 270
BspT104I TTCGAA 1 cut(s) 126
BsrDI GCAATG 1 cut(s) 294
BsrFI RCCGGY 1 cut(s) 40
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 2 cut(s) 331, 471
BssMI GATC 3 cut(s) 236, 246, 262
BssSI CACGAG 1 cut(s) 212
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 4 cut(s) 315, 344, 374, 392
BstBI TTCGAA 1 cut(s) 126
BstDEI CTNAG 2 cut(s) 306, 416
BstF5I GGATG 1 cut(s) 427
BstFNI CGCG 1 cut(s) 56
BstKTI GATC 3 cut(s) 239, 249, 265
BstMBI GATC 3 cut(s) 236, 246, 262
BstMCI CGRYCG 1 cut(s) 391
BstMWI GCNNNNNNNGC 2 cut(s) 56, 111
BstNI CCWGG 1 cut(s) 333
BstSCI CCNGG 2 cut(s) 331, 433
BstSLI GKGCMC 1 cut(s) 349
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 1 cut(s) 437
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
BsuRI GGCC 1 cut(s) 93
BtgZI GCGATG 1 cut(s) 37
BtsCI GGATG 1 cut(s) 427
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 1 cut(s) 165
CciI TCATGA 3 cut(s) 265, 337, 450
Cfr10I RCCGGY 1 cut(s) 40
CviAII CATG 4 cut(s) 266, 338, 406, 451
CviJI RGCY 9 cut(s) 44, 50, 59, 93, 137, 210, 303, 428, 457
CviKI_1 RGCY 9 cut(s) 44, 50, 59, 93, 137, 210, 303, 428, 457
DdeI CTNAG 2 cut(s) 306, 416
DpnI GATC 3 cut(s) 238, 248, 264
DpnII GATC 3 cut(s) 236, 246, 262
Eco32I GATATC 1 cut(s) 448
EcoRII CCWGG 1 cut(s) 331
EcoRV GATATC 1 cut(s) 448
FaeI CATG 4 cut(s) 269, 341, 409, 454
FaiI YATR 8 cut(s) 207, 241, 267, 339, 407, 431, 452, 460
FaqI GGGAC 1 cut(s) 313
FatI CATG 4 cut(s) 265, 337, 405, 450
Fnu4HI GCNGC 2 cut(s) 57, 426
FokI GGATG 1 cut(s) 434
Fsp4HI GCNGC 2 cut(s) 57, 426
FspBI CTAG 3 cut(s) 47, 60, 478
GluI GCNGC 2 cut(s) 57, 426
GsuI CTGGAG 1 cut(s) 117
HaeIII GGCC 1 cut(s) 93
HapII CCGG 3 cut(s) 41, 153, 434
Hin1II CATG 4 cut(s) 269, 341, 409, 454
HincII GTYRAC 2 cut(s) 201, 256
HindII GTYRAC 2 cut(s) 201, 256
HinfI GANTC 2 cut(s) 227, 355
HpaI GTTAAC 2 cut(s) 201, 256
HpaII CCGG 3 cut(s) 41, 153, 434
Hpy166II GTNNAC 2 cut(s) 201, 256
Hpy188I TCNGA 1 cut(s) 385
Hpy188III TCNNGA 3 cut(s) 266, 338, 451
Hpy8I GTNNAC 2 cut(s) 201, 256
Hpy99I CGWCG 1 cut(s) 86
HpyCH4III ACNGT 4 cut(s) 315, 344, 374, 392
HpyCH4IV ACGT 1 cut(s) 203
HpyCH4V TGCA 3 cut(s) 158, 287, 425
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 111
HpyF3I CTNAG 2 cut(s) 306, 416
HpySE526I ACGT 1 cut(s) 203
Hsp92II CATG 4 cut(s) 269, 341, 409, 454
KspAI GTTAAC 2 cut(s) 201, 256
Kzo9I GATC 3 cut(s) 236, 246, 262
LpnPI CCDG 9 cut(s) 54, 105, 147, 166, 169, 219, 318, 345, 447
Lsp1109I GCAGC 1 cut(s) 437
LweI GCATC 2 cut(s) 274, 412
MaeI CTAG 3 cut(s) 47, 60, 478
MaeII ACGT 1 cut(s) 203
MaeIII GTNAC 2 cut(s) 309, 352
MalI GATC 3 cut(s) 238, 248, 264
MboI GATC 3 cut(s) 236, 246, 262
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 349
MlyI GAGTC 2 cut(s) 221, 349
MnlI CCTC 6 cut(s) 83, 125, 148, 208, 253, 466
MroXI GAANNNNTTC 1 cut(s) 77
MseI TTAA 2 cut(s) 200, 255
MslI CAYNNNNRTG 1 cut(s) 342
MspI CCGG 3 cut(s) 41, 153, 434
MspR9I CCNGG 2 cut(s) 333, 435
MvaI CCWGG 1 cut(s) 333
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 2 cut(s) 56, 111
NciI CCSGG 1 cut(s) 435
NdeII GATC 3 cut(s) 236, 246, 262
NlaIII CATG 4 cut(s) 269, 341, 409, 454
NmuCI GTSAC 1 cut(s) 352
NspV TTCGAA 1 cut(s) 126
PagI TCATGA 3 cut(s) 265, 337, 450
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PdmI GAANNNNTTC 1 cut(s) 77
PkrI GCNGC 2 cut(s) 58, 427
PleI GAGTC 2 cut(s) 221, 349
PpsI GAGTC 2 cut(s) 221, 349
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PsuI RGATCY 1 cut(s) 246
RseI CAYNNNNRTG 1 cut(s) 342
SaqAI TTAA 2 cut(s) 200, 255
SatI GCNGC 2 cut(s) 57, 426
Sau3AI GATC 3 cut(s) 236, 246, 262
SchI GAGTC 2 cut(s) 221, 349
ScrFI CCNGG 2 cut(s) 333, 435
SduI GDGCHC 1 cut(s) 349
SetI ASST 4 cut(s) 206, 305, 430, 459
SfaNI GCATC 2 cut(s) 274, 412
SfuI TTCGAA 1 cut(s) 126
SmiMI CAYNNNNRTG 1 cut(s) 342
SsiI CCGC 2 cut(s) 56, 443
SspMI CTAG 3 cut(s) 47, 60, 478
StyD4I CCNGG 2 cut(s) 331, 433
TaaI ACNGT 4 cut(s) 315, 344, 374, 392
TaiI ACGT 1 cut(s) 206
TaqI TCGA 2 cut(s) 126, 230
TaqII GACCGA 1 cut(s) 79
TauI GCSGC 1 cut(s) 59
Tru1I TTAA 2 cut(s) 200, 255
Tru9I TTAA 2 cut(s) 200, 255
TscAI CASTG 1 cut(s) 165
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 1 cut(s) 425
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 1 cut(s) 467
TspRI CASTG 1 cut(s) 165
XmnI GAANNNNTTC 1 cut(s) 77
XspI CTAG 3 cut(s) 47, 60, 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.