FvH4_5g37940

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009997
Physical Location & Seq
Reverse (-)
27985925 .. 27988358
2434 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g37940.t1

Sequence Viewer

Length: 480 bp
ATGGCCAAGCTTGTTATGCTCTTCGCTCTCTGCGTCCTGCCCGCTCTGGCCGTCGCCACCCGTCCGATGAGGACGCCGTTCACCGTCGAGGGAAAGGTTTACTGCGACAACTGCCGCTTCAGTTACGAGTCCTCCGCCATCACATACATTGCTGGTGCCACGGTTAGAGTGGAGTGCAAAGACAAGTCAGGTGTGTCACTCAGGTACAGCAAAGAAGGAACTACCGACTCAACTGGAACCTACAGGATTGTAGTTGATGAGGACCATGAGGACCAGATCTGTGATGCTATCCTTGTGAGCAGCCCTCAGAAGAGCTGTTCTGAAGCTACCCCAGGGCGTGACCGTGCCCGTGTCATCCTCACTGGCTACAATGGCATTGCATCAAACAACCGTTTTGCCAATGCTATGGGGTTCATGAGGACTGAGGCCCTGTCTGGTTGTGCCCAGGTTTTCAAGCAGATGCAGGAGTTGGATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

160

Amino Acids

17.37

Weight (kDa)

6.57

Isoelectric Point (pI)

27.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 29 - 111 1.7e-18 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 155
AccBSI CCGCTC 1 cut(s) 44
AciI CCGC 3 cut(s) 42, 115, 135
AcoI YGGCCR 2 cut(s) 3, 48
AcuI CTGAAG 2 cut(s) 103, 342
AcyI GRCGYC 1 cut(s) 74
AfaI GTAC 1 cut(s) 206
AgsI TTSAA 1 cut(s) 454
AjnI CCWGG 2 cut(s) 331, 444
AluBI AGCT 3 cut(s) 10, 315, 326
AluI AGCT 3 cut(s) 10, 315, 326
AoxI GGCC 3 cut(s) 3, 48, 426
ApeKI GCWGC 1 cut(s) 300
AspS9I GGNCC 3 cut(s) 262, 271, 427
AsuHPI GGTGA 1 cut(s) 73
AvaII GGWCC 2 cut(s) 262, 271
BaeGI GKGCMC 2 cut(s) 349, 445
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 155
BbvI GCAGC 1 cut(s) 312
BccI CCATC 1 cut(s) 146
BceAI ACGGC 2 cut(s) 35, 61
BciT130I CCWGG 2 cut(s) 333, 446
BfmI CTRYAG 1 cut(s) 241
BglII AGATCT 1 cut(s) 276
BisI GCNGC 2 cut(s) 115, 301
BlsI GCNGC 2 cut(s) 116, 302
Bme1390I CCNGG 2 cut(s) 333, 446
Bme18I GGWCC 2 cut(s) 262, 271
BmgT120I GGNCC 3 cut(s) 262, 271, 427
BmiI GGNNCC 2 cut(s) 157, 238
BmrFI CCNGG 2 cut(s) 333, 446
BmsI GCATC 4 cut(s) 274, 389, 450, 463
BplI GAGNNNNNCTC 2 cut(s) 289, 321
BsaHI GRCGYC 1 cut(s) 74
BsaJI CCNNGG 4 cut(s) 159, 331, 332, 444
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bse1I ACTGG 2 cut(s) 238, 367
Bse3DI GCAATG 2 cut(s) 147, 375
BseBI CCWGG 2 cut(s) 333, 446
BseDI CCNNGG 4 cut(s) 159, 331, 332, 444
BseGI GGATG 2 cut(s) 354, 478
BseMI GCAATG 2 cut(s) 147, 375
BseMII CTCAG 3 cut(s) 214, 320, 414
BseNI ACTGG 2 cut(s) 238, 367
BseSI GKGCMC 2 cut(s) 349, 445
BseXI GCAGC 1 cut(s) 312
BshFI GGCC 3 cut(s) 5, 50, 428
BshNI GGYRCC 1 cut(s) 155
BsnI GGCC 3 cut(s) 5, 50, 428
Bsp1286I GDGCHC 2 cut(s) 349, 445
Bsp143I GATC 1 cut(s) 276
BspACI CCGC 3 cut(s) 42, 115, 135
BspANI GGCC 3 cut(s) 5, 50, 428
BspCNI CTCAG 3 cut(s) 213, 319, 415
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 2 cut(s) 157, 238
BspQI GCTCTTC 2 cut(s) 26, 305
BspT107I GGYRCC 1 cut(s) 155
BsrBI CCGCTC 1 cut(s) 44
BsrDI GCAATG 2 cut(s) 147, 375
BsrI ACTGG 2 cut(s) 238, 367
BssECI CCNNGG 4 cut(s) 159, 331, 332, 444
BssMI GATC 1 cut(s) 276
BssNI GRCGYC 1 cut(s) 74
Bst2UI CCWGG 2 cut(s) 333, 446
Bst4CI ACNGT 4 cut(s) 85, 163, 344, 392
Bst6I CTCTTC 2 cut(s) 26, 305
BstACI GRCGYC 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 4 cut(s) 200, 306, 423, 477
BstDSI CCRYGG 1 cut(s) 159
BstF5I GGATG 2 cut(s) 354, 478
BstKTI GATC 1 cut(s) 279
BstMBI GATC 1 cut(s) 276
BstMWI GCNNNNNNNGC 3 cut(s) 16, 111, 372
BstNI CCWGG 2 cut(s) 333, 446
BstSCI CCNGG 2 cut(s) 331, 444
BstSFI CTRYAG 1 cut(s) 241
BstSLI GKGCMC 2 cut(s) 349, 445
BstV1I GCAGC 1 cut(s) 312
BstX2I RGATCY 1 cut(s) 276
BstXI CCANNNNNNTGG 1 cut(s) 406
BstYI RGATCY 1 cut(s) 276
BsuRI GGCC 3 cut(s) 5, 50, 428
BtgI CCRYGG 1 cut(s) 159
BtsCI GGATG 2 cut(s) 354, 478
BtsIMutI CAGTG 1 cut(s) 360
Cac8I GCNNGC 1 cut(s) 42
CciI TCATGA 1 cut(s) 414
Cfr13I GGNCC 3 cut(s) 262, 271, 427
CseI GACGC 2 cut(s) 22, 82
Csp6I GTAC 1 cut(s) 205
CviAII CATG 2 cut(s) 266, 415
CviJI RGCY 8 cut(s) 5, 10, 50, 303, 315, 326, 366, 428
CviKI_1 RGCY 8 cut(s) 5, 10, 50, 303, 315, 326, 366, 428
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 4 cut(s) 200, 306, 423, 477
DpnI GATC 1 cut(s) 278
DpnII GATC 1 cut(s) 276
EaeI YGGCCR 2 cut(s) 3, 48
Eam1104I CTCTTC 2 cut(s) 26, 305
EarI CTCTTC 2 cut(s) 26, 305
EciI GGCGGA 1 cut(s) 124
Eco47I GGWCC 2 cut(s) 262, 271
Eco57I CTGAAG 2 cut(s) 103, 342
EcoO109I RGGNCCY 1 cut(s) 427
EcoRII CCWGG 2 cut(s) 331, 444
FaeI CATG 2 cut(s) 269, 418
FaiI YATR 5 cut(s) 17, 145, 267, 407, 416
FatI CATG 2 cut(s) 265, 414
FauI CCCGC 1 cut(s) 49
Fnu4HI GCNGC 2 cut(s) 115, 301
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 2 cut(s) 115, 301
GluI GCNGC 2 cut(s) 115, 301
HaeIII GGCC 3 cut(s) 5, 50, 428
HgaI GACGC 2 cut(s) 22, 82
Hin1I GRCGYC 1 cut(s) 74
Hin1II CATG 2 cut(s) 269, 418
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 2 cut(s) 128, 227
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 2 cut(s) 81, 100
Hpy188I TCNGA 3 cut(s) 66, 309, 322
Hpy188III TCNNGA 1 cut(s) 415
Hpy8I GTNNAC 2 cut(s) 81, 100
Hpy99I CGWCG 2 cut(s) 56, 89
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 4 cut(s) 85, 163, 344, 392
HpyCH4V TGCA 3 cut(s) 177, 380, 463
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 111, 372
HpyF3I CTNAG 4 cut(s) 200, 306, 423, 477
Hsp92I GRCGYC 1 cut(s) 74
Hsp92II CATG 2 cut(s) 269, 418
Kzo9I GATC 1 cut(s) 276
LguI GCTCTTC 2 cut(s) 26, 305
Lsp1109I GCAGC 1 cut(s) 312
LweI GCATC 4 cut(s) 274, 389, 450, 463
MaeIII GTNAC 3 cut(s) 122, 195, 338
MalI GATC 1 cut(s) 278
MbiI CCGCTC 1 cut(s) 44
MboI GATC 1 cut(s) 276
MboII GAAGA 2 cut(s) 13, 322
MflI RGATCY 1 cut(s) 276
MhlI GDGCHC 2 cut(s) 349, 445
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 137, 221
MmeI TCCRAC 1 cut(s) 450
MnlI CCTC 9 cut(s) 63, 82, 142, 253, 262, 315, 368, 411, 418
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 2 cut(s) 333, 446
MvaI CCWGG 2 cut(s) 333, 446
MwoI GCNNNNNNNGC 3 cut(s) 16, 111, 372
NdeII GATC 1 cut(s) 276
NlaIII CATG 2 cut(s) 269, 418
NlaIV GGNNCC 2 cut(s) 157, 238
NmuCI GTSAC 2 cut(s) 195, 338
PagI TCATGA 1 cut(s) 414
PasI CCCWGGG 1 cut(s) 332
PciSI GCTCTTC 2 cut(s) 26, 305
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PkrI GCNGC 2 cut(s) 116, 302
PleI GAGTC 2 cut(s) 136, 221
PpsI GAGTC 2 cut(s) 136, 221
Psp6I CCWGG 2 cut(s) 331, 444
PspGI CCWGG 2 cut(s) 331, 444
PspN4I GGNNCC 2 cut(s) 157, 238
PspPI GGNCC 3 cut(s) 262, 271, 427
PsuI RGATCY 1 cut(s) 276
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SapI GCTCTTC 2 cut(s) 26, 305
SatI GCNGC 2 cut(s) 115, 301
Sau3AI GATC 1 cut(s) 276
Sau96I GGNCC 3 cut(s) 262, 271, 427
SchI GAGTC 2 cut(s) 137, 221
ScrFI CCNGG 2 cut(s) 333, 446
SduI GDGCHC 2 cut(s) 349, 445
SetI ASST 8 cut(s) 12, 99, 193, 206, 242, 317, 328, 450
SfaNI GCATC 4 cut(s) 274, 389, 450, 463
SfcI CTRYAG 1 cut(s) 241
SinI GGWCC 2 cut(s) 262, 271
SsiI CCGC 3 cut(s) 42, 115, 135
StyD4I CCNGG 2 cut(s) 331, 444
TaaI ACNGT 4 cut(s) 85, 163, 344, 392
TaqI TCGA 1 cut(s) 87
TauI GCSGC 1 cut(s) 117
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 2 cut(s) 195, 338
TseI GCWGC 1 cut(s) 300
Tsp45I GTSAC 2 cut(s) 195, 338
TspDTI ATGAA 1 cut(s) 403
TspRI CASTG 1 cut(s) 367
VpaK11BI GGWCC 2 cut(s) 262, 271
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.