MD08G1180600.v1.1

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009997
Physical Location & Seq
Reverse (-)
22338687 .. 22339019
333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1180600.v1.1.491

Sequence Viewer

Length: 333 bp
ATGGCGAAATTGGTTCTGCTAATTACTCTGTGCGTGCTGCCAGCTCTGGCTGCCGCCATGCGTCCCATGAAGACCCCGTTCACGGTAGAGGGGAAGGTTTACTGCGACACTTGCCGCGCTAGGTACGAGACCAAAGCCATCACCTACATTGCCGGTGCCAAGGTTAAACTGGAATGCATAGATAAAAACAACATGGCACTCGTGTACACCAAAGAAGGGACAACCGACTCAGCAGGTTGCTATAAGATCCATGTTGCAAATGACCATTTGGACCAGTTCTGTGATGCAGATACAAGCTACTACTATTTTAGTTTCTCTCTGGAAGGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

111

Amino Acids

12.25

Weight (kDa)

6.09

Isoelectric Point (pI)

14.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 29 - 92 3.1e-13 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 224
AccB1I GGYRCC 1 cut(s) 155
AccII CGCG 1 cut(s) 117
AciI CCGC 2 cut(s) 54, 115
AclWI GGATC 1 cut(s) 241
AfaI GTAC 2 cut(s) 125, 206
AfiI CCNNNNNNNGG 2 cut(s) 82, 216
AluBI AGCT 2 cut(s) 44, 297
AluI AGCT 2 cut(s) 44, 297
Alw26I GTCTC 1 cut(s) 122
AlwI GGATC 1 cut(s) 241
ApeKI GCWGC 2 cut(s) 37, 50
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 1 cut(s) 271
AsuHPI GGTGA 1 cut(s) 133
AvaII GGWCC 1 cut(s) 271
BanI GGYRCC 1 cut(s) 155
BauI CACGAG 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 77
BbvI GCAGC 2 cut(s) 24, 37
BccI CCATC 1 cut(s) 146
BcoDI GTCTC 1 cut(s) 122
BfaI CTAG 1 cut(s) 120
BfuAI ACCTGC 1 cut(s) 224
BisI GCNGC 4 cut(s) 38, 51, 54, 115
BlsI GCNGC 4 cut(s) 39, 52, 55, 116
Bme18I GGWCC 1 cut(s) 271
BmgT120I GGNCC 1 cut(s) 271
BmiI GGNNCC 1 cut(s) 157
BmsI GCATC 1 cut(s) 274
BpiI GAAGAC 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 159
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 216
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 174, 274
Bse3DI GCAATG 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 159
BseLI CCNNNNNNNGG 2 cut(s) 82, 216
BseMI GCAATG 1 cut(s) 147
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 2 cut(s) 174, 274
BseXI GCAGC 2 cut(s) 24, 37
Bsh1236I CGCG 1 cut(s) 117
BshNI GGYRCC 1 cut(s) 155
BsiSI CCGG 1 cut(s) 153
BslFI GGGAC 2 cut(s) 48, 232
BslI CCNNNNNNNGG 2 cut(s) 82, 216
BsmAI GTCTC 1 cut(s) 122
BsmFI GGGAC 2 cut(s) 48, 232
BsmI GAATGC 1 cut(s) 179
Bso31I GGTCTC 1 cut(s) 122
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 1 cut(s) 246
BspACI CCGC 2 cut(s) 54, 115
BspCNI CTCAG 1 cut(s) 242
BspFNI CGCG 1 cut(s) 117
BspLI GGNNCC 1 cut(s) 157
BspMI ACCTGC 1 cut(s) 224
BspPI GGATC 1 cut(s) 241
BspT107I GGYRCC 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 147
BsrFI RCCGGY 1 cut(s) 152
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 2 cut(s) 174, 274
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 1 cut(s) 246
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 85
BstAUI TGTACA 1 cut(s) 204
BstC8I GCNNGC 2 cut(s) 35, 42
BstDEI CTNAG 1 cut(s) 229
BstFNI CGCG 1 cut(s) 117
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 1 cut(s) 249
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 1 cut(s) 246
BstMWI GCNNNNNNNGC 2 cut(s) 50, 111
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 2 cut(s) 24, 37
BstV2I GAAGAC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
BveI ACCTGC 1 cut(s) 224
Cac8I GCNNGC 2 cut(s) 35, 42
CfoI GCGC 1 cut(s) 119
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 1 cut(s) 271
CseI GACGC 1 cut(s) 50
Csp6I GTAC 2 cut(s) 124, 205
CviAII CATG 4 cut(s) 58, 67, 193, 251
CviJI RGCY 4 cut(s) 44, 50, 137, 297
CviKI_1 RGCY 4 cut(s) 44, 50, 137, 297
CviQI GTAC 2 cut(s) 124, 205
DdeI CTNAG 1 cut(s) 229
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 1 cut(s) 271
EcoT14I CCWWGG 1 cut(s) 159
EcoT22I ATGCAT 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 4 cut(s) 61, 70, 196, 254
FaiI YATR 6 cut(s) 59, 68, 179, 194, 243, 252
FaqI GGGAC 2 cut(s) 48, 232
FatI CATG 4 cut(s) 57, 66, 192, 250
Fnu4HI GCNGC 4 cut(s) 38, 51, 54, 115
Fsp4HI GCNGC 4 cut(s) 38, 51, 54, 115
FspBI CTAG 1 cut(s) 120
GlaI GCGC 1 cut(s) 118
GluI GCNGC 4 cut(s) 38, 51, 54, 115
HapII CCGG 1 cut(s) 153
HgaI GACGC 1 cut(s) 50
HhaI GCGC 1 cut(s) 119
Hin1II CATG 4 cut(s) 61, 70, 196, 254
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HinfI GANTC 1 cut(s) 227
HpaII CCGG 1 cut(s) 153
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 4 cut(s) 81, 100, 205, 207
Hpy188III TCNNGA 1 cut(s) 320
Hpy8I GTNNAC 4 cut(s) 81, 100, 205, 207
HpyAV CCTTC 3 cut(s) 88, 209, 317
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4V TGCA 3 cut(s) 177, 257, 287
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 111
HpyF3I CTNAG 1 cut(s) 229
Hsp92II CATG 4 cut(s) 61, 70, 196, 254
HspAI GCGC 1 cut(s) 117
Kzo9I GATC 1 cut(s) 246
LpnPI CCDG 7 cut(s) 32, 54, 155, 166, 219, 287, 305
Lsp1109I GCAGC 2 cut(s) 24, 37
LweI GCATC 1 cut(s) 274
MaeI CTAG 1 cut(s) 120
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 1 cut(s) 82
MflI RGATCY 1 cut(s) 246
MluCI AATT 2 cut(s) 8, 21
MlyI GAGTC 1 cut(s) 221
MnlI CCTC 1 cut(s) 82
Mph1103I ATGCAT 1 cut(s) 179
MseI TTAA 1 cut(s) 165
MspI CCGG 1 cut(s) 153
Mva1269I GAATGC 1 cut(s) 179
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 2 cut(s) 50, 111
NdeII GATC 1 cut(s) 246
NlaIII CATG 4 cut(s) 61, 70, 196, 254
NlaIV GGNNCC 1 cut(s) 157
NsiI ATGCAT 1 cut(s) 179
PctI GAATGC 1 cut(s) 179
PkrI GCNGC 4 cut(s) 39, 52, 55, 116
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PspN4I GGNNCC 1 cut(s) 157
PspPI GGNCC 1 cut(s) 271
PsuI RGATCY 1 cut(s) 246
RsaI GTAC 2 cut(s) 125, 206
RsaNI GTAC 2 cut(s) 124, 205
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 4 cut(s) 38, 51, 54, 115
Sau3AI GATC 1 cut(s) 246
Sau96I GGNCC 1 cut(s) 271
SchI GAGTC 1 cut(s) 221
SetI ASST 7 cut(s) 46, 99, 125, 146, 165, 238, 299
SfaNI GCATC 1 cut(s) 274
SinI GGWCC 1 cut(s) 271
Sse9I AATT 2 cut(s) 8, 21
SsiI CCGC 2 cut(s) 54, 115
SspMI CTAG 1 cut(s) 120
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 1 cut(s) 85
TasI AATT 2 cut(s) 8, 21
TatI WGTACW 1 cut(s) 204
TauI GCSGC 2 cut(s) 56, 117
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TseI GCWGC 2 cut(s) 37, 50
TspDTI ATGAA 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 271
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XspI CTAG 1 cut(s) 120
Zsp2I ATGCAT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.