MD04G1098000.v1.1

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009997
Physical Location & Seq
Forward (+)
18211979 .. 18212254
276 bp
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UTR
Exon/CDS
Intron
MD04G1098000.v1.1.491

Sequence Viewer

Length: 276 bp
ATGGCGAAATTGGTTTTGCTAATTGCTCTGTGTAGGCTGCCAACTCTGGCCGCCGCCATGTGTCTCATGAAGACCCTGTTCACGGTGGAGGGGAAGGTTTACTGCGACACTTGCCGTGCTGGGTACGAGAGCAAAGCCATCACTTACATTGCCGGTGCCAAGGTTAGACTGGAATGCAGAGACAAAAGCAACATGGCACTCGTGTACACCAAAGAAGGGACAACCGACTCAGCAGGTTGCTATAAGATCCCGGTCGCAAATGACCATTTGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

92

Amino Acids

9.93

Weight (kDa)

8.55

Isoelectric Point (pI)

16.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 29 - 89 2.6e-13 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 224
AccB1I GGYRCC 1 cut(s) 155
AciI CCGC 2 cut(s) 51, 54
AclWI GGATC 1 cut(s) 241
AcoI YGGCCR 1 cut(s) 48
AfaI GTAC 2 cut(s) 125, 206
AfiI CCNNNNNNNGG 2 cut(s) 82, 216
Alw26I GTCTC 2 cut(s) 68, 174
AlwI GGATC 1 cut(s) 241
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 37
AsuC2I CCSGG 1 cut(s) 251
BanI GGYRCC 1 cut(s) 155
BauI CACGAG 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 77
BbvI GCAGC 1 cut(s) 24
BccI CCATC 1 cut(s) 146
BceAI ACGGC 1 cut(s) 99
BcnI CCSGG 1 cut(s) 251
BcoDI GTCTC 2 cut(s) 68, 174
BfaI CTAG 1 cut(s) 274
BfuAI ACCTGC 1 cut(s) 224
BisI GCNGC 3 cut(s) 38, 51, 54
BlsI GCNGC 3 cut(s) 39, 52, 55
Bme1390I CCNGG 1 cut(s) 251
BmiI GGNNCC 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 251
BpiI GAAGAC 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 251
BsaJI CCNNGG 1 cut(s) 159
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 216
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 1 cut(s) 174
Bse3DI GCAATG 1 cut(s) 147
BseDI CCNNGG 1 cut(s) 159
BseLI CCNNNNNNNGG 2 cut(s) 82, 216
BseMI GCAATG 1 cut(s) 147
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 1 cut(s) 174
BseXI GCAGC 1 cut(s) 24
BseYI CCCAGC 1 cut(s) 119
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 1 cut(s) 50
BshNI GGYRCC 1 cut(s) 155
BsiEI CGRYCG 1 cut(s) 255
BsiSI CCGG 2 cut(s) 153, 251
BslFI GGGAC 1 cut(s) 232
BslI CCNNNNNNNGG 2 cut(s) 82, 216
BsmAI GTCTC 2 cut(s) 68, 174
BsmFI GGGAC 1 cut(s) 232
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 1 cut(s) 50
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 1 cut(s) 246
BspACI CCGC 2 cut(s) 51, 54
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 242
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 1 cut(s) 157
BspMI ACCTGC 1 cut(s) 224
BspPI GGATC 1 cut(s) 241
BspT107I GGYRCC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 147
BsrFI RCCGGY 1 cut(s) 152
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 1 cut(s) 174
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 1 cut(s) 159
BssMI GATC 1 cut(s) 246
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 200
Bst4CI ACNGT 1 cut(s) 85
BstAUI TGTACA 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 229
BstKTI GATC 1 cut(s) 249
BstMAI GTCTC 2 cut(s) 68, 174
BstMBI GATC 1 cut(s) 246
BstMCI CGRYCG 1 cut(s) 255
BstMWI GCNNNNNNNGC 1 cut(s) 111
BstSCI CCNGG 1 cut(s) 249
BstV1I GCAGC 1 cut(s) 24
BstV2I GAAGAC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
BsuRI GGCC 1 cut(s) 50
BveI ACCTGC 1 cut(s) 224
CciI TCATGA 1 cut(s) 66
Cfr10I RCCGGY 1 cut(s) 152
Csp6I GTAC 2 cut(s) 124, 205
CviAII CATG 3 cut(s) 58, 67, 193
CviJI RGCY 3 cut(s) 37, 50, 137
CviKI_1 RGCY 3 cut(s) 37, 50, 137
CviQI GTAC 2 cut(s) 124, 205
DdeI CTNAG 1 cut(s) 229
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
EaeI YGGCCR 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 159
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 3 cut(s) 61, 70, 196
FaiI YATR 4 cut(s) 59, 68, 194, 243
FaqI GGGAC 1 cut(s) 232
FatI CATG 3 cut(s) 57, 66, 192
Fnu4HI GCNGC 3 cut(s) 38, 51, 54
Fsp4HI GCNGC 3 cut(s) 38, 51, 54
FspBI CTAG 1 cut(s) 274
GluI GCNGC 3 cut(s) 38, 51, 54
GsaI CCCAGC 1 cut(s) 123
HaeIII GGCC 1 cut(s) 50
HapII CCGG 2 cut(s) 153, 251
Hin1II CATG 3 cut(s) 61, 70, 196
HinfI GANTC 1 cut(s) 227
HpaII CCGG 2 cut(s) 153, 251
Hpy166II GTNNAC 4 cut(s) 81, 100, 205, 207
Hpy188III TCNNGA 1 cut(s) 67
Hpy8I GTNNAC 4 cut(s) 81, 100, 205, 207
HpyAV CCTTC 2 cut(s) 88, 209
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4V TGCA 1 cut(s) 177
HpyF10VI GCNNNNNNNGC 1 cut(s) 111
HpyF3I CTNAG 1 cut(s) 229
Hsp92II CATG 3 cut(s) 61, 70, 196
Kzo9I GATC 1 cut(s) 246
LpnPI CCDG 7 cut(s) 32, 89, 105, 155, 166, 219, 264
Lsp1109I GCAGC 1 cut(s) 24
MaeI CTAG 1 cut(s) 274
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 1 cut(s) 82
MflI RGATCY 1 cut(s) 246
MluCI AATT 2 cut(s) 8, 21
MlyI GAGTC 1 cut(s) 221
MnlI CCTC 1 cut(s) 82
MspI CCGG 2 cut(s) 153, 251
MspR9I CCNGG 1 cut(s) 251
Mva1269I GAATGC 1 cut(s) 179
MwoI GCNNNNNNNGC 1 cut(s) 111
NciI CCSGG 1 cut(s) 251
NdeII GATC 1 cut(s) 246
NlaIII CATG 3 cut(s) 61, 70, 196
NlaIV GGNNCC 1 cut(s) 157
PagI TCATGA 1 cut(s) 66
PctI GAATGC 1 cut(s) 179
PkrI GCNGC 3 cut(s) 39, 52, 55
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PspFI CCCAGC 1 cut(s) 119
PspN4I GGNNCC 1 cut(s) 157
PsuI RGATCY 1 cut(s) 246
RsaI GTAC 2 cut(s) 125, 206
RsaNI GTAC 2 cut(s) 124, 205
SatI GCNGC 3 cut(s) 38, 51, 54
Sau3AI GATC 1 cut(s) 246
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 1 cut(s) 251
SetI ASST 3 cut(s) 99, 165, 238
Sse9I AATT 2 cut(s) 8, 21
SsiI CCGC 2 cut(s) 51, 54
SspMI CTAG 1 cut(s) 274
StyD4I CCNGG 1 cut(s) 249
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 1 cut(s) 85
TasI AATT 2 cut(s) 8, 21
TatI WGTACW 1 cut(s) 204
TauI GCSGC 2 cut(s) 53, 56
TseI GCWGC 1 cut(s) 37
TspDTI ATGAA 1 cut(s) 83
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XspI CTAG 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.