MD10G1148500.v1.1

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009997
Physical Location & Seq
Forward (+)
23607089 .. 23607457
369 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1148500.v1.1.491

Sequence Viewer

Length: 309 bp
ATGGCGAAATTGGTTCTGCTAATTGCTCTGTGTGTGTTGTCGGCTCTGGCCGCAGCCACGGGTCCCATGAAGACCCCGTTCACGGTGGAGGGGAAGGTCTACTGCGACACTTGCTGCGCTGGGTATGAGACCAAAGCCATCACCTACATTGTCGGTGCCAAGGTTAGACTGGAATGTAGAGACAAAAGCAGCATGGCACTCGTGTACACCAAAGAAGGAACAACCGACTCAGCAGGTTGCTATAAGATCCTGGTCGCAGAGGACCATTTGGACCAGTTCTGTGATGCAGATACAAGATCGAGCTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

103

Amino Acids

11.01

Weight (kDa)

5.72

Isoelectric Point (pI)

17.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 29 - 96 3.6e-12 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 224
AccB1I GGYRCC 1 cut(s) 155
AccI GTMKAC 1 cut(s) 99
AciI CCGC 1 cut(s) 51
AclWI GGATC 1 cut(s) 241
AcoI YGGCCR 1 cut(s) 48
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 1 cut(s) 82
AjnI CCWGG 1 cut(s) 249
AluBI AGCT 1 cut(s) 303
AluI AGCT 1 cut(s) 303
Alw26I GTCTC 2 cut(s) 122, 174
AlwI GGATC 1 cut(s) 241
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 3 cut(s) 53, 114, 189
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 3 cut(s) 62, 262, 271
AsuHPI GGTGA 1 cut(s) 133
AvaII GGWCC 3 cut(s) 62, 262, 271
BanI GGYRCC 1 cut(s) 155
BauI CACGAG 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 77
BbvI GCAGC 3 cut(s) 65, 101, 201
BccI CCATC 1 cut(s) 146
BcgI CGANNNNNNTGC 2 cut(s) 181, 215
BciT130I CCWGG 1 cut(s) 251
BcoDI GTCTC 2 cut(s) 122, 174
BfuAI ACCTGC 1 cut(s) 224
BisI GCNGC 4 cut(s) 51, 54, 115, 190
BlsI GCNGC 4 cut(s) 52, 55, 116, 191
Bme1390I CCNGG 1 cut(s) 251
Bme18I GGWCC 3 cut(s) 62, 262, 271
BmgT120I GGNCC 3 cut(s) 62, 262, 271
BmiI GGNNCC 3 cut(s) 63, 64, 157
BmrFI CCNGG 1 cut(s) 251
BmsI GCATC 1 cut(s) 274
BpiI GAAGAC 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 2 cut(s) 57, 159
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse1I ACTGG 2 cut(s) 174, 274
BseBI CCWGG 1 cut(s) 251
BseDI CCNNGG 2 cut(s) 57, 159
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 2 cut(s) 174, 274
BseXI GCAGC 3 cut(s) 65, 101, 201
BseYI CCCAGC 1 cut(s) 119
BshFI GGCC 1 cut(s) 50
BshNI GGYRCC 1 cut(s) 155
BslFI GGGAC 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 82
BsmAI GTCTC 2 cut(s) 122, 174
BsmFI GGGAC 1 cut(s) 48
BsnI GGCC 1 cut(s) 50
Bso31I GGTCTC 1 cut(s) 122
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 2 cut(s) 246, 296
BspACI CCGC 1 cut(s) 51
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 242
BspLI GGNNCC 3 cut(s) 63, 64, 157
BspMI ACCTGC 1 cut(s) 224
BspPI GGATC 1 cut(s) 241
BspT107I GGYRCC 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 122
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 2 cut(s) 174, 274
BssECI CCNNGG 2 cut(s) 57, 159
BssMI GATC 2 cut(s) 246, 296
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 251
Bst4CI ACNGT 1 cut(s) 85
BstAUI TGTACA 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 229
BstDSI CCRYGG 1 cut(s) 57
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 2 cut(s) 249, 299
BstMAI GTCTC 2 cut(s) 122, 174
BstMBI GATC 2 cut(s) 246, 296
BstMWI GCNNNNNNNGC 2 cut(s) 50, 111
BstNI CCWGG 1 cut(s) 251
BstSCI CCNGG 1 cut(s) 249
BstV1I GCAGC 3 cut(s) 65, 101, 201
BstV2I GAAGAC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 246
BstYI RGATCY 1 cut(s) 246
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 57
BveI ACCTGC 1 cut(s) 224
CfoI GCGC 1 cut(s) 119
Cfr13I GGNCC 3 cut(s) 62, 262, 271
Csp6I GTAC 1 cut(s) 205
CviAII CATG 2 cut(s) 67, 193
CviJI RGCY 5 cut(s) 44, 50, 56, 137, 303
CviKI_1 RGCY 5 cut(s) 44, 50, 56, 137, 303
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 1 cut(s) 229
DpnI GATC 2 cut(s) 248, 298
DpnII GATC 2 cut(s) 246, 296
EaeI YGGCCR 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 159
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 3 cut(s) 62, 262, 271
EcoO109I RGGNCCY 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 2 cut(s) 70, 196
FaiI YATR 4 cut(s) 68, 126, 194, 243
FaqI GGGAC 1 cut(s) 48
FatI CATG 2 cut(s) 66, 192
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 4 cut(s) 51, 54, 115, 190
Fsp4HI GCNGC 4 cut(s) 51, 54, 115, 190
GlaI GCGC 1 cut(s) 118
GluI GCNGC 4 cut(s) 51, 54, 115, 190
GsaI CCCAGC 1 cut(s) 123
HaeIII GGCC 1 cut(s) 50
HhaI GCGC 1 cut(s) 119
Hin1II CATG 2 cut(s) 70, 196
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HinfI GANTC 1 cut(s) 227
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 4 cut(s) 81, 100, 205, 207
Hpy8I GTNNAC 4 cut(s) 81, 100, 205, 207
HpyAV CCTTC 2 cut(s) 88, 209
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4V TGCA 1 cut(s) 287
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 111
HpyF3I CTNAG 1 cut(s) 229
Hsp92II CATG 2 cut(s) 70, 196
HspAI GCGC 1 cut(s) 117
KflI GGGWCCC 1 cut(s) 62
Kzo9I GATC 2 cut(s) 246, 296
LpnPI CCDG 8 cut(s) 32, 105, 155, 219, 236, 263, 287, 289
Lsp1109I GCAGC 3 cut(s) 65, 101, 201
LweI GCATC 1 cut(s) 274
MalI GATC 2 cut(s) 248, 298
MboI GATC 2 cut(s) 246, 296
MboII GAAGA 1 cut(s) 82
MflI RGATCY 1 cut(s) 246
MluCI AATT 2 cut(s) 8, 21
MlyI GAGTC 1 cut(s) 221
MnlI CCTC 2 cut(s) 82, 253
MspR9I CCNGG 1 cut(s) 251
MvaI CCWGG 1 cut(s) 251
MwoI GCNNNNNNNGC 2 cut(s) 50, 111
NdeII GATC 2 cut(s) 246, 296
NlaIII CATG 2 cut(s) 70, 196
NlaIV GGNNCC 3 cut(s) 63, 64, 157
PkrI GCNGC 4 cut(s) 52, 55, 116, 191
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
PpuMI RGGWCCY 1 cut(s) 62
Psp5II RGGWCCY 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 249
PspFI CCCAGC 1 cut(s) 119
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 3 cut(s) 63, 64, 157
PspPI GGNCC 3 cut(s) 62, 262, 271
PspPPI RGGWCCY 1 cut(s) 62
PsuI RGATCY 1 cut(s) 246
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SatI GCNGC 4 cut(s) 51, 54, 115, 190
Sau3AI GATC 2 cut(s) 246, 296
Sau96I GGNCC 3 cut(s) 62, 262, 271
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 1 cut(s) 251
SetI ASST 5 cut(s) 99, 146, 165, 238, 305
SfaNI GCATC 1 cut(s) 274
SinI GGWCC 3 cut(s) 62, 262, 271
Sse9I AATT 2 cut(s) 8, 21
SsiI CCGC 1 cut(s) 51
StyD4I CCNGG 1 cut(s) 249
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 1 cut(s) 85
TaqI TCGA 1 cut(s) 299
TasI AATT 2 cut(s) 8, 21
TatI WGTACW 1 cut(s) 204
TauI GCSGC 1 cut(s) 53
TseI GCWGC 3 cut(s) 53, 114, 189
TspDTI ATGAA 1 cut(s) 83
VpaK11BI GGWCC 3 cut(s) 62, 262, 271
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XmiI GTMKAC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.