MD13G1213300.v1.1

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0009997
Physical Location & Seq
Forward (+)
20054685 .. 20054951
267 bp
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UTR
Exon/CDS
Intron
MD13G1213300.v1.1.491

Sequence Viewer

Length: 267 bp
ATGAAGACCCCGTTCACAGTAGAGGGGAAAGTCTACTGCGACACTTGCCACGCTGGGTCCGAGACCAAAGCCATCACCTACATTGCCGGTGCCAAGGTTAGACTGGAATGCAAAGACAAAAGCAGCATGGCACTCATGTACACCAAAGAAGGGACAACCGACTCAGCAAGTTGCCATAAGATCCCGGTCCCAGAGGACCATTTGGACCAGTTCTGTGATGCAGATACAAGCTGCTACTGTTTTAGTTTCTCTCTGCAAGGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

89

Amino Acids

9.67

Weight (kDa)

5.25

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 7 - 79 6.5e-12 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 89
AccI GTMKAC 1 cut(s) 33
AclWI GGATC 1 cut(s) 175
AfaI GTAC 1 cut(s) 140
AfiI CCNNNNNNNGG 1 cut(s) 150
AluBI AGCT 1 cut(s) 231
AluI AGCT 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 56
AlwI GGATC 1 cut(s) 175
ApeKI GCWGC 2 cut(s) 123, 231
AspS9I GGNCC 4 cut(s) 57, 187, 196, 205
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 67
AvaII GGWCC 4 cut(s) 57, 187, 196, 205
BanI GGYRCC 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 11
BbvI GCAGC 2 cut(s) 135, 218
BccI CCATC 1 cut(s) 80
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 56
BisI GCNGC 2 cut(s) 124, 232
BlsI GCNGC 2 cut(s) 125, 233
Bme1390I CCNGG 1 cut(s) 185
Bme18I GGWCC 4 cut(s) 57, 187, 196, 205
BmgT120I GGNCC 4 cut(s) 57, 187, 196, 205
BmiI GGNNCC 3 cut(s) 58, 91, 189
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 1 cut(s) 208
BpiI GAAGAC 1 cut(s) 11
BpuMI CCSGG 1 cut(s) 185
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 1 cut(s) 93
Bsc4I CCNNNNNNNGG 1 cut(s) 150
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 108, 208
Bse3DI GCAATG 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 93
BseLI CCNNNNNNNGG 1 cut(s) 150
BseMI GCAATG 1 cut(s) 81
BseMII CTCAG 1 cut(s) 177
BseNI ACTGG 2 cut(s) 108, 208
BseXI GCAGC 2 cut(s) 135, 218
BseYI CCCAGC 1 cut(s) 53
BshNI GGYRCC 1 cut(s) 89
BsiSI CCGG 2 cut(s) 87, 185
BslFI GGGAC 2 cut(s) 166, 173
BslI CCNNNNNNNGG 1 cut(s) 150
BsmAI GTCTC 1 cut(s) 56
BsmFI GGGAC 2 cut(s) 166, 173
BsmI GAATGC 1 cut(s) 113
Bso31I GGTCTC 1 cut(s) 56
Bsp1407I TGTACA 1 cut(s) 138
Bsp143I GATC 1 cut(s) 180
BspCNI CTCAG 1 cut(s) 176
BspLI GGNNCC 3 cut(s) 58, 91, 189
BspPI GGATC 1 cut(s) 175
BspT107I GGYRCC 1 cut(s) 89
BspTNI GGTCTC 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 81
BsrFI RCCGGY 1 cut(s) 86
BsrGI TGTACA 1 cut(s) 138
BsrI ACTGG 2 cut(s) 108, 208
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 93
Bst4CI ACNGT 2 cut(s) 19, 239
BstAUI TGTACA 1 cut(s) 138
BstDEI CTNAG 1 cut(s) 163
BstKTI GATC 1 cut(s) 183
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 1 cut(s) 180
BstMWI GCNNNNNNNGC 1 cut(s) 45
BstSCI CCNGG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 135, 218
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 4 cut(s) 57, 187, 196, 205
Csp6I GTAC 1 cut(s) 139
CviAII CATG 2 cut(s) 127, 136
CviJI RGCY 2 cut(s) 71, 231
CviKI_1 RGCY 2 cut(s) 71, 231
CviQI GTAC 1 cut(s) 139
DdeI CTNAG 1 cut(s) 163
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 93
Eco31I GGTCTC 1 cut(s) 56
Eco47I GGWCC 4 cut(s) 57, 187, 196, 205
EcoT14I CCWWGG 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 2 cut(s) 130, 139
FaiI YATR 3 cut(s) 128, 137, 177
FaqI GGGAC 2 cut(s) 166, 173
FatI CATG 2 cut(s) 126, 135
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 2 cut(s) 124, 232
Fsp4HI GCNGC 2 cut(s) 124, 232
GluI GCNGC 2 cut(s) 124, 232
GsaI CCCAGC 1 cut(s) 57
HapII CCGG 2 cut(s) 87, 185
Hin1II CATG 2 cut(s) 130, 139
HinfI GANTC 1 cut(s) 161
HpaII CCGG 2 cut(s) 87, 185
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 3 cut(s) 15, 34, 141
Hpy188I TCNGA 1 cut(s) 61
Hpy8I GTNNAC 3 cut(s) 15, 34, 141
HpyAV CCTTC 1 cut(s) 143
HpyCH4III ACNGT 2 cut(s) 19, 239
HpyCH4V TGCA 3 cut(s) 111, 221, 256
HpyF10VI GCNNNNNNNGC 1 cut(s) 45
HpyF3I CTNAG 1 cut(s) 163
Hsp92II CATG 2 cut(s) 130, 139
Kzo9I GATC 1 cut(s) 180
LpnPI CCDG 6 cut(s) 39, 89, 100, 198, 204, 221
Lsp1109I GCAGC 2 cut(s) 135, 218
LweI GCATC 1 cut(s) 208
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 1 cut(s) 16
MflI RGATCY 1 cut(s) 180
MlyI GAGTC 1 cut(s) 155
MnlI CCTC 2 cut(s) 16, 187
MspI CCGG 2 cut(s) 87, 185
MspR9I CCNGG 1 cut(s) 185
Mva1269I GAATGC 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 45
NciI CCSGG 1 cut(s) 185
NdeII GATC 1 cut(s) 180
NlaIII CATG 2 cut(s) 130, 139
NlaIV GGNNCC 3 cut(s) 58, 91, 189
PcsI WCGNNNNNNNCGW 1 cut(s) 57
PctI GAATGC 1 cut(s) 113
PkrI GCNGC 2 cut(s) 125, 233
PleI GAGTC 1 cut(s) 155
PpsI GAGTC 1 cut(s) 155
PspFI CCCAGC 1 cut(s) 53
PspN4I GGNNCC 3 cut(s) 58, 91, 189
PspPI GGNCC 4 cut(s) 57, 187, 196, 205
PsuI RGATCY 1 cut(s) 180
RsaI GTAC 1 cut(s) 140
RsaNI GTAC 1 cut(s) 139
SatI GCNGC 2 cut(s) 124, 232
Sau3AI GATC 1 cut(s) 180
Sau96I GGNCC 4 cut(s) 57, 187, 196, 205
SchI GAGTC 1 cut(s) 155
ScrFI CCNGG 1 cut(s) 185
SetI ASST 3 cut(s) 80, 99, 233
SfaNI GCATC 1 cut(s) 208
SinI GGWCC 4 cut(s) 57, 187, 196, 205
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 2 cut(s) 19, 239
TatI WGTACW 1 cut(s) 138
TseI GCWGC 2 cut(s) 123, 231
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 4 cut(s) 57, 187, 196, 205
XcmI CCANNNNNNNNNTGG 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.