RLG00000000722

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009997
Physical Location & Seq
Forward (+)
4455680 .. 4456702
1023 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000722

Sequence Viewer

Length: 480 bp
ATGGCGAAGCTTGTGATGCTGTTCGCTCTGTGCGTGCTACCGGCTCTGGCCGTCGCCACCCGTCCGATGAGGACTCCGTTCACCGTCGAAGGAAAGGTCTACTGCGACGCTTGCCGTTTCGGTTACGAGTCCTCCGCCACCACCTACATTGCCGGTGCTAAGGTTCGAGTGGAATGCAAGGACAAGCTGACCATGGCACTCAGGTACAGCAAAGAAGGGACAACTGACTCAACCGGAACCTACAGGATTACAGTTGCAGAGGACCATGAGGACCAGATCTGTGATTCTATCCTTGTGAGCAGCCCTCAGAAGAGCTGTTCAGAAGCTACCCCAGGACGTGACCGTGCTCGTGTCATCCTCACCGGCTACAATGGCATTGCATCATACAACCGTATGGCCAATGCTATGGGGTTCATGAGGACTGAGGCCGTGTCTGGTTGTTCCCAGATTCTCAAGCAGATGCAGGAGTTGGATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

160

Amino Acids

17.42

Weight (kDa)

8.15

Isoelectric Point (pI)

36.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 29 - 111 7.5e-19 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 99
AciI CCGC 1 cut(s) 135
AcoI YGGCCR 2 cut(s) 48, 396
AfaI GTAC 1 cut(s) 206
AjiI CACGTC 1 cut(s) 338
AjnI CCWGG 1 cut(s) 331
AluBI AGCT 4 cut(s) 10, 187, 315, 326
AluI AGCT 4 cut(s) 10, 187, 315, 326
Alw21I GWGCWC 1 cut(s) 349
AoxI GGCC 3 cut(s) 48, 396, 426
ApeKI GCWGC 1 cut(s) 300
ArsI GACNNNNNNTTYG 2 cut(s) 81, 113
AspS9I GGNCC 2 cut(s) 262, 271
AsuHPI GGTGA 2 cut(s) 73, 352
AvaII GGWCC 2 cut(s) 262, 271
BalI TGGCCA 1 cut(s) 398
BauI CACGAG 1 cut(s) 348
Bbv12I GWGCWC 1 cut(s) 349
BbvI GCAGC 1 cut(s) 312
BceAI ACGGC 3 cut(s) 35, 99, 413
BcgI CGANNNNNNTGC 2 cut(s) 156, 190
BciT130I CCWGG 1 cut(s) 333
BfmI CTRYAG 1 cut(s) 241
BglII AGATCT 1 cut(s) 276
BisI GCNGC 1 cut(s) 301
BlsI GCNGC 1 cut(s) 302
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 2 cut(s) 262, 271
BmgBI CACGTC 1 cut(s) 338
BmgT120I GGNCC 2 cut(s) 262, 271
BmiI GGNNCC 1 cut(s) 238
BmrFI CCNGG 1 cut(s) 333
BmsI GCATC 3 cut(s) 6, 389, 450
BplI GAGNNNNNCTC 2 cut(s) 289, 321
Bpu10I CCTNAGC 1 cut(s) 159
BpuEI CTTGAG 1 cut(s) 437
BsaJI CCNNGG 2 cut(s) 192, 331
BsaWI WCCGGW 1 cut(s) 233
BsaXI ACNNNNNCTCC 2 cut(s) 116, 146
Bse118I RCCGGY 3 cut(s) 40, 152, 362
Bse3DI GCAATG 2 cut(s) 147, 375
BseBI CCWGG 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 192, 331
BseGI GGATG 2 cut(s) 354, 478
BseMI GCAATG 2 cut(s) 147, 375
BseMII CTCAG 3 cut(s) 214, 320, 414
BseXI GCAGC 1 cut(s) 312
BshFI GGCC 3 cut(s) 50, 398, 428
BsiHKAI GWGCWC 1 cut(s) 349
BsiSI CCGG 4 cut(s) 41, 153, 234, 363
BslFI GGGAC 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 232
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 3 cut(s) 50, 398, 428
Bsp1286I GDGCHC 1 cut(s) 349
Bsp143I GATC 1 cut(s) 276
Bsp19I CCATGG 1 cut(s) 192
BspACI CCGC 1 cut(s) 135
BspANI GGCC 3 cut(s) 50, 398, 428
BspCNI CTCAG 3 cut(s) 213, 319, 415
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 238
BspQI GCTCTTC 1 cut(s) 305
BsrDI GCAATG 2 cut(s) 147, 375
BsrFI RCCGGY 3 cut(s) 40, 152, 362
BssAI RCCGGY 3 cut(s) 40, 152, 362
BssECI CCNNGG 2 cut(s) 192, 331
BssMI GATC 1 cut(s) 276
BssSI CACGAG 1 cut(s) 348
BssT1I CCWWGG 1 cut(s) 192
Bst2BI CACGAG 1 cut(s) 348
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 4 cut(s) 85, 253, 344, 392
Bst6I CTCTTC 1 cut(s) 305
BstC8I GCNNGC 2 cut(s) 35, 112
BstDEI CTNAG 4 cut(s) 159, 200, 306, 423
BstDSI CCRYGG 1 cut(s) 192
BstF5I GGATG 2 cut(s) 354, 478
BstKTI GATC 1 cut(s) 279
BstMBI GATC 1 cut(s) 276
BstMWI GCNNNNNNNGC 3 cut(s) 16, 111, 372
BstNI CCWGG 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 331
BstSFI CTRYAG 1 cut(s) 241
BstV1I GCAGC 1 cut(s) 312
BstX2I RGATCY 1 cut(s) 276
BstXI CCANNNNNNTGG 1 cut(s) 406
BstYI RGATCY 1 cut(s) 276
BsuRI GGCC 3 cut(s) 50, 398, 428
BtgI CCRYGG 1 cut(s) 192
BtrI CACGTC 1 cut(s) 338
BtsCI GGATG 2 cut(s) 354, 478
Cac8I GCNNGC 2 cut(s) 35, 112
CciI TCATGA 1 cut(s) 414
Cfr10I RCCGGY 3 cut(s) 40, 152, 362
Cfr13I GGNCC 2 cut(s) 262, 271
CseI GACGC 1 cut(s) 116
Csp6I GTAC 1 cut(s) 205
CspCI CAANNNNNGTGG 2 cut(s) 130, 165
CviAII CATG 3 cut(s) 193, 266, 415
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 4 cut(s) 159, 200, 306, 423
DpnI GATC 1 cut(s) 278
DpnII GATC 1 cut(s) 276
EaeI YGGCCR 2 cut(s) 48, 396
Eam1104I CTCTTC 1 cut(s) 305
EarI CTCTTC 1 cut(s) 305
EciI GGCGGA 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 192
Eco47I GGWCC 2 cut(s) 262, 271
EcoRII CCWGG 1 cut(s) 331
EcoT14I CCWWGG 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 192
FaeI CATG 3 cut(s) 196, 269, 418
FaiI YATR 6 cut(s) 194, 267, 385, 395, 407, 416
FaqI GGGAC 1 cut(s) 232
FatI CATG 3 cut(s) 192, 265, 414
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 1 cut(s) 301
FokI GGATG 1 cut(s) 341
Fsp4HI GCNGC 1 cut(s) 301
GluI GCNGC 1 cut(s) 301
HaeIII GGCC 3 cut(s) 50, 398, 428
HapII CCGG 4 cut(s) 41, 153, 234, 363
HgaI GACGC 1 cut(s) 116
Hin1II CATG 3 cut(s) 196, 269, 418
HindIII AAGCTT 1 cut(s) 8
HinfI GANTC 5 cut(s) 73, 128, 227, 284, 448
HpaII CCGG 4 cut(s) 41, 153, 234, 363
HphI GGTGA 2 cut(s) 73, 352
Hpy166II GTNNAC 2 cut(s) 81, 100
Hpy188I TCNGA 3 cut(s) 66, 309, 322
Hpy188III TCNNGA 1 cut(s) 415
Hpy8I GTNNAC 2 cut(s) 81, 100
Hpy99I CGWCG 3 cut(s) 56, 89, 110
HpyAV CCTTC 2 cut(s) 83, 209
HpyCH4III ACNGT 4 cut(s) 85, 253, 344, 392
HpyCH4IV ACGT 1 cut(s) 337
HpyCH4V TGCA 4 cut(s) 177, 257, 380, 463
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 111, 372
HpyF3I CTNAG 4 cut(s) 159, 200, 306, 423
HpySE526I ACGT 1 cut(s) 337
Hsp92II CATG 3 cut(s) 196, 269, 418
Kzo9I GATC 1 cut(s) 276
LguI GCTCTTC 1 cut(s) 305
Lsp1109I GCAGC 1 cut(s) 312
LweI GCATC 3 cut(s) 6, 389, 450
MaeII ACGT 1 cut(s) 337
MaeIII GTNAC 2 cut(s) 122, 338
MalI GATC 1 cut(s) 278
MboI GATC 1 cut(s) 276
MboII GAAGA 1 cut(s) 322
MflI RGATCY 1 cut(s) 276
MhlI GDGCHC 1 cut(s) 349
MlsI TGGCCA 1 cut(s) 398
MluNI TGGCCA 1 cut(s) 398
MlyI GAGTC 3 cut(s) 67, 137, 221
MmeI TCCRAC 1 cut(s) 450
MnlI CCTC 8 cut(s) 63, 142, 253, 262, 315, 368, 411, 418
Mox20I TGGCCA 1 cut(s) 398
MscI TGGCCA 1 cut(s) 398
Msp20I TGGCCA 1 cut(s) 398
MspI CCGG 4 cut(s) 41, 153, 234, 363
MspR9I CCNGG 1 cut(s) 333
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 1 cut(s) 333
MwoI GCNNNNNNNGC 3 cut(s) 16, 111, 372
NcoI CCATGG 1 cut(s) 192
NdeII GATC 1 cut(s) 276
NlaIII CATG 3 cut(s) 196, 269, 418
NlaIV GGNNCC 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 338
PagI TCATGA 1 cut(s) 414
PciSI GCTCTTC 1 cut(s) 305
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PctI GAATGC 1 cut(s) 179
PfeI GAWTC 2 cut(s) 284, 448
PkrI GCNGC 1 cut(s) 302
PleI GAGTC 3 cut(s) 67, 136, 221
PpsI GAGTC 3 cut(s) 67, 136, 221
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 1 cut(s) 238
PspPI GGNCC 2 cut(s) 262, 271
PsuI RGATCY 1 cut(s) 276
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SapI GCTCTTC 1 cut(s) 305
SatI GCNGC 1 cut(s) 301
Sau3AI GATC 1 cut(s) 276
Sau96I GGNCC 2 cut(s) 262, 271
SchI GAGTC 3 cut(s) 67, 137, 221
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 1 cut(s) 349
SfaNI GCATC 3 cut(s) 6, 389, 450
SfcI CTRYAG 1 cut(s) 241
SinI GGWCC 2 cut(s) 262, 271
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
SsiI CCGC 1 cut(s) 135
StyD4I CCNGG 1 cut(s) 331
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 4 cut(s) 85, 253, 344, 392
TaiI ACGT 1 cut(s) 340
TaqI TCGA 2 cut(s) 87, 166
TfiI GAWTC 2 cut(s) 284, 448
TseFI GTSAC 1 cut(s) 338
TseI GCWGC 1 cut(s) 300
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 1 cut(s) 403
TspGWI ACGGA 1 cut(s) 66
VpaK11BI GGWCC 2 cut(s) 262, 271
XmiI GTMKAC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.