MD15G1364600.v1.1

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009997
Physical Location & Seq
Forward (+)
44173243 .. 44175589
2347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1364600.v1.1.491

Sequence Viewer

Length: 480 bp
ATGGCGAAATTGGTTCTGCTAATTGCTCTGTGCGTGCTGCCGGCTCTGGCCGCCGCCACGCGTCCCATGAAGACCCCGTTCACGGTGGAGGGGAAGGTCTACTGCGACACTTGCCGCGCTGGGTACGAGACCAAAGCCATCACCTACATTGCCGGTGCCAAGGTTAGACTGGAATGCAGAGACAAAAGCAGCATGGCACTCGTGTACACCAAAGAAGGGACAACCGACTCAGCTGGTTGCTATAAGATCCCGGTCGCAGAGGACCATTTGGACCAGTTCTGTGATGCAGTCCTTGTTAGCAGCCCTCAGAAGGACTGTGCAACAGTTTCCCCGGGGCGTGAGCGTGCACGGGTCATCCTCACCGGCTACAATGGCATTGCTTCATACACCCGTTTTGCCAATGCAATGGGGTTCATGAGGACCGAGGCCTTGTCTGGTTGCACCCAGATTCTGAAGGAACTGCAGGAGCTCGACGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

160

Amino Acids

17.24

Weight (kDa)

8.09

Isoelectric Point (pI)

29.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 29 - 111 3.7e-18 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 155
AccI GTMKAC 1 cut(s) 99
AccII CGCG 2 cut(s) 61, 117
AciI CCGC 3 cut(s) 51, 54, 115
AclWI GGATC 1 cut(s) 241
AcoI YGGCCR 1 cut(s) 48
AcuI CTGAAG 1 cut(s) 473
AfaI GTAC 2 cut(s) 125, 206
AfiI CCNNNNNNNGG 3 cut(s) 82, 216, 310
AflIII ACRYGT 2 cut(s) 59, 474
AjiI CACGTC 1 cut(s) 475
AluBI AGCT 2 cut(s) 233, 469
AluI AGCT 2 cut(s) 233, 469
Alw21I GWGCWC 2 cut(s) 349, 471
Alw26I GTCTC 2 cut(s) 122, 174
Alw44I GTGCAC 1 cut(s) 345
AlwI GGATC 1 cut(s) 241
AlwNI CAGNNNCTG 1 cut(s) 451
Ama87I CYCGRG 1 cut(s) 331
AoxI GGCC 2 cut(s) 48, 426
ApaLI GTGCAC 1 cut(s) 345
ApeKI GCWGC 3 cut(s) 37, 189, 300
AspLEI GCGC 1 cut(s) 119
AspS9I GGNCC 3 cut(s) 262, 271, 420
AsuC2I CCSGG 3 cut(s) 251, 332, 333
AsuHPI GGTGA 2 cut(s) 133, 352
AvaI CYCGRG 1 cut(s) 331
AvaII GGWCC 3 cut(s) 262, 271, 420
BaeGI GKGCMC 1 cut(s) 349
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 1 cut(s) 471
BauI CACGAG 1 cut(s) 200
BbsI GAAGAC 1 cut(s) 77
Bbv12I GWGCWC 2 cut(s) 349, 471
BbvI GCAGC 3 cut(s) 24, 201, 312
BccI CCATC 1 cut(s) 146
BcgI CGANNNNNNTGC 2 cut(s) 181, 215
BcnI CCSGG 3 cut(s) 251, 332, 333
BcoDI GTCTC 2 cut(s) 122, 174
BfmI CTRYAG 1 cut(s) 461
BisI GCNGC 6 cut(s) 38, 51, 54, 115, 190, 301
BlsI GCNGC 6 cut(s) 39, 52, 55, 116, 191, 302
Bme1390I CCNGG 3 cut(s) 251, 332, 333
Bme18I GGWCC 3 cut(s) 262, 271, 420
BmeT110I CYCGRG 1 cut(s) 331
BmgBI CACGTC 1 cut(s) 475
BmgT120I GGNCC 3 cut(s) 262, 271, 420
BmiI GGNNCC 1 cut(s) 157
BmrFI CCNGG 3 cut(s) 251, 332, 333
BmsI GCATC 1 cut(s) 274
BpiI GAAGAC 1 cut(s) 77
BpuMI CCSGG 3 cut(s) 251, 332, 333
BsaI GGTCTC 1 cut(s) 122
BsaJI CCNNGG 5 cut(s) 159, 330, 331, 332, 423
Bsc4I CCNNNNNNNGG 3 cut(s) 82, 216, 310
Bse118I RCCGGY 3 cut(s) 40, 152, 362
Bse1I ACTGG 2 cut(s) 174, 274
Bse3DI GCAATG 3 cut(s) 147, 375, 411
BseDI CCNNGG 5 cut(s) 159, 330, 331, 332, 423
BseGI GGATG 1 cut(s) 354
BseLI CCNNNNNNNGG 3 cut(s) 82, 216, 310
BseMI GCAATG 3 cut(s) 147, 375, 411
BseMII CTCAG 2 cut(s) 243, 320
BseNI ACTGG 2 cut(s) 174, 274
BseSI GKGCMC 1 cut(s) 349
BseXI GCAGC 3 cut(s) 24, 201, 312
BseYI CCCAGC 1 cut(s) 119
Bsh1236I CGCG 2 cut(s) 61, 117
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 2 cut(s) 50, 428
BshNI GGYRCC 1 cut(s) 155
BsiEI CGRYCG 1 cut(s) 255
BsiHKAI GWGCWC 2 cut(s) 349, 471
BsiHKCI CYCGRG 1 cut(s) 331
BsiSI CCGG 5 cut(s) 41, 153, 251, 332, 363
BslFI GGGAC 2 cut(s) 48, 232
BslI CCNNNNNNNGG 3 cut(s) 82, 216, 310
BsmAI GTCTC 2 cut(s) 122, 174
BsmFI GGGAC 2 cut(s) 48, 232
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 2 cut(s) 50, 428
Bso31I GGTCTC 1 cut(s) 122
BsoBI CYCGRG 1 cut(s) 331
Bsp1286I GDGCHC 2 cut(s) 349, 471
Bsp1407I TGTACA 1 cut(s) 204
Bsp143I GATC 1 cut(s) 246
BspACI CCGC 3 cut(s) 51, 54, 115
BspANI GGCC 2 cut(s) 50, 428
BspCNI CTCAG 2 cut(s) 242, 319
BspFNI CGCG 2 cut(s) 61, 117
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 1 cut(s) 157
BspMAI CTGCAG 1 cut(s) 465
BspPI GGATC 1 cut(s) 241
BspT107I GGYRCC 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 122
BsrDI GCAATG 3 cut(s) 147, 375, 411
BsrFI RCCGGY 3 cut(s) 40, 152, 362
BsrGI TGTACA 1 cut(s) 204
BsrI ACTGG 2 cut(s) 174, 274
BssAI RCCGGY 3 cut(s) 40, 152, 362
BssECI CCNNGG 5 cut(s) 159, 330, 331, 332, 423
BssMI GATC 1 cut(s) 246
BssSI CACGAG 1 cut(s) 200
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 200
Bst4CI ACNGT 3 cut(s) 85, 317, 325
BstAUI TGTACA 1 cut(s) 204
BstC8I GCNNGC 3 cut(s) 35, 42, 345
BstDEI CTNAG 2 cut(s) 229, 306
BstF5I GGATG 1 cut(s) 354
BstFNI CGCG 2 cut(s) 61, 117
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 1 cut(s) 249
BstMAI GTCTC 2 cut(s) 122, 174
BstMBI GATC 1 cut(s) 246
BstMCI CGRYCG 1 cut(s) 255
BstMWI GCNNNNNNNGC 3 cut(s) 50, 111, 372
BstSCI CCNGG 3 cut(s) 249, 330, 331
BstSFI CTRYAG 1 cut(s) 461
BstSLI GKGCMC 1 cut(s) 349
BstUI CGCG 2 cut(s) 61, 117
BstV1I GCAGC 3 cut(s) 24, 201, 312
BstV2I GAAGAC 1 cut(s) 77
BstX2I RGATCY 1 cut(s) 246
BstXI CCANNNNNNTGG 1 cut(s) 406
BstYI RGATCY 1 cut(s) 246
BsuRI GGCC 2 cut(s) 50, 428
BtrI CACGTC 1 cut(s) 475
BtsCI GGATG 1 cut(s) 354
Cac8I GCNNGC 3 cut(s) 35, 42, 345
CaiI CAGNNNCTG 1 cut(s) 451
CciI TCATGA 1 cut(s) 414
CfoI GCGC 1 cut(s) 119
Cfr10I RCCGGY 3 cut(s) 40, 152, 362
Cfr13I GGNCC 3 cut(s) 262, 271, 420
Cfr9I CCCGGG 1 cut(s) 331
CseI GACGC 1 cut(s) 50
Csp6I GTAC 2 cut(s) 124, 205
CviAII CATG 3 cut(s) 67, 193, 415
CviJI RGCY 8 cut(s) 44, 50, 137, 233, 303, 366, 428, 469
CviKI_1 RGCY 8 cut(s) 44, 50, 137, 233, 303, 366, 428, 469
CviQI GTAC 2 cut(s) 124, 205
DdeI CTNAG 2 cut(s) 229, 306
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
EaeI YGGCCR 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 469
Eco130I CCWWGG 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 428
Eco24I GRGCYC 1 cut(s) 471
Eco31I GGTCTC 1 cut(s) 122
Eco47I GGWCC 3 cut(s) 262, 271, 420
Eco53kI GAGCTC 1 cut(s) 469
Eco57I CTGAAG 1 cut(s) 473
Eco88I CYCGRG 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 469
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 471
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 3 cut(s) 70, 196, 418
FaiI YATR 5 cut(s) 68, 194, 243, 385, 416
FaqI GGGAC 2 cut(s) 48, 232
FatI CATG 3 cut(s) 66, 192, 414
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 6 cut(s) 38, 51, 54, 115, 190, 301
FokI GGATG 1 cut(s) 341
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 6 cut(s) 38, 51, 54, 115, 190, 301
GlaI GCGC 1 cut(s) 118
GluI GCNGC 6 cut(s) 38, 51, 54, 115, 190, 301
GsaI CCCAGC 1 cut(s) 123
HaeIII GGCC 2 cut(s) 50, 428
HapII CCGG 5 cut(s) 41, 153, 251, 332, 363
HgaI GACGC 1 cut(s) 50
HhaI GCGC 1 cut(s) 119
Hin1II CATG 3 cut(s) 70, 196, 418
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HinfI GANTC 2 cut(s) 227, 448
HpaII CCGG 5 cut(s) 41, 153, 251, 332, 363
HphI GGTGA 2 cut(s) 133, 352
Hpy166II GTNNAC 5 cut(s) 81, 100, 205, 207, 347
Hpy188I TCNGA 2 cut(s) 309, 453
Hpy188III TCNNGA 1 cut(s) 415
Hpy8I GTNNAC 5 cut(s) 81, 100, 205, 207, 347
Hpy99I CGWCG 1 cut(s) 476
HpyAV CCTTC 4 cut(s) 88, 209, 304, 448
HpyCH4III ACNGT 3 cut(s) 85, 317, 325
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 7 cut(s) 177, 287, 320, 347, 404, 441, 463
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 111, 372
HpyF3I CTNAG 2 cut(s) 229, 306
HpySE526I ACGT 1 cut(s) 474
Hsp92II CATG 3 cut(s) 70, 196, 418
HspAI GCGC 1 cut(s) 117
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
Kzo9I GATC 1 cut(s) 246
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 3 cut(s) 24, 201, 312
LweI GCATC 1 cut(s) 274
MaeII ACGT 1 cut(s) 474
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 1 cut(s) 82
MflI RGATCY 1 cut(s) 246
MhlI GDGCHC 2 cut(s) 349, 471
MluCI AATT 2 cut(s) 8, 21
MluI ACGCGT 1 cut(s) 59
MlyI GAGTC 1 cut(s) 221
MnlI CCTC 6 cut(s) 82, 253, 315, 368, 411, 418
MroNI GCCGGC 1 cut(s) 40
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 5 cut(s) 41, 153, 251, 332, 363
MspR9I CCNGG 3 cut(s) 251, 332, 333
Mva1269I GAATGC 1 cut(s) 179
MvnI CGCG 2 cut(s) 61, 117
MwoI GCNNNNNNNGC 3 cut(s) 50, 111, 372
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 3 cut(s) 251, 332, 333
NdeII GATC 1 cut(s) 246
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 3 cut(s) 70, 196, 418
NlaIV GGNNCC 1 cut(s) 157
PagI TCATGA 1 cut(s) 414
PceI AGGCCT 1 cut(s) 428
PctI GAATGC 1 cut(s) 179
PdiI GCCGGC 1 cut(s) 42
PfeI GAWTC 1 cut(s) 448
PkrI GCNGC 6 cut(s) 39, 52, 55, 116, 191, 302
PleI GAGTC 1 cut(s) 221
PpsI GAGTC 1 cut(s) 221
Psp124BI GAGCTC 1 cut(s) 471
PspFI CCCAGC 1 cut(s) 119
PspN4I GGNNCC 1 cut(s) 157
PspPI GGNCC 3 cut(s) 262, 271, 420
PstI CTGCAG 1 cut(s) 465
PstNI CAGNNNCTG 1 cut(s) 451
PsuI RGATCY 1 cut(s) 246
PvuII CAGCTG 1 cut(s) 233
RsaI GTAC 2 cut(s) 125, 206
RsaNI GTAC 2 cut(s) 124, 205
SacI GAGCTC 1 cut(s) 471
SatI GCNGC 6 cut(s) 38, 51, 54, 115, 190, 301
Sau3AI GATC 1 cut(s) 246
Sau96I GGNCC 3 cut(s) 262, 271, 420
SchI GAGTC 1 cut(s) 221
ScrFI CCNGG 3 cut(s) 251, 332, 333
SduI GDGCHC 2 cut(s) 349, 471
SetI ASST 6 cut(s) 99, 146, 165, 235, 471, 477
SfaNI GCATC 1 cut(s) 274
SfcI CTRYAG 1 cut(s) 461
SinI GGWCC 3 cut(s) 262, 271, 420
SmaI CCCGGG 1 cut(s) 333
Sse9I AATT 2 cut(s) 8, 21
SseBI AGGCCT 1 cut(s) 428
SsiI CCGC 3 cut(s) 51, 54, 115
SstI GAGCTC 1 cut(s) 471
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 3 cut(s) 249, 330, 331
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 85, 317, 325
TaiI ACGT 1 cut(s) 477
TaqI TCGA 1 cut(s) 471
TaqII GACCGA 1 cut(s) 437
TasI AATT 2 cut(s) 8, 21
TatI WGTACW 1 cut(s) 204
TauI GCSGC 3 cut(s) 53, 56, 117
TfiI GAWTC 1 cut(s) 448
TseI GCWGC 3 cut(s) 37, 189, 300
TspDTI ATGAA 3 cut(s) 83, 372, 403
TspMI CCCGGG 1 cut(s) 331
VneI GTGCAC 1 cut(s) 345
VpaK11BI GGWCC 3 cut(s) 262, 271, 420
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XmaI CCCGGG 1 cut(s) 331
XmiI GTMKAC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.