MD08G1178700.v1.1

Pollen-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0009997
Physical Location & Seq
Forward (+)
21980379 .. 21982405
2027 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1178700.v1.1.491

Sequence Viewer

Length: 657 bp
ATGGGCAATCAATTTTTCCGATTATTACTGAGGCTTTTTCCCCACCAACATCATCACACACACACACACTCTCTCTCTCTTCATATCTCTCACTCCTCTGCATTTCATTTCCGCAATCTTGTTATCTCTCTCAAATCTCTCCGCCAAAACGAAGTGAAAAGACAGAAAACGGCTTCAATGGCGAAATTGTTTCTGCTAATTGCTCTGTGCATGCTTCCGGCTCTGGCCGCCGCCACGCGTCCCATGAGGACCCCGTTCACGGTGGAGGGAAAGGTATACTGCGACACTTGCCGCGCTGGGTACGAGACCAAAGCCATCACCTACATTGCTGGTGCCAAGGTTAGACTGGAATGCAGAGACAAGACTAGCATGGCACTCCTGTACACCAAAGAAGGGACAACCGACTCAGCAGGTTGCTATAAGATCCCAGTCGCAGAGGACCATTTGGACCAATTCTGTGATGCAGTCCTTGTTAGCAGCCCTCAGAAGGACTGTGCAACAGTTTCCCCAGGGCGTGAGCGTGCACGTGTCATCCTCACCGCCAACAACGGTATTGCTTCGTACAACCGGTTTGCCAATGCTATGGGTTTCATGAGGACGGAAGCCTTGTCTGGCTGCACCCAGATTCTCAAGGAATTGCAGGAGCTCGACGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

219

Amino Acids

24.38

Weight (kDa)

9.16

Isoelectric Point (pI)

32.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 88 - 169 1.1e-17 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 401
AccB1I GGYRCC 1 cut(s) 332
AccI GTMKAC 1 cut(s) 276
AccII CGCG 2 cut(s) 238, 294
AciI CCGC 6 cut(s) 112, 142, 228, 231, 292, 540
AclWI GGATC 1 cut(s) 418
AcoI YGGCCR 1 cut(s) 225
AcvI CACGTG 1 cut(s) 527
AfaI GTAC 3 cut(s) 302, 383, 563
AfiI CCNNNNNNNGG 3 cut(s) 259, 393, 487
AflIII ACRYGT 2 cut(s) 236, 526
AgeI ACCGGT 1 cut(s) 567
AgsI TTSAA 1 cut(s) 177
AjnI CCWGG 1 cut(s) 508
AluBI AGCT 1 cut(s) 646
AluI AGCT 1 cut(s) 646
Alw21I GWGCWC 2 cut(s) 526, 648
Alw26I GTCTC 2 cut(s) 299, 351
Alw44I GTGCAC 1 cut(s) 522
AlwI GGATC 1 cut(s) 418
AoxI GGCC 1 cut(s) 225
ApaLI GTGCAC 1 cut(s) 522
ApeKI GCWGC 2 cut(s) 477, 615
AsiGI ACCGGT 1 cut(s) 567
AspLEI GCGC 1 cut(s) 296
AspS9I GGNCC 3 cut(s) 249, 439, 448
AsuHPI GGTGA 2 cut(s) 310, 529
AvaII GGWCC 3 cut(s) 249, 439, 448
BaeGI GKGCMC 1 cut(s) 526
BanI GGYRCC 1 cut(s) 332
BanII GRGCYC 1 cut(s) 648
BbrPI CACGTG 1 cut(s) 527
Bbv12I GWGCWC 2 cut(s) 526, 648
BbvI GCAGC 2 cut(s) 489, 602
BccI CCATC 1 cut(s) 323
BceAI ACGGC 1 cut(s) 186
BciT130I CCWGG 1 cut(s) 510
BcoDI GTCTC 2 cut(s) 299, 351
BfaI CTAG 1 cut(s) 366
BfuAI ACCTGC 1 cut(s) 401
BisI GCNGC 5 cut(s) 228, 231, 292, 478, 616
BlsI GCNGC 5 cut(s) 229, 232, 293, 479, 617
Bme1390I CCNGG 1 cut(s) 510
Bme18I GGWCC 3 cut(s) 249, 439, 448
BmgT120I GGNCC 3 cut(s) 249, 439, 448
BmiI GGNNCC 2 cut(s) 251, 334
BmrFI CCNGG 1 cut(s) 510
BmrI ACTGGG 1 cut(s) 422
BmsI GCATC 1 cut(s) 451
BmuI ACTGGG 1 cut(s) 422
BpuEI CTTGAG 1 cut(s) 614
BsaAI YACGTR 1 cut(s) 527
BsaI GGTCTC 1 cut(s) 299
BsaJI CCNNGG 3 cut(s) 336, 508, 509
BsaWI WCCGGW 1 cut(s) 567
Bsc4I CCNNNNNNNGG 3 cut(s) 259, 393, 487
Bse118I RCCGGY 1 cut(s) 567
Bse1I ACTGG 2 cut(s) 351, 428
Bse3DI GCAATG 1 cut(s) 324
BseBI CCWGG 1 cut(s) 510
BseDI CCNNGG 3 cut(s) 336, 508, 509
BseGI GGATG 1 cut(s) 531
BseLI CCNNNNNNNGG 3 cut(s) 259, 393, 487
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 3 cut(s) 20, 420, 497
BseNI ACTGG 2 cut(s) 351, 428
BseRI GAGGAG 1 cut(s) 85
BseSI GKGCMC 1 cut(s) 526
BseXI GCAGC 2 cut(s) 489, 602
BseYI CCCAGC 1 cut(s) 296
BsgI GTGCAG 1 cut(s) 601
Bsh1236I CGCG 2 cut(s) 238, 294
BshFI GGCC 1 cut(s) 227
BshNI GGYRCC 1 cut(s) 332
BshTI ACCGGT 1 cut(s) 567
BsiHKAI GWGCWC 2 cut(s) 526, 648
BsiSI CCGG 2 cut(s) 218, 568
BslFI GGGAC 2 cut(s) 225, 409
BslI CCNNNNNNNGG 3 cut(s) 259, 393, 487
BsmAI GTCTC 2 cut(s) 299, 351
BsmFI GGGAC 2 cut(s) 225, 409
BsmI GAATGC 1 cut(s) 356
BsnI GGCC 1 cut(s) 227
Bso31I GGTCTC 1 cut(s) 299
Bsp1286I GDGCHC 2 cut(s) 526, 648
Bsp1407I TGTACA 1 cut(s) 381
Bsp143I GATC 1 cut(s) 423
BspACI CCGC 6 cut(s) 112, 142, 228, 231, 292, 540
BspANI GGCC 1 cut(s) 227
BspCNI CTCAG 3 cut(s) 21, 419, 496
BspFNI CGCG 2 cut(s) 238, 294
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 2 cut(s) 251, 334
BspMI ACCTGC 1 cut(s) 401
BspPI GGATC 1 cut(s) 418
BspT107I GGYRCC 1 cut(s) 332
BspTNI GGTCTC 1 cut(s) 299
BsrDI GCAATG 1 cut(s) 324
BsrFI RCCGGY 1 cut(s) 567
BsrGI TGTACA 1 cut(s) 381
BsrI ACTGG 2 cut(s) 351, 428
BssAI RCCGGY 1 cut(s) 567
BssECI CCNNGG 3 cut(s) 336, 508, 509
BssMI GATC 1 cut(s) 423
BssNAI GTATAC 1 cut(s) 277
BssT1I CCWWGG 1 cut(s) 336
Bst1107I GTATAC 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 510
Bst4CI ACNGT 4 cut(s) 262, 494, 502, 551
Bst6I CTCTTC 1 cut(s) 84
BstAUI TGTACA 1 cut(s) 381
BstBAI YACGTR 1 cut(s) 527
BstC8I GCNNGC 2 cut(s) 212, 522
BstDEI CTNAG 3 cut(s) 29, 406, 483
BstF5I GGATG 1 cut(s) 531
BstFNI CGCG 2 cut(s) 238, 294
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 1 cut(s) 426
BstMAI GTCTC 2 cut(s) 299, 351
BstMBI GATC 1 cut(s) 423
BstMWI GCNNNNNNNGC 3 cut(s) 179, 227, 288
BstNI CCWGG 1 cut(s) 510
BstNSI RCATGY 1 cut(s) 214
BstSCI CCNGG 1 cut(s) 508
BstSLI GKGCMC 1 cut(s) 526
BstUI CGCG 2 cut(s) 238, 294
BstV1I GCAGC 2 cut(s) 489, 602
BstX2I RGATCY 1 cut(s) 423
BstXI CCANNNNNNTGG 1 cut(s) 583
BstYI RGATCY 1 cut(s) 423
BstZ17I GTATAC 1 cut(s) 277
BsuRI GGCC 1 cut(s) 227
BtsCI GGATG 1 cut(s) 531
BveI ACCTGC 1 cut(s) 401
Cac8I GCNNGC 2 cut(s) 212, 522
CciI TCATGA 1 cut(s) 591
CfoI GCGC 1 cut(s) 296
Cfr10I RCCGGY 1 cut(s) 567
Cfr13I GGNCC 3 cut(s) 249, 439, 448
CseI GACGC 1 cut(s) 227
Csp6I GTAC 3 cut(s) 301, 382, 562
CspAI ACCGGT 1 cut(s) 567
CviAII CATG 4 cut(s) 211, 244, 370, 592
CviJI RGCY 9 cut(s) 34, 173, 221, 227, 314, 480, 605, 615, 646
CviKI_1 RGCY 9 cut(s) 34, 173, 221, 227, 314, 480, 605, 615, 646
CviQI GTAC 3 cut(s) 301, 382, 562
DdeI CTNAG 3 cut(s) 29, 406, 483
DpnI GATC 1 cut(s) 425
DpnII GATC 1 cut(s) 423
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
EciI GGCGGA 1 cut(s) 131
Ecl136II GAGCTC 1 cut(s) 646
Eco130I CCWWGG 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 648
Eco31I GGTCTC 1 cut(s) 299
Eco47I GGWCC 3 cut(s) 249, 439, 448
Eco53kI GAGCTC 1 cut(s) 646
Eco72I CACGTG 1 cut(s) 527
EcoICRI GAGCTC 1 cut(s) 646
EcoO109I RGGNCCY 1 cut(s) 249
EcoRII CCWGG 1 cut(s) 508
EcoT14I CCWWGG 1 cut(s) 336
EcoT38I GRGCYC 1 cut(s) 648
ErhI CCWWGG 1 cut(s) 336
FaeI CATG 4 cut(s) 214, 247, 373, 595
FaiI YATR 8 cut(s) 84, 212, 245, 277, 371, 420, 584, 593
FaqI GGGAC 2 cut(s) 225, 409
FatI CATG 4 cut(s) 210, 243, 369, 591
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 5 cut(s) 228, 231, 292, 478, 616
FokI GGATG 1 cut(s) 518
FriOI GRGCYC 1 cut(s) 648
Fsp4HI GCNGC 5 cut(s) 228, 231, 292, 478, 616
FspBI CTAG 1 cut(s) 366
GlaI GCGC 1 cut(s) 295
GluI GCNGC 5 cut(s) 228, 231, 292, 478, 616
GsaI CCCAGC 1 cut(s) 300
HaeIII GGCC 1 cut(s) 227
HapII CCGG 2 cut(s) 218, 568
HgaI GACGC 1 cut(s) 227
HhaI GCGC 1 cut(s) 296
Hin1II CATG 4 cut(s) 214, 247, 373, 595
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HinfI GANTC 2 cut(s) 404, 625
HpaII CCGG 2 cut(s) 218, 568
HphI GGTGA 2 cut(s) 310, 529
Hpy166II GTNNAC 4 cut(s) 258, 277, 384, 524
Hpy188I TCNGA 2 cut(s) 20, 486
Hpy188III TCNNGA 1 cut(s) 592
Hpy8I GTNNAC 4 cut(s) 258, 277, 384, 524
Hpy99I CGWCG 1 cut(s) 653
HpyAV CCTTC 2 cut(s) 386, 481
HpyCH4III ACNGT 4 cut(s) 262, 494, 502, 551
HpyCH4IV ACGT 1 cut(s) 526
HpyCH4V TGCA 8 cut(s) 101, 210, 354, 464, 497, 524, 618, 640
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 227, 288
HpyF3I CTNAG 3 cut(s) 29, 406, 483
HpySE526I ACGT 1 cut(s) 526
Hsp92II CATG 4 cut(s) 214, 247, 373, 595
HspAI GCGC 1 cut(s) 294
Kzo9I GATC 1 cut(s) 423
LmnI GCTCC 1 cut(s) 643
Lsp1109I GCAGC 2 cut(s) 489, 602
LweI GCATC 1 cut(s) 451
MaeI CTAG 1 cut(s) 366
MaeII ACGT 1 cut(s) 526
MalI GATC 1 cut(s) 425
MboI GATC 1 cut(s) 423
MboII GAAGA 1 cut(s) 71
MflI RGATCY 1 cut(s) 423
MhlI GDGCHC 2 cut(s) 526, 648
MluCI AATT 5 cut(s) 11, 185, 198, 452, 635
MluI ACGCGT 1 cut(s) 236
MlyI GAGTC 1 cut(s) 398
MnlI CCTC 8 cut(s) 24, 106, 240, 259, 430, 492, 545, 588
MspI CCGG 2 cut(s) 218, 568
MspR9I CCNGG 1 cut(s) 510
Mva1269I GAATGC 1 cut(s) 356
MvaI CCWGG 1 cut(s) 510
MvnI CGCG 2 cut(s) 238, 294
MwoI GCNNNNNNNGC 3 cut(s) 179, 227, 288
NdeII GATC 1 cut(s) 423
NlaIII CATG 4 cut(s) 214, 247, 373, 595
NlaIV GGNNCC 2 cut(s) 251, 334
NspI RCATGY 1 cut(s) 214
PaeI GCATGC 1 cut(s) 214
PagI TCATGA 1 cut(s) 591
PasI CCCWGGG 1 cut(s) 509
PctI GAATGC 1 cut(s) 356
PfeI GAWTC 1 cut(s) 625
PinAI ACCGGT 1 cut(s) 567
PkrI GCNGC 5 cut(s) 229, 232, 293, 479, 617
PleI GAGTC 1 cut(s) 398
PmaCI CACGTG 1 cut(s) 527
PmlI CACGTG 1 cut(s) 527
PpsI GAGTC 1 cut(s) 398
Ppu21I YACGTR 1 cut(s) 527
PpuMI RGGWCCY 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 648
Psp5II RGGWCCY 1 cut(s) 249
Psp6I CCWGG 1 cut(s) 508
PspCI CACGTG 1 cut(s) 527
PspFI CCCAGC 1 cut(s) 296
PspGI CCWGG 1 cut(s) 508
PspN4I GGNNCC 2 cut(s) 251, 334
PspPI GGNCC 3 cut(s) 249, 439, 448
PspPPI RGGWCCY 1 cut(s) 249
PsuI RGATCY 1 cut(s) 423
RsaI GTAC 3 cut(s) 302, 383, 563
RsaNI GTAC 3 cut(s) 301, 382, 562
SacI GAGCTC 1 cut(s) 648
SatI GCNGC 5 cut(s) 228, 231, 292, 478, 616
Sau3AI GATC 1 cut(s) 423
Sau96I GGNCC 3 cut(s) 249, 439, 448
SchI GAGTC 1 cut(s) 398
ScrFI CCNGG 1 cut(s) 510
SduI GDGCHC 2 cut(s) 526, 648
SetI ASST 6 cut(s) 276, 323, 342, 415, 529, 648
SfaNI GCATC 1 cut(s) 451
SinI GGWCC 3 cut(s) 249, 439, 448
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
SphI GCATGC 1 cut(s) 214
Sse9I AATT 5 cut(s) 11, 185, 198, 452, 635
SsiI CCGC 6 cut(s) 112, 142, 228, 231, 292, 540
SspMI CTAG 1 cut(s) 366
SstI GAGCTC 1 cut(s) 648
StyD4I CCNGG 1 cut(s) 508
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 4 cut(s) 262, 494, 502, 551
TaiI ACGT 1 cut(s) 529
TaqI TCGA 1 cut(s) 648
TasI AATT 5 cut(s) 11, 185, 198, 452, 635
TatI WGTACW 1 cut(s) 381
TauI GCSGC 3 cut(s) 230, 233, 294
TfiI GAWTC 1 cut(s) 625
TseI GCWGC 2 cut(s) 477, 615
TspDTI ATGAA 3 cut(s) 71, 95, 580
TspGWI ACGGA 1 cut(s) 614
VneI GTGCAC 1 cut(s) 522
VpaK11BI GGWCC 3 cut(s) 249, 439, 448
XceI RCATGY 1 cut(s) 214
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XmiI GTMKAC 1 cut(s) 276
XspI CTAG 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.