AT4G10750

HpcH/HpaI aldolase/citrate lyase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0012383
Physical Location & Seq
Forward (+)
6618676 .. 6619939
1264 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G10750.1

Sequence Viewer

Length: 1077 bp
ATGGCCACCGCTTCAATCTTCCCCGCCGCCGTGACCGTCACCAGAGATGTGACATCTCTTCTTAATCCATCTTCTCTGATAATCGGAAAATCATTATCTCCTTCAAAGTTCAGCTCAATCAAATCCTCCGTTTCATTTTCCCGCAAAACCCTAACTCCAATTCGATACTCTTCATCTCCCGCCGATCACTCACCCGCCACCGCCGTGGAAGCGATCACGAATCGATCCAAAACCTCCTTGAAATCTCGTCTCCGTGGAGGAGAAACTCTCTACGGTCTCTTTTTACTCTCCTTCTCGCCGACATTAGCCGAGATCGCTGCTCACGCCGGTTACGATTACGTCGTCGTTGATATGGAACATGGTCCCGGAGGTATACCGGAAGCTTTGGATTGTATTCGAGCTCTTAACGCCGCCGGAACATCAGCCATTCTCCGATTACCGGAAAACTCACCAACCTGGGCTAAAAAAGCTCTAGATCTAGGTCCACAAGGAATCATGTTCCCAATGATCGAATCTCGTAAAGACGCTACCAAAGCGGTGTCGTATTGCCGGTTTCCTCCCGACGGTATCCGTGGATCGGCGCACACGGTGGTGAGAGCTTCTAACTACGGAATCGATGAAGGGTATTTAAGTAATTACGCAGAGGAGATTCTGATTATGTGCCAGGTGGAATCAGGTGAAGGAGTGAAGAAAGCTGATGAAATCGCAGCCGTTGATGGTGTTGACTGTGTGCAAATGGGACCGTTGGATCTTAGTGCGAGTTTAGGGTATTTGTGGGATCCTGGACATAAGAAAGTGAGAGAGATGATGAAGAAGGCTGAGAAATCTGTGCTGACCACTGATCCGGCGAAAGGCGGGGCTTACTTGTCGGGTTTCGCGATGCCGCACGACGGAGCTGGTGAGATTCGGGGACGTGGTTACCATATGGTCGCCGGAGCTGTTGATGTTGGATTGTTTAGGAATGCTGCTGTTGAAGATGTGAGGAGATTCAAGATGGGTTTGGTCAATGAATCGGACAGTGAGGATTCGTCGGAACATGATAAAGATGTTGATGATGAGAAGTACTGGAGCGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HpcH_HpaI

Protein Analysis

358

Amino Acids

38.4

Weight (kDa)

5.7

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HpcH_HpaI PF03328 91 - 322 1.2e-62 HpcH/HpaI aldolase/citrate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10750
fragaria_vesca FvH4_5g11021 FvH4_5g11021
malus_domestica MD06G1117400.v1.1
prunus_persica Prupe.5G128500_v2.0.a1
pyrus_communis pycom06g10930
rosa_chinensis RchiOBHm_Chr7g0186781
rosa_laevigata RLG00000004816
rosa_multiflora Rmu_co8215596.1_g000001 Rmu_sc0009734.1_g000001 Rmu_sc0040245.1_g000001 Rmu_ssc0000024.1_g000045
rosa_roxburghii Rroxscaffold_3G00267830
rosa_rugosa Rorug06G0476600
rosa_samantha Rh7AG063900 Rh7BG080400 Rh7CG083600 Rh7DG084500
rosa_wichuraiana Rw7G007250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 373
AccII CGCG 1 cut(s) 878
AclWI GGATC 6 cut(s) 219, 583, 756, 773, 786, 836
AcoI YGGCCR 1 cut(s) 3
AdeI CACNNNGTG 1 cut(s) 589
AfaI GTAC 1 cut(s) 1064
AfiI CCNNNNNNNGG 5 cut(s) 439, 563, 577, 851, 890
AgsI TTSAA 5 cut(s) 15, 105, 241, 974, 991
AjiI CACGTC 1 cut(s) 914
AjnI CCWGG 3 cut(s) 455, 663, 781
AleI CACNNNNGTG 2 cut(s) 203, 590
AluBI AGCT 8 cut(s) 114, 383, 401, 470, 599, 695, 896, 938
AluI AGCT 8 cut(s) 114, 383, 401, 470, 599, 695, 896, 938
Alw21I GWGCWC 1 cut(s) 403
Alw26I GTCTC 2 cut(s) 254, 281
AlwI GGATC 6 cut(s) 219, 583, 756, 773, 786, 836
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 3 cut(s) 317, 707, 965
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 3 cut(s) 362, 482, 740
AsuC2I CCSGG 1 cut(s) 366
AsuHPI GGTGA 6 cut(s) 31, 183, 441, 604, 689, 911
AvaII GGWCC 3 cut(s) 362, 482, 740
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 778
BanII GRGCYC 1 cut(s) 403
Bbv12I GWGCWC 1 cut(s) 403
BbvI GCAGC 3 cut(s) 304, 719, 952
BccI CCATC 3 cut(s) 76, 710, 988
BceAI ACGGC 3 cut(s) 14, 188, 695
BcgI CGANNNNNNTGC 2 cut(s) 299, 333
BciT130I CCWGG 3 cut(s) 457, 665, 783
BciVI GTATCC 1 cut(s) 578
BcnI CCSGG 1 cut(s) 366
BcoDI GTCTC 2 cut(s) 254, 281
BfaI CTAG 2 cut(s) 473, 479
BfuI GTATCC 1 cut(s) 578
BglII AGATCT 1 cut(s) 475
BisI GCNGC 6 cut(s) 27, 318, 411, 708, 884, 966
BlsI GCNGC 6 cut(s) 28, 319, 412, 709, 885, 967
BmcAI AGTACT 1 cut(s) 1064
Bme1390I CCNGG 4 cut(s) 366, 457, 665, 783
Bme18I GGWCC 3 cut(s) 362, 482, 740
BmgBI CACGTC 1 cut(s) 914
BmgT120I GGNCC 3 cut(s) 362, 482, 740
BmiI GGNNCC 3 cut(s) 364, 741, 780
BmrFI CCNGG 4 cut(s) 366, 457, 665, 783
BmsI GCATC 1 cut(s) 870
BplI GAGNNNNNCTC 2 cut(s) 252, 284
BpuMI CCSGG 1 cut(s) 366
Bsa29I ATCGAT 2 cut(s) 223, 615
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 4 cut(s) 204, 253, 456, 571
BsaWI WCCGGW 2 cut(s) 376, 439
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bsc4I CCNNNNNNNGG 5 cut(s) 439, 563, 577, 851, 890
Bse118I RCCGGY 2 cut(s) 326, 549
Bse1I ACTGG 1 cut(s) 1070
BseBI CCWGG 3 cut(s) 457, 665, 783
BseCI ATCGAT 2 cut(s) 223, 615
BseDI CCNNGG 4 cut(s) 204, 253, 456, 571
BseLI CCNNNNNNNGG 5 cut(s) 439, 563, 577, 851, 890
BseMII CTCAG 1 cut(s) 810
BseNI ACTGG 1 cut(s) 1070
BseRI GAGGAG 3 cut(s) 273, 659, 997
BseXI GCAGC 3 cut(s) 304, 719, 952
Bsh1236I CGCG 1 cut(s) 878
BshFI GGCC 1 cut(s) 5
BshVI ATCGAT 2 cut(s) 223, 615
BsiHKAI GWGCWC 1 cut(s) 403
BsiSI CCGG 8 cut(s) 327, 366, 377, 414, 440, 550, 845, 933
BslFI GGGAC 3 cut(s) 348, 753, 924
BslI CCNNNNNNNGG 5 cut(s) 439, 563, 577, 851, 890
BsmAI GTCTC 2 cut(s) 254, 281
BsmBI CGTCTC 1 cut(s) 254
BsmFI GGGAC 3 cut(s) 348, 753, 924
BsmI GAATGC 1 cut(s) 967
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 281
Bsp1286I GDGCHC 1 cut(s) 403
Bsp68I TCGCGA 1 cut(s) 878
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 811
BspDI ATCGAT 2 cut(s) 223, 615
BspFNI CGCG 1 cut(s) 878
BspLI GGNNCC 3 cut(s) 364, 741, 780
BspPI GGATC 6 cut(s) 219, 583, 756, 773, 786, 836
BspTNI GGTCTC 1 cut(s) 281
BsrFI RCCGGY 2 cut(s) 326, 549
BsrI ACTGG 1 cut(s) 1070
BssAI RCCGGY 2 cut(s) 326, 549
BssECI CCNNGG 4 cut(s) 204, 253, 456, 571
BssNAI GTATAC 1 cut(s) 374
Bst1107I GTATAC 1 cut(s) 374
Bst2UI CCWGG 3 cut(s) 457, 665, 783
Bst4CI ACNGT 7 cut(s) 37, 275, 566, 589, 728, 744, 1019
Bst6I CTCTTC 2 cut(s) 63, 175
BstDEI CTNAG 2 cut(s) 752, 819
BstDSI CCRYGG 3 cut(s) 204, 253, 571
BstEII GGTNACC 1 cut(s) 917
BstFNI CGCG 1 cut(s) 878
BstHHI GCGC 1 cut(s) 583
BstMAI GTCTC 2 cut(s) 254, 281
BstMWI GCNNNNNNNGC 6 cut(s) 209, 314, 323, 407, 467, 533
BstNI CCWGG 3 cut(s) 457, 665, 783
BstPI GGTNACC 1 cut(s) 917
BstSCI CCNGG 4 cut(s) 364, 455, 663, 781
BstUI CGCG 1 cut(s) 878
BstV1I GCAGC 3 cut(s) 304, 719, 952
BstX2I RGATCY 3 cut(s) 475, 748, 778
BstXI CCANNNNNNTGG 1 cut(s) 205
BstYI RGATCY 3 cut(s) 475, 748, 778
BstZ17I GTATAC 1 cut(s) 374
Bsu15I ATCGAT 2 cut(s) 223, 615
BsuI GTATCC 1 cut(s) 578
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 2 cut(s) 223, 615
BtgI CCRYGG 3 cut(s) 204, 253, 571
BtgZI GCGATG 1 cut(s) 893
BtrI CACGTC 1 cut(s) 914
BtsIMutI CAGTG 2 cut(s) 837, 1024
BtuMI TCGCGA 1 cut(s) 878
CfoI GCGC 1 cut(s) 583
Cfr10I RCCGGY 2 cut(s) 326, 549
Cfr13I GGNCC 3 cut(s) 362, 482, 740
ClaI ATCGAT 2 cut(s) 223, 615
CseI GACGC 1 cut(s) 533
Csp6I GTAC 1 cut(s) 1063
CviAII CATG 3 cut(s) 359, 496, 1037
CviQI GTAC 1 cut(s) 1063
DdeI CTNAG 2 cut(s) 752, 819
DraIII CACNNNGTG 1 cut(s) 589
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 63, 175
EarI CTCTTC 2 cut(s) 63, 175
Ecl136II GAGCTC 1 cut(s) 401
Eco24I GRGCYC 1 cut(s) 403
Eco31I GGTCTC 1 cut(s) 281
Eco47I GGWCC 3 cut(s) 362, 482, 740
Eco53kI GAGCTC 1 cut(s) 401
Eco91I GGTNACC 1 cut(s) 917
EcoICRI GAGCTC 1 cut(s) 401
EcoO65I GGTNACC 1 cut(s) 917
EcoRII CCWGG 3 cut(s) 455, 663, 781
EcoT38I GRGCYC 1 cut(s) 403
Esp3I CGTCTC 1 cut(s) 254
FaeI CATG 3 cut(s) 362, 499, 1040
FaiI YATR 9 cut(s) 353, 360, 374, 497, 659, 789, 924, 926, 1038
FaqI GGGAC 3 cut(s) 348, 753, 924
FatI CATG 3 cut(s) 358, 495, 1036
FauI CCCGC 5 cut(s) 31, 149, 187, 202, 848
FauNDI CATATG 1 cut(s) 924
FblI GTMKAC 1 cut(s) 373
Fnu4HI GCNGC 6 cut(s) 27, 318, 411, 708, 884, 966
FriOI GRGCYC 1 cut(s) 403
Fsp4HI GCNGC 6 cut(s) 27, 318, 411, 708, 884, 966
FspBI CTAG 2 cut(s) 473, 479
GlaI GCGC 1 cut(s) 582
GluI GCNGC 6 cut(s) 27, 318, 411, 708, 884, 966
HaeIII GGCC 1 cut(s) 5
HapII CCGG 8 cut(s) 327, 366, 377, 414, 440, 550, 845, 933
HgaI GACGC 1 cut(s) 533
HhaI GCGC 1 cut(s) 583
Hin1II CATG 3 cut(s) 362, 499, 1040
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HincII GTYRAC 1 cut(s) 724
HindII GTYRAC 1 cut(s) 724
HindIII AAGCTT 1 cut(s) 381
HpaII CCGG 8 cut(s) 327, 366, 377, 414, 440, 550, 845, 933
HphI GGTGA 6 cut(s) 31, 183, 441, 604, 689, 911
Hpy166II GTNNAC 3 cut(s) 374, 485, 724
Hpy188I TCNGA 6 cut(s) 78, 86, 434, 654, 1015, 1033
Hpy188III TCNNGA 5 cut(s) 217, 473, 560, 877, 991
Hpy8I GTNNAC 3 cut(s) 374, 485, 724
Hpy99I CGWCG 5 cut(s) 344, 347, 566, 893, 1033
HpyAV CCTTC 5 cut(s) 111, 301, 614, 674, 808
HpyCH4III ACNGT 7 cut(s) 37, 275, 566, 589, 728, 744, 1019
HpyCH4IV ACGT 2 cut(s) 339, 913
HpyCH4V TGCA 1 cut(s) 733
HpyF10VI GCNNNNNNNGC 6 cut(s) 209, 314, 323, 407, 467, 533
HpyF3I CTNAG 2 cut(s) 752, 819
HpySE526I ACGT 2 cut(s) 339, 913
Hsp92II CATG 3 cut(s) 362, 499, 1040
HspAI GCGC 1 cut(s) 581
LmnI GCTCC 3 cut(s) 893, 935, 1068
Lsp1109I GCAGC 3 cut(s) 304, 719, 952
LweI GCATC 1 cut(s) 870
MaeI CTAG 2 cut(s) 473, 479
MaeII ACGT 2 cut(s) 339, 913
MaeIII GTNAC 5 cut(s) 31, 37, 49, 329, 917
MboII GAAGA 7 cut(s) 10, 50, 63, 162, 700, 823, 986
MflI RGATCY 3 cut(s) 475, 748, 778
MhlI GDGCHC 1 cut(s) 403
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 159, 634
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 726, 928, 1011
MnlI CCTC 8 cut(s) 136, 244, 251, 362, 567, 637, 975, 1015
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 63, 405, 629
MslI CAYNNNNRTG 2 cut(s) 203, 590
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 8 cut(s) 327, 366, 377, 414, 440, 550, 845, 933
MspR9I CCNGG 4 cut(s) 366, 457, 665, 783
Mva1269I GAATGC 1 cut(s) 967
MvaI CCWGG 3 cut(s) 457, 665, 783
MvnI CGCG 1 cut(s) 878
MwoI GCNNNNNNNGC 6 cut(s) 209, 314, 323, 407, 467, 533
NciI CCSGG 1 cut(s) 366
NdeI CATATG 1 cut(s) 924
NlaIII CATG 3 cut(s) 362, 499, 1040
NlaIV GGNNCC 3 cut(s) 364, 741, 780
NmeAIII GCCGAG 1 cut(s) 334
NmuCI GTSAC 3 cut(s) 31, 37, 49
NruI TCGCGA 1 cut(s) 878
OliI CACNNNNGTG 2 cut(s) 203, 590
PcsI WCGNNNNNNNCGW 3 cut(s) 330, 339, 875
PctI GAATGC 1 cut(s) 967
PfoI TCCNGGA 2 cut(s) 364, 781
PkrI GCNGC 6 cut(s) 28, 319, 412, 709, 885, 967
Psp124BI GAGCTC 1 cut(s) 403
Psp6I CCWGG 3 cut(s) 455, 663, 781
PspEI GGTNACC 1 cut(s) 917
PspGI CCWGG 3 cut(s) 455, 663, 781
PspN4I GGNNCC 3 cut(s) 364, 741, 780
PspPI GGNCC 3 cut(s) 362, 482, 740
PsuI RGATCY 3 cut(s) 475, 748, 778
RruI TCGCGA 1 cut(s) 878
RsaI GTAC 1 cut(s) 1064
RsaNI GTAC 1 cut(s) 1063
RseI CAYNNNNRTG 2 cut(s) 203, 590
SacI GAGCTC 1 cut(s) 403
SaqAI TTAA 3 cut(s) 63, 405, 629
SatI GCNGC 6 cut(s) 27, 318, 411, 708, 884, 966
Sau96I GGNCC 3 cut(s) 362, 482, 740
ScaI AGTACT 1 cut(s) 1064
ScrFI CCNGG 4 cut(s) 366, 457, 665, 783
SduI GDGCHC 1 cut(s) 403
SfaNI GCATC 1 cut(s) 870
SinI GGWCC 3 cut(s) 362, 482, 740
SmiMI CAYNNNNRTG 2 cut(s) 203, 590
Sse9I AATT 2 cut(s) 159, 634
SspMI CTAG 2 cut(s) 473, 479
SstI GAGCTC 1 cut(s) 403
StyD4I CCNGG 4 cut(s) 364, 455, 663, 781
TaaI ACNGT 7 cut(s) 37, 275, 566, 589, 728, 744, 1019
TaiI ACGT 2 cut(s) 342, 916
TaqI TCGA 5 cut(s) 163, 223, 397, 510, 615
TasI AATT 2 cut(s) 159, 634
TatI WGTACW 1 cut(s) 1062
TauI GCSGC 3 cut(s) 29, 413, 886
Tru1I TTAA 3 cut(s) 63, 405, 629
Tru9I TTAA 3 cut(s) 63, 405, 629
TscAI CASTG 2 cut(s) 844, 1024
TseFI GTSAC 3 cut(s) 31, 37, 49
TseI GCWGC 3 cut(s) 317, 707, 965
Tsp45I GTSAC 3 cut(s) 31, 37, 49
TspDTI ATGAA 6 cut(s) 123, 162, 633, 714, 824, 1023
TspGWI ACGGA 5 cut(s) 118, 242, 560, 624, 906
TspRI CASTG 2 cut(s) 844, 1024
VpaK11BI GGWCC 3 cut(s) 362, 482, 740
XbaI TCTAGA 1 cut(s) 472
XmiI GTMKAC 1 cut(s) 373
XspI CTAG 2 cut(s) 473, 479
ZrmI AGTACT 1 cut(s) 1064
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.