Rw7G007250

HpcH/HpaI aldolase/citrate lyase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012383
Physical Location & Seq
Reverse (-)
6273517 .. 6274888
1372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G007250.1

Sequence Viewer

Length: 1071 bp
ATGGCCACACTGACTCACGCCGCCGCCGCCATCGCCATCGCCATCACCCCCTCATCACCTCTCGCAAAACCCATCTCCTCCAGTCCCACCACCACCAATCCCCTATCCCATCTCTCTTTCCCTTTCTCCACATCCAAAACCCTAAACCTCACCGCCAAAACCACCTCAATCAAATCCTCCTCCTCCTCCTCCGATCTCTCCACCACCACCGTAACGACGACCTCCATCTCCTCCTCCCCGCAAACCCTCAAGTCCCGCCTCCACAATGGCGACACCCTCTACGGCCTCTTCCTCCTCAGCTTCTCCCCCACCCTCGCCGAGATCGCCGGCCTCTCCGGCTACGACTTCGTCGTCGTCGACATGGAGCACGGCCCCGGCGGAATCTCCGAAGCCCTCTCCTGCCTCCGCGCCCTCGCCGCCACCCAAACCCCGGCGATCCTCCGGCTGCCGGAATCCTCCCCGACCTGGGCCAAGAAAGCCCTCGATCTCGGCCCGCAGGGGATCATGTTCCCGATGATCGACAGCTCCAAGGAGGCCGAGAAAGCCGTCTCCTACTGCCGCTTCCCTCCCGCCGGCGTCCGCGGCTCCGCACACACCGTCGTCCGCGCCTCCAGCTACGGCATCGACGAGGGGTATTTGAGTAATTACGAGGACCAATTGCTGATCATGTGCCAGGTGGAGACCGAGGAGGGCGTGAAGAGAGCGGAGGAGATCGCAGCCGTCGAAGGCGTTGACTGCGTGCAGATGGGGCCGTTGGATCTGAGCGCCAGCCTGGGGTACCTGTGGGACCCGGGGAACAAGAAGGTCCGGAGCGCGTTGAAGGTTGCCGAGAAGGCCGTGCTGGGCACCGACCCCCTTGTGGGTGGGGCCTACTTGGCTGGGTTCGCTATGCCCCACGATGGGCCCCAGGACCTGAGGACACGTGGCTACCACATGGTGTCCGGTTCCGTCGACGTCGGGCTGTTTAGGAGCGCAGCGGTGGAGGACGTGAGGAGGTTTCAGGCGAGCTTGATCAACGGCTCTGATGAGGAGCGTGGGGATGATGCAGATGAGAAGTACTGGAGCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HpcH_HpaI

Protein Analysis

356

Amino Acids

37.67

Weight (kDa)

5.16

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HpcH_HpaI PF03328 94 - 324 2.3e-54 HpcH/HpaI aldolase/citrate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10750
fragaria_vesca FvH4_5g11021 FvH4_5g11021
malus_domestica MD06G1117400.v1.1
prunus_persica Prupe.5G128500_v2.0.a1
pyrus_communis pycom06g10930
rosa_chinensis RchiOBHm_Chr7g0186781
rosa_laevigata RLG00000004816
rosa_multiflora Rmu_co8215596.1_g000001 Rmu_sc0009734.1_g000001 Rmu_sc0040245.1_g000001 Rmu_ssc0000024.1_g000045
rosa_roxburghii Rroxscaffold_3G00267830
rosa_rugosa Rorug06G0476600
rosa_samantha Rh7AG063900 Rh7BG080400 Rh7CG083600 Rh7DG084500
rosa_wichuraiana Rw7G007250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 350
AatII GACGTC 1 cut(s) 957
Acc65I GGTACC 1 cut(s) 777
AccB1I GGYRCC 2 cut(s) 777, 845
AccBSI CCGCTC 1 cut(s) 704
AccI GTMKAC 2 cut(s) 357, 951
AccII CGCG 4 cut(s) 408, 582, 606, 815
AccIII TCCGGA 1 cut(s) 807
AclWI GGATC 3 cut(s) 430, 509, 765
AcoI YGGCCR 1 cut(s) 3
AcvI CACGTG 1 cut(s) 923
AcyI GRCGYC 2 cut(s) 576, 954
AdeI CACNNNGTG 1 cut(s) 937
AfaI GTAC 2 cut(s) 779, 1058
AflIII ACRYGT 1 cut(s) 920
AgsI TTSAA 1 cut(s) 820
AjiI CACGTC 1 cut(s) 988
AjnI CCWGG 4 cut(s) 464, 672, 771, 906
AluBI AGCT 4 cut(s) 300, 525, 615, 1008
AluI AGCT 4 cut(s) 300, 525, 615, 1008
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 2 cut(s) 553, 674
AlwI GGATC 3 cut(s) 430, 509, 765
AlwNI CAGNNNCTG 1 cut(s) 913
Ama87I CYCGRG 1 cut(s) 790
Aor13HI TCCGGA 1 cut(s) 807
ApaI GGGCCC 1 cut(s) 906
ApeKI GCWGC 3 cut(s) 445, 716, 974
Asp718I GGTACC 1 cut(s) 777
AspLEI GCGC 5 cut(s) 410, 608, 767, 815, 974
AsuC2I CCSGG 4 cut(s) 375, 431, 791, 792
AsuHPI GGTGA 3 cut(s) 37, 48, 142
AvaI CYCGRG 1 cut(s) 790
AvaII GGWCC 4 cut(s) 652, 787, 805, 910
AxyI CCTNAGG 1 cut(s) 914
BaeGI GKGCMC 2 cut(s) 848, 906
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 777, 845
BanII GRGCYC 1 cut(s) 906
BbrPI CACGTG 1 cut(s) 923
Bbv12I GWGCWC 1 cut(s) 369
BbvCI CCTCAGC 1 cut(s) 296
BbvI GCAGC 3 cut(s) 432, 728, 986
BccI CCATC 8 cut(s) 38, 44, 50, 80, 117, 233, 739, 893
BceAI ACGGC 8 cut(s) 298, 385, 530, 634, 704, 736, 821, 1033
BciT130I CCWGG 4 cut(s) 466, 674, 773, 908
BclI TGATCA 2 cut(s) 663, 1011
BcnI CCSGG 4 cut(s) 375, 431, 791, 792
BcoDI GTCTC 2 cut(s) 553, 674
BfoI RGCGCY 1 cut(s) 768
BglI GCCNNNNNGGC 3 cut(s) 336, 833, 875
BisI GCNGC 9 cut(s) 21, 24, 27, 417, 446, 559, 583, 717, 975
BlsI GCNGC 9 cut(s) 22, 25, 28, 418, 447, 560, 584, 718, 976
BmcAI AGTACT 1 cut(s) 1058
Bme1390I CCNGG 8 cut(s) 375, 431, 466, 674, 773, 791, 792, 908
Bme18I GGWCC 4 cut(s) 652, 787, 805, 910
BmeT110I CYCGRG 1 cut(s) 790
BmgBI CACGTC 1 cut(s) 988
BmrFI CCNGG 8 cut(s) 375, 431, 466, 674, 773, 791, 792, 908
BmsI GCATC 2 cut(s) 630, 1033
BpmI CTGGAG 2 cut(s) 64, 595
Bpu10I CCTNAGC 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 233
BpuMI CCSGG 4 cut(s) 375, 431, 791, 792
BsaAI YACGTR 1 cut(s) 923
BsaHI GRCGYC 2 cut(s) 576, 954
BsaI GGTCTC 1 cut(s) 674
BsaWI WCCGGW 2 cut(s) 807, 941
Bse118I RCCGGY 2 cut(s) 326, 572
Bse1I ACTGG 2 cut(s) 81, 1064
Bse21I CCTNAGG 1 cut(s) 914
BseAI TCCGGA 1 cut(s) 807
BseBI CCWGG 4 cut(s) 466, 674, 773, 908
BseGI GGATG 2 cut(s) 131, 1045
BseMII CTCAG 3 cut(s) 310, 752, 905
BseNI ACTGG 2 cut(s) 81, 1064
BseSI GKGCMC 2 cut(s) 848, 906
BseXI GCAGC 3 cut(s) 432, 728, 986
BseYI CCCAGC 2 cut(s) 841, 878
BsgI GTGCAG 1 cut(s) 761
Bsh1236I CGCG 4 cut(s) 408, 582, 606, 815
BshNI GGYRCC 2 cut(s) 777, 845
BsiHKAI GWGCWC 1 cut(s) 369
BsiHKCI CYCGRG 1 cut(s) 790
BslFI GGGAC 3 cut(s) 69, 238, 800
BsmAI GTCTC 2 cut(s) 553, 674
BsmBI CGTCTC 1 cut(s) 553
BsmFI GGGAC 3 cut(s) 69, 238, 800
Bso31I GGTCTC 1 cut(s) 674
BsoBI CYCGRG 1 cut(s) 790
Bsp120I GGGCCC 1 cut(s) 902
Bsp1286I GDGCHC 3 cut(s) 369, 848, 906
Bsp13I TCCGGA 1 cut(s) 807
BspCNI CTCAG 3 cut(s) 309, 753, 906
BspEI TCCGGA 1 cut(s) 807
BspFNI CGCG 4 cut(s) 408, 582, 606, 815
BspPI GGATC 3 cut(s) 430, 509, 765
BspT107I GGYRCC 2 cut(s) 777, 845
BspTNI GGTCTC 1 cut(s) 674
BsrBI CCGCTC 1 cut(s) 704
BsrFI RCCGGY 2 cut(s) 326, 572
BsrI ACTGG 2 cut(s) 81, 1064
BssAI RCCGGY 2 cut(s) 326, 572
BssNI GRCGYC 2 cut(s) 576, 954
BssT1I CCWWGG 1 cut(s) 528
Bst2UI CCWGG 4 cut(s) 466, 674, 773, 908
Bst4CI ACNGT 2 cut(s) 211, 598
Bst6I CTCTTC 2 cut(s) 293, 692
BstACI GRCGYC 2 cut(s) 576, 954
BstBAI YACGTR 1 cut(s) 923
BstC8I GCNNGC 6 cut(s) 328, 494, 574, 740, 769, 1006
BstDEI CTNAG 3 cut(s) 296, 761, 914
BstDSI CCRYGG 1 cut(s) 580
BstF5I GGATG 2 cut(s) 131, 1045
BstFNI CGCG 4 cut(s) 408, 582, 606, 815
BstH2I RGCGCY 1 cut(s) 768
BstHHI GCGC 5 cut(s) 410, 608, 767, 815, 974
BstMAI GTCTC 2 cut(s) 553, 674
BstNI CCWGG 4 cut(s) 466, 674, 773, 908
BstSCI CCNGG 8 cut(s) 373, 429, 464, 672, 771, 789, 790, 906
BstSLI GKGCMC 2 cut(s) 848, 906
BstUI CGCG 4 cut(s) 408, 582, 606, 815
BstV1I GCAGC 3 cut(s) 432, 728, 986
BstX2I RGATCY 1 cut(s) 757
BstYI RGATCY 1 cut(s) 757
Bsu36I CCTNAGG 1 cut(s) 914
BtgI CCRYGG 1 cut(s) 580
BtgZI GCGATG 2 cut(s) 16, 22
BtrI CACGTC 1 cut(s) 988
BtsCI GGATG 2 cut(s) 131, 1045
BtsIMutI CAGTG 1 cut(s) 8
Cac8I GCNNGC 6 cut(s) 328, 494, 574, 740, 769, 1006
CaiI CAGNNNCTG 1 cut(s) 913
CfoI GCGC 5 cut(s) 410, 608, 767, 815, 974
Cfr10I RCCGGY 2 cut(s) 326, 572
Cfr42I CCGCGG 1 cut(s) 583
Cfr9I CCCGGG 1 cut(s) 790
CseI GACGC 1 cut(s) 565
Csp6I GTAC 2 cut(s) 778, 1057
CviAII CATG 4 cut(s) 361, 505, 667, 934
CviQI GTAC 2 cut(s) 778, 1057
DdeI CTNAG 3 cut(s) 296, 761, 914
DraIII CACNNNGTG 1 cut(s) 937
DrdI GACNNNNNNGTC 1 cut(s) 350
DseDI GACNNNNNNGTC 1 cut(s) 350
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 293, 692
EarI CTCTTC 2 cut(s) 293, 692
EciI GGCGGA 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 528
Eco24I GRGCYC 1 cut(s) 906
Eco31I GGTCTC 1 cut(s) 674
Eco47I GGWCC 4 cut(s) 652, 787, 805, 910
Eco72I CACGTG 1 cut(s) 923
Eco81I CCTNAGG 1 cut(s) 914
Eco88I CYCGRG 1 cut(s) 790
EcoO109I RGGNCCY 4 cut(s) 787, 867, 903, 910
EcoRII CCWGG 4 cut(s) 464, 672, 771, 906
EcoT14I CCWWGG 1 cut(s) 528
EcoT38I GRGCYC 1 cut(s) 906
ErhI CCWWGG 1 cut(s) 528
Esp3I CGTCTC 1 cut(s) 553
FaeI CATG 4 cut(s) 364, 508, 670, 937
FaiI YATR 5 cut(s) 362, 506, 668, 890, 935
FaqI GGGAC 3 cut(s) 69, 238, 800
FatI CATG 4 cut(s) 360, 504, 666, 933
FauI CCCGC 4 cut(s) 246, 263, 501, 577
FbaI TGATCA 2 cut(s) 663, 1011
FblI GTMKAC 2 cut(s) 357, 951
Fnu4HI GCNGC 9 cut(s) 21, 24, 27, 417, 446, 559, 583, 717, 975
FokI GGATG 2 cut(s) 118, 1052
FriOI GRGCYC 1 cut(s) 906
Fsp4HI GCNGC 9 cut(s) 21, 24, 27, 417, 446, 559, 583, 717, 975
GlaI GCGC 5 cut(s) 409, 607, 766, 814, 973
GluI GCNGC 9 cut(s) 21, 24, 27, 417, 446, 559, 583, 717, 975
GsaI CCCAGC 2 cut(s) 845, 882
GsuI CTGGAG 2 cut(s) 64, 595
HaeII RGCGCY 1 cut(s) 768
HgaI GACGC 1 cut(s) 565
HhaI GCGC 5 cut(s) 410, 608, 767, 815, 974
Hin1I GRCGYC 2 cut(s) 576, 954
Hin1II CATG 4 cut(s) 364, 508, 670, 937
Hin6I GCGC 5 cut(s) 408, 606, 765, 813, 972
HinP1I GCGC 5 cut(s) 408, 606, 765, 813, 972
HincII GTYRAC 3 cut(s) 358, 733, 952
HindII GTYRAC 3 cut(s) 358, 733, 952
HinfI GANTC 3 cut(s) 13, 381, 452
HphI GGTGA 3 cut(s) 37, 48, 142
Hpy166II GTNNAC 3 cut(s) 358, 733, 952
Hpy188I TCNGA 4 cut(s) 193, 388, 762, 1024
Hpy188III TCNNGA 2 cut(s) 511, 808
Hpy8I GTNNAC 3 cut(s) 358, 733, 952
HpyAV CCTTC 4 cut(s) 719, 796, 814, 826
HpyCH4III ACNGT 2 cut(s) 211, 598
HpyCH4IV ACGT 3 cut(s) 922, 954, 987
HpyCH4V TGCA 2 cut(s) 742, 1046
HpyF3I CTNAG 3 cut(s) 296, 761, 914
HpySE526I ACGT 3 cut(s) 922, 954, 987
Hsp92I GRCGYC 2 cut(s) 576, 954
Hsp92II CATG 4 cut(s) 364, 508, 670, 937
HspAI GCGC 5 cut(s) 408, 606, 765, 813, 972
KflI GGGWCCC 1 cut(s) 787
Kpn2I TCCGGA 1 cut(s) 807
KpnI GGTACC 1 cut(s) 781
KroI GCCGGC 2 cut(s) 326, 572
KroNI GCCGGC 2 cut(s) 328, 574
Ksp22I TGATCA 2 cut(s) 663, 1011
KspI CCGCGG 1 cut(s) 583
LmnI GCTCC 7 cut(s) 364, 530, 590, 810, 969, 1030, 1062
Lsp1109I GCAGC 3 cut(s) 432, 728, 986
LweI GCATC 2 cut(s) 630, 1033
MaeII ACGT 3 cut(s) 922, 954, 987
MaeIII GTNAC 1 cut(s) 211
MbiI CCGCTC 1 cut(s) 704
MboII GAAGA 2 cut(s) 280, 709
MfeI CAATTG 1 cut(s) 656
MflI RGATCY 1 cut(s) 757
MhlI GDGCHC 3 cut(s) 369, 848, 906
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 643, 656
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 735
Mox20I TGGCCA 1 cut(s) 5
MreI CGCCGGCG 1 cut(s) 572
MroI TCCGGA 1 cut(s) 807
MroNI GCCGGC 2 cut(s) 326, 572
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 582, 977
MspR9I CCNGG 8 cut(s) 375, 431, 466, 674, 773, 791, 792, 908
MunI CAATTG 1 cut(s) 656
MvaI CCWGG 4 cut(s) 466, 674, 773, 908
MvnI CGCG 4 cut(s) 408, 582, 606, 815
NaeI GCCGGC 2 cut(s) 328, 574
NciI CCSGG 4 cut(s) 375, 431, 791, 792
NgoMIV GCCGGC 2 cut(s) 326, 572
NlaIII CATG 4 cut(s) 364, 508, 670, 937
NmeAIII GCCGAG 4 cut(s) 343, 468, 562, 853
PcsI WCGNNNNNNNCGW 4 cut(s) 348, 354, 624, 720
PdiI GCCGGC 2 cut(s) 328, 574
PfeI GAWTC 2 cut(s) 381, 452
PflFI GACNNNGTC 1 cut(s) 347
PkrI GCNGC 9 cut(s) 22, 25, 28, 418, 447, 560, 584, 718, 976
PleI GAGTC 1 cut(s) 7
PmaCI CACGTG 1 cut(s) 923
PmlI CACGTG 1 cut(s) 923
PpsI GAGTC 1 cut(s) 7
Ppu21I YACGTR 1 cut(s) 923
PpuMI RGGWCCY 2 cut(s) 787, 910
Psp5II RGGWCCY 2 cut(s) 787, 910
Psp6I CCWGG 4 cut(s) 464, 672, 771, 906
PspCI CACGTG 1 cut(s) 923
PspFI CCCAGC 2 cut(s) 841, 878
PspGI CCWGG 4 cut(s) 464, 672, 771, 906
PspOMI GGGCCC 1 cut(s) 902
PspPPI RGGWCCY 2 cut(s) 787, 910
PstNI CAGNNNCTG 1 cut(s) 913
PsuI RGATCY 1 cut(s) 757
PsyI GACNNNGTC 1 cut(s) 347
RsaI GTAC 2 cut(s) 779, 1058
RsaNI GTAC 2 cut(s) 778, 1057
SacII CCGCGG 1 cut(s) 583
SalI GTCGAC 2 cut(s) 356, 950
SatI GCNGC 9 cut(s) 21, 24, 27, 417, 446, 559, 583, 717, 975
ScaI AGTACT 1 cut(s) 1058
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 8 cut(s) 375, 431, 466, 674, 773, 791, 792, 908
SduI GDGCHC 3 cut(s) 369, 848, 906
SfaNI GCATC 2 cut(s) 630, 1033
Sfr303I CCGCGG 1 cut(s) 583
SgrAI CRCCGGYG 1 cut(s) 572
SgrBI CCGCGG 1 cut(s) 583
SgrDI CGTCGACG 1 cut(s) 950
SinI GGWCC 4 cut(s) 652, 787, 805, 910
SmaI CCCGGG 1 cut(s) 792
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 2 cut(s) 643, 656
StyD4I CCNGG 8 cut(s) 373, 429, 464, 672, 771, 789, 790, 906
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 2 cut(s) 211, 598
TaiI ACGT 3 cut(s) 925, 957, 990
TaqI TCGA 6 cut(s) 357, 483, 519, 624, 723, 951
TaqII GACCGA 1 cut(s) 698
TasI AATT 2 cut(s) 643, 656
TatI WGTACW 1 cut(s) 1056
TauI GCSGC 6 cut(s) 23, 26, 29, 419, 561, 585
TfiI GAWTC 2 cut(s) 381, 452
TscAI CASTG 1 cut(s) 15
TseI GCWGC 3 cut(s) 445, 716, 974
TspGWI ACGGA 1 cut(s) 937
TspMI CCCGGG 1 cut(s) 790
TspRI CASTG 1 cut(s) 15
Tth111I GACNNNGTC 1 cut(s) 347
VpaK11BI GGWCC 4 cut(s) 652, 787, 805, 910
XmaI CCCGGG 1 cut(s) 790
XmiI GTMKAC 2 cut(s) 357, 951
ZraI GACGTC 1 cut(s) 955
ZrmI AGTACT 1 cut(s) 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.