Rh7DG084500

HpcH/HpaI aldolase/citrate lyase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012383
Physical Location & Seq
Reverse (-)
6758772 .. 6759839
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG084500.1

Sequence Viewer

Length: 1068 bp
ATGGCCACACTGACTCACGCCGCCGCCGCCGCCGCCATCGCCATCACCGCCTCATCACCGCTCGCAAAACCCATCTCTTCCAGTCGCACCACCACCAATCCCCTATCCCATCTCTCTTTCCCTTTCTCCCCATCCAAAACCCTAAACCTCACCGCCAAAACCACCTCAATCAAATCCTCCTCCTCCTCCGATCTCTCCACCACCACCGTAACGACGACCTCCATCTCCTCCTCCCCGCAAACCCTCAAGTCCCGCCTCCACAATGGCGACACCCTCTACGGCCTCTTCCTCCTCAGCTTCTCCCCCACCCTCGCCGAGATTGCCGGCCTCTCCGGCTACGACTTTGTCGTCGTCGACATGGAGCACGGCCCCGGCGGAATCTCCGAAGCCCTCTCCTGCCTCCGCGCCCTCGCCGCCACCCAAACCCCGGCGATCCTCCGGCTGCCGGAATCCTCCCCGACCTGGGCCAAGAAAGCCCTCGATCTCGGCCCGCAGGGGATCATGTTCCCGATGATCGACAGCTCCAAGGAGGCCGAGAAAGCCGTCTCCTACTGCCGCTTCCCTCCCGCCGGCGTCCGCGGCTCCGCACACACCGTCGTCCGCGCCTCCAGCTACGGCATCGACGAGGGGTATTTGAGTAATTACGAGGACCAATTGCTGATCATGTGCCAGGTGGAGACCGAGGAGGGCGTGAAGAGAGCGGAGGAGATCGCGGCCGTCGCAAGCGTTGACTGCGTGCAGATGGGGCCGTTGGATCTGAGCGCCAGCCTTGGGTACCTGTGGGACCCGGGGAACAAGAAGGTCCGGAACGCGTTGAAGGTTGCCGAGAAGGCCGTGCTGGGCACCGACCCCCTTGTGGGTGGGGCCTACTTGGCTGGGTTCGCTATGCCCCACGATGGGCCCCAGGACCTGAGGACACGTGGCTACCACATGGTGTCCGGTTCCGTCGACGTCGGGCTGTTTAGGAGCGCAGCGGTGGAGGACGTGAGGAGGTTTCAGGCGAGCTTGATCAACGGCTCTGATGAGGAGCGTGGGGATGATGCAGATGAGAAGTACTGGAGCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HpcH_HpaI

Protein Analysis

355

Amino Acids

37.57

Weight (kDa)

5.34

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HpcH_HpaI PF03328 93 - 323 2.3e-53 HpcH/HpaI aldolase/citrate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10750
fragaria_vesca FvH4_5g11021 FvH4_5g11021
malus_domestica MD06G1117400.v1.1
prunus_persica Prupe.5G128500_v2.0.a1
pyrus_communis pycom06g10930
rosa_chinensis RchiOBHm_Chr7g0186781
rosa_laevigata RLG00000004816
rosa_multiflora Rmu_co8215596.1_g000001 Rmu_sc0009734.1_g000001 Rmu_sc0040245.1_g000001 Rmu_ssc0000024.1_g000045
rosa_roxburghii Rroxscaffold_3G00267830
rosa_rugosa Rorug06G0476600
rosa_samantha Rh7AG063900 Rh7BG080400 Rh7CG083600 Rh7DG084500
rosa_wichuraiana Rw7G007250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 347
AatII GACGTC 1 cut(s) 954
Acc65I GGTACC 1 cut(s) 774
AccB1I GGYRCC 2 cut(s) 774, 842
AccBSI CCGCTC 2 cut(s) 61, 701
AccI GTMKAC 2 cut(s) 354, 948
AccII CGCG 5 cut(s) 405, 579, 603, 713, 812
AccIII TCCGGA 1 cut(s) 804
AclWI GGATC 3 cut(s) 427, 506, 762
AcoI YGGCCR 2 cut(s) 3, 714
AcvI CACGTG 1 cut(s) 920
AcyI GRCGYC 2 cut(s) 573, 951
AdeI CACNNNGTG 1 cut(s) 934
AfaI GTAC 2 cut(s) 776, 1055
AflIII ACRYGT 2 cut(s) 810, 917
AgsI TTSAA 1 cut(s) 817
AjiI CACGTC 1 cut(s) 985
AjnI CCWGG 3 cut(s) 461, 669, 903
AluBI AGCT 4 cut(s) 297, 522, 612, 1005
AluI AGCT 4 cut(s) 297, 522, 612, 1005
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 2 cut(s) 550, 671
AlwI GGATC 3 cut(s) 427, 506, 762
AlwNI CAGNNNCTG 1 cut(s) 910
Ama87I CYCGRG 1 cut(s) 787
Aor13HI TCCGGA 1 cut(s) 804
ApaI GGGCCC 1 cut(s) 903
ApeKI GCWGC 2 cut(s) 442, 971
Asp718I GGTACC 1 cut(s) 774
AspLEI GCGC 4 cut(s) 407, 605, 764, 971
AsuC2I CCSGG 4 cut(s) 372, 428, 788, 789
AsuHPI GGTGA 3 cut(s) 37, 48, 142
AvaI CYCGRG 1 cut(s) 787
AvaII GGWCC 4 cut(s) 649, 784, 802, 907
AxyI CCTNAGG 1 cut(s) 911
BaeGI GKGCMC 2 cut(s) 845, 903
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 774, 842
BanII GRGCYC 1 cut(s) 903
BbrPI CACGTG 1 cut(s) 920
Bbv12I GWGCWC 1 cut(s) 366
BbvCI CCTCAGC 1 cut(s) 293
BbvI GCAGC 2 cut(s) 429, 983
BccI CCATC 8 cut(s) 44, 50, 80, 117, 139, 230, 736, 890
BceAI ACGGC 8 cut(s) 295, 382, 527, 631, 701, 733, 818, 1030
BciT130I CCWGG 3 cut(s) 463, 671, 905
BclI TGATCA 2 cut(s) 660, 1008
BcnI CCSGG 4 cut(s) 372, 428, 788, 789
BcoDI GTCTC 2 cut(s) 550, 671
BfoI RGCGCY 1 cut(s) 765
BglI GCCNNNNNGGC 3 cut(s) 333, 830, 872
BmcAI AGTACT 1 cut(s) 1055
Bme1390I CCNGG 7 cut(s) 372, 428, 463, 671, 788, 789, 905
Bme18I GGWCC 4 cut(s) 649, 784, 802, 907
BmeT110I CYCGRG 1 cut(s) 787
BmgBI CACGTC 1 cut(s) 985
BmrFI CCNGG 7 cut(s) 372, 428, 463, 671, 788, 789, 905
BmsI GCATC 2 cut(s) 627, 1030
BpmI CTGGAG 1 cut(s) 592
Bpu10I CCTNAGC 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 230
BpuMI CCSGG 4 cut(s) 372, 428, 788, 789
BsaAI YACGTR 1 cut(s) 920
BsaHI GRCGYC 2 cut(s) 573, 951
BsaI GGTCTC 1 cut(s) 671
BsaWI WCCGGW 2 cut(s) 804, 938
Bse118I RCCGGY 2 cut(s) 323, 569
Bse1I ACTGG 2 cut(s) 81, 1061
Bse21I CCTNAGG 1 cut(s) 911
BseAI TCCGGA 1 cut(s) 804
BseBI CCWGG 3 cut(s) 463, 671, 905
BseGI GGATG 2 cut(s) 131, 1042
BseMII CTCAG 3 cut(s) 307, 749, 902
BseNI ACTGG 2 cut(s) 81, 1061
BseSI GKGCMC 2 cut(s) 845, 903
BseX3I CGGCCG 1 cut(s) 714
BseXI GCAGC 2 cut(s) 429, 983
BseYI CCCAGC 2 cut(s) 838, 875
BsgI GTGCAG 1 cut(s) 758
Bsh1236I CGCG 5 cut(s) 405, 579, 603, 713, 812
Bsh1285I CGRYCG 1 cut(s) 717
BshNI GGYRCC 2 cut(s) 774, 842
BsiEI CGRYCG 1 cut(s) 717
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 1 cut(s) 787
BslFI GGGAC 2 cut(s) 235, 797
BsmAI GTCTC 2 cut(s) 550, 671
BsmBI CGTCTC 1 cut(s) 550
BsmFI GGGAC 2 cut(s) 235, 797
Bso31I GGTCTC 1 cut(s) 671
BsoBI CYCGRG 1 cut(s) 787
Bsp120I GGGCCC 1 cut(s) 899
Bsp1286I GDGCHC 3 cut(s) 366, 845, 903
Bsp13I TCCGGA 1 cut(s) 804
Bsp143I GATC 9 cut(s) 190, 432, 481, 498, 513, 660, 708, 754, 1008
BspCNI CTCAG 3 cut(s) 306, 750, 903
BspEI TCCGGA 1 cut(s) 804
BspFNI CGCG 5 cut(s) 405, 579, 603, 713, 812
BspPI GGATC 3 cut(s) 427, 506, 762
BspT107I GGYRCC 2 cut(s) 774, 842
BspTNI GGTCTC 1 cut(s) 671
BsrBI CCGCTC 2 cut(s) 61, 701
BsrFI RCCGGY 2 cut(s) 323, 569
BsrI ACTGG 2 cut(s) 81, 1061
BssAI RCCGGY 2 cut(s) 323, 569
BssMI GATC 9 cut(s) 190, 432, 481, 498, 513, 660, 708, 754, 1008
BssNI GRCGYC 2 cut(s) 573, 951
BssT1I CCWWGG 2 cut(s) 525, 769
Bst2UI CCWGG 3 cut(s) 463, 671, 905
Bst4CI ACNGT 2 cut(s) 208, 595
Bst6I CTCTTC 3 cut(s) 82, 290, 689
BstACI GRCGYC 2 cut(s) 573, 951
BstBAI YACGTR 1 cut(s) 920
BstC8I GCNNGC 8 cut(s) 63, 325, 491, 571, 724, 737, 766, 1003
BstDEI CTNAG 3 cut(s) 293, 758, 911
BstDSI CCRYGG 1 cut(s) 577
BstF5I GGATG 2 cut(s) 131, 1042
BstFNI CGCG 5 cut(s) 405, 579, 603, 713, 812
BstH2I RGCGCY 1 cut(s) 765
BstHHI GCGC 4 cut(s) 407, 605, 764, 971
BstKTI GATC 9 cut(s) 193, 435, 484, 501, 516, 663, 711, 757, 1011
BstMAI GTCTC 2 cut(s) 550, 671
BstMBI GATC 9 cut(s) 190, 432, 481, 498, 513, 660, 708, 754, 1008
BstMCI CGRYCG 1 cut(s) 717
BstNI CCWGG 3 cut(s) 463, 671, 905
BstSCI CCNGG 7 cut(s) 370, 426, 461, 669, 786, 787, 903
BstSLI GKGCMC 2 cut(s) 845, 903
BstUI CGCG 5 cut(s) 405, 579, 603, 713, 812
BstV1I GCAGC 2 cut(s) 429, 983
BstX2I RGATCY 1 cut(s) 754
BstYI RGATCY 1 cut(s) 754
BstZI CGGCCG 1 cut(s) 714
Bsu36I CCTNAGG 1 cut(s) 911
BtgI CCRYGG 1 cut(s) 577
BtgZI GCGATG 1 cut(s) 22
BtrI CACGTC 1 cut(s) 985
BtsCI GGATG 2 cut(s) 131, 1042
BtsIMutI CAGTG 1 cut(s) 8
Cac8I GCNNGC 8 cut(s) 63, 325, 491, 571, 724, 737, 766, 1003
CaiI CAGNNNCTG 1 cut(s) 910
CfoI GCGC 4 cut(s) 407, 605, 764, 971
Cfr10I RCCGGY 2 cut(s) 323, 569
Cfr42I CCGCGG 1 cut(s) 580
Cfr9I CCCGGG 1 cut(s) 787
CseI GACGC 1 cut(s) 562
Csp6I GTAC 2 cut(s) 775, 1054
CviAII CATG 4 cut(s) 358, 502, 664, 931
CviQI GTAC 2 cut(s) 775, 1054
DdeI CTNAG 3 cut(s) 293, 758, 911
DpnI GATC 9 cut(s) 192, 434, 483, 500, 515, 662, 710, 756, 1010
DpnII GATC 9 cut(s) 190, 432, 481, 498, 513, 660, 708, 754, 1008
DraIII CACNNNGTG 1 cut(s) 934
DrdI GACNNNNNNGTC 1 cut(s) 347
DseDI GACNNNNNNGTC 1 cut(s) 347
EaeI YGGCCR 2 cut(s) 3, 714
EagI CGGCCG 1 cut(s) 714
Eam1104I CTCTTC 3 cut(s) 82, 290, 689
EarI CTCTTC 3 cut(s) 82, 290, 689
EciI GGCGGA 1 cut(s) 390
EclXI CGGCCG 1 cut(s) 714
Eco130I CCWWGG 2 cut(s) 525, 769
Eco24I GRGCYC 1 cut(s) 903
Eco31I GGTCTC 1 cut(s) 671
Eco47I GGWCC 4 cut(s) 649, 784, 802, 907
Eco52I CGGCCG 1 cut(s) 714
Eco72I CACGTG 1 cut(s) 920
Eco81I CCTNAGG 1 cut(s) 911
Eco88I CYCGRG 1 cut(s) 787
EcoO109I RGGNCCY 4 cut(s) 784, 864, 900, 907
EcoRII CCWGG 3 cut(s) 461, 669, 903
EcoT14I CCWWGG 2 cut(s) 525, 769
EcoT38I GRGCYC 1 cut(s) 903
ErhI CCWWGG 2 cut(s) 525, 769
Esp3I CGTCTC 1 cut(s) 550
FaeI CATG 4 cut(s) 361, 505, 667, 934
FaiI YATR 5 cut(s) 359, 503, 665, 887, 932
FaqI GGGAC 2 cut(s) 235, 797
FatI CATG 4 cut(s) 357, 501, 663, 930
FauI CCCGC 4 cut(s) 243, 260, 498, 574
FbaI TGATCA 2 cut(s) 660, 1008
FblI GTMKAC 2 cut(s) 354, 948
FokI GGATG 2 cut(s) 118, 1049
FriOI GRGCYC 1 cut(s) 903
GlaI GCGC 4 cut(s) 406, 604, 763, 970
GsaI CCCAGC 2 cut(s) 842, 879
GsuI CTGGAG 1 cut(s) 592
HaeII RGCGCY 1 cut(s) 765
HgaI GACGC 1 cut(s) 562
HhaI GCGC 4 cut(s) 407, 605, 764, 971
Hin1I GRCGYC 2 cut(s) 573, 951
Hin1II CATG 4 cut(s) 361, 505, 667, 934
Hin6I GCGC 4 cut(s) 405, 603, 762, 969
HinP1I GCGC 4 cut(s) 405, 603, 762, 969
HincII GTYRAC 3 cut(s) 355, 730, 949
HindII GTYRAC 3 cut(s) 355, 730, 949
HinfI GANTC 3 cut(s) 13, 378, 449
HphI GGTGA 3 cut(s) 37, 48, 142
Hpy166II GTNNAC 3 cut(s) 355, 730, 949
Hpy188I TCNGA 4 cut(s) 190, 385, 759, 1021
Hpy188III TCNNGA 2 cut(s) 508, 805
Hpy8I GTNNAC 3 cut(s) 355, 730, 949
Hpy99I CGWCG 9 cut(s) 217, 353, 356, 599, 626, 722, 950, 953, 956
HpyAV CCTTC 3 cut(s) 793, 811, 823
HpyCH4III ACNGT 2 cut(s) 208, 595
HpyCH4IV ACGT 3 cut(s) 919, 951, 984
HpyCH4V TGCA 2 cut(s) 739, 1043
HpyF3I CTNAG 3 cut(s) 293, 758, 911
HpySE526I ACGT 3 cut(s) 919, 951, 984
Hsp92I GRCGYC 2 cut(s) 573, 951
Hsp92II CATG 4 cut(s) 361, 505, 667, 934
HspAI GCGC 4 cut(s) 405, 603, 762, 969
KflI GGGWCCC 1 cut(s) 784
Kpn2I TCCGGA 1 cut(s) 804
KpnI GGTACC 1 cut(s) 778
KroI GCCGGC 2 cut(s) 323, 569
KroNI GCCGGC 2 cut(s) 325, 571
Ksp22I TGATCA 2 cut(s) 660, 1008
KspI CCGCGG 1 cut(s) 580
Kzo9I GATC 9 cut(s) 190, 432, 481, 498, 513, 660, 708, 754, 1008
LmnI GCTCC 6 cut(s) 361, 527, 587, 966, 1027, 1059
Lsp1109I GCAGC 2 cut(s) 429, 983
LweI GCATC 2 cut(s) 627, 1030
MaeII ACGT 3 cut(s) 919, 951, 984
MaeIII GTNAC 1 cut(s) 208
MalI GATC 9 cut(s) 192, 434, 483, 500, 515, 662, 710, 756, 1010
MbiI CCGCTC 2 cut(s) 61, 701
MboI GATC 9 cut(s) 190, 432, 481, 498, 513, 660, 708, 754, 1008
MboII GAAGA 3 cut(s) 69, 277, 706
MfeI CAATTG 1 cut(s) 653
MflI RGATCY 1 cut(s) 754
MhlI GDGCHC 3 cut(s) 366, 845, 903
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 640, 653
MluI ACGCGT 1 cut(s) 810
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 732
Mox20I TGGCCA 1 cut(s) 5
MreI CGCCGGCG 1 cut(s) 569
MroI TCCGGA 1 cut(s) 804
MroNI GCCGGC 2 cut(s) 323, 569
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 579, 974
MspR9I CCNGG 7 cut(s) 372, 428, 463, 671, 788, 789, 905
MunI CAATTG 1 cut(s) 653
MvaI CCWGG 3 cut(s) 463, 671, 905
MvnI CGCG 5 cut(s) 405, 579, 603, 713, 812
NaeI GCCGGC 2 cut(s) 325, 571
NciI CCSGG 4 cut(s) 372, 428, 788, 789
NdeII GATC 9 cut(s) 190, 432, 481, 498, 513, 660, 708, 754, 1008
NgoMIV GCCGGC 2 cut(s) 323, 569
NlaIII CATG 4 cut(s) 361, 505, 667, 934
NmeAIII GCCGAG 4 cut(s) 340, 465, 559, 850
PcsI WCGNNNNNNNCGW 2 cut(s) 345, 621
PdiI GCCGGC 2 cut(s) 325, 571
PfeI GAWTC 2 cut(s) 378, 449
PflFI GACNNNGTC 1 cut(s) 344
PleI GAGTC 1 cut(s) 7
PmaCI CACGTG 1 cut(s) 920
PmlI CACGTG 1 cut(s) 920
PpsI GAGTC 1 cut(s) 7
Ppu21I YACGTR 1 cut(s) 920
PpuMI RGGWCCY 2 cut(s) 784, 907
Psp5II RGGWCCY 2 cut(s) 784, 907
Psp6I CCWGG 3 cut(s) 461, 669, 903
PspCI CACGTG 1 cut(s) 920
PspFI CCCAGC 2 cut(s) 838, 875
PspGI CCWGG 3 cut(s) 461, 669, 903
PspOMI GGGCCC 1 cut(s) 899
PspPPI RGGWCCY 2 cut(s) 784, 907
PstNI CAGNNNCTG 1 cut(s) 910
PsuI RGATCY 1 cut(s) 754
PsyI GACNNNGTC 1 cut(s) 344
RsaI GTAC 2 cut(s) 776, 1055
RsaNI GTAC 2 cut(s) 775, 1054
SacII CCGCGG 1 cut(s) 580
SalI GTCGAC 2 cut(s) 353, 947
Sau3AI GATC 9 cut(s) 190, 432, 481, 498, 513, 660, 708, 754, 1008
ScaI AGTACT 1 cut(s) 1055
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 7 cut(s) 372, 428, 463, 671, 788, 789, 905
SduI GDGCHC 3 cut(s) 366, 845, 903
SfaNI GCATC 2 cut(s) 627, 1030
Sfr303I CCGCGG 1 cut(s) 580
SgrAI CRCCGGYG 1 cut(s) 569
SgrBI CCGCGG 1 cut(s) 580
SgrDI CGTCGACG 1 cut(s) 947
SinI GGWCC 4 cut(s) 649, 784, 802, 907
SmaI CCCGGG 1 cut(s) 789
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 2 cut(s) 640, 653
StyD4I CCNGG 7 cut(s) 370, 426, 461, 669, 786, 787, 903
StyI CCWWGG 2 cut(s) 525, 769
TaaI ACNGT 2 cut(s) 208, 595
TaiI ACGT 3 cut(s) 922, 954, 987
TaqI TCGA 5 cut(s) 354, 480, 516, 621, 948
TaqII GACCGA 1 cut(s) 695
TasI AATT 2 cut(s) 640, 653
TatI WGTACW 1 cut(s) 1053
TauI GCSGC 9 cut(s) 23, 26, 29, 32, 35, 416, 558, 582, 716
TfiI GAWTC 2 cut(s) 378, 449
TscAI CASTG 1 cut(s) 15
TseI GCWGC 2 cut(s) 442, 971
TspGWI ACGGA 1 cut(s) 934
TspMI CCCGGG 1 cut(s) 787
TspRI CASTG 1 cut(s) 15
Tth111I GACNNNGTC 1 cut(s) 344
VpaK11BI GGWCC 4 cut(s) 649, 784, 802, 907
XmaI CCCGGG 1 cut(s) 787
XmiI GTMKAC 2 cut(s) 354, 948
ZraI GACGTC 1 cut(s) 952
ZrmI AGTACT 1 cut(s) 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.