Rh7AG063900

HpcH/HpaI aldolase/citrate lyase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0012383
Physical Location & Seq
Forward (+)
4418358 .. 4419518
1161 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG063900.1

Sequence Viewer

Length: 1161 bp
ATGACACTCTCAGTCTCTCACTACTCAACAGTTCATTTAGGTCAGTATAGCGTACAGTATACAACTCCAAATATTCTTCGACAATCAATCCCAAAGAGCAAAGCAGCCATGGCCACACTGACTCACGCCGCCGCCGCCACCGCCTCATCACCGCTCGCAAAACCCATCTCTTCCAGTCCCACCACCACCAATCCCCTATCCCATCTCTCTTTCCCTTTCTCCCCATCCAAAACCCTAAACCTCACCGCCAAAACCACCTCAATCAAATCCTCCTCCTCCTCCGATCTCTCCACCACCACCGTAACGACCACCTCCATCTCCTCCTCCCCGCAAACCCTCAAGTCCCGCCTCCACAATGGCGACACCCTCTACGGCCTCTTCCTCCTCAGCTTCTCCCCCACCCTCGCCGAGATCGCCGGCCTCTCCGGCTACGACTTTGTCGTCGTCGACATGGAGCACGGCCCCGGCGGAATCTCCGAAGCCCTCTCCTGCCTCCGCGCCCTCGCCGCCACCCAAACCCCGGCGATCCTCCGGCTGCCGGAATCCTCCCCGACCTGGGCCAAGAAAGCCCTCGATCTCGGCCCGCAGGGGATCATGTTCCCGATGATCGACAGCTCCAAGGAGGCCGAGAAAGCCGTCTCCTACTGCCGCTTCCCTCCCGCCGGCGTCCGCGGCTCCGCACACACCGTCGTCCGCGCCTCCGGCTACGGCATCGACGAGGGGTATTTGAGTAATTACGAGGACCAATTGCTGATCATGTGCCAGGTGGAGACCGAGGAGGGCGTGAAGAGAGCGGAGGAGATCGCGGCCGTCGAAGGCGTTGACTGCGTGCAGATGGGGCCGTTGGATCTGAGCGCCAGCCTTGGGTACCTGTGGGACCCGGGGAACAAGAAGGTCCGGAACGCGTTGAAGGTTGCCGAGAAGGCCGTGCTGGGCACCGACCCCCTTGTGGGTGGGGCCTACTTGGCTGGGTTCGCTATGCCCCACGATGGGCCCCAGGACCTGAGGACACGTGGCTACCACATGGTGTCCGGTTCCGTCGACGTCGGGCTGTTTAGGAGCGCAGCGGTGGAGGACGTGAGGAGGTTTCAGGCGAGCTTGATCAACGGCTCTGATGAGGAGCGTGGGGATGATGCAGATGAGAAGTACTGGAGCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HpcH_HpaI

Protein Analysis

386

Amino Acids

41.06

Weight (kDa)

5.64

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HpcH_HpaI PF03328 124 - 354 2.9e-54 HpcH/HpaI aldolase/citrate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10750
fragaria_vesca FvH4_5g11021 FvH4_5g11021
malus_domestica MD06G1117400.v1.1
prunus_persica Prupe.5G128500_v2.0.a1
pyrus_communis pycom06g10930
rosa_chinensis RchiOBHm_Chr7g0186781
rosa_laevigata RLG00000004816
rosa_multiflora Rmu_co8215596.1_g000001 Rmu_sc0009734.1_g000001 Rmu_sc0040245.1_g000001 Rmu_ssc0000024.1_g000045
rosa_roxburghii Rroxscaffold_3G00267830
rosa_rugosa Rorug06G0476600
rosa_samantha Rh7AG063900 Rh7BG080400 Rh7CG083600 Rh7DG084500
rosa_wichuraiana Rw7G007250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 440
AatII GACGTC 1 cut(s) 1047
Acc65I GGTACC 1 cut(s) 867
AccB1I GGYRCC 2 cut(s) 867, 935
AccBSI CCGCTC 2 cut(s) 154, 794
AccI GTMKAC 3 cut(s) 59, 447, 1041
AccII CGCG 5 cut(s) 498, 672, 696, 806, 905
AccIII TCCGGA 1 cut(s) 897
AclWI GGATC 3 cut(s) 520, 599, 855
AcoI YGGCCR 2 cut(s) 111, 807
AcvI CACGTG 1 cut(s) 1013
AcyI GRCGYC 2 cut(s) 666, 1044
AdeI CACNNNGTG 1 cut(s) 1027
AfaI GTAC 3 cut(s) 54, 869, 1148
AflIII ACRYGT 2 cut(s) 903, 1010
AgsI TTSAA 1 cut(s) 910
AjiI CACGTC 1 cut(s) 1078
AjnI CCWGG 3 cut(s) 554, 762, 996
AluBI AGCT 3 cut(s) 390, 615, 1098
AluI AGCT 3 cut(s) 390, 615, 1098
Alw21I GWGCWC 1 cut(s) 459
Alw26I GTCTC 3 cut(s) 19, 643, 764
AlwI GGATC 3 cut(s) 520, 599, 855
AlwNI CAGNNNCTG 1 cut(s) 1003
Ama87I CYCGRG 1 cut(s) 880
Aor13HI TCCGGA 1 cut(s) 897
ApaI GGGCCC 1 cut(s) 996
ApeKI GCWGC 3 cut(s) 104, 535, 1064
Asp718I GGTACC 1 cut(s) 867
AspLEI GCGC 4 cut(s) 500, 698, 857, 1064
AsuC2I CCSGG 4 cut(s) 465, 521, 881, 882
AsuHPI GGTGA 2 cut(s) 141, 235
AvaI CYCGRG 1 cut(s) 880
AvaII GGWCC 4 cut(s) 742, 877, 895, 1000
AxyI CCTNAGG 1 cut(s) 1004
BaeGI GKGCMC 2 cut(s) 938, 996
BalI TGGCCA 1 cut(s) 113
BanI GGYRCC 2 cut(s) 867, 935
BanII GRGCYC 1 cut(s) 996
BbrPI CACGTG 1 cut(s) 1013
Bbv12I GWGCWC 1 cut(s) 459
BbvCI CCTCAGC 1 cut(s) 386
BbvI GCAGC 3 cut(s) 116, 522, 1076
BccI CCATC 6 cut(s) 173, 210, 232, 323, 829, 983
BceAI ACGGC 8 cut(s) 388, 475, 620, 724, 794, 826, 911, 1123
BciT130I CCWGG 3 cut(s) 556, 764, 998
BclI TGATCA 2 cut(s) 753, 1101
BcnI CCSGG 4 cut(s) 465, 521, 881, 882
BcoDI GTCTC 3 cut(s) 19, 643, 764
BfoI RGCGCY 1 cut(s) 858
BglI GCCNNNNNGGC 3 cut(s) 426, 923, 965
BmcAI AGTACT 1 cut(s) 1148
Bme1390I CCNGG 7 cut(s) 465, 521, 556, 764, 881, 882, 998
Bme18I GGWCC 4 cut(s) 742, 877, 895, 1000
BmeT110I CYCGRG 1 cut(s) 880
BmgBI CACGTC 1 cut(s) 1078
BmrFI CCNGG 7 cut(s) 465, 521, 556, 764, 881, 882, 998
BmsI GCATC 2 cut(s) 720, 1123
Bpu10I CCTNAGC 1 cut(s) 386
BpuEI CTTGAG 1 cut(s) 323
BpuMI CCSGG 4 cut(s) 465, 521, 881, 882
BsaAI YACGTR 1 cut(s) 1013
BsaHI GRCGYC 2 cut(s) 666, 1044
BsaI GGTCTC 1 cut(s) 764
BsaWI WCCGGW 2 cut(s) 897, 1031
Bse118I RCCGGY 2 cut(s) 416, 662
Bse1I ACTGG 2 cut(s) 174, 1154
Bse21I CCTNAGG 1 cut(s) 1004
BseAI TCCGGA 1 cut(s) 897
BseBI CCWGG 3 cut(s) 556, 764, 998
BseGI GGATG 2 cut(s) 224, 1135
BseMII CTCAG 4 cut(s) 24, 400, 842, 995
BseNI ACTGG 2 cut(s) 174, 1154
BseSI GKGCMC 2 cut(s) 938, 996
BseX3I CGGCCG 1 cut(s) 807
BseXI GCAGC 3 cut(s) 116, 522, 1076
BseYI CCCAGC 2 cut(s) 931, 968
BsgI GTGCAG 1 cut(s) 851
Bsh1236I CGCG 5 cut(s) 498, 672, 696, 806, 905
Bsh1285I CGRYCG 1 cut(s) 810
BshNI GGYRCC 2 cut(s) 867, 935
BsiEI CGRYCG 1 cut(s) 810
BsiHKAI GWGCWC 1 cut(s) 459
BsiHKCI CYCGRG 1 cut(s) 880
BslFI GGGAC 3 cut(s) 162, 328, 890
BsmAI GTCTC 3 cut(s) 19, 643, 764
BsmBI CGTCTC 1 cut(s) 643
BsmFI GGGAC 3 cut(s) 162, 328, 890
Bso31I GGTCTC 1 cut(s) 764
BsoBI CYCGRG 1 cut(s) 880
Bsp120I GGGCCC 1 cut(s) 992
Bsp1286I GDGCHC 3 cut(s) 459, 938, 996
Bsp13I TCCGGA 1 cut(s) 897
Bsp19I CCATGG 1 cut(s) 108
BspCNI CTCAG 4 cut(s) 23, 399, 843, 996
BspEI TCCGGA 1 cut(s) 897
BspFNI CGCG 5 cut(s) 498, 672, 696, 806, 905
BspPI GGATC 3 cut(s) 520, 599, 855
BspT107I GGYRCC 2 cut(s) 867, 935
BspTNI GGTCTC 1 cut(s) 764
BsrBI CCGCTC 2 cut(s) 154, 794
BsrFI RCCGGY 2 cut(s) 416, 662
BsrI ACTGG 2 cut(s) 174, 1154
BssAI RCCGGY 2 cut(s) 416, 662
BssNAI GTATAC 1 cut(s) 60
BssNI GRCGYC 2 cut(s) 666, 1044
BssT1I CCWWGG 3 cut(s) 108, 618, 862
Bst1107I GTATAC 1 cut(s) 60
Bst2UI CCWGG 3 cut(s) 556, 764, 998
Bst4CI ACNGT 4 cut(s) 31, 57, 301, 688
Bst6I CTCTTC 3 cut(s) 175, 383, 782
BstACI GRCGYC 2 cut(s) 666, 1044
BstBAI YACGTR 1 cut(s) 1013
BstC8I GCNNGC 7 cut(s) 156, 418, 584, 664, 830, 859, 1096
BstDEI CTNAG 4 cut(s) 10, 386, 851, 1004
BstDSI CCRYGG 2 cut(s) 108, 670
BstF5I GGATG 2 cut(s) 224, 1135
BstFNI CGCG 5 cut(s) 498, 672, 696, 806, 905
BstH2I RGCGCY 1 cut(s) 858
BstHHI GCGC 4 cut(s) 500, 698, 857, 1064
BstMAI GTCTC 3 cut(s) 19, 643, 764
BstMCI CGRYCG 1 cut(s) 810
BstNI CCWGG 3 cut(s) 556, 764, 998
BstSCI CCNGG 7 cut(s) 463, 519, 554, 762, 879, 880, 996
BstSLI GKGCMC 2 cut(s) 938, 996
BstUI CGCG 5 cut(s) 498, 672, 696, 806, 905
BstV1I GCAGC 3 cut(s) 116, 522, 1076
BstX2I RGATCY 1 cut(s) 847
BstYI RGATCY 1 cut(s) 847
BstZ17I GTATAC 1 cut(s) 60
BstZI CGGCCG 1 cut(s) 807
Bsu36I CCTNAGG 1 cut(s) 1004
BtgI CCRYGG 2 cut(s) 108, 670
BtrI CACGTC 1 cut(s) 1078
BtsCI GGATG 2 cut(s) 224, 1135
BtsIMutI CAGTG 1 cut(s) 116
Cac8I GCNNGC 7 cut(s) 156, 418, 584, 664, 830, 859, 1096
CaiI CAGNNNCTG 1 cut(s) 1003
CfoI GCGC 4 cut(s) 500, 698, 857, 1064
Cfr10I RCCGGY 2 cut(s) 416, 662
Cfr42I CCGCGG 1 cut(s) 673
Cfr9I CCCGGG 1 cut(s) 880
CseI GACGC 1 cut(s) 655
Csp6I GTAC 3 cut(s) 53, 868, 1147
CviAII CATG 5 cut(s) 109, 451, 595, 757, 1024
CviQI GTAC 3 cut(s) 53, 868, 1147
DdeI CTNAG 4 cut(s) 10, 386, 851, 1004
DraIII CACNNNGTG 1 cut(s) 1027
DrdI GACNNNNNNGTC 1 cut(s) 440
DseDI GACNNNNNNGTC 1 cut(s) 440
EaeI YGGCCR 2 cut(s) 111, 807
EagI CGGCCG 1 cut(s) 807
Eam1104I CTCTTC 3 cut(s) 175, 383, 782
EarI CTCTTC 3 cut(s) 175, 383, 782
EciI GGCGGA 1 cut(s) 483
EclXI CGGCCG 1 cut(s) 807
Eco130I CCWWGG 3 cut(s) 108, 618, 862
Eco24I GRGCYC 1 cut(s) 996
Eco31I GGTCTC 1 cut(s) 764
Eco47I GGWCC 4 cut(s) 742, 877, 895, 1000
Eco52I CGGCCG 1 cut(s) 807
Eco72I CACGTG 1 cut(s) 1013
Eco81I CCTNAGG 1 cut(s) 1004
Eco88I CYCGRG 1 cut(s) 880
EcoO109I RGGNCCY 4 cut(s) 877, 957, 993, 1000
EcoRII CCWGG 3 cut(s) 554, 762, 996
EcoT14I CCWWGG 3 cut(s) 108, 618, 862
EcoT38I GRGCYC 1 cut(s) 996
ErhI CCWWGG 3 cut(s) 108, 618, 862
Esp3I CGTCTC 1 cut(s) 643
FaeI CATG 5 cut(s) 112, 454, 598, 760, 1027
FaiI YATR 8 cut(s) 48, 60, 110, 452, 596, 758, 980, 1025
FaqI GGGAC 3 cut(s) 162, 328, 890
FatI CATG 5 cut(s) 108, 450, 594, 756, 1023
FauI CCCGC 4 cut(s) 336, 353, 591, 667
FbaI TGATCA 2 cut(s) 753, 1101
FblI GTMKAC 3 cut(s) 59, 447, 1041
FokI GGATG 2 cut(s) 211, 1142
FriOI GRGCYC 1 cut(s) 996
GlaI GCGC 4 cut(s) 499, 697, 856, 1063
GsaI CCCAGC 2 cut(s) 935, 972
HaeII RGCGCY 1 cut(s) 858
HgaI GACGC 1 cut(s) 655
HhaI GCGC 4 cut(s) 500, 698, 857, 1064
Hin1I GRCGYC 2 cut(s) 666, 1044
Hin1II CATG 5 cut(s) 112, 454, 598, 760, 1027
Hin6I GCGC 4 cut(s) 498, 696, 855, 1062
HinP1I GCGC 4 cut(s) 498, 696, 855, 1062
HincII GTYRAC 3 cut(s) 448, 823, 1042
HindII GTYRAC 3 cut(s) 448, 823, 1042
HinfI GANTC 3 cut(s) 121, 471, 542
HphI GGTGA 2 cut(s) 141, 235
Hpy166II GTNNAC 4 cut(s) 60, 448, 823, 1042
Hpy188I TCNGA 4 cut(s) 283, 478, 852, 1114
Hpy188III TCNNGA 2 cut(s) 601, 898
Hpy8I GTNNAC 4 cut(s) 60, 448, 823, 1042
Hpy99I CGWCG 8 cut(s) 446, 449, 692, 719, 815, 1043, 1046, 1049
HpyAV CCTTC 4 cut(s) 809, 886, 904, 916
HpyCH4III ACNGT 4 cut(s) 31, 57, 301, 688
HpyCH4IV ACGT 3 cut(s) 1012, 1044, 1077
HpyCH4V TGCA 2 cut(s) 832, 1136
HpyF3I CTNAG 4 cut(s) 10, 386, 851, 1004
HpySE526I ACGT 3 cut(s) 1012, 1044, 1077
Hsp92I GRCGYC 2 cut(s) 666, 1044
Hsp92II CATG 5 cut(s) 112, 454, 598, 760, 1027
HspAI GCGC 4 cut(s) 498, 696, 855, 1062
KflI GGGWCCC 1 cut(s) 877
Kpn2I TCCGGA 1 cut(s) 897
KpnI GGTACC 1 cut(s) 871
KroI GCCGGC 2 cut(s) 416, 662
KroNI GCCGGC 2 cut(s) 418, 664
Ksp22I TGATCA 2 cut(s) 753, 1101
KspI CCGCGG 1 cut(s) 673
LmnI GCTCC 6 cut(s) 454, 620, 680, 1059, 1120, 1152
Lsp1109I GCAGC 3 cut(s) 116, 522, 1076
LweI GCATC 2 cut(s) 720, 1123
MaeII ACGT 3 cut(s) 1012, 1044, 1077
MaeIII GTNAC 1 cut(s) 301
MbiI CCGCTC 2 cut(s) 154, 794
MboII GAAGA 4 cut(s) 68, 162, 370, 799
MfeI CAATTG 1 cut(s) 746
MflI RGATCY 1 cut(s) 847
MhlI GDGCHC 3 cut(s) 459, 938, 996
MlsI TGGCCA 1 cut(s) 113
MluCI AATT 2 cut(s) 733, 746
MluI ACGCGT 1 cut(s) 903
MluNI TGGCCA 1 cut(s) 113
MlyI GAGTC 1 cut(s) 115
MmeI TCCRAC 1 cut(s) 825
Mox20I TGGCCA 1 cut(s) 113
MreI CGCCGGCG 1 cut(s) 662
MroI TCCGGA 1 cut(s) 897
MroNI GCCGGC 2 cut(s) 416, 662
MscI TGGCCA 1 cut(s) 113
Msp20I TGGCCA 1 cut(s) 113
MspA1I CMGCKG 2 cut(s) 672, 1067
MspR9I CCNGG 7 cut(s) 465, 521, 556, 764, 881, 882, 998
MunI CAATTG 1 cut(s) 746
MvaI CCWGG 3 cut(s) 556, 764, 998
MvnI CGCG 5 cut(s) 498, 672, 696, 806, 905
NaeI GCCGGC 2 cut(s) 418, 664
NciI CCSGG 4 cut(s) 465, 521, 881, 882
NcoI CCATGG 1 cut(s) 108
NgoMIV GCCGGC 2 cut(s) 416, 662
NlaIII CATG 5 cut(s) 112, 454, 598, 760, 1027
NmeAIII GCCGAG 4 cut(s) 433, 558, 652, 943
PcsI WCGNNNNNNNCGW 3 cut(s) 438, 714, 810
PdiI GCCGGC 2 cut(s) 418, 664
PfeI GAWTC 2 cut(s) 471, 542
PflFI GACNNNGTC 1 cut(s) 437
PleI GAGTC 1 cut(s) 115
PmaCI CACGTG 1 cut(s) 1013
PmlI CACGTG 1 cut(s) 1013
PpsI GAGTC 1 cut(s) 115
Ppu21I YACGTR 1 cut(s) 1013
PpuMI RGGWCCY 2 cut(s) 877, 1000
Psp5II RGGWCCY 2 cut(s) 877, 1000
Psp6I CCWGG 3 cut(s) 554, 762, 996
PspCI CACGTG 1 cut(s) 1013
PspFI CCCAGC 2 cut(s) 931, 968
PspGI CCWGG 3 cut(s) 554, 762, 996
PspOMI GGGCCC 1 cut(s) 992
PspPPI RGGWCCY 2 cut(s) 877, 1000
PstNI CAGNNNCTG 1 cut(s) 1003
PsuI RGATCY 1 cut(s) 847
PsyI GACNNNGTC 1 cut(s) 437
RsaI GTAC 3 cut(s) 54, 869, 1148
RsaNI GTAC 3 cut(s) 53, 868, 1147
SacII CCGCGG 1 cut(s) 673
SalI GTCGAC 2 cut(s) 446, 1040
ScaI AGTACT 1 cut(s) 1148
SchI GAGTC 1 cut(s) 115
ScrFI CCNGG 7 cut(s) 465, 521, 556, 764, 881, 882, 998
SduI GDGCHC 3 cut(s) 459, 938, 996
SfaNI GCATC 2 cut(s) 720, 1123
Sfr303I CCGCGG 1 cut(s) 673
SgrAI CRCCGGYG 1 cut(s) 662
SgrBI CCGCGG 1 cut(s) 673
SgrDI CGTCGACG 1 cut(s) 1040
SinI GGWCC 4 cut(s) 742, 877, 895, 1000
SmaI CCCGGG 1 cut(s) 882
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
Sse9I AATT 2 cut(s) 733, 746
SspI AATATT 1 cut(s) 73
StyD4I CCNGG 7 cut(s) 463, 519, 554, 762, 879, 880, 996
StyI CCWWGG 3 cut(s) 108, 618, 862
TaaI ACNGT 4 cut(s) 31, 57, 301, 688
TaiI ACGT 3 cut(s) 1015, 1047, 1080
TaqI TCGA 7 cut(s) 79, 447, 573, 609, 714, 813, 1041
TaqII GACCGA 1 cut(s) 788
TasI AATT 2 cut(s) 733, 746
TatI WGTACW 1 cut(s) 1146
TauI GCSGC 7 cut(s) 131, 134, 137, 509, 651, 675, 809
TfiI GAWTC 2 cut(s) 471, 542
TscAI CASTG 1 cut(s) 123
TseI GCWGC 3 cut(s) 104, 535, 1064
TspDTI ATGAA 1 cut(s) 23
TspGWI ACGGA 1 cut(s) 1027
TspMI CCCGGG 1 cut(s) 880
TspRI CASTG 1 cut(s) 123
Tth111I GACNNNGTC 1 cut(s) 437
VpaK11BI GGWCC 4 cut(s) 742, 877, 895, 1000
XmaI CCCGGG 1 cut(s) 880
XmiI GTMKAC 3 cut(s) 59, 447, 1041
ZraI GACGTC 1 cut(s) 1045
ZrmI AGTACT 1 cut(s) 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.