RLG00000004816

HpcH/HpaI aldolase/citrate lyase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012383
Physical Location & Seq
Forward (+)
62773950 .. 62775328
1379 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004816

Sequence Viewer

Length: 1050 bp
ATGGCCACACTGACTCACGCCTCCGCCTCATCACCTCTCGGAAAACCCATCTCTTCTAGTCCCACCACCACCAAAGCCCTATCCCATCTCTCTTTCCCTTTCTCCCCATCCAAAACCCTAAACCTCACCGCCAAAACCACCTCAATCAAATCATCCTCCTCCGATCTCTCCACCACCACCGTCAACACCACCTCCATCTCCTCCTCCCCGCAAACCCTCAAGTCCCGCCTCCACAATGGCGACACCCTCTACGGCCTCTTCCTCCTCAGCTTCTCCCCCACCCTCGCCGAGATCGCCGGCCTCTCCGGCTACGACTTCGTCGTCGTCGACATGGAGCACGGCCCCGGCGGAATCTCCGAAGCCCTCTCCTGCCTCCGCGCCCTCGCCGCCACCCAAACCCCGGCGATCCTCCGGCTGCCGGAATCCTCCGCGACCTGGGCCAAGAAAGCCCTAGATCTCGGCCCGCAGGGTATCATGTTCCCGATGATAGACAGCTCCAAGGAGGCCCAGAAAGCCGTCTCCTACTGCCGCTTCCCTCCCGCCGGAATCCGCGGCTCCGCCCACACCGTCGTCCGCGCCTCCAGCTACGGCATCGACGAGGGGTATTTGAGTAATTACGAGGACCAATTGCTGATCATGTGCCAGGTGGAGAGCGAGGAGGGCGTGAAGCGAGCGGAGGAGATCGCGGCCGTCGAAGGAGTTGACTGCGTGCAGATTGGGCCGTTGGATCTGAGCGCCAGCTTGGGGTACCTGTGGGACCCGGGGAACAAGAAGGTCCGGAGCGCGTTGAAGGTTGCCGAGAAGGCCGTGCTGGGCACCGACCCCCTTGTGGGTGGGGCCTACTTGGCTGGGTTCGCTATGCCCCACGATGGGCCCCAGGACCTGAGGACACGTGGCTACCACATGGTGTCCGGTTCCGTCGACGTCGGGCTGTTTAGGAGCGCAGCGGTGGAGGACGTGAGGAGGTTTCAGGCGAGCTTGATCAACGGCTCTGATGAGGAGGAGGGGGAGCATGGTGATGATGCAGATGAGAAGTACTGGAGCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HpcH_HpaI

Protein Analysis

350

Amino Acids

37.0

Weight (kDa)

5.17

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HpcH_HpaI PF03328 84 - 314 1.4e-54 HpcH/HpaI aldolase/citrate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10750
fragaria_vesca FvH4_5g11021 FvH4_5g11021
malus_domestica MD06G1117400.v1.1
prunus_persica Prupe.5G128500_v2.0.a1
pyrus_communis pycom06g10930
rosa_chinensis RchiOBHm_Chr7g0186781
rosa_laevigata RLG00000004816
rosa_multiflora Rmu_co8215596.1_g000001 Rmu_sc0009734.1_g000001 Rmu_sc0040245.1_g000001 Rmu_ssc0000024.1_g000045
rosa_roxburghii Rroxscaffold_3G00267830
rosa_rugosa Rorug06G0476600
rosa_samantha Rh7AG063900 Rh7BG080400 Rh7CG083600 Rh7DG084500
rosa_wichuraiana Rw7G007250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 320
AatII GACGTC 1 cut(s) 927
Acc65I GGTACC 1 cut(s) 747
AccB1I GGYRCC 2 cut(s) 747, 815
AccBSI CCGCTC 1 cut(s) 674
AccI GTMKAC 2 cut(s) 327, 921
AccII CGCG 6 cut(s) 378, 431, 552, 576, 686, 785
AccIII TCCGGA 1 cut(s) 777
AclWI GGATC 2 cut(s) 400, 735
AcoI YGGCCR 2 cut(s) 3, 687
AcvI CACGTG 1 cut(s) 893
AcyI GRCGYC 1 cut(s) 924
AdeI CACNNNGTG 1 cut(s) 907
AfaI GTAC 2 cut(s) 749, 1037
AfiI CCNNNNNNNGG 9 cut(s) 400, 418, 435, 542, 744, 829, 830, 869, 870
AflIII ACRYGT 1 cut(s) 890
AgsI TTSAA 1 cut(s) 790
AjiI CACGTC 1 cut(s) 958
AjnI CCWGG 3 cut(s) 434, 642, 876
AluBI AGCT 5 cut(s) 270, 495, 585, 741, 978
AluI AGCT 5 cut(s) 270, 495, 585, 741, 978
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 1 cut(s) 523
AlwI GGATC 2 cut(s) 400, 735
AlwNI CAGNNNCTG 1 cut(s) 883
Ama87I CYCGRG 1 cut(s) 760
Aor13HI TCCGGA 1 cut(s) 777
ApaI GGGCCC 1 cut(s) 876
ApeKI GCWGC 2 cut(s) 415, 944
Asp718I GGTACC 1 cut(s) 747
AspLEI GCGC 5 cut(s) 380, 578, 737, 785, 944
AsuC2I CCSGG 4 cut(s) 345, 401, 761, 762
AsuHPI GGTGA 3 cut(s) 24, 118, 1028
AvaI CYCGRG 1 cut(s) 760
AvaII GGWCC 4 cut(s) 622, 757, 775, 880
AxyI CCTNAGG 1 cut(s) 884
BaeGI GKGCMC 2 cut(s) 818, 876
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 747, 815
BanII GRGCYC 1 cut(s) 876
BbrPI CACGTG 1 cut(s) 893
Bbv12I GWGCWC 1 cut(s) 339
BbvCI CCTCAGC 1 cut(s) 266
BbvI GCAGC 2 cut(s) 402, 956
BccI CCATC 5 cut(s) 56, 93, 115, 203, 863
BceAI ACGGC 8 cut(s) 268, 355, 500, 604, 674, 706, 791, 1003
BciT130I CCWGG 3 cut(s) 436, 644, 878
BclI TGATCA 2 cut(s) 633, 981
BcnI CCSGG 4 cut(s) 345, 401, 761, 762
BcoDI GTCTC 1 cut(s) 523
BfaI CTAG 2 cut(s) 57, 452
BfoI RGCGCY 1 cut(s) 738
BglI GCCNNNNNGGC 3 cut(s) 306, 803, 845
BglII AGATCT 1 cut(s) 454
BisI GCNGC 6 cut(s) 387, 416, 529, 553, 687, 945
BlsI GCNGC 6 cut(s) 388, 417, 530, 554, 688, 946
BmcAI AGTACT 1 cut(s) 1037
Bme1390I CCNGG 7 cut(s) 345, 401, 436, 644, 761, 762, 878
Bme18I GGWCC 4 cut(s) 622, 757, 775, 880
BmeT110I CYCGRG 1 cut(s) 760
BmgBI CACGTC 1 cut(s) 958
BmrFI CCNGG 7 cut(s) 345, 401, 436, 644, 761, 762, 878
BmsI GCATC 2 cut(s) 600, 1012
BpmI CTGGAG 1 cut(s) 565
Bpu10I CCTNAGC 1 cut(s) 266
BpuEI CTTGAG 1 cut(s) 203
BpuMI CCSGG 4 cut(s) 345, 401, 761, 762
BsaAI YACGTR 1 cut(s) 893
BsaHI GRCGYC 1 cut(s) 924
BsaJI CCNNGG 8 cut(s) 343, 399, 435, 498, 550, 760, 761, 876
BsaWI WCCGGW 2 cut(s) 777, 911
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 9 cut(s) 400, 418, 435, 542, 744, 829, 830, 869, 870
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 1 cut(s) 1043
Bse21I CCTNAGG 1 cut(s) 884
BseAI TCCGGA 1 cut(s) 777
BseBI CCWGG 3 cut(s) 436, 644, 878
BseDI CCNNGG 8 cut(s) 343, 399, 435, 498, 550, 760, 761, 876
BseGI GGATG 2 cut(s) 107, 152
BseLI CCNNNNNNNGG 9 cut(s) 400, 418, 435, 542, 744, 829, 830, 869, 870
BseMII CTCAG 3 cut(s) 280, 722, 875
BseNI ACTGG 1 cut(s) 1043
BseRI GAGGAG 9 cut(s) 148, 190, 193, 254, 671, 692, 976, 1013, 1016
BseSI GKGCMC 2 cut(s) 818, 876
BseX3I CGGCCG 1 cut(s) 687
BseXI GCAGC 2 cut(s) 402, 956
BseYI CCCAGC 2 cut(s) 811, 848
BsgI GTGCAG 1 cut(s) 731
Bsh1236I CGCG 6 cut(s) 378, 431, 552, 576, 686, 785
Bsh1285I CGRYCG 1 cut(s) 690
BshNI GGYRCC 2 cut(s) 747, 815
BsiEI CGRYCG 1 cut(s) 690
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 1 cut(s) 760
BslFI GGGAC 3 cut(s) 45, 208, 770
BslI CCNNNNNNNGG 9 cut(s) 400, 418, 435, 542, 744, 829, 830, 869, 870
BsmAI GTCTC 1 cut(s) 523
BsmBI CGTCTC 1 cut(s) 523
BsmFI GGGAC 3 cut(s) 45, 208, 770
BsoBI CYCGRG 1 cut(s) 760
Bsp120I GGGCCC 1 cut(s) 872
Bsp1286I GDGCHC 3 cut(s) 339, 818, 876
Bsp13I TCCGGA 1 cut(s) 777
Bsp143I GATC 8 cut(s) 163, 291, 405, 454, 633, 681, 727, 981
BspCNI CTCAG 3 cut(s) 279, 723, 876
BspEI TCCGGA 1 cut(s) 777
BspFNI CGCG 6 cut(s) 378, 431, 552, 576, 686, 785
BspPI GGATC 2 cut(s) 400, 735
BspT107I GGYRCC 2 cut(s) 747, 815
BsrBI CCGCTC 1 cut(s) 674
BsrFI RCCGGY 1 cut(s) 296
BsrI ACTGG 1 cut(s) 1043
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 8 cut(s) 343, 399, 435, 498, 550, 760, 761, 876
BssMI GATC 8 cut(s) 163, 291, 405, 454, 633, 681, 727, 981
BssNI GRCGYC 1 cut(s) 924
BssT1I CCWWGG 1 cut(s) 498
Bst2UI CCWGG 3 cut(s) 436, 644, 878
Bst4CI ACNGT 2 cut(s) 181, 568
Bst6I CTCTTC 2 cut(s) 58, 263
BstACI GRCGYC 1 cut(s) 924
BstBAI YACGTR 1 cut(s) 893
BstC8I GCNNGC 6 cut(s) 298, 464, 672, 710, 739, 976
BstDEI CTNAG 3 cut(s) 266, 731, 884
BstDSI CCRYGG 1 cut(s) 550
BstF5I GGATG 2 cut(s) 107, 152
BstFNI CGCG 6 cut(s) 378, 431, 552, 576, 686, 785
BstH2I RGCGCY 1 cut(s) 738
BstHHI GCGC 5 cut(s) 380, 578, 737, 785, 944
BstKTI GATC 8 cut(s) 166, 294, 408, 457, 636, 684, 730, 984
BstMAI GTCTC 1 cut(s) 523
BstMBI GATC 8 cut(s) 163, 291, 405, 454, 633, 681, 727, 981
BstMCI CGRYCG 1 cut(s) 690
BstNI CCWGG 3 cut(s) 436, 644, 878
BstSCI CCNGG 7 cut(s) 343, 399, 434, 642, 759, 760, 876
BstSLI GKGCMC 2 cut(s) 818, 876
BstUI CGCG 6 cut(s) 378, 431, 552, 576, 686, 785
BstV1I GCAGC 2 cut(s) 402, 956
BstX2I RGATCY 2 cut(s) 454, 727
BstYI RGATCY 2 cut(s) 454, 727
BstZI CGGCCG 1 cut(s) 687
Bsu36I CCTNAGG 1 cut(s) 884
BtgI CCRYGG 1 cut(s) 550
BtrI CACGTC 1 cut(s) 958
BtsCI GGATG 2 cut(s) 107, 152
BtsIMutI CAGTG 1 cut(s) 8
Cac8I GCNNGC 6 cut(s) 298, 464, 672, 710, 739, 976
CaiI CAGNNNCTG 1 cut(s) 883
CfoI GCGC 5 cut(s) 380, 578, 737, 785, 944
Cfr10I RCCGGY 1 cut(s) 296
Cfr42I CCGCGG 1 cut(s) 553
Cfr9I CCCGGG 1 cut(s) 760
Csp6I GTAC 2 cut(s) 748, 1036
CviAII CATG 5 cut(s) 331, 475, 637, 904, 1013
CviQI GTAC 2 cut(s) 748, 1036
DdeI CTNAG 3 cut(s) 266, 731, 884
DpnI GATC 8 cut(s) 165, 293, 407, 456, 635, 683, 729, 983
DpnII GATC 8 cut(s) 163, 291, 405, 454, 633, 681, 727, 981
DraIII CACNNNGTG 1 cut(s) 907
DrdI GACNNNNNNGTC 1 cut(s) 320
DseDI GACNNNNNNGTC 1 cut(s) 320
EaeI YGGCCR 2 cut(s) 3, 687
EagI CGGCCG 1 cut(s) 687
Eam1104I CTCTTC 2 cut(s) 58, 263
EarI CTCTTC 2 cut(s) 58, 263
EciI GGCGGA 3 cut(s) 13, 363, 547
EclXI CGGCCG 1 cut(s) 687
Eco130I CCWWGG 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 876
Eco47I GGWCC 4 cut(s) 622, 757, 775, 880
Eco52I CGGCCG 1 cut(s) 687
Eco72I CACGTG 1 cut(s) 893
Eco81I CCTNAGG 1 cut(s) 884
Eco88I CYCGRG 1 cut(s) 760
EcoO109I RGGNCCY 4 cut(s) 757, 837, 873, 880
EcoRII CCWGG 3 cut(s) 434, 642, 876
EcoT14I CCWWGG 1 cut(s) 498
EcoT38I GRGCYC 1 cut(s) 876
ErhI CCWWGG 1 cut(s) 498
Esp3I CGTCTC 1 cut(s) 523
FaeI CATG 5 cut(s) 334, 478, 640, 907, 1016
FaiI YATR 6 cut(s) 332, 476, 638, 860, 905, 1014
FaqI GGGAC 3 cut(s) 45, 208, 770
FatI CATG 5 cut(s) 330, 474, 636, 903, 1012
FauI CCCGC 4 cut(s) 216, 233, 471, 547
FbaI TGATCA 2 cut(s) 633, 981
FblI GTMKAC 2 cut(s) 327, 921
Fnu4HI GCNGC 6 cut(s) 387, 416, 529, 553, 687, 945
FokI GGATG 2 cut(s) 94, 139
FriOI GRGCYC 1 cut(s) 876
Fsp4HI GCNGC 6 cut(s) 387, 416, 529, 553, 687, 945
FspBI CTAG 2 cut(s) 57, 452
GlaI GCGC 5 cut(s) 379, 577, 736, 784, 943
GluI GCNGC 6 cut(s) 387, 416, 529, 553, 687, 945
GsaI CCCAGC 2 cut(s) 815, 852
GsuI CTGGAG 1 cut(s) 565
HaeII RGCGCY 1 cut(s) 738
HhaI GCGC 5 cut(s) 380, 578, 737, 785, 944
Hin1I GRCGYC 1 cut(s) 924
Hin1II CATG 5 cut(s) 334, 478, 640, 907, 1016
Hin6I GCGC 5 cut(s) 378, 576, 735, 783, 942
HinP1I GCGC 5 cut(s) 378, 576, 735, 783, 942
HincII GTYRAC 4 cut(s) 184, 328, 703, 922
HindII GTYRAC 4 cut(s) 184, 328, 703, 922
HinfI GANTC 4 cut(s) 13, 351, 422, 546
HphI GGTGA 3 cut(s) 24, 118, 1028
Hpy166II GTNNAC 4 cut(s) 184, 328, 703, 922
Hpy188I TCNGA 5 cut(s) 41, 163, 358, 732, 994
Hpy188III TCNNGA 2 cut(s) 481, 778
Hpy8I GTNNAC 4 cut(s) 184, 328, 703, 922
Hpy99I CGWCG 9 cut(s) 323, 326, 329, 572, 599, 695, 923, 926, 929
HpyAV CCTTC 4 cut(s) 689, 766, 784, 796
HpyCH4III ACNGT 2 cut(s) 181, 568
HpyCH4IV ACGT 3 cut(s) 892, 924, 957
HpyCH4V TGCA 2 cut(s) 712, 1025
HpyF3I CTNAG 3 cut(s) 266, 731, 884
HpySE526I ACGT 3 cut(s) 892, 924, 957
Hsp92I GRCGYC 1 cut(s) 924
Hsp92II CATG 5 cut(s) 334, 478, 640, 907, 1016
HspAI GCGC 5 cut(s) 378, 576, 735, 783, 942
KflI GGGWCCC 1 cut(s) 757
Kpn2I TCCGGA 1 cut(s) 777
KpnI GGTACC 1 cut(s) 751
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
Ksp22I TGATCA 2 cut(s) 633, 981
KspI CCGCGG 1 cut(s) 553
Kzo9I GATC 8 cut(s) 163, 291, 405, 454, 633, 681, 727, 981
LmnI GCTCC 7 cut(s) 334, 500, 560, 780, 939, 1009, 1041
Lsp1109I GCAGC 2 cut(s) 402, 956
LweI GCATC 2 cut(s) 600, 1012
MaeI CTAG 2 cut(s) 57, 452
MaeII ACGT 3 cut(s) 892, 924, 957
MalI GATC 8 cut(s) 165, 293, 407, 456, 635, 683, 729, 983
MbiI CCGCTC 1 cut(s) 674
MboI GATC 8 cut(s) 163, 291, 405, 454, 633, 681, 727, 981
MboII GAAGA 2 cut(s) 45, 250
MfeI CAATTG 1 cut(s) 626
MflI RGATCY 2 cut(s) 454, 727
MhlI GDGCHC 3 cut(s) 339, 818, 876
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 613, 626
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 705
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 777
MroNI GCCGGC 1 cut(s) 296
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 1017
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 552, 947
MspR9I CCNGG 7 cut(s) 345, 401, 436, 644, 761, 762, 878
MunI CAATTG 1 cut(s) 626
MvaI CCWGG 3 cut(s) 436, 644, 878
MvnI CGCG 6 cut(s) 378, 431, 552, 576, 686, 785
NaeI GCCGGC 1 cut(s) 298
NciI CCSGG 4 cut(s) 345, 401, 761, 762
NdeII GATC 8 cut(s) 163, 291, 405, 454, 633, 681, 727, 981
NgoMIV GCCGGC 1 cut(s) 296
NlaIII CATG 5 cut(s) 334, 478, 640, 907, 1016
NmeAIII GCCGAG 3 cut(s) 313, 438, 823
PcsI WCGNNNNNNNCGW 4 cut(s) 318, 324, 594, 690
PdiI GCCGGC 1 cut(s) 298
PfeI GAWTC 3 cut(s) 351, 422, 546
PflFI GACNNNGTC 1 cut(s) 317
PkrI GCNGC 6 cut(s) 388, 417, 530, 554, 688, 946
PleI GAGTC 1 cut(s) 7
PmaCI CACGTG 1 cut(s) 893
PmlI CACGTG 1 cut(s) 893
PpsI GAGTC 1 cut(s) 7
Ppu21I YACGTR 1 cut(s) 893
PpuMI RGGWCCY 2 cut(s) 757, 880
Psp5II RGGWCCY 2 cut(s) 757, 880
Psp6I CCWGG 3 cut(s) 434, 642, 876
PspCI CACGTG 1 cut(s) 893
PspFI CCCAGC 2 cut(s) 811, 848
PspGI CCWGG 3 cut(s) 434, 642, 876
PspOMI GGGCCC 1 cut(s) 872
PspPPI RGGWCCY 2 cut(s) 757, 880
PstNI CAGNNNCTG 1 cut(s) 883
PsuI RGATCY 2 cut(s) 454, 727
PsyI GACNNNGTC 1 cut(s) 317
RsaI GTAC 2 cut(s) 749, 1037
RsaNI GTAC 2 cut(s) 748, 1036
RseI CAYNNNNRTG 1 cut(s) 1017
SacII CCGCGG 1 cut(s) 553
SalI GTCGAC 2 cut(s) 326, 920
SatI GCNGC 6 cut(s) 387, 416, 529, 553, 687, 945
Sau3AI GATC 8 cut(s) 163, 291, 405, 454, 633, 681, 727, 981
ScaI AGTACT 1 cut(s) 1037
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 7 cut(s) 345, 401, 436, 644, 761, 762, 878
SduI GDGCHC 3 cut(s) 339, 818, 876
SfaNI GCATC 2 cut(s) 600, 1012
Sfr303I CCGCGG 1 cut(s) 553
SgrBI CCGCGG 1 cut(s) 553
SgrDI CGTCGACG 1 cut(s) 920
SinI GGWCC 4 cut(s) 622, 757, 775, 880
SmaI CCCGGG 1 cut(s) 762
SmiMI CAYNNNNRTG 1 cut(s) 1017
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 2 cut(s) 613, 626
SspMI CTAG 2 cut(s) 57, 452
StyD4I CCNGG 7 cut(s) 343, 399, 434, 642, 759, 760, 876
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 2 cut(s) 181, 568
TaiI ACGT 3 cut(s) 895, 927, 960
TaqI TCGA 4 cut(s) 327, 594, 693, 921
TasI AATT 2 cut(s) 613, 626
TatI WGTACW 1 cut(s) 1035
TauI GCSGC 4 cut(s) 389, 531, 555, 689
TfiI GAWTC 3 cut(s) 351, 422, 546
TscAI CASTG 1 cut(s) 15
TseI GCWGC 2 cut(s) 415, 944
TspGWI ACGGA 1 cut(s) 907
TspMI CCCGGG 1 cut(s) 760
TspRI CASTG 1 cut(s) 15
Tth111I GACNNNGTC 1 cut(s) 317
VpaK11BI GGWCC 4 cut(s) 622, 757, 775, 880
XmaI CCCGGG 1 cut(s) 760
XmiI GTMKAC 2 cut(s) 327, 921
XspI CTAG 2 cut(s) 57, 452
ZraI GACGTC 1 cut(s) 925
ZrmI AGTACT 1 cut(s) 1037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.