Rmu_sc0009734.1_g000001

HpcH/HpaI aldolase/citrate lyase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009734.1
OG0012383
Physical Location & Seq
Forward (+)
185 .. 1246
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009734.1_g000001.1.cds

Sequence Viewer

Length: 1062 bp
atggccacacagacgcacgccgccgccgccatcgccatcaccgcctcatcaccgctcgcaaaacccatctcttccagtcccaccaccaccaatcccctatcccatctctctttccctttctccccatccaaaaccctaaacctcaccgccaaaaccacctcaatcaaatcctcctcctcctccgatctctccaccaccaccgtaacgaccacctccatctcctcctccccgcaaaccctcaagtcccgcctccacaatggcgacaccctctacggcctcttcctcctcagcttctcccccaccctcgccgagatcgccggcctctccggctacgacttcgtcgtcgtcgacatggagcacggccccggcggaatctccgaagccctctcctgcctccgcgccctcgccgccacccaaaccccggcgatcctccggctgccggaatcctccccgacctgggccaagaaagccctcgatctcggcccgcaggggatcatgttcccaatgatcgacagctccaaggaggccgagaaagccgtctcctactgccgcttccctcccgccggcatccgcggctccgcacacaccgtcgtccgagcctccagctacggcatcgacgaggggtatttgagtaattacgaggaccaattgctgatcatgtgccaggtggagaccgaggagggcgtgaagagagcggaggagatcgcggccgtcgaaggcgttgactgcgtgcagatggggccgttggatctgagcgccagcctggggtacctgtgggacccggggaacaagaaggtccggaacgcgttgaaggttgccgagaaggccgtactgggcaccgacccccttgtgggtggggcctacttggctgggttcgctatgccccacgatgggccccaggacctgaggacacgtggctaccacatggtgtccggttccgtcgacgtcgggctgtttaggagcgcagcagtggaggacgtgaggaggtttcaggcgagcttgatcaacggctctgatgaggagcgtggggatgatgcagatgagaagtactggagcgaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HpcH_HpaI

Protein Analysis

353

Amino Acids

37.43

Weight (kDa)

5.16

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10750
fragaria_vesca FvH4_5g11021 FvH4_5g11021
malus_domestica MD06G1117400.v1.1
prunus_persica Prupe.5G128500_v2.0.a1
pyrus_communis pycom06g10930
rosa_chinensis RchiOBHm_Chr7g0186781
rosa_laevigata RLG00000004816
rosa_multiflora Rmu_co8215596.1_g000001 Rmu_sc0009734.1_g000001 Rmu_sc0040245.1_g000001 Rmu_ssc0000024.1_g000045
rosa_roxburghii Rroxscaffold_3G00267830
rosa_rugosa Rorug06G0476600
rosa_samantha Rh7AG063900 Rh7BG080400 Rh7CG083600 Rh7DG084500
rosa_wichuraiana Rw7G007250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 341
AatII GACGTC 1 cut(s) 948
Acc65I GGTACC 1 cut(s) 768
AccB1I GGYRCC 2 cut(s) 768, 836
AccBSI CCGCTC 2 cut(s) 55, 695
AccI GTMKAC 2 cut(s) 348, 942
AccII CGCG 4 cut(s) 399, 573, 707, 806
AccIII TCCGGA 1 cut(s) 798
AclWI GGATC 3 cut(s) 421, 500, 756
AcoI YGGCCR 2 cut(s) 3, 708
AcvI CACGTG 1 cut(s) 914
AcyI GRCGYC 1 cut(s) 945
AdeI CACNNNGTG 1 cut(s) 928
AfaI GTAC 3 cut(s) 770, 831, 1049
AflIII ACRYGT 2 cut(s) 804, 911
AgsI TTSAA 1 cut(s) 811
AjiI CACGTC 1 cut(s) 979
AjnI CCWGG 4 cut(s) 455, 663, 762, 897
AluBI AGCT 4 cut(s) 291, 516, 606, 999
AluI AGCT 4 cut(s) 291, 516, 606, 999
Alw21I GWGCWC 1 cut(s) 360
Alw26I GTCTC 2 cut(s) 544, 665
AlwI GGATC 3 cut(s) 421, 500, 756
AlwNI CAGNNNCTG 1 cut(s) 904
Ama87I CYCGRG 1 cut(s) 781
Aor13HI TCCGGA 1 cut(s) 798
ApaI GGGCCC 1 cut(s) 897
ApeKI GCWGC 2 cut(s) 436, 965
Asp718I GGTACC 1 cut(s) 768
AspLEI GCGC 3 cut(s) 401, 758, 965
AsuC2I CCSGG 4 cut(s) 366, 422, 782, 783
AsuHPI GGTGA 3 cut(s) 31, 42, 136
AvaI CYCGRG 1 cut(s) 781
AvaII GGWCC 4 cut(s) 643, 778, 796, 901
AxyI CCTNAGG 1 cut(s) 905
BaeGI GKGCMC 2 cut(s) 839, 897
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 768, 836
BanII GRGCYC 1 cut(s) 897
BbrPI CACGTG 1 cut(s) 914
Bbv12I GWGCWC 1 cut(s) 360
BbvCI CCTCAGC 1 cut(s) 287
BbvI GCAGC 2 cut(s) 423, 977
BccI CCATC 8 cut(s) 38, 44, 74, 111, 133, 224, 730, 884
BceAI ACGGC 8 cut(s) 289, 376, 521, 625, 695, 727, 812, 1024
BciT130I CCWGG 4 cut(s) 457, 665, 764, 899
BclI TGATCA 2 cut(s) 654, 1002
BcnI CCSGG 4 cut(s) 366, 422, 782, 783
BcoDI GTCTC 2 cut(s) 544, 665
BfoI RGCGCY 1 cut(s) 759
BglI GCCNNNNNGGC 3 cut(s) 327, 824, 866
BisI GCNGC 9 cut(s) 21, 24, 27, 408, 437, 550, 574, 708, 966
BlsI GCNGC 9 cut(s) 22, 25, 28, 409, 438, 551, 575, 709, 967
BmcAI AGTACT 1 cut(s) 1049
Bme1390I CCNGG 8 cut(s) 366, 422, 457, 665, 764, 782, 783, 899
Bme18I GGWCC 4 cut(s) 643, 778, 796, 901
BmeT110I CYCGRG 1 cut(s) 781
BmgBI CACGTC 1 cut(s) 979
BmrFI CCNGG 8 cut(s) 366, 422, 457, 665, 764, 782, 783, 899
BmrI ACTGGG 1 cut(s) 842
BmsI GCATC 3 cut(s) 576, 621, 1024
BmuI ACTGGG 1 cut(s) 842
BpmI CTGGAG 1 cut(s) 586
Bpu10I CCTNAGC 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 4 cut(s) 366, 422, 782, 783
BsaAI YACGTR 1 cut(s) 914
BsaHI GRCGYC 1 cut(s) 945
BsaI GGTCTC 1 cut(s) 665
BsaWI WCCGGW 2 cut(s) 798, 932
Bse118I RCCGGY 2 cut(s) 317, 563
Bse1I ACTGG 3 cut(s) 75, 837, 1055
Bse21I CCTNAGG 1 cut(s) 905
BseAI TCCGGA 1 cut(s) 798
BseBI CCWGG 4 cut(s) 457, 665, 764, 899
BseGI GGATG 3 cut(s) 125, 567, 1036
BseMII CTCAG 3 cut(s) 301, 743, 896
BseNI ACTGG 3 cut(s) 75, 837, 1055
BseSI GKGCMC 2 cut(s) 839, 897
BseX3I CGGCCG 1 cut(s) 708
BseXI GCAGC 2 cut(s) 423, 977
BseYI CCCAGC 1 cut(s) 869
BsgI GTGCAG 1 cut(s) 752
Bsh1236I CGCG 4 cut(s) 399, 573, 707, 806
Bsh1285I CGRYCG 1 cut(s) 711
BshNI GGYRCC 2 cut(s) 768, 836
BsiEI CGRYCG 1 cut(s) 711
BsiHKAI GWGCWC 1 cut(s) 360
BsiHKCI CYCGRG 1 cut(s) 781
BslFI GGGAC 3 cut(s) 63, 229, 791
BsmAI GTCTC 2 cut(s) 544, 665
BsmBI CGTCTC 1 cut(s) 544
BsmFI GGGAC 3 cut(s) 63, 229, 791
Bso31I GGTCTC 1 cut(s) 665
BsoBI CYCGRG 1 cut(s) 781
Bsp120I GGGCCC 1 cut(s) 893
Bsp1286I GDGCHC 3 cut(s) 360, 839, 897
Bsp13I TCCGGA 1 cut(s) 798
BspCNI CTCAG 3 cut(s) 300, 744, 897
BspEI TCCGGA 1 cut(s) 798
BspFNI CGCG 4 cut(s) 399, 573, 707, 806
BspPI GGATC 3 cut(s) 421, 500, 756
BspT107I GGYRCC 2 cut(s) 768, 836
BspTNI GGTCTC 1 cut(s) 665
BsrBI CCGCTC 2 cut(s) 55, 695
BsrFI RCCGGY 2 cut(s) 317, 563
BsrI ACTGG 3 cut(s) 75, 837, 1055
BssAI RCCGGY 2 cut(s) 317, 563
BssNI GRCGYC 1 cut(s) 945
BssT1I CCWWGG 1 cut(s) 519
Bst2UI CCWGG 4 cut(s) 457, 665, 764, 899
Bst4CI ACNGT 2 cut(s) 202, 589
Bst6I CTCTTC 3 cut(s) 76, 284, 683
BstACI GRCGYC 1 cut(s) 945
BstBAI YACGTR 1 cut(s) 914
BstC8I GCNNGC 8 cut(s) 18, 57, 319, 485, 565, 731, 760, 997
BstDEI CTNAG 3 cut(s) 287, 752, 905
BstDSI CCRYGG 1 cut(s) 571
BstF5I GGATG 3 cut(s) 125, 567, 1036
BstFNI CGCG 4 cut(s) 399, 573, 707, 806
BstH2I RGCGCY 1 cut(s) 759
BstHHI GCGC 3 cut(s) 401, 758, 965
BstMAI GTCTC 2 cut(s) 544, 665
BstMCI CGRYCG 1 cut(s) 711
BstNI CCWGG 4 cut(s) 457, 665, 764, 899
BstSCI CCNGG 8 cut(s) 364, 420, 455, 663, 762, 780, 781, 897
BstSLI GKGCMC 2 cut(s) 839, 897
BstUI CGCG 4 cut(s) 399, 573, 707, 806
BstV1I GCAGC 2 cut(s) 423, 977
BstX2I RGATCY 1 cut(s) 748
BstYI RGATCY 1 cut(s) 748
BstZI CGGCCG 1 cut(s) 708
Bsu36I CCTNAGG 1 cut(s) 905
BtgI CCRYGG 1 cut(s) 571
BtgZI GCGATG 1 cut(s) 16
BtrI CACGTC 1 cut(s) 979
BtsCI GGATG 3 cut(s) 125, 567, 1036
BtsI GCAGTG 1 cut(s) 975
BtsIMutI CAGTG 1 cut(s) 975
Cac8I GCNNGC 8 cut(s) 18, 57, 319, 485, 565, 731, 760, 997
CaiI CAGNNNCTG 1 cut(s) 904
CfoI GCGC 3 cut(s) 401, 758, 965
Cfr10I RCCGGY 2 cut(s) 317, 563
Cfr42I CCGCGG 1 cut(s) 574
Cfr9I CCCGGG 1 cut(s) 781
CseI GACGC 1 cut(s) 22
Csp6I GTAC 3 cut(s) 769, 830, 1048
CviAII CATG 4 cut(s) 352, 496, 658, 925
CviQI GTAC 3 cut(s) 769, 830, 1048
DdeI CTNAG 3 cut(s) 287, 752, 905
DraIII CACNNNGTG 1 cut(s) 928
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
EaeI YGGCCR 2 cut(s) 3, 708
EagI CGGCCG 1 cut(s) 708
Eam1104I CTCTTC 3 cut(s) 76, 284, 683
EarI CTCTTC 3 cut(s) 76, 284, 683
EciI GGCGGA 1 cut(s) 384
EclXI CGGCCG 1 cut(s) 708
Eco130I CCWWGG 1 cut(s) 519
Eco24I GRGCYC 1 cut(s) 897
Eco31I GGTCTC 1 cut(s) 665
Eco47I GGWCC 4 cut(s) 643, 778, 796, 901
Eco52I CGGCCG 1 cut(s) 708
Eco72I CACGTG 1 cut(s) 914
Eco81I CCTNAGG 1 cut(s) 905
Eco88I CYCGRG 1 cut(s) 781
EcoO109I RGGNCCY 4 cut(s) 778, 858, 894, 901
EcoRII CCWGG 4 cut(s) 455, 663, 762, 897
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 1 cut(s) 897
ErhI CCWWGG 1 cut(s) 519
Esp3I CGTCTC 1 cut(s) 544
FaeI CATG 4 cut(s) 355, 499, 661, 928
FaiI YATR 5 cut(s) 353, 497, 659, 881, 926
FaqI GGGAC 3 cut(s) 63, 229, 791
FatI CATG 4 cut(s) 351, 495, 657, 924
FauI CCCGC 4 cut(s) 237, 254, 492, 568
FbaI TGATCA 2 cut(s) 654, 1002
FblI GTMKAC 2 cut(s) 348, 942
Fnu4HI GCNGC 9 cut(s) 21, 24, 27, 408, 437, 550, 574, 708, 966
FokI GGATG 3 cut(s) 112, 554, 1043
FriOI GRGCYC 1 cut(s) 897
Fsp4HI GCNGC 9 cut(s) 21, 24, 27, 408, 437, 550, 574, 708, 966
GlaI GCGC 3 cut(s) 400, 757, 964
GluI GCNGC 9 cut(s) 21, 24, 27, 408, 437, 550, 574, 708, 966
GsaI CCCAGC 1 cut(s) 873
GsuI CTGGAG 1 cut(s) 586
HaeII RGCGCY 1 cut(s) 759
HgaI GACGC 1 cut(s) 22
HhaI GCGC 3 cut(s) 401, 758, 965
Hin1I GRCGYC 1 cut(s) 945
Hin1II CATG 4 cut(s) 355, 499, 661, 928
Hin6I GCGC 3 cut(s) 399, 756, 963
HinP1I GCGC 3 cut(s) 399, 756, 963
HincII GTYRAC 3 cut(s) 349, 724, 943
HindII GTYRAC 3 cut(s) 349, 724, 943
HinfI GANTC 2 cut(s) 372, 443
HphI GGTGA 3 cut(s) 31, 42, 136
Hpy166II GTNNAC 3 cut(s) 349, 724, 943
Hpy188I TCNGA 5 cut(s) 184, 379, 596, 753, 1015
Hpy188III TCNNGA 1 cut(s) 799
Hpy8I GTNNAC 3 cut(s) 349, 724, 943
Hpy99I CGWCG 9 cut(s) 344, 347, 350, 593, 620, 716, 944, 947, 950
HpyAV CCTTC 4 cut(s) 710, 787, 805, 817
HpyCH4III ACNGT 2 cut(s) 202, 589
HpyCH4IV ACGT 3 cut(s) 913, 945, 978
HpyCH4V TGCA 2 cut(s) 733, 1037
HpyF3I CTNAG 3 cut(s) 287, 752, 905
HpySE526I ACGT 3 cut(s) 913, 945, 978
Hsp92I GRCGYC 1 cut(s) 945
Hsp92II CATG 4 cut(s) 355, 499, 661, 928
HspAI GCGC 3 cut(s) 399, 756, 963
KflI GGGWCCC 1 cut(s) 778
Kpn2I TCCGGA 1 cut(s) 798
KpnI GGTACC 1 cut(s) 772
KroI GCCGGC 2 cut(s) 317, 563
KroNI GCCGGC 2 cut(s) 319, 565
Ksp22I TGATCA 2 cut(s) 654, 1002
KspI CCGCGG 1 cut(s) 574
LmnI GCTCC 6 cut(s) 355, 521, 581, 960, 1021, 1053
Lsp1109I GCAGC 2 cut(s) 423, 977
LweI GCATC 3 cut(s) 576, 621, 1024
MaeII ACGT 3 cut(s) 913, 945, 978
MaeIII GTNAC 1 cut(s) 202
MbiI CCGCTC 2 cut(s) 55, 695
MboII GAAGA 3 cut(s) 63, 271, 700
MfeI CAATTG 1 cut(s) 647
MflI RGATCY 1 cut(s) 748
MhlI GDGCHC 3 cut(s) 360, 839, 897
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 634, 647
MluI ACGCGT 1 cut(s) 804
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 726
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 798
MroNI GCCGGC 2 cut(s) 317, 563
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 573
MspR9I CCNGG 8 cut(s) 366, 422, 457, 665, 764, 782, 783, 899
MunI CAATTG 1 cut(s) 647
MvaI CCWGG 4 cut(s) 457, 665, 764, 899
MvnI CGCG 4 cut(s) 399, 573, 707, 806
NaeI GCCGGC 2 cut(s) 319, 565
NciI CCSGG 4 cut(s) 366, 422, 782, 783
NgoMIV GCCGGC 2 cut(s) 317, 563
NlaIII CATG 4 cut(s) 355, 499, 661, 928
NmeAIII GCCGAG 4 cut(s) 334, 459, 553, 844
PcsI WCGNNNNNNNCGW 4 cut(s) 339, 345, 615, 711
PdiI GCCGGC 2 cut(s) 319, 565
PfeI GAWTC 2 cut(s) 372, 443
PflFI GACNNNGTC 1 cut(s) 338
PkrI GCNGC 9 cut(s) 22, 25, 28, 409, 438, 551, 575, 709, 967
PmaCI CACGTG 1 cut(s) 914
PmlI CACGTG 1 cut(s) 914
Ppu21I YACGTR 1 cut(s) 914
PpuMI RGGWCCY 2 cut(s) 778, 901
Psp5II RGGWCCY 2 cut(s) 778, 901
Psp6I CCWGG 4 cut(s) 455, 663, 762, 897
PspCI CACGTG 1 cut(s) 914
PspFI CCCAGC 1 cut(s) 869
PspGI CCWGG 4 cut(s) 455, 663, 762, 897
PspOMI GGGCCC 1 cut(s) 893
PspPPI RGGWCCY 2 cut(s) 778, 901
PstNI CAGNNNCTG 1 cut(s) 904
PsuI RGATCY 1 cut(s) 748
PsyI GACNNNGTC 1 cut(s) 338
RsaI GTAC 3 cut(s) 770, 831, 1049
RsaNI GTAC 3 cut(s) 769, 830, 1048
SacII CCGCGG 1 cut(s) 574
SalI GTCGAC 2 cut(s) 347, 941
SatI GCNGC 9 cut(s) 21, 24, 27, 408, 437, 550, 574, 708, 966
ScaI AGTACT 1 cut(s) 1049
ScrFI CCNGG 8 cut(s) 366, 422, 457, 665, 764, 782, 783, 899
SduI GDGCHC 3 cut(s) 360, 839, 897
SfaNI GCATC 3 cut(s) 576, 621, 1024
Sfr303I CCGCGG 1 cut(s) 574
SgrBI CCGCGG 1 cut(s) 574
SgrDI CGTCGACG 1 cut(s) 941
SinI GGWCC 4 cut(s) 643, 778, 796, 901
SmaI CCCGGG 1 cut(s) 783
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 2 cut(s) 634, 647
StyD4I CCNGG 8 cut(s) 364, 420, 455, 663, 762, 780, 781, 897
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 2 cut(s) 202, 589
TaiI ACGT 3 cut(s) 916, 948, 981
TaqI TCGA 6 cut(s) 348, 474, 510, 615, 714, 942
TaqII GACCGA 1 cut(s) 689
TasI AATT 2 cut(s) 634, 647
TatI WGTACW 1 cut(s) 1047
TauI GCSGC 7 cut(s) 23, 26, 29, 410, 552, 576, 710
TfiI GAWTC 2 cut(s) 372, 443
TscAI CASTG 1 cut(s) 975
TseI GCWGC 2 cut(s) 436, 965
TspGWI ACGGA 1 cut(s) 928
TspMI CCCGGG 1 cut(s) 781
TspRI CASTG 1 cut(s) 975
Tth111I GACNNNGTC 1 cut(s) 338
VpaK11BI GGWCC 4 cut(s) 643, 778, 796, 901
XmaI CCCGGG 1 cut(s) 781
XmiI GTMKAC 2 cut(s) 348, 942
ZraI GACGTC 1 cut(s) 946
ZrmI AGTACT 1 cut(s) 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.