Rroxscaffold_3G00267830

HpcH/HpaI aldolase/citrate lyase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012383
Physical Location & Seq
Forward (+)
60993273 .. 60994337
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00267830.1

Sequence Viewer

Length: 1065 bp
ATGGCCACACTGACTCACGCCGCCGCCGCCGCCGCCATCACCGCCTCATCACCTCTCACAAAACCCATCTCTTCCAGTCCCACCACCACCAACCCCCTATCCCATCTCTCTTTCCCTTTCTCCCCATCCAAAACCCTAAACCTCACCACCAAAACCACCTCAATCAAATCCTCCTCCGATCTCTCCTCCATCACCGTCACCACCACCACCACCTCCTCCTCCCCGCAAACCCTCAAGTCCCGCCTCCACAATGGCGACACCCTCTACGGCCTCTTCCTCCTCAGCTTCTCCCCCACCCTCGCCGAGATCGCCGGCCTCTCCGGCTACGACTTCGTCGTCGTCGACATGGAGCACGGCCCCGGCGGCATCTCCGAGGCCCTCTCCTGCCTCCGCGCCCTCGCCGCCACCCAAACCCCGGCGATTCTCCGGCTGCCGGAATCCTCCCCGACCTGGGCCAAGAAAGCCCTAGATCTCGGCCCGCAGGGGATCATGTTCCCGATGATCGACAGCTCCAAGGAGGCCGAGAAAGCCGTCTCCTACTGCCGCTTCCCTCCCGCCGGCGTCCGCGGCTCCGCCCACACCGTCGTCCGCGCCTCCAGCTACGGCATCGACGAGGGGTATTTGGGTAATTACGAGGACCAATTGCTGATCATGTGCCAGGTGGAGAGCGAGGAGGGCGTGAAGAGAGCGGAGGAGATCGCGGCCGTCGAAGGCGTTGACTGCGTGCAGATGGGGCCGTTGGATCTGAGCGCCAGCTTGGGGTACCTGTGGGACCCGGGGAACAGGAAGGTCCGGAACGCGTTGAAGGCTGCCGAGAAGGCCGTGCTGGGCACCGACCCCCTTGTGGGTGGGGCCTACTTGGCTGGGTTCGCTATGCCCCACGATGGGCCCCAGGACCTGAGGACACGTGGCTACCACATGGTGTCCGGTTCCGTCGACGTCGGGCTGTTTAGGAGCGCAGCGGTGGAGGACGTGAGGAGGTTTCAGGCGAGCTTGATTAACGGCTCTGATGAGGAGGAGGAGGAGCATGGTGATGATGCAGATGAGAAGTACTGGAGCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HpcH_HpaI

Protein Analysis

354

Amino Acids

37.57

Weight (kDa)

4.98

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HpcH_HpaI PF03328 89 - 319 1e-54 HpcH/HpaI aldolase/citrate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10750
fragaria_vesca FvH4_5g11021 FvH4_5g11021
malus_domestica MD06G1117400.v1.1
prunus_persica Prupe.5G128500_v2.0.a1
pyrus_communis pycom06g10930
rosa_chinensis RchiOBHm_Chr7g0186781
rosa_laevigata RLG00000004816
rosa_multiflora Rmu_co8215596.1_g000001 Rmu_sc0009734.1_g000001 Rmu_sc0040245.1_g000001 Rmu_ssc0000024.1_g000045
rosa_roxburghii Rroxscaffold_3G00267830
rosa_rugosa Rorug06G0476600
rosa_samantha Rh7AG063900 Rh7BG080400 Rh7CG083600 Rh7DG084500
rosa_wichuraiana Rw7G007250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 335
AatII GACGTC 1 cut(s) 942
Acc65I GGTACC 1 cut(s) 762
AccB1I GGYRCC 2 cut(s) 762, 830
AccBSI CCGCTC 1 cut(s) 689
AccI GTMKAC 2 cut(s) 342, 936
AccII CGCG 5 cut(s) 393, 567, 591, 701, 800
AccIII TCCGGA 1 cut(s) 792
AclWI GGATC 2 cut(s) 494, 750
AcoI YGGCCR 2 cut(s) 3, 702
AcvI CACGTG 1 cut(s) 908
AcyI GRCGYC 2 cut(s) 561, 939
AdeI CACNNNGTG 1 cut(s) 922
AfaI GTAC 2 cut(s) 764, 1052
AflIII ACRYGT 2 cut(s) 798, 905
AgsI TTSAA 1 cut(s) 805
AjiI CACGTC 1 cut(s) 973
AjnI CCWGG 3 cut(s) 449, 657, 891
AluBI AGCT 5 cut(s) 285, 510, 600, 756, 993
AluI AGCT 5 cut(s) 285, 510, 600, 756, 993
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 1 cut(s) 538
AlwI GGATC 2 cut(s) 494, 750
AlwNI CAGNNNCTG 1 cut(s) 898
Ama87I CYCGRG 1 cut(s) 775
Aor13HI TCCGGA 1 cut(s) 792
ApaI GGGCCC 1 cut(s) 891
ApeKI GCWGC 3 cut(s) 430, 809, 959
Asp718I GGTACC 1 cut(s) 762
AspLEI GCGC 4 cut(s) 395, 593, 752, 959
AsuC2I CCSGG 4 cut(s) 360, 416, 776, 777
AsuHPI GGTGA 6 cut(s) 31, 42, 136, 184, 190, 1043
AvaI CYCGRG 1 cut(s) 775
AvaII GGWCC 4 cut(s) 637, 772, 790, 895
AxyI CCTNAGG 1 cut(s) 899
BaeGI GKGCMC 2 cut(s) 833, 891
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 2 cut(s) 762, 830
BanII GRGCYC 1 cut(s) 891
BbrPI CACGTG 1 cut(s) 908
Bbv12I GWGCWC 1 cut(s) 354
BbvCI CCTCAGC 1 cut(s) 281
BbvI GCAGC 3 cut(s) 417, 796, 971
BccI CCATC 7 cut(s) 44, 74, 111, 133, 197, 724, 878
BceAI ACGGC 8 cut(s) 283, 370, 515, 619, 689, 721, 806, 1018
BciT130I CCWGG 3 cut(s) 451, 659, 893
BclI TGATCA 1 cut(s) 648
BcnI CCSGG 4 cut(s) 360, 416, 776, 777
BcoDI GTCTC 1 cut(s) 538
BfaI CTAG 1 cut(s) 467
BfoI RGCGCY 1 cut(s) 753
BglI GCCNNNNNGGC 4 cut(s) 321, 363, 818, 860
BglII AGATCT 1 cut(s) 469
BmcAI AGTACT 1 cut(s) 1052
Bme1390I CCNGG 7 cut(s) 360, 416, 451, 659, 776, 777, 893
Bme18I GGWCC 4 cut(s) 637, 772, 790, 895
BmeT110I CYCGRG 1 cut(s) 775
BmgBI CACGTC 1 cut(s) 973
BmrFI CCNGG 7 cut(s) 360, 416, 451, 659, 776, 777, 893
BmsI GCATC 3 cut(s) 375, 615, 1027
BplI GAGNNNNNCTC 2 cut(s) 365, 397
BpmI CTGGAG 1 cut(s) 580
Bpu10I CCTNAGC 1 cut(s) 281
BpuEI CTTGAG 1 cut(s) 218
BpuMI CCSGG 4 cut(s) 360, 416, 776, 777
BsaAI YACGTR 1 cut(s) 908
BsaHI GRCGYC 2 cut(s) 561, 939
BsaJI CCNNGG 9 cut(s) 358, 372, 414, 450, 513, 565, 775, 776, 891
BsaWI WCCGGW 2 cut(s) 792, 926
Bse118I RCCGGY 2 cut(s) 311, 557
Bse1I ACTGG 2 cut(s) 75, 1058
Bse21I CCTNAGG 1 cut(s) 899
BseAI TCCGGA 1 cut(s) 792
BseBI CCWGG 3 cut(s) 451, 659, 893
BseDI CCNNGG 9 cut(s) 358, 372, 414, 450, 513, 565, 775, 776, 891
BseGI GGATG 1 cut(s) 125
BseMII CTCAG 3 cut(s) 295, 737, 890
BseNI ACTGG 2 cut(s) 75, 1058
BseSI GKGCMC 2 cut(s) 833, 891
BseX3I CGGCCG 1 cut(s) 702
BseXI GCAGC 3 cut(s) 417, 796, 971
BseYI CCCAGC 2 cut(s) 826, 863
BsgI GTGCAG 1 cut(s) 746
Bsh1236I CGCG 5 cut(s) 393, 567, 591, 701, 800
Bsh1285I CGRYCG 1 cut(s) 705
BshNI GGYRCC 2 cut(s) 762, 830
BsiEI CGRYCG 1 cut(s) 705
BsiHKAI GWGCWC 1 cut(s) 354
BsiHKCI CYCGRG 1 cut(s) 775
BslFI GGGAC 3 cut(s) 63, 223, 785
BsmAI GTCTC 1 cut(s) 538
BsmBI CGTCTC 1 cut(s) 538
BsmFI GGGAC 3 cut(s) 63, 223, 785
BsoBI CYCGRG 1 cut(s) 775
Bsp120I GGGCCC 1 cut(s) 887
Bsp1286I GDGCHC 3 cut(s) 354, 833, 891
Bsp13I TCCGGA 1 cut(s) 792
Bsp143I GATC 8 cut(s) 178, 306, 469, 486, 501, 648, 696, 742
BspCNI CTCAG 3 cut(s) 294, 738, 891
BspEI TCCGGA 1 cut(s) 792
BspFNI CGCG 5 cut(s) 393, 567, 591, 701, 800
BspPI GGATC 2 cut(s) 494, 750
BspT107I GGYRCC 2 cut(s) 762, 830
BsrBI CCGCTC 1 cut(s) 689
BsrFI RCCGGY 2 cut(s) 311, 557
BsrI ACTGG 2 cut(s) 75, 1058
BssAI RCCGGY 2 cut(s) 311, 557
BssECI CCNNGG 9 cut(s) 358, 372, 414, 450, 513, 565, 775, 776, 891
BssMI GATC 8 cut(s) 178, 306, 469, 486, 501, 648, 696, 742
BssNI GRCGYC 2 cut(s) 561, 939
BssT1I CCWWGG 1 cut(s) 513
Bst2UI CCWGG 3 cut(s) 451, 659, 893
Bst4CI ACNGT 2 cut(s) 196, 583
Bst6I CTCTTC 3 cut(s) 76, 278, 677
BstACI GRCGYC 2 cut(s) 561, 939
BstBAI YACGTR 1 cut(s) 908
BstC8I GCNNGC 6 cut(s) 313, 479, 559, 725, 754, 991
BstDEI CTNAG 3 cut(s) 281, 746, 899
BstDSI CCRYGG 1 cut(s) 565
BstF5I GGATG 1 cut(s) 125
BstFNI CGCG 5 cut(s) 393, 567, 591, 701, 800
BstH2I RGCGCY 1 cut(s) 753
BstHHI GCGC 4 cut(s) 395, 593, 752, 959
BstKTI GATC 8 cut(s) 181, 309, 472, 489, 504, 651, 699, 745
BstMAI GTCTC 1 cut(s) 538
BstMBI GATC 8 cut(s) 178, 306, 469, 486, 501, 648, 696, 742
BstMCI CGRYCG 1 cut(s) 705
BstNI CCWGG 3 cut(s) 451, 659, 893
BstSCI CCNGG 7 cut(s) 358, 414, 449, 657, 774, 775, 891
BstSLI GKGCMC 2 cut(s) 833, 891
BstUI CGCG 5 cut(s) 393, 567, 591, 701, 800
BstV1I GCAGC 3 cut(s) 417, 796, 971
BstX2I RGATCY 2 cut(s) 469, 742
BstYI RGATCY 2 cut(s) 469, 742
BstZI CGGCCG 1 cut(s) 702
Bsu36I CCTNAGG 1 cut(s) 899
BtgI CCRYGG 1 cut(s) 565
BtrI CACGTC 1 cut(s) 973
BtsCI GGATG 1 cut(s) 125
BtsIMutI CAGTG 1 cut(s) 8
Cac8I GCNNGC 6 cut(s) 313, 479, 559, 725, 754, 991
CaiI CAGNNNCTG 1 cut(s) 898
CfoI GCGC 4 cut(s) 395, 593, 752, 959
Cfr10I RCCGGY 2 cut(s) 311, 557
Cfr42I CCGCGG 1 cut(s) 568
Cfr9I CCCGGG 1 cut(s) 775
CseI GACGC 1 cut(s) 550
Csp6I GTAC 2 cut(s) 763, 1051
CviAII CATG 5 cut(s) 346, 490, 652, 919, 1028
CviQI GTAC 2 cut(s) 763, 1051
DdeI CTNAG 3 cut(s) 281, 746, 899
DpnI GATC 8 cut(s) 180, 308, 471, 488, 503, 650, 698, 744
DpnII GATC 8 cut(s) 178, 306, 469, 486, 501, 648, 696, 742
DraIII CACNNNGTG 1 cut(s) 922
DrdI GACNNNNNNGTC 1 cut(s) 335
DseDI GACNNNNNNGTC 1 cut(s) 335
EaeI YGGCCR 2 cut(s) 3, 702
EagI CGGCCG 1 cut(s) 702
Eam1104I CTCTTC 3 cut(s) 76, 278, 677
EarI CTCTTC 3 cut(s) 76, 278, 677
EciI GGCGGA 1 cut(s) 562
EclXI CGGCCG 1 cut(s) 702
Eco130I CCWWGG 1 cut(s) 513
Eco24I GRGCYC 1 cut(s) 891
Eco47I GGWCC 4 cut(s) 637, 772, 790, 895
Eco52I CGGCCG 1 cut(s) 702
Eco72I CACGTG 1 cut(s) 908
Eco81I CCTNAGG 1 cut(s) 899
Eco88I CYCGRG 1 cut(s) 775
EcoO109I RGGNCCY 5 cut(s) 376, 772, 852, 888, 895
EcoRII CCWGG 3 cut(s) 449, 657, 891
EcoT14I CCWWGG 1 cut(s) 513
EcoT38I GRGCYC 1 cut(s) 891
ErhI CCWWGG 1 cut(s) 513
Esp3I CGTCTC 1 cut(s) 538
FaeI CATG 5 cut(s) 349, 493, 655, 922, 1031
FaiI YATR 6 cut(s) 347, 491, 653, 875, 920, 1029
FaqI GGGAC 3 cut(s) 63, 223, 785
FatI CATG 5 cut(s) 345, 489, 651, 918, 1027
FauI CCCGC 4 cut(s) 231, 248, 486, 562
FbaI TGATCA 1 cut(s) 648
FblI GTMKAC 2 cut(s) 342, 936
FokI GGATG 1 cut(s) 112
FriOI GRGCYC 1 cut(s) 891
FspBI CTAG 1 cut(s) 467
GlaI GCGC 4 cut(s) 394, 592, 751, 958
GsaI CCCAGC 2 cut(s) 830, 867
GsuI CTGGAG 1 cut(s) 580
HaeII RGCGCY 1 cut(s) 753
HgaI GACGC 1 cut(s) 550
HhaI GCGC 4 cut(s) 395, 593, 752, 959
Hin1I GRCGYC 2 cut(s) 561, 939
Hin1II CATG 5 cut(s) 349, 493, 655, 922, 1031
Hin6I GCGC 4 cut(s) 393, 591, 750, 957
HinP1I GCGC 4 cut(s) 393, 591, 750, 957
HincII GTYRAC 3 cut(s) 343, 718, 937
HindII GTYRAC 3 cut(s) 343, 718, 937
HinfI GANTC 3 cut(s) 13, 421, 437
HphI GGTGA 6 cut(s) 31, 42, 136, 184, 190, 1043
Hpy166II GTNNAC 3 cut(s) 343, 718, 937
Hpy188I TCNGA 4 cut(s) 178, 373, 747, 1009
Hpy188III TCNNGA 2 cut(s) 496, 793
Hpy8I GTNNAC 3 cut(s) 343, 718, 937
Hpy99I CGWCG 9 cut(s) 338, 341, 344, 587, 614, 710, 938, 941, 944
HpyAV CCTTC 4 cut(s) 704, 781, 799, 811
HpyCH4III ACNGT 2 cut(s) 196, 583
HpyCH4IV ACGT 3 cut(s) 907, 939, 972
HpyCH4V TGCA 2 cut(s) 727, 1040
HpyF3I CTNAG 3 cut(s) 281, 746, 899
HpySE526I ACGT 3 cut(s) 907, 939, 972
Hsp92I GRCGYC 2 cut(s) 561, 939
Hsp92II CATG 5 cut(s) 349, 493, 655, 922, 1031
HspAI GCGC 4 cut(s) 393, 591, 750, 957
KflI GGGWCCC 1 cut(s) 772
Kpn2I TCCGGA 1 cut(s) 792
KpnI GGTACC 1 cut(s) 766
KroI GCCGGC 2 cut(s) 311, 557
KroNI GCCGGC 2 cut(s) 313, 559
Ksp22I TGATCA 1 cut(s) 648
KspI CCGCGG 1 cut(s) 568
Kzo9I GATC 8 cut(s) 178, 306, 469, 486, 501, 648, 696, 742
LmnI GCTCC 6 cut(s) 349, 515, 575, 954, 1024, 1056
Lsp1109I GCAGC 3 cut(s) 417, 796, 971
LweI GCATC 3 cut(s) 375, 615, 1027
MaeI CTAG 1 cut(s) 467
MaeII ACGT 3 cut(s) 907, 939, 972
MaeIII GTNAC 1 cut(s) 196
MalI GATC 8 cut(s) 180, 308, 471, 488, 503, 650, 698, 744
MbiI CCGCTC 1 cut(s) 689
MboI GATC 8 cut(s) 178, 306, 469, 486, 501, 648, 696, 742
MboII GAAGA 3 cut(s) 63, 265, 694
MfeI CAATTG 1 cut(s) 641
MflI RGATCY 2 cut(s) 469, 742
MhlI GDGCHC 3 cut(s) 354, 833, 891
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 628, 641
MluI ACGCGT 1 cut(s) 798
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 720
Mox20I TGGCCA 1 cut(s) 5
MreI CGCCGGCG 1 cut(s) 557
MroI TCCGGA 1 cut(s) 792
MroNI GCCGGC 2 cut(s) 311, 557
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 999
MslI CAYNNNNRTG 1 cut(s) 1032
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 567, 962
MspR9I CCNGG 7 cut(s) 360, 416, 451, 659, 776, 777, 893
MunI CAATTG 1 cut(s) 641
MvaI CCWGG 3 cut(s) 451, 659, 893
MvnI CGCG 5 cut(s) 393, 567, 591, 701, 800
NaeI GCCGGC 2 cut(s) 313, 559
NciI CCSGG 4 cut(s) 360, 416, 776, 777
NdeII GATC 8 cut(s) 178, 306, 469, 486, 501, 648, 696, 742
NgoMIV GCCGGC 2 cut(s) 311, 557
NlaIII CATG 5 cut(s) 349, 493, 655, 922, 1031
NmeAIII GCCGAG 4 cut(s) 328, 453, 547, 838
NmuCI GTSAC 1 cut(s) 196
PcsI WCGNNNNNNNCGW 4 cut(s) 333, 339, 609, 705
PdiI GCCGGC 2 cut(s) 313, 559
PfeI GAWTC 2 cut(s) 421, 437
PflFI GACNNNGTC 1 cut(s) 332
PleI GAGTC 1 cut(s) 7
PmaCI CACGTG 1 cut(s) 908
PmlI CACGTG 1 cut(s) 908
PpsI GAGTC 1 cut(s) 7
Ppu21I YACGTR 1 cut(s) 908
PpuMI RGGWCCY 2 cut(s) 772, 895
Psp5II RGGWCCY 2 cut(s) 772, 895
Psp6I CCWGG 3 cut(s) 449, 657, 891
PspCI CACGTG 1 cut(s) 908
PspFI CCCAGC 2 cut(s) 826, 863
PspGI CCWGG 3 cut(s) 449, 657, 891
PspOMI GGGCCC 1 cut(s) 887
PspPPI RGGWCCY 2 cut(s) 772, 895
PstNI CAGNNNCTG 1 cut(s) 898
PsuI RGATCY 2 cut(s) 469, 742
PsyI GACNNNGTC 1 cut(s) 332
RsaI GTAC 2 cut(s) 764, 1052
RsaNI GTAC 2 cut(s) 763, 1051
RseI CAYNNNNRTG 1 cut(s) 1032
SacII CCGCGG 1 cut(s) 568
SalI GTCGAC 2 cut(s) 341, 935
SaqAI TTAA 1 cut(s) 999
Sau3AI GATC 8 cut(s) 178, 306, 469, 486, 501, 648, 696, 742
ScaI AGTACT 1 cut(s) 1052
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 7 cut(s) 360, 416, 451, 659, 776, 777, 893
SduI GDGCHC 3 cut(s) 354, 833, 891
SfaNI GCATC 3 cut(s) 375, 615, 1027
Sfr303I CCGCGG 1 cut(s) 568
SgrAI CRCCGGYG 1 cut(s) 557
SgrBI CCGCGG 1 cut(s) 568
SgrDI CGTCGACG 1 cut(s) 935
SinI GGWCC 4 cut(s) 637, 772, 790, 895
SmaI CCCGGG 1 cut(s) 777
SmiMI CAYNNNNRTG 1 cut(s) 1032
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
Sse9I AATT 2 cut(s) 628, 641
SspMI CTAG 1 cut(s) 467
StyD4I CCNGG 7 cut(s) 358, 414, 449, 657, 774, 775, 891
StyI CCWWGG 1 cut(s) 513
TaaI ACNGT 2 cut(s) 196, 583
TaiI ACGT 3 cut(s) 910, 942, 975
TaqI TCGA 5 cut(s) 342, 504, 609, 708, 936
TasI AATT 2 cut(s) 628, 641
TatI WGTACW 1 cut(s) 1050
TfiI GAWTC 2 cut(s) 421, 437
Tru1I TTAA 1 cut(s) 999
Tru9I TTAA 1 cut(s) 999
TscAI CASTG 1 cut(s) 15
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 3 cut(s) 430, 809, 959
Tsp45I GTSAC 1 cut(s) 196
TspGWI ACGGA 1 cut(s) 922
TspMI CCCGGG 1 cut(s) 775
TspRI CASTG 1 cut(s) 15
Tth111I GACNNNGTC 1 cut(s) 332
VpaK11BI GGWCC 4 cut(s) 637, 772, 790, 895
XmaI CCCGGG 1 cut(s) 775
XmiI GTMKAC 2 cut(s) 342, 936
XspI CTAG 1 cut(s) 467
ZraI GACGTC 1 cut(s) 940
ZrmI AGTACT 1 cut(s) 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.