AT4G12590

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0008511
Physical Location & Seq
Reverse (-)
7450943 .. 7453142
2200 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G12590.1

Sequence Viewer

Length: 741 bp
ATGGCGGAGGATCTAGTTCTCGATACGGCGATCAGAGATTGGGTTCTGATTCCTCTCTCCGTAGTGATGGTTCTAATCGGTATTTTGAGATACTTTGTCTCTAAGCTTATGCGTTCTACTCCAACTCCTGATGCTAAGATGGTTAAAGAAGGGCAAGTTGTTATTAGAGCTCGGAATCTTAAAGTCGGTGCTAATTTTATCCCGCCGAAATCATTCCGTGCTCGGAGATTCTACTTCAGCAACGAGGAGAATGGTTTGCTACATGTTCCCAAGGGAGAAGCTCAAAATCCACAAGCTGCTATGTTTTCTGATCCTAACATGGCCATGGATATGATGAAGAAAAATCTTTCTATGATTATTCCTCAGACACTCACTTTTGCATGGGTCAACTTCTTCTTCTCTGGATTTGTTGCAGCCAAAATACCATTTCCACTGACTCAGAGGTTCAGATCAATGTTACAGAATGGCATTGACTTGAGCACTGTCGATGTTAGCTATGTGAGTAGTCGTTCCTGGTACTTCTTGAACTTGTTTGGATTAAGAGGCTTGTTCAGTCTCATTCTCGGAGATGAAAATGCCATTGATGACACACAACGTATGATGCAAATGGGTGGGTTTGGATTTGATGCATCAAAGAGTCTGGGTGCAGAGAAGGATGGTTTAGACATAATCCAGCACGAATGGGCACTACCTCGATTTGAGCAGCGCGCAGAATCTGTATTAAGAAAACTCGTGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF106

Protein Analysis

246

Amino Acids

27.91

Weight (kDa)

9.48

Isoelectric Point (pI)

25.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC3_TMCO1 PF01956 4 - 192 3.6e-52 Integral membrane protein EMC3/TMCO1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 708
AciI CCGC 2 cut(s) 5, 203
AclWI GGATC 2 cut(s) 18, 305
AcoI YGGCCR 1 cut(s) 321
AcuI CTGAAG 1 cut(s) 220
AfaI GTAC 1 cut(s) 518
AflIII ACRYGT 1 cut(s) 262
AgsI TTSAA 1 cut(s) 526
AjnI CCWGG 1 cut(s) 512
AluBI AGCT 5 cut(s) 106, 170, 281, 296, 495
AluI AGCT 5 cut(s) 106, 170, 281, 296, 495
Alw21I GWGCWC 3 cut(s) 172, 223, 482
Alw26I GTCTC 2 cut(s) 103, 560
AlwI GGATC 2 cut(s) 18, 305
AlwNI CAGNNNCTG 1 cut(s) 716
AoxI GGCC 1 cut(s) 321
ApeKI GCWGC 3 cut(s) 296, 413, 703
Asp700I GAANNNNTTC 1 cut(s) 212
AspLEI GCGC 2 cut(s) 708, 710
BaeGI GKGCMC 1 cut(s) 688
BalI TGGCCA 1 cut(s) 323
BanII GRGCYC 1 cut(s) 172
BauI CACGAG 1 cut(s) 731
Bbv12I GWGCWC 3 cut(s) 172, 223, 482
BbvI GCAGC 3 cut(s) 283, 425, 715
BccI CCATC 3 cut(s) 61, 133, 650
BceAI ACGGC 1 cut(s) 42
BciT130I CCWGG 1 cut(s) 514
BcoDI GTCTC 2 cut(s) 103, 560
BfaI CTAG 1 cut(s) 14
BisI GCNGC 3 cut(s) 297, 414, 704
BlsI GCNGC 3 cut(s) 298, 415, 705
Bme1390I CCNGG 1 cut(s) 514
BmrFI CCNGG 1 cut(s) 514
BmsI GCATC 4 cut(s) 121, 591, 616, 638
BpuEI CTTGAG 1 cut(s) 496
BsaJI CCNNGG 2 cut(s) 270, 324
BsaXI ACNNNNNCTCC 2 cut(s) 109, 139
BseBI CCWGG 1 cut(s) 514
BseDI CCNNGG 2 cut(s) 270, 324
BseGI GGATG 1 cut(s) 661
BseMII CTCAG 2 cut(s) 377, 452
BsePI GCGCGC 1 cut(s) 706
BseRI GAGGAG 1 cut(s) 260
BseSI GKGCMC 1 cut(s) 688
BseXI GCAGC 3 cut(s) 283, 425, 715
BsgI GTGCAG 1 cut(s) 666
Bsh1236I CGCG 1 cut(s) 708
BshFI GGCC 1 cut(s) 323
BsiHKAI GWGCWC 3 cut(s) 172, 223, 482
BsmAI GTCTC 2 cut(s) 103, 560
BsnI GGCC 1 cut(s) 323
Bsp1286I GDGCHC 4 cut(s) 172, 223, 482, 688
Bsp143I GATC 4 cut(s) 10, 30, 310, 449
Bsp19I CCATGG 1 cut(s) 324
BspACI CCGC 2 cut(s) 5, 203
BspANI GGCC 1 cut(s) 323
BspCNI CTCAG 2 cut(s) 376, 451
BspFNI CGCG 1 cut(s) 708
BspPI GGATC 2 cut(s) 18, 305
BssECI CCNNGG 2 cut(s) 270, 324
BssHII GCGCGC 1 cut(s) 706
BssMI GATC 4 cut(s) 10, 30, 310, 449
BssSI CACGAG 1 cut(s) 731
BssT1I CCWWGG 2 cut(s) 270, 324
Bst2BI CACGAG 1 cut(s) 731
Bst2UI CCWGG 1 cut(s) 514
Bst4CI ACNGT 1 cut(s) 484
BstC8I GCNNGC 1 cut(s) 708
BstDEI CTNAG 4 cut(s) 102, 135, 363, 438
BstDSI CCRYGG 1 cut(s) 324
BstF5I GGATG 1 cut(s) 661
BstFNI CGCG 1 cut(s) 708
BstHHI GCGC 2 cut(s) 708, 710
BstKTI GATC 4 cut(s) 13, 33, 313, 452
BstMAI GTCTC 2 cut(s) 103, 560
BstMBI GATC 4 cut(s) 10, 30, 310, 449
BstNI CCWGG 1 cut(s) 514
BstNSI RCATGY 1 cut(s) 266
BstSCI CCNGG 1 cut(s) 512
BstSLI GKGCMC 1 cut(s) 688
BstUI CGCG 1 cut(s) 708
BstV1I GCAGC 3 cut(s) 283, 425, 715
BstX2I RGATCY 1 cut(s) 10
BstYI RGATCY 1 cut(s) 10
BsuRI GGCC 1 cut(s) 323
BtgI CCRYGG 1 cut(s) 324
BtsCI GGATG 1 cut(s) 661
BtsIMutI CAGTG 2 cut(s) 431, 480
Cac8I GCNNGC 1 cut(s) 708
CaiI CAGNNNCTG 1 cut(s) 716
CfoI GCGC 2 cut(s) 708, 710
Csp6I GTAC 1 cut(s) 517
CviAII CATG 4 cut(s) 263, 319, 325, 381
CviJI RGCY 8 cut(s) 106, 170, 281, 296, 323, 416, 495, 546
CviKI_1 RGCY 8 cut(s) 106, 170, 281, 296, 323, 416, 495, 546
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 4 cut(s) 102, 135, 363, 438
DpnI GATC 4 cut(s) 12, 32, 312, 451
DpnII GATC 4 cut(s) 10, 30, 310, 449
EaeI YGGCCR 1 cut(s) 321
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 170
Eco130I CCWWGG 2 cut(s) 270, 324
Eco24I GRGCYC 1 cut(s) 172
Eco53kI GAGCTC 1 cut(s) 170
Eco57I CTGAAG 1 cut(s) 220
EcoICRI GAGCTC 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 512
EcoT14I CCWWGG 2 cut(s) 270, 324
EcoT22I ATGCAT 1 cut(s) 631
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 2 cut(s) 270, 324
FaeI CATG 4 cut(s) 266, 322, 328, 384
FatI CATG 4 cut(s) 262, 318, 324, 380
FauI CCCGC 1 cut(s) 210
Fnu4HI GCNGC 3 cut(s) 297, 414, 704
FokI GGATG 1 cut(s) 668
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 3 cut(s) 297, 414, 704
FspBI CTAG 1 cut(s) 14
GlaI GCGC 2 cut(s) 707, 709
GluI GCNGC 3 cut(s) 297, 414, 704
HaeIII GGCC 1 cut(s) 323
HhaI GCGC 2 cut(s) 708, 710
Hin1II CATG 4 cut(s) 266, 322, 328, 384
Hin6I GCGC 2 cut(s) 706, 708
HinP1I GCGC 2 cut(s) 706, 708
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HindIII AAGCTT 1 cut(s) 104
HinfI GANTC 6 cut(s) 49, 175, 228, 436, 637, 713
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 9 cut(s) 35, 48, 174, 225, 310, 366, 441, 449, 566
Hpy188III TCNNGA 5 cut(s) 20, 128, 402, 523, 733
Hpy8I GTNNAC 1 cut(s) 388
HpyAV CCTTC 2 cut(s) 143, 646
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4IV ACGT 1 cut(s) 595
HpyCH4V TGCA 5 cut(s) 380, 413, 604, 629, 647
HpyF3I CTNAG 4 cut(s) 102, 135, 363, 438
HpySE526I ACGT 1 cut(s) 595
Hsp92II CATG 4 cut(s) 266, 322, 328, 384
HspAI GCGC 2 cut(s) 706, 708
Kzo9I GATC 4 cut(s) 10, 30, 310, 449
LpnPI CCDG 6 cut(s) 141, 387, 499, 526, 626, 686
Lsp1109I GCAGC 3 cut(s) 283, 425, 715
LweI GCATC 4 cut(s) 121, 591, 616, 638
MaeI CTAG 1 cut(s) 14
MaeII ACGT 1 cut(s) 595
MaeIII GTNAC 1 cut(s) 456
MalI GATC 4 cut(s) 12, 32, 312, 451
MboI GATC 4 cut(s) 10, 30, 310, 449
MboII GAAGA 3 cut(s) 349, 385, 388
MflI RGATCY 1 cut(s) 10
MhlI GDGCHC 4 cut(s) 172, 223, 482, 688
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 1 cut(s) 193
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 2 cut(s) 430, 646
MmeI TCCRAC 1 cut(s) 146
MnlI CCTC 6 cut(s) 63, 238, 372, 435, 536, 702
Mox20I TGGCCA 1 cut(s) 323
Mph1103I ATGCAT 1 cut(s) 631
MroXI GAANNNNTTC 1 cut(s) 212
MscI TGGCCA 1 cut(s) 323
MseI TTAA 4 cut(s) 144, 180, 539, 722
MslI CAYNNNNRTG 2 cut(s) 323, 329
Msp20I TGGCCA 1 cut(s) 323
MspR9I CCNGG 1 cut(s) 514
MvaI CCWGG 1 cut(s) 514
MvnI CGCG 1 cut(s) 708
NcoI CCATGG 1 cut(s) 324
NdeII GATC 4 cut(s) 10, 30, 310, 449
NlaIII CATG 4 cut(s) 266, 322, 328, 384
NsiI ATGCAT 1 cut(s) 631
NspI RCATGY 1 cut(s) 266
PauI GCGCGC 1 cut(s) 706
PciI ACATGT 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 4 cut(s) 49, 175, 228, 713
PkrI GCNGC 3 cut(s) 298, 415, 705
PleI GAGTC 2 cut(s) 430, 645
PpsI GAGTC 2 cut(s) 430, 645
PscI ACATGT 1 cut(s) 262
Psp124BI GAGCTC 1 cut(s) 172
Psp6I CCWGG 1 cut(s) 512
PspGI CCWGG 1 cut(s) 512
PsrI GAACNNNNNNTAC 2 cut(s) 54, 86
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 1 cut(s) 10
PteI GCGCGC 1 cut(s) 706
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
RseI CAYNNNNRTG 2 cut(s) 323, 329
SacI GAGCTC 1 cut(s) 172
SaqAI TTAA 4 cut(s) 144, 180, 539, 722
SatI GCNGC 3 cut(s) 297, 414, 704
Sau3AI GATC 4 cut(s) 10, 30, 310, 449
SchI GAGTC 2 cut(s) 430, 646
ScrFI CCNGG 1 cut(s) 514
SduI GDGCHC 4 cut(s) 172, 223, 482, 688
SetI ASST 8 cut(s) 108, 172, 283, 298, 446, 497, 598, 694
SfaNI GCATC 4 cut(s) 121, 591, 616, 638
SmiMI CAYNNNNRTG 2 cut(s) 323, 329
SmlI CTYRAG 1 cut(s) 475
SmoI CTYRAG 1 cut(s) 475
Sse9I AATT 1 cut(s) 193
SsiI CCGC 2 cut(s) 5, 203
SspMI CTAG 1 cut(s) 14
SstI GAGCTC 1 cut(s) 172
StyD4I CCNGG 1 cut(s) 512
StyI CCWWGG 2 cut(s) 270, 324
TaaI ACNGT 1 cut(s) 484
TaiI ACGT 1 cut(s) 598
TaqI TCGA 3 cut(s) 21, 486, 694
TasI AATT 1 cut(s) 193
TfiI GAWTC 4 cut(s) 49, 175, 228, 713
Tru1I TTAA 4 cut(s) 144, 180, 539, 722
Tru9I TTAA 4 cut(s) 144, 180, 539, 722
TscAI CASTG 2 cut(s) 438, 487
TseI GCWGC 3 cut(s) 296, 413, 703
TspDTI ATGAA 2 cut(s) 350, 585
TspGWI ACGGA 2 cut(s) 49, 206
TspRI CASTG 2 cut(s) 438, 487
XceI RCATGY 1 cut(s) 266
XmnI GAANNNNTTC 1 cut(s) 212
XspI CTAG 1 cut(s) 14
Zsp2I ATGCAT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.