MD10G1332200.v1.1

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0008511
Physical Location & Seq
Reverse (-)
40968399 .. 40973018
4620 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1332200.v1.1.491

Sequence Viewer

Length: 324 bp
ATGCGATCCAACCAAGTTCCCGATGCCAAAATTGTCAAAGAAGGGCAATTGATTATTAGGGCTCGGAACTTACGTGCTGCTGCTAATTTCATACCTCCAAAGTCGTTTCGTGCTCGCCGATTGTATTACAACAACGAGGAAAATGGGTTGTTAATTGTTCCTAAGGGTCAGGCACAGAATCCACAAGCACAAATGTTCTCTGATCCCAACATGGCCATGGATATGATGAAGAAAAATCTGTCCATGATAATACCCCAGACTCTTACTTTTGCATGGGTCAACTTTTTCTTCTCTGGATTTGTAGCAGGGTTTACTAGCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF106

Protein Analysis

108

Amino Acids

12.17

Weight (kDa)

10.43

Isoelectric Point (pI)

25.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC3_TMCO1 PF01956 11 - 102 7.6e-15 Integral membrane protein EMC3/TMCO1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 197
AcoI YGGCCR 1 cut(s) 213
Alw21I GWGCWC 1 cut(s) 115
AlwI GGATC 1 cut(s) 197
AoxI GGCC 1 cut(s) 213
ApeKI GCWGC 2 cut(s) 77, 80
AxyI CCTNAGG 1 cut(s) 162
BalI TGGCCA 1 cut(s) 215
BanII GRGCYC 1 cut(s) 64
Bbv12I GWGCWC 1 cut(s) 115
BbvI GCAGC 2 cut(s) 64, 67
BfaI CTAG 1 cut(s) 315
BisI GCNGC 2 cut(s) 78, 81
BlsI GCNGC 2 cut(s) 79, 82
BmsI GCATC 1 cut(s) 13
BsaAI YACGTR 1 cut(s) 74
BsaJI CCNNGG 1 cut(s) 216
Bse21I CCTNAGG 1 cut(s) 162
BseDI CCNNGG 1 cut(s) 216
BseXI GCAGC 2 cut(s) 64, 67
BshFI GGCC 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 115
BsnI GGCC 1 cut(s) 215
Bsp1286I GDGCHC 2 cut(s) 64, 115
Bsp143I GATC 2 cut(s) 5, 202
Bsp19I CCATGG 1 cut(s) 216
BspANI GGCC 1 cut(s) 215
BspPI GGATC 1 cut(s) 197
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 2 cut(s) 5, 202
BssT1I CCWWGG 1 cut(s) 216
BstBAI YACGTR 1 cut(s) 74
BstC8I GCNNGC 1 cut(s) 115
BstDEI CTNAG 1 cut(s) 162
BstDSI CCRYGG 1 cut(s) 216
BstKTI GATC 2 cut(s) 8, 205
BstMBI GATC 2 cut(s) 5, 202
BstV1I GCAGC 2 cut(s) 64, 67
Bsu36I CCTNAGG 1 cut(s) 162
BsuRI GGCC 1 cut(s) 215
BtgI CCRYGG 1 cut(s) 216
Cac8I GCNNGC 1 cut(s) 115
CviAII CATG 4 cut(s) 211, 217, 244, 273
CviJI RGCY 3 cut(s) 62, 215, 318
CviKI_1 RGCY 3 cut(s) 62, 215, 318
DdeI CTNAG 1 cut(s) 162
DpnI GATC 2 cut(s) 7, 204
DpnII GATC 2 cut(s) 5, 202
EaeI YGGCCR 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 216
Eco24I GRGCYC 1 cut(s) 64
Eco81I CCTNAGG 1 cut(s) 162
EcoT14I CCWWGG 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 4 cut(s) 214, 220, 247, 276
FaiI YATR 7 cut(s) 92, 212, 218, 224, 245, 274, 322
FatI CATG 4 cut(s) 210, 216, 243, 272
Fnu4HI GCNGC 2 cut(s) 78, 81
FriOI GRGCYC 1 cut(s) 64
Fsp4HI GCNGC 2 cut(s) 78, 81
FspBI CTAG 1 cut(s) 315
GluI GCNGC 2 cut(s) 78, 81
HaeIII GGCC 1 cut(s) 215
Hin1II CATG 4 cut(s) 214, 220, 247, 276
HincII GTYRAC 1 cut(s) 280
HindII GTYRAC 1 cut(s) 280
HinfI GANTC 2 cut(s) 178, 259
Hpy166II GTNNAC 2 cut(s) 280, 312
Hpy188I TCNGA 2 cut(s) 66, 202
Hpy188III TCNNGA 2 cut(s) 20, 294
Hpy8I GTNNAC 2 cut(s) 280, 312
HpyAV CCTTC 1 cut(s) 35
HpyCH4IV ACGT 1 cut(s) 73
HpyCH4V TGCA 1 cut(s) 272
HpyF3I CTNAG 1 cut(s) 162
HpySE526I ACGT 1 cut(s) 73
Hsp92II CATG 4 cut(s) 214, 220, 247, 276
Kzo9I GATC 2 cut(s) 5, 202
LpnPI CCDG 4 cut(s) 155, 269, 279, 291
Lsp1109I GCAGC 2 cut(s) 64, 67
LweI GCATC 1 cut(s) 13
MaeI CTAG 1 cut(s) 315
MaeII ACGT 1 cut(s) 73
MalI GATC 2 cut(s) 7, 204
MboI GATC 2 cut(s) 5, 202
MboII GAAGA 2 cut(s) 241, 280
MfeI CAATTG 1 cut(s) 47
MhlI GDGCHC 2 cut(s) 64, 115
MlsI TGGCCA 1 cut(s) 215
MluCI AATT 4 cut(s) 30, 47, 85, 153
MluNI TGGCCA 1 cut(s) 215
MlyI GAGTC 1 cut(s) 253
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 2 cut(s) 105, 130
Mox20I TGGCCA 1 cut(s) 215
MscI TGGCCA 1 cut(s) 215
MseI TTAA 1 cut(s) 152
MslI CAYNNNNRTG 2 cut(s) 215, 221
Msp20I TGGCCA 1 cut(s) 215
MunI CAATTG 1 cut(s) 47
NcoI CCATGG 1 cut(s) 216
NdeII GATC 2 cut(s) 5, 202
NlaIII CATG 4 cut(s) 214, 220, 247, 276
PcsI WCGNNNNNNNCGW 2 cut(s) 70, 115
PfeI GAWTC 1 cut(s) 178
PkrI GCNGC 2 cut(s) 79, 82
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
Ppu21I YACGTR 1 cut(s) 74
RseI CAYNNNNRTG 2 cut(s) 215, 221
SaqAI TTAA 1 cut(s) 152
SatI GCNGC 2 cut(s) 78, 81
Sau3AI GATC 2 cut(s) 5, 202
SchI GAGTC 1 cut(s) 253
SduI GDGCHC 2 cut(s) 64, 115
SetI ASST 2 cut(s) 76, 97
SfaNI GCATC 1 cut(s) 13
SmiMI CAYNNNNRTG 2 cut(s) 215, 221
Sse9I AATT 4 cut(s) 30, 47, 85, 153
SspMI CTAG 1 cut(s) 315
StyI CCWWGG 1 cut(s) 216
TaiI ACGT 1 cut(s) 76
TasI AATT 4 cut(s) 30, 47, 85, 153
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 1 cut(s) 152
Tru9I TTAA 1 cut(s) 152
TseI GCWGC 2 cut(s) 77, 80
TspDTI ATGAA 2 cut(s) 79, 242
XcmI CCANNNNNNNNNTGG 1 cut(s) 214
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.