MD05G1358200.v1.1

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008511
Physical Location & Seq
Forward (+)
47355586 .. 47358831
3246 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1358200.v1.1.491

Sequence Viewer

Length: 741 bp
ATGGCGGCAGATCTGGTGCTCGACACAGCCATCAGAGACTGGGTTCTGATCCCTCTCTCGGTGGTCATGGTTCTCATCGGCGTCCTCCGATACTTCGTCTCCAAGCTCATGCGCTCCAACCAAGTCCCCGATGCCAAAATCGTCAAGGAAGGGCAATTGATTATTAGGGCTCGGAATTTACGTGCTGCTGCTAATTTCATACCTCCAAAGTCGTTTCGTGCTCGCCGATTGTATTACAACAACGAGGAAAATGGGCTGTTAATTGTTCCTAAGGGTCAGGCACAGAATCCACAAGCGCAAATGTTCTCTGATCCCAACATGGCCATGGATATGATGAAGAAAAATCTGTCCATGATAATACCCCAGACTCTTACTTTTGCATGGGTCAATTTTTTCTTCTCTGGATTTGTTGCAGCCAAAATTCCCTTTCCTCTTACTCAAAGGTTCAGGTCTATGCTGCAGAATGGGATTGACTTGAGCACTGTTGATGTTAGCTACGTCAGTAGCCGCTCATGGTACTTTCTTAATCTGTTTGGATTAAGAGGTTTGTTCAGTCTCATTCTGGGAGAAGAAAATGCCATGGATGATACTCAAAAAATGATGCAAATGAGTGGCTTTGGCTTTGATCCATCAAAGAGTTTGGGAGCTGAGAAGGACAGCCTCGATATAGTCCAGCATGACTGGGCCTTACCAAAATTTGAGCATCGCGCTGAAGCCGTATTGAGAAAACTTGCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF106

Protein Analysis

247

Amino Acids

27.98

Weight (kDa)

9.69

Isoelectric Point (pI)

27.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC3_TMCO1 PF01956 4 - 192 1.5e-52 Integral membrane protein EMC3/TMCO1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 510
AccII CGCG 1 cut(s) 708
AciI CCGC 2 cut(s) 5, 508
AclWI GGATC 3 cut(s) 43, 305, 620
AcoI YGGCCR 1 cut(s) 321
AcsI RAATTY 3 cut(s) 175, 420, 695
AcuI CTGAAG 1 cut(s) 732
AcyI GRCGYC 1 cut(s) 81
AfaI GTAC 1 cut(s) 518
AfiI CCNNNNNNNGG 1 cut(s) 58
AluBI AGCT 3 cut(s) 106, 495, 647
AluI AGCT 3 cut(s) 106, 495, 647
Alw21I GWGCWC 3 cut(s) 21, 223, 482
Alw26I GTCTC 3 cut(s) 30, 103, 560
AlwI GGATC 3 cut(s) 43, 305, 620
AlwNI CAGNNNCTG 1 cut(s) 39
AoxI GGCC 2 cut(s) 321, 684
ApeKI GCWGC 4 cut(s) 185, 188, 413, 457
ApoI RAATTY 3 cut(s) 175, 420, 695
AspLEI GCGC 3 cut(s) 114, 298, 710
AspS9I GGNCC 1 cut(s) 684
AxyI CCTNAGG 1 cut(s) 270
BalI TGGCCA 1 cut(s) 323
BanII GRGCYC 1 cut(s) 172
Bbv12I GWGCWC 3 cut(s) 21, 223, 482
BbvI GCAGC 4 cut(s) 172, 175, 425, 444
BccI CCATC 2 cut(s) 38, 637
BceAI ACGGC 1 cut(s) 701
BcoDI GTCTC 3 cut(s) 30, 103, 560
BfmI CTRYAG 1 cut(s) 458
BglII AGATCT 1 cut(s) 10
BisI GCNGC 6 cut(s) 6, 186, 189, 414, 458, 508
BlsI GCNGC 6 cut(s) 7, 187, 190, 415, 459, 509
BmgT120I GGNCC 1 cut(s) 684
BmrI ACTGGG 2 cut(s) 49, 691
BmsI GCATC 3 cut(s) 121, 591, 712
BmuI ACTGGG 2 cut(s) 49, 691
BpuEI CTTGAG 1 cut(s) 496
BsaAI YACGTR 1 cut(s) 182
BsaHI GRCGYC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 324, 579
BsaXI ACNNNNNCTCC 2 cut(s) 83, 113
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse1I ACTGG 2 cut(s) 44, 686
Bse21I CCTNAGG 1 cut(s) 270
BseDI CCNNGG 2 cut(s) 324, 579
BseGI GGATG 1 cut(s) 589
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMII CTCAG 1 cut(s) 639
BseNI ACTGG 2 cut(s) 44, 686
BseXI GCAGC 4 cut(s) 172, 175, 425, 444
Bsh1236I CGCG 1 cut(s) 708
BshFI GGCC 2 cut(s) 323, 686
BsiHKAI GWGCWC 3 cut(s) 21, 223, 482
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 3 cut(s) 30, 103, 560
BsmBI CGTCTC 1 cut(s) 103
BsmFI GGGAC 1 cut(s) 110
BsnI GGCC 2 cut(s) 323, 686
Bsp1286I GDGCHC 4 cut(s) 21, 172, 223, 482
Bsp143I GATC 4 cut(s) 10, 48, 310, 625
Bsp19I CCATGG 2 cut(s) 324, 579
BspACI CCGC 2 cut(s) 5, 508
BspANI GGCC 2 cut(s) 323, 686
BspCNI CTCAG 1 cut(s) 640
BspFNI CGCG 1 cut(s) 708
BspMAI CTGCAG 1 cut(s) 462
BspPI GGATC 3 cut(s) 43, 305, 620
BsrBI CCGCTC 1 cut(s) 510
BsrI ACTGG 2 cut(s) 44, 686
BssECI CCNNGG 2 cut(s) 324, 579
BssMI GATC 4 cut(s) 10, 48, 310, 625
BssNI GRCGYC 1 cut(s) 81
BssT1I CCWWGG 2 cut(s) 324, 579
Bst4CI ACNGT 1 cut(s) 484
BstACI GRCGYC 1 cut(s) 81
BstBAI YACGTR 1 cut(s) 182
BstC8I GCNNGC 1 cut(s) 223
BstDEI CTNAG 2 cut(s) 270, 648
BstDSI CCRYGG 2 cut(s) 324, 579
BstF5I GGATG 1 cut(s) 589
BstFNI CGCG 1 cut(s) 708
BstHHI GCGC 3 cut(s) 114, 298, 710
BstKTI GATC 4 cut(s) 13, 51, 313, 628
BstMAI GTCTC 3 cut(s) 30, 103, 560
BstMBI GATC 4 cut(s) 10, 48, 310, 625
BstSFI CTRYAG 1 cut(s) 458
BstUI CGCG 1 cut(s) 708
BstV1I GCAGC 4 cut(s) 172, 175, 425, 444
BstX2I RGATCY 1 cut(s) 10
BstYI RGATCY 1 cut(s) 10
Bsu36I CCTNAGG 1 cut(s) 270
BsuRI GGCC 2 cut(s) 323, 686
BtgI CCRYGG 2 cut(s) 324, 579
BtgZI GCGATG 1 cut(s) 689
BtsCI GGATG 1 cut(s) 589
BtsIMutI CAGTG 1 cut(s) 480
Cac8I GCNNGC 1 cut(s) 223
CaiI CAGNNNCTG 1 cut(s) 39
CfoI GCGC 3 cut(s) 114, 298, 710
Cfr13I GGNCC 1 cut(s) 684
CseI GACGC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 517
CviAII CATG 9 cut(s) 67, 109, 319, 325, 352, 381, 513, 580, 677
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 2 cut(s) 270, 648
DpnI GATC 4 cut(s) 12, 50, 312, 627
DpnII GATC 4 cut(s) 10, 48, 310, 625
EaeI YGGCCR 1 cut(s) 321
Eco130I CCWWGG 2 cut(s) 324, 579
Eco24I GRGCYC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 732
Eco81I CCTNAGG 1 cut(s) 270
EcoT14I CCWWGG 2 cut(s) 324, 579
EcoT38I GRGCYC 1 cut(s) 172
ErhI CCWWGG 2 cut(s) 324, 579
Esp3I CGTCTC 1 cut(s) 103
FaeI CATG 9 cut(s) 70, 112, 322, 328, 355, 384, 516, 583, 680
FaqI GGGAC 1 cut(s) 110
FatI CATG 9 cut(s) 66, 108, 318, 324, 351, 380, 512, 579, 676
Fnu4HI GCNGC 6 cut(s) 6, 186, 189, 414, 458, 508
FokI GGATG 1 cut(s) 596
FriOI GRGCYC 1 cut(s) 172
Fsp4HI GCNGC 6 cut(s) 6, 186, 189, 414, 458, 508
GlaI GCGC 3 cut(s) 113, 297, 709
GluI GCNGC 6 cut(s) 6, 186, 189, 414, 458, 508
HaeIII GGCC 2 cut(s) 323, 686
HgaI GACGC 1 cut(s) 70
HhaI GCGC 3 cut(s) 114, 298, 710
Hin1I GRCGYC 1 cut(s) 81
Hin1II CATG 9 cut(s) 70, 112, 322, 328, 355, 384, 516, 583, 680
Hin6I GCGC 3 cut(s) 112, 296, 708
HinP1I GCGC 3 cut(s) 112, 296, 708
HinfI GANTC 2 cut(s) 286, 367
Hpy188I TCNGA 5 cut(s) 35, 48, 89, 174, 310
Hpy188III TCNNGA 1 cut(s) 402
HpyAV CCTTC 2 cut(s) 143, 646
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4IV ACGT 2 cut(s) 181, 498
HpyCH4V TGCA 4 cut(s) 380, 413, 460, 604
HpyF3I CTNAG 2 cut(s) 270, 648
HpySE526I ACGT 2 cut(s) 181, 498
Hsp92I GRCGYC 1 cut(s) 81
Hsp92II CATG 9 cut(s) 70, 112, 322, 328, 355, 384, 516, 583, 680
HspAI GCGC 3 cut(s) 112, 296, 708
Kzo9I GATC 4 cut(s) 10, 48, 310, 625
LmnI GCTCC 2 cut(s) 119, 644
LpnPI CCDG 8 cut(s) 25, 263, 377, 387, 433, 548, 667, 686
Lsp1109I GCAGC 4 cut(s) 172, 175, 425, 444
LweI GCATC 3 cut(s) 121, 591, 712
MaeII ACGT 2 cut(s) 181, 498
MalI GATC 4 cut(s) 12, 50, 312, 627
MbiI CCGCTC 1 cut(s) 510
MboI GATC 4 cut(s) 10, 48, 310, 625
MboII GAAGA 3 cut(s) 349, 388, 581
MfeI CAATTG 1 cut(s) 155
MflI RGATCY 1 cut(s) 10
MhlI GDGCHC 4 cut(s) 21, 172, 223, 482
MlsI TGGCCA 1 cut(s) 323
MluCI AATT 7 cut(s) 155, 175, 193, 261, 388, 420, 695
MluNI TGGCCA 1 cut(s) 323
MlyI GAGTC 1 cut(s) 361
MmeI TCCRAC 1 cut(s) 141
MnlI CCTC 7 cut(s) 63, 95, 213, 238, 441, 536, 671
Mox20I TGGCCA 1 cut(s) 323
MscI TGGCCA 1 cut(s) 323
MseI TTAA 3 cut(s) 260, 525, 539
MslI CAYNNNNRTG 2 cut(s) 323, 329
Msp20I TGGCCA 1 cut(s) 323
MunI CAATTG 1 cut(s) 155
MvnI CGCG 1 cut(s) 708
NcoI CCATGG 2 cut(s) 324, 579
NdeII GATC 4 cut(s) 10, 48, 310, 625
NlaIII CATG 9 cut(s) 70, 112, 322, 328, 355, 384, 516, 583, 680
PcsI WCGNNNNNNNCGW 2 cut(s) 178, 223
PfeI GAWTC 1 cut(s) 286
PkrI GCNGC 6 cut(s) 7, 187, 190, 415, 459, 509
PleI GAGTC 1 cut(s) 361
PpsI GAGTC 1 cut(s) 361
Ppu21I YACGTR 1 cut(s) 182
PspPI GGNCC 1 cut(s) 684
PstI CTGCAG 1 cut(s) 462
PstNI CAGNNNCTG 1 cut(s) 39
PsuI RGATCY 1 cut(s) 10
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
RseI CAYNNNNRTG 2 cut(s) 323, 329
SaqAI TTAA 3 cut(s) 260, 525, 539
SatI GCNGC 6 cut(s) 6, 186, 189, 414, 458, 508
Sau3AI GATC 4 cut(s) 10, 48, 310, 625
Sau96I GGNCC 1 cut(s) 684
SchI GAGTC 1 cut(s) 361
SduI GDGCHC 4 cut(s) 21, 172, 223, 482
SetI ASST 9 cut(s) 108, 184, 205, 446, 452, 497, 501, 547, 649
SfaNI GCATC 3 cut(s) 121, 591, 712
SfcI CTRYAG 1 cut(s) 458
SmiMI CAYNNNNRTG 2 cut(s) 323, 329
SmlI CTYRAG 1 cut(s) 475
SmoI CTYRAG 1 cut(s) 475
Sse9I AATT 7 cut(s) 155, 175, 193, 261, 388, 420, 695
SsiI CCGC 2 cut(s) 5, 508
StyI CCWWGG 2 cut(s) 324, 579
TaaI ACNGT 1 cut(s) 484
TaiI ACGT 2 cut(s) 184, 501
TaqI TCGA 2 cut(s) 21, 663
TasI AATT 7 cut(s) 155, 175, 193, 261, 388, 420, 695
TauI GCSGC 2 cut(s) 8, 510
TfiI GAWTC 1 cut(s) 286
Tru1I TTAA 3 cut(s) 260, 525, 539
Tru9I TTAA 3 cut(s) 260, 525, 539
TscAI CASTG 1 cut(s) 487
TseI GCWGC 4 cut(s) 185, 188, 413, 457
TspDTI ATGAA 2 cut(s) 187, 350
TspRI CASTG 1 cut(s) 487
XapI RAATTY 3 cut(s) 175, 420, 695
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.