AT4G13070

CRS1_YhbY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006327
Physical Location & Seq
Reverse (-)
7621346 .. 7623402
2057 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G13070.1

Sequence Viewer

Length: 1032 bp
ATGTTAGCTTTAGGTTATGCCAAGGAAATCGCTCAAAGATTTCTCAGAAATCCAAAATGGACCTCTAGATTCTTCTTGCTTCACTATCAATCCTCTAATTACTCCAGTTATGTAAAGTCAAGACCTTTTCTACAAGTTCAATGTAAATCAGCTTCTTTTGATGTTTCACAAGCTCAGAGATGGAATCTATTTGCGTATGATGGTTTGTTTAGTAGACGTTACTTGAGTAATTCCACAATAGAGCTAAGAACAGATGATAATGTGGTTAGGTTTGCTTTCAACAATGTCTCTAGTAGTGGTAACAATGTTGTACCTACAAGAAAAGAGAAGAAATGGAAACGGGCTAAATTGTCAAGAAAAGCTAAAGTGAATGAGTTAAGGTTTTATCGTCTGAAGGCTAAAAAGAAGATGAATTCTCCAAACCCTGAAGTTAGAATTCGTTACAAGCTTGAAAAGGCTAAGAGGAAAGAAGAATGGTTGATTGAGAAACTGAGGAAATACGATGTTCCGAAATCACCAGCGGAGCCTTATGATCCAGAGAGTTTGACAGAGGAAGAGCAGCATTACTTAAAACGTACAGGTGAGAAGAGAAAGAACTTTGTACTTGTCGGAAGACGAGGAGTGTTTGGAGGTGTGGTTTTGAATTTGCACCTTCATTGGAAAAAGCATGAGACTGTTAAAGTTATTTGCAAGCCCTGCAACAAACCCGGGCAGGTTCATGAGTATGCAGAAGAGCTTGCTCGGTTAAGTAAGGGCATTGTGATTGATGTCAAACCTAACAACACCATTGTGTTGTACCGTGGGAAAAACTATGTGAGACCAGAAGTGATGTCTCCTGTTGATACTCTCTCTAAAGATAAGGCTTTGGAGAAATATCGGTATGAGCAATCGCTTGAACATACGAGTGAGTTCATAGAGAAGCTGGAGAAGGAGCTTGAGGAGTACCATAAGTACGTTGCTCGGCACAAGAAGAAGAAAGATGAAGAAGCAGAGAAGAAGAAAGAGGCGGTTTCGAAGAGCAAAGTAGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

343

Amino Acids

40.55

Weight (kDa)

9.87

Isoelectric Point (pI)

53.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 182 - 267 3e-19 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 703
AccI GTMKAC 1 cut(s) 214
AciI CCGC 2 cut(s) 521, 1007
AclWI GGATC 1 cut(s) 527
AcsI RAATTY 3 cut(s) 412, 435, 643
AcuI CTGAAG 2 cut(s) 413, 447
AfaI GTAC 6 cut(s) 312, 577, 603, 797, 944, 953
AgsI TTSAA 5 cut(s) 140, 280, 452, 643, 896
AleI CACNNNNGTG 1 cut(s) 788
AluBI AGCT 9 cut(s) 8, 152, 173, 244, 362, 448, 736, 922, 934
AluI AGCT 9 cut(s) 8, 152, 173, 244, 362, 448, 736, 922, 934
Alw26I GTCTC 4 cut(s) 292, 665, 811, 837
AlwI GGATC 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 707
ApeKI GCWGC 1 cut(s) 559
ApoI RAATTY 3 cut(s) 412, 435, 643
AspS9I GGNCC 1 cut(s) 60
AsuC2I CCSGG 2 cut(s) 708, 709
AsuHPI GGTGA 2 cut(s) 507, 593
AsuII TTCGAA 1 cut(s) 1013
AvaI CYCGRG 1 cut(s) 707
AvaII GGWCC 1 cut(s) 60
BarI GAAGNNNNNNTAC 2 cut(s) 136, 168
BbsI GAAGAC 1 cut(s) 619
BbvI GCAGC 1 cut(s) 571
BccI CCATC 2 cut(s) 174, 194
BcnI CCSGG 2 cut(s) 708, 709
BcoDI GTCTC 4 cut(s) 292, 665, 811, 837
BfaI CTAG 2 cut(s) 66, 291
BfuAI ACCTGC 1 cut(s) 703
BisI GCNGC 1 cut(s) 560
BlsI GCNGC 1 cut(s) 561
Bme1390I CCNGG 2 cut(s) 708, 709
Bme18I GGWCC 1 cut(s) 60
BmeT110I CYCGRG 1 cut(s) 707
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 1 cut(s) 525
BmrFI CCNGG 2 cut(s) 708, 709
BpiI GAAGAC 1 cut(s) 619
BpmI CTGGAG 2 cut(s) 88, 944
Bpu14I TTCGAA 1 cut(s) 1013
BpuEI CTTGAG 2 cut(s) 244, 956
BpuMI CCSGG 2 cut(s) 708, 709
BsaI GGTCTC 1 cut(s) 811
BsaJI CCNNGG 3 cut(s) 21, 707, 799
BsaXI ACNNNNNCTCC 2 cut(s) 621, 651
Bse1I ACTGG 1 cut(s) 105
BseDI CCNNGG 3 cut(s) 21, 707, 799
BseMII CTCAG 3 cut(s) 58, 188, 482
BseNI ACTGG 1 cut(s) 105
BseRI GAGGAG 2 cut(s) 633, 953
BseXI GCAGC 1 cut(s) 571
BsiHKCI CYCGRG 1 cut(s) 707
BsiSI CCGG 1 cut(s) 708
BsmAI GTCTC 4 cut(s) 292, 665, 811, 837
Bso31I GGTCTC 1 cut(s) 811
BsoBI CYCGRG 1 cut(s) 707
Bsp119I TTCGAA 1 cut(s) 1013
Bsp143I GATC 1 cut(s) 532
BspACI CCGC 2 cut(s) 521, 1007
BspCNI CTCAG 3 cut(s) 57, 187, 483
BspHI TCATGA 1 cut(s) 718
BspLI GGNNCC 1 cut(s) 525
BspMI ACCTGC 1 cut(s) 703
BspPI GGATC 1 cut(s) 527
BspQI GCTCTTC 3 cut(s) 549, 726, 1010
BspT104I TTCGAA 1 cut(s) 1013
BspTNI GGTCTC 1 cut(s) 811
BsrI ACTGG 1 cut(s) 105
BssECI CCNNGG 3 cut(s) 21, 707, 799
BssMI GATC 1 cut(s) 532
BssT1I CCWWGG 1 cut(s) 21
Bst4CI ACNGT 2 cut(s) 676, 800
Bst6I CTCTTC 4 cut(s) 549, 581, 726, 1010
BstAPI GCANNNNNTGC 1 cut(s) 696
BstBI TTCGAA 1 cut(s) 1013
BstC8I GCNNGC 2 cut(s) 692, 738
BstDEI CTNAG 5 cut(s) 44, 174, 245, 459, 491
BstDSI CCRYGG 1 cut(s) 799
BstKTI GATC 1 cut(s) 535
BstMAI GTCTC 4 cut(s) 292, 665, 811, 837
BstMBI GATC 1 cut(s) 532
BstMWI GCNNNNNNNGC 1 cut(s) 696
BstSCI CCNGG 2 cut(s) 706, 707
BstV1I GCAGC 1 cut(s) 571
BstV2I GAAGAC 1 cut(s) 619
BtgI CCRYGG 1 cut(s) 799
BveI ACCTGC 1 cut(s) 703
Cac8I GCNNGC 2 cut(s) 692, 738
CciI TCATGA 1 cut(s) 718
Cfr13I GGNCC 1 cut(s) 60
Cfr9I CCCGGG 1 cut(s) 707
Csp6I GTAC 6 cut(s) 311, 576, 602, 796, 943, 952
CviAII CATG 2 cut(s) 668, 719
CviQI GTAC 6 cut(s) 311, 576, 602, 796, 943, 952
DdeI CTNAG 5 cut(s) 44, 174, 245, 459, 491
DpnI GATC 1 cut(s) 534
DpnII GATC 1 cut(s) 532
Eam1104I CTCTTC 4 cut(s) 549, 581, 726, 1010
EarI CTCTTC 4 cut(s) 549, 581, 726, 1010
Eco130I CCWWGG 1 cut(s) 21
Eco31I GGTCTC 1 cut(s) 811
Eco47I GGWCC 1 cut(s) 60
Eco57I CTGAAG 2 cut(s) 413, 447
Eco88I CYCGRG 1 cut(s) 707
EcoRI GAATTC 2 cut(s) 412, 435
EcoT14I CCWWGG 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 2 cut(s) 671, 722
FatI CATG 2 cut(s) 667, 718
FblI GTMKAC 1 cut(s) 214
Fnu4HI GCNGC 1 cut(s) 560
Fsp4HI GCNGC 1 cut(s) 560
FspBI CTAG 2 cut(s) 66, 291
GluI GCNGC 1 cut(s) 560
GsuI CTGGAG 2 cut(s) 88, 944
HapII CCGG 1 cut(s) 708
Hin1II CATG 2 cut(s) 671, 722
HindIII AAGCTT 1 cut(s) 446
HinfI GANTC 2 cut(s) 69, 184
HpaII CCGG 1 cut(s) 708
HphI GGTGA 2 cut(s) 507, 593
Hpy166II GTNNAC 1 cut(s) 215
Hpy188I TCNGA 5 cut(s) 47, 177, 393, 510, 611
Hpy188III TCNNGA 5 cut(s) 66, 120, 354, 536, 719
Hpy8I GTNNAC 1 cut(s) 215
HpyAV CCTTC 3 cut(s) 388, 662, 922
HpyCH4III ACNGT 2 cut(s) 676, 800
HpyCH4IV ACGT 3 cut(s) 217, 574, 954
HpyCH4V TGCA 4 cut(s) 649, 690, 699, 728
HpyF10VI GCNNNNNNNGC 1 cut(s) 696
HpyF3I CTNAG 5 cut(s) 44, 174, 245, 459, 491
HpySE526I ACGT 3 cut(s) 217, 574, 954
Hsp92II CATG 2 cut(s) 671, 722
Kzo9I GATC 1 cut(s) 532
LguI GCTCTTC 3 cut(s) 549, 726, 1010
LmnI GCTCC 2 cut(s) 523, 931
Lsp1109I GCAGC 1 cut(s) 571
MaeI CTAG 2 cut(s) 66, 291
MaeII ACGT 3 cut(s) 217, 574, 954
MaeIII GTNAC 3 cut(s) 218, 299, 440
MalI GATC 1 cut(s) 534
MboI GATC 1 cut(s) 532
MluCI AATT 6 cut(s) 97, 229, 347, 412, 435, 643
MmeI TCCRAC 1 cut(s) 589
MnlI CCTC 9 cut(s) 73, 103, 456, 486, 544, 611, 623, 931, 997
MseI TTAA 4 cut(s) 377, 569, 678, 746
MslI CAYNNNNRTG 3 cut(s) 723, 788, 903
MspA1I CMGCKG 1 cut(s) 521
MspI CCGG 1 cut(s) 708
MspR9I CCNGG 2 cut(s) 708, 709
MwoI GCNNNNNNNGC 1 cut(s) 696
NciI CCSGG 2 cut(s) 708, 709
NdeII GATC 1 cut(s) 532
NlaIII CATG 2 cut(s) 671, 722
NlaIV GGNNCC 1 cut(s) 525
NmeAIII GCCGAG 1 cut(s) 940
NspV TTCGAA 1 cut(s) 1013
OliI CACNNNNGTG 1 cut(s) 788
PagI TCATGA 1 cut(s) 718
PciSI GCTCTTC 3 cut(s) 549, 726, 1010
PfeI GAWTC 2 cut(s) 69, 184
PkrI GCNGC 1 cut(s) 561
PspN4I GGNNCC 1 cut(s) 525
PspPI GGNCC 1 cut(s) 60
RsaI GTAC 6 cut(s) 312, 577, 603, 797, 944, 953
RsaNI GTAC 6 cut(s) 311, 576, 602, 796, 943, 952
RseI CAYNNNNRTG 3 cut(s) 723, 788, 903
SapI GCTCTTC 3 cut(s) 549, 726, 1010
SaqAI TTAA 4 cut(s) 377, 569, 678, 746
SatI GCNGC 1 cut(s) 560
Sau3AI GATC 1 cut(s) 532
Sau96I GGNCC 1 cut(s) 60
ScrFI CCNGG 2 cut(s) 708, 709
SfuI TTCGAA 1 cut(s) 1013
SinI GGWCC 1 cut(s) 60
SmaI CCCGGG 1 cut(s) 709
SmiMI CAYNNNNRTG 3 cut(s) 723, 788, 903
SmlI CTYRAG 2 cut(s) 223, 935
SmoI CTYRAG 2 cut(s) 223, 935
Sse9I AATT 6 cut(s) 97, 229, 347, 412, 435, 643
SsiI CCGC 2 cut(s) 521, 1007
SspMI CTAG 2 cut(s) 66, 291
StyD4I CCNGG 2 cut(s) 706, 707
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 2 cut(s) 676, 800
TaiI ACGT 3 cut(s) 220, 577, 957
TaqI TCGA 1 cut(s) 1013
TasI AATT 6 cut(s) 97, 229, 347, 412, 435, 643
TatI WGTACW 1 cut(s) 601
TfiI GAWTC 2 cut(s) 69, 184
Tru1I TTAA 4 cut(s) 377, 569, 678, 746
Tru9I TTAA 4 cut(s) 377, 569, 678, 746
TseI GCWGC 1 cut(s) 559
TspDTI ATGAA 5 cut(s) 425, 644, 707, 901, 996
TspMI CCCGGG 1 cut(s) 707
VpaK11BI GGWCC 1 cut(s) 60
XapI RAATTY 3 cut(s) 412, 435, 643
XbaI TCTAGA 1 cut(s) 65
XmaI CCCGGG 1 cut(s) 707
XmiI GTMKAC 1 cut(s) 214
XspI CTAG 2 cut(s) 66, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.