MD10G1049100.v1.1

CRM domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0006327
Physical Location & Seq
Forward (+)
6527825 .. 6534482
6658 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1049100.v1.1.491

Sequence Viewer

Length: 1104 bp
ATGTTAGCTTCTTCAGCTTCAGCTAGAGCCGTTTTTCGCTCTTGCCCAAATGCAATCAAAAGGGACCCTTTTCTCTGGTTGTATTGTGTAACCCAAAACGTTTTCGATCACTCGTTGCCATCCACAACATGCTTTACACACTCTCCTTCCGAAGCTTCGTGTACATCCTCTGCATTGGACTCATTATCCGTGCGGAGGTCAAATGTTTTTACGTCTCATAGCATTCTTCGAAACTGCCTGCCTGCTACTGCTAGATGCATGGGCGGTACATCATTCGAGGTGAAGACAGACAATGATGTTGTCAGGTTTTCAATCGGCAAGCCACTCGATAAGATTGGTCCCTCAAACAATGGGAACAAGAAGATGGCTGTAACCCGACGTGTTAAAATGTCCAAGAAGGCCAAACTAAATGAACTGCGATTTTACCGCCTCAAGGCCAAAAAGAAGATGAACTCTCCAAATCCACAAGTTAGAATTATATACAAACTTGAAAAGGCAAAACGAAAGGAAGCATGGTTGATTGAGAAGTTGAGGAAATTTGAGGTTCCCAAAGCCCCCGCTGAAACACATGATCCTGAAATTTTAACTGAGGAAGAAAAACATTATCTTAAGCGAACCGGTGAGAAAAAGAAGAACTATGTCCCAGTAGGAAGGCGAGGAGTATTCGGAGGGGTGGTTTTAAATATGCATCTGCATTGGAAGAAGCATGAGACTGTTAAGGTCATTTGCAAGCCTTGTAAGCCAGGACAGGTTCATGAATATGCTGAAGAGCTTGCTCGACTGAGTAAAGGCATTGTGATTGACATAAAACCTAATAATAGCATAATTTTCTACCGGGGAAAGAACTATGTGCGGCCGAAAGTAATGTCACCTCCAGATACATTATCCAAAAATAAGGCACTGGAAAAATATAAATACGAGCAGTCCCTCGAACATACAAGTCAGTTCATTGAAAAGCTTGAGAATGAACTAGAAGAGTATCAGAAGCACGTTGCTCGATTTAAGAAAGCAAATGAGGATGCAACACAAGAATCTTTGCAAACGTTTATGAAGCTGGTGTTGGAGGTGTGTCAGGTTCTGCATTTCAATCTCTTTGAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

368

Amino Acids

42.17

Weight (kDa)

9.72

Isoelectric Point (pI)

54.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 195 - 279 6.2e-19 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 193, 264, 427, 558, 853
AclI AACGTT 2 cut(s) 99, 1043
AclWI GGATC 1 cut(s) 566
AcoI YGGCCR 1 cut(s) 854
AcsI RAATTY 2 cut(s) 536, 579
AcuI CTGAAG 1 cut(s) 786
AfaI GTAC 2 cut(s) 163, 268
AfiI CCNNNNNNNGG 2 cut(s) 195, 433
AflII CTTAAG 1 cut(s) 608
AflIII ACRYGT 1 cut(s) 379
AgeI ACCGGT 1 cut(s) 617
AgsI TTSAA 5 cut(s) 312, 491, 953, 1087, 1097
AjiI CACGTC 1 cut(s) 380
AjnI CCWGG 1 cut(s) 742
AjuI GAANNNNNNNTTGG 2 cut(s) 1043, 1075
AluBI AGCT 7 cut(s) 8, 17, 23, 155, 772, 958, 1054
AluI AGCT 7 cut(s) 8, 17, 23, 155, 772, 958, 1054
Alw26I GTCTC 2 cut(s) 219, 704
AlwI GGATC 1 cut(s) 566
AlwNI CAGNNNCTG 1 cut(s) 1078
AoxI GGCC 3 cut(s) 399, 435, 854
ApoI RAATTY 2 cut(s) 536, 579
ArsI GACNNNNNNTTYG 2 cut(s) 851, 883
AsiGI ACCGGT 1 cut(s) 617
AspS9I GGNCC 2 cut(s) 64, 338
AsuC2I CCSGG 1 cut(s) 836
AsuHPI GGTGA 3 cut(s) 292, 632, 861
AsuII TTCGAA 1 cut(s) 229
AvaII GGWCC 2 cut(s) 64, 338
BbsI GAAGAC 1 cut(s) 290
BccI CCATC 2 cut(s) 127, 358
BceAI ACGGC 1 cut(s) 14
BcgI CGANNNNNNTGC 4 cut(s) 307, 341, 977, 1011
BciT130I CCWGG 1 cut(s) 744
BcnI CCSGG 1 cut(s) 836
BcoDI GTCTC 2 cut(s) 219, 704
BfaI CTAG 3 cut(s) 24, 252, 971
BfrI CTTAAG 1 cut(s) 608
BisI GCNGC 1 cut(s) 854
BlsI GCNGC 1 cut(s) 855
Bme1390I CCNGG 2 cut(s) 744, 836
Bme18I GGWCC 2 cut(s) 64, 338
BmgBI CACGTC 1 cut(s) 380
BmgT120I GGNCC 2 cut(s) 64, 338
BmiI GGNNCC 4 cut(s) 65, 66, 340, 546
BmrFI CCNGG 2 cut(s) 744, 836
BmrI ACTGGG 1 cut(s) 638
BmsI GCATC 3 cut(s) 245, 697, 1009
BmuI ACTGGG 1 cut(s) 638
BpiI GAAGAC 1 cut(s) 290
BpmI CTGGAG 1 cut(s) 858
Bpu14I TTCGAA 1 cut(s) 229
BpuEI CTTGAG 2 cut(s) 416, 980
BpuMI CCSGG 1 cut(s) 836
BsaJI CCNNGG 1 cut(s) 835
BsaWI WCCGGW 1 cut(s) 617
BsaXI ACNNNNNCTCC 4 cut(s) 127, 157, 660, 690
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 433
Bse118I RCCGGY 1 cut(s) 617
Bse1I ACTGG 2 cut(s) 644, 906
BseBI CCWGG 1 cut(s) 744
BseDI CCNNGG 1 cut(s) 835
BseGI GGATG 3 cut(s) 119, 164, 1024
BseLI CCNNNNNNNGG 2 cut(s) 195, 433
BseMII CTCAG 2 cut(s) 579, 773
BseNI ACTGG 2 cut(s) 644, 906
BseRI GAGGAG 1 cut(s) 672
BseX3I CGGCCG 1 cut(s) 854
Bsh1285I CGRYCG 1 cut(s) 857
BshFI GGCC 3 cut(s) 401, 437, 856
BshTI ACCGGT 1 cut(s) 617
BsiEI CGRYCG 1 cut(s) 857
BsiSI CCGG 2 cut(s) 618, 835
BslFI GGGAC 4 cut(s) 77, 324, 626, 910
BslI CCNNNNNNNGG 2 cut(s) 195, 433
BsmAI GTCTC 2 cut(s) 219, 704
BsmBI CGTCTC 1 cut(s) 219
BsmFI GGGAC 4 cut(s) 77, 324, 626, 910
BsmI GAATGC 1 cut(s) 222
BsnI GGCC 3 cut(s) 401, 437, 856
Bsp119I TTCGAA 1 cut(s) 229
Bsp1407I TGTACA 1 cut(s) 161
Bsp143I GATC 2 cut(s) 106, 571
BspACI CCGC 5 cut(s) 193, 264, 427, 558, 853
BspANI GGCC 3 cut(s) 401, 437, 856
BspCNI CTCAG 2 cut(s) 580, 774
BspHI TCATGA 1 cut(s) 754
BspLI GGNNCC 4 cut(s) 65, 66, 340, 546
BspPI GGATC 1 cut(s) 566
BspQI GCTCTTC 1 cut(s) 762
BspT104I TTCGAA 1 cut(s) 229
BspTI CTTAAG 1 cut(s) 608
BsrFI RCCGGY 1 cut(s) 617
BsrGI TGTACA 1 cut(s) 161
BsrI ACTGG 2 cut(s) 644, 906
BssAI RCCGGY 1 cut(s) 617
BssECI CCNNGG 1 cut(s) 835
BssMI GATC 2 cut(s) 106, 571
Bst2UI CCWGG 1 cut(s) 744
Bst4CI ACNGT 1 cut(s) 715
Bst6I CTCTTC 2 cut(s) 762, 969
BstAFI CTTAAG 1 cut(s) 608
BstAUI TGTACA 1 cut(s) 161
BstBI TTCGAA 1 cut(s) 229
BstC8I GCNNGC 5 cut(s) 239, 243, 320, 731, 774
BstDEI CTNAG 2 cut(s) 588, 782
BstF5I GGATG 3 cut(s) 119, 164, 1024
BstKTI GATC 2 cut(s) 109, 574
BstMAI GTCTC 2 cut(s) 219, 704
BstMBI GATC 2 cut(s) 106, 571
BstMCI CGRYCG 1 cut(s) 857
BstMWI GCNNNNNNNGC 2 cut(s) 14, 739
BstNI CCWGG 1 cut(s) 744
BstNSI RCATGY 1 cut(s) 132
BstSCI CCNGG 2 cut(s) 742, 834
BstV2I GAAGAC 1 cut(s) 290
BstZI CGGCCG 1 cut(s) 854
BsuRI GGCC 3 cut(s) 401, 437, 856
BtrI CACGTC 1 cut(s) 380
BtsCI GGATG 3 cut(s) 119, 164, 1024
BtsIMutI CAGTG 1 cut(s) 899
Cac8I GCNNGC 5 cut(s) 239, 243, 320, 731, 774
CaiI CAGNNNCTG 1 cut(s) 1078
CciI TCATGA 1 cut(s) 754
Cfr10I RCCGGY 1 cut(s) 617
Cfr13I GGNCC 2 cut(s) 64, 338
Csp6I GTAC 2 cut(s) 162, 267
CspAI ACCGGT 1 cut(s) 617
CviAII CATG 6 cut(s) 129, 259, 513, 569, 707, 755
CviQI GTAC 2 cut(s) 162, 267
DdeI CTNAG 2 cut(s) 588, 782
DpnI GATC 2 cut(s) 108, 573
DpnII GATC 2 cut(s) 106, 571
DraI TTTAAA 1 cut(s) 681
EaeI YGGCCR 1 cut(s) 854
EagI CGGCCG 1 cut(s) 854
Eam1104I CTCTTC 2 cut(s) 762, 969
EarI CTCTTC 2 cut(s) 762, 969
EclXI CGGCCG 1 cut(s) 854
Eco47I GGWCC 2 cut(s) 64, 338
Eco52I CGGCCG 1 cut(s) 854
Eco57I CTGAAG 1 cut(s) 786
EcoO109I RGGNCCY 1 cut(s) 64
EcoRII CCWGG 1 cut(s) 742
EcoT22I ATGCAT 2 cut(s) 260, 690
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 6 cut(s) 132, 262, 516, 572, 710, 758
FalI AAGNNNNNCTT 2 cut(s) 52, 84
FaqI GGGAC 4 cut(s) 77, 324, 626, 910
FatI CATG 6 cut(s) 128, 258, 512, 568, 706, 754
FauI CCCGC 1 cut(s) 565
Fnu4HI GCNGC 1 cut(s) 854
FokI GGATG 3 cut(s) 106, 151, 1031
Fsp4HI GCNGC 1 cut(s) 854
FspBI CTAG 3 cut(s) 24, 252, 971
GluI GCNGC 1 cut(s) 854
GsuI CTGGAG 1 cut(s) 858
HaeIII GGCC 3 cut(s) 401, 437, 856
HapII CCGG 2 cut(s) 618, 835
Hin1II CATG 6 cut(s) 132, 262, 516, 572, 710, 758
HindIII AAGCTT 2 cut(s) 153, 956
HinfI GANTC 2 cut(s) 179, 1031
HpaII CCGG 2 cut(s) 618, 835
HphI GGTGA 3 cut(s) 292, 632, 861
Hpy166II GTNNAC 1 cut(s) 162
Hpy188I TCNGA 3 cut(s) 151, 668, 984
Hpy188III TCNNGA 3 cut(s) 575, 755, 875
Hpy8I GTNNAC 1 cut(s) 162
Hpy99I CGWCG 1 cut(s) 381
HpyAV CCTTC 3 cut(s) 156, 391, 645
HpyCH4III ACNGT 1 cut(s) 715
HpyCH4IV ACGT 5 cut(s) 99, 212, 379, 990, 1043
HpyCH4V TGCA 9 cut(s) 53, 173, 258, 688, 694, 729, 1022, 1039, 1081
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 739
HpyF3I CTNAG 2 cut(s) 588, 782
HpySE526I ACGT 5 cut(s) 99, 212, 379, 990, 1043
Hsp92II CATG 6 cut(s) 132, 262, 516, 572, 710, 758
KflI GGGWCCC 1 cut(s) 64
Kzo9I GATC 2 cut(s) 106, 571
LguI GCTCTTC 1 cut(s) 762
LweI GCATC 3 cut(s) 245, 697, 1009
MaeI CTAG 3 cut(s) 24, 252, 971
MaeII ACGT 5 cut(s) 99, 212, 379, 990, 1043
MaeIII GTNAC 3 cut(s) 88, 370, 867
MalI GATC 2 cut(s) 108, 573
MboI GATC 2 cut(s) 106, 571
MluCI AATT 4 cut(s) 474, 536, 579, 825
MlyI GAGTC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 1041
Mph1103I ATGCAT 2 cut(s) 260, 690
MseI TTAA 6 cut(s) 384, 584, 609, 680, 717, 1002
MslI CAYNNNNRTG 1 cut(s) 759
MspA1I CMGCKG 1 cut(s) 560
MspCI CTTAAG 1 cut(s) 608
MspI CCGG 2 cut(s) 618, 835
MspR9I CCNGG 2 cut(s) 744, 836
Mva1269I GAATGC 1 cut(s) 222
MvaI CCWGG 1 cut(s) 744
MwoI GCNNNNNNNGC 2 cut(s) 14, 739
NciI CCSGG 1 cut(s) 836
NdeII GATC 2 cut(s) 106, 571
NlaIII CATG 6 cut(s) 132, 262, 516, 572, 710, 758
NlaIV GGNNCC 4 cut(s) 65, 66, 340, 546
NmuCI GTSAC 1 cut(s) 867
NsiI ATGCAT 2 cut(s) 260, 690
NspI RCATGY 1 cut(s) 132
NspV TTCGAA 1 cut(s) 229
PagI TCATGA 1 cut(s) 754
PciSI GCTCTTC 1 cut(s) 762
PctI GAATGC 1 cut(s) 222
PfeI GAWTC 1 cut(s) 1031
PinAI ACCGGT 1 cut(s) 617
PkrI GCNGC 1 cut(s) 855
PleI GAGTC 1 cut(s) 173
PpsI GAGTC 1 cut(s) 173
PpuMI RGGWCCY 1 cut(s) 64
Psp1406I AACGTT 2 cut(s) 99, 1043
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 742
PspGI CCWGG 1 cut(s) 742
PspN4I GGNNCC 4 cut(s) 65, 66, 340, 546
PspPI GGNCC 2 cut(s) 64, 338
PspPPI RGGWCCY 1 cut(s) 64
PstNI CAGNNNCTG 1 cut(s) 1078
RsaI GTAC 2 cut(s) 163, 268
RsaNI GTAC 2 cut(s) 162, 267
RseI CAYNNNNRTG 1 cut(s) 759
SapI GCTCTTC 1 cut(s) 762
SaqAI TTAA 6 cut(s) 384, 584, 609, 680, 717, 1002
SatI GCNGC 1 cut(s) 854
Sau3AI GATC 2 cut(s) 106, 571
Sau96I GGNCC 2 cut(s) 64, 338
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 2 cut(s) 744, 836
SfaNI GCATC 3 cut(s) 245, 697, 1009
SfuI TTCGAA 1 cut(s) 229
SinI GGWCC 2 cut(s) 64, 338
SmiMI CAYNNNNRTG 1 cut(s) 759
SmlI CTYRAG 3 cut(s) 431, 608, 959
SmoI CTYRAG 3 cut(s) 431, 608, 959
Sse9I AATT 4 cut(s) 474, 536, 579, 825
SsiI CCGC 5 cut(s) 193, 264, 427, 558, 853
SspMI CTAG 3 cut(s) 24, 252, 971
StyD4I CCNGG 2 cut(s) 742, 834
TaaI ACNGT 1 cut(s) 715
TaiI ACGT 5 cut(s) 102, 215, 382, 993, 1046
TaqI TCGA 7 cut(s) 105, 229, 276, 327, 778, 930, 997
TasI AATT 4 cut(s) 474, 536, 579, 825
TatI WGTACW 1 cut(s) 161
TauI GCSGC 1 cut(s) 856
TfiI GAWTC 1 cut(s) 1031
Tru1I TTAA 6 cut(s) 384, 584, 609, 680, 717, 1002
Tru9I TTAA 6 cut(s) 384, 584, 609, 680, 717, 1002
TscAI CASTG 1 cut(s) 906
TseFI GTSAC 1 cut(s) 867
Tsp45I GTSAC 1 cut(s) 867
TspDTI ATGAA 7 cut(s) 426, 464, 743, 771, 937, 981, 1064
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 1 cut(s) 906
Vha464I CTTAAG 1 cut(s) 608
VpaK11BI GGWCC 2 cut(s) 64, 338
XapI RAATTY 2 cut(s) 536, 579
XceI RCATGY 1 cut(s) 132
XspI CTAG 3 cut(s) 24, 252, 971
Zsp2I ATGCAT 2 cut(s) 260, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.