Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3C

Orthogroup ID

OG0006327

Physical Location & Seq

Genomic Coordinates Forward (+)

36884618 .. 36891016

Gene Structure 6399 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3CG331800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1377 bp

ATGGCCAAAACCAGGGTCACCCATCTGCCCAAAGAGAAAAAAAAAAAAAAAAATTTATCTGCAACCAAGCTTGCTTTGTTTTCTGATTTGCTCCAAACGGGAGATTTGGGACACAGCAGGACAAGAAAGGTTTCAAAGTCTCAGGGCAGCATTTTATGTGGAGCAGATTGTTGTGTTCTTGTTTATGATGTGAATGTTCTAAGATCATTTGAAACACTTCAGAACTGGCATGAAGAGTTTCTCAAACAGCAGGTAGGTCCAGCTGACACTGAGGCATTTCCATTTATACTACTCGGCAACAAAATTGATATAGATGGCGGAAACAGCAGAGCGACTCTGGCTGTGGCTGGATTTTCAAGGATGGGCATCGCAGGAGGAACAAGGAATTCGTTGCTCCGCTTCTGTTGTTTGGCCAAAAATGTGTTAGGCCTCTCTCTGCCAACCATAGGCTCTCTTCCTTGTCACATGTGTATACAAAGGCAAAGCTACCGGGTTATGGCATCTTTCATACTCCCAGTAGATTCTGCATTGCTGGGTCGATCAAATATGTATAAGTATCAAACTCTTCTCAGAAATTTGCCACCCACCAGATGCATGAGTAGGACTTCGTTTGAGCTCAACACAGACAATGGTGTTGTGCGGTTTTCAATTGGTAAGCCACTTGATGAAACTAGTTCCTTGACACAAGGGAAGAAGGTGGCCAAACAAGTTAAAATGTCCAAGAAGGCCAAACTGAATGAACTACGATTTTATCGTCTAAAGGCCAAAAAGAAGATCAATTCTCCAAATCCGGAAGTTAGGATTAGATACAAACTGGAAAAGGCAAAACGAAAGGAAGCATGGTTGATTGAGAAGTTGAGAAAATTCCAGGTTCACAAAGCTTCTGCTGAAACACATGATCCTGAAATTTTAACTGAAGAAGAAAAGCATTATCTTAAGCGAACTGGTGAGAAAAAGAAGCACTATGTGCCAGTTGGAAGGCGGGGAGTATTTGGAGGGGTTGTTTTAAATATGCATCTGCATTGGAAGAAGCACGAGACAGTAAAGGTTATATGCAAACCTTGTAAGCCAGGACAGGTTCATGAATATGCTGATGAGCTAGCTCGGCTGAGCAAAGGCATAGTGATTGACATCAAACCTAATAATACTGTAATTTTCTATCGGGGGAAGAACTATGTGCAGCCGGAAGTAATGTCACCACCAGATACACTGTCCAAAAATAAGGCGCTCGAAAAATATAGGTATGAGCAGTCCCTCGAACATACAGGTCAGTTTATTGAAAAATTGGAGAAAGAACTAGAGGAATATCACCAGCATCTTGCTCGGTTTAAGAAAGTAAAAGAGGATACGACACAAGATTGTTTGATCTATAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRS1_YhbY

Protein Analysis

458

Amino Acids

52.34

Weight (kDa)

9.77

Isoelectric Point (pI)

44.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ras	PF00071	50 - 105	1.2e-07	Ras family
CRS1_YhbY	PF01985	304 - 388	1.8e-18	CRS1 / YhbY (CRM) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006327)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13070
fragaria_vesca	FvH4_1g30180 FvH4_1g30180 FvH4_1g30180 FvH4_1g30180 FvH4_1g30180 FvH4_1g30180
malus_domestica	MD10G1049100.v1.1
prunus_persica	Prupe.7G001900_v2.0.a1 Prupe.7G001900_v2.0.a1 Prupe.7G001900_v2.0.a1 Prupe.7G001900_v2.0.a1 Prupe.7G001900_v2.0.a1 Prupe.7G001900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0491551
rosa_laevigata	RLG00000022837 RLG00000022839
rosa_multiflora	Rmu_sc0001267.1_g000015
rosa_roxburghii	Rroxscaffold_6G00392910 Rroxscaffold_6G00392940
rosa_rugosa	Rorug03G0250200
rosa_samantha	Rh3AG299200 Rh3BG335000 Rh3BG335600 Rh3CG331800 Rh3DG333500
rosa_wichuraiana	Rw3G026420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	241
AccI	GTMKAC	1 cut(s)	472
AccIII	TCCGGA	1 cut(s)	790
AciI	CCGC	4 cut(s)	318, 397, 640, 982
AclWI	GGATC	1 cut(s)	893
AcoI	YGGCCR	3 cut(s)	3, 411, 699
AcsI	RAATTY	5 cut(s)	52, 385, 574, 863, 906
AcuI	CTGAAG	2 cut(s)	203, 936
AdeI	CACNNNGTG	1 cut(s)	967
AfiI	CCNNNNNNNGG	3 cut(s)	12, 446, 496
AflII	CTTAAG	1 cut(s)	935
AflIII	ACRYGT	1 cut(s)	465
AgsI	TTSAA	5 cut(s)	135, 212, 357, 648, 1280
AhlI	ACTAGT	1 cut(s)	671
AjnI	CCWGG	3 cut(s)	11, 867, 1069
AluBI	AGCT	7 cut(s)	70, 263, 486, 616, 881, 1099, 1103
AluI	AGCT	7 cut(s)	70, 263, 486, 616, 881, 1099, 1103
Alw21I	GWGCWC	1 cut(s)	618
Alw26I	GTCTC	2 cut(s)	144, 1031
AlwI	GGATC	1 cut(s)	893
Aor13HI	TCCGGA	1 cut(s)	790
AoxI	GGCC	6 cut(s)	3, 411, 427, 699, 726, 762
ApeKI	GCWGC	2 cut(s)	147, 1180
ApoI	RAATTY	5 cut(s)	52, 385, 574, 863, 906
ArsI	GACNNNNNNTTYG	1 cut(s)	32
Asp700I	GAANNNNTTC	3 cut(s)	130, 216, 237
AspLEI	GCGC	1 cut(s)	1228
AspS9I	GGNCC	1 cut(s)	257
AsuC2I	CCSGG	1 cut(s)	491
AsuHPI	GGTGA	4 cut(s)	10, 959, 1188, 1301
AsuNHI	GCTAGC	1 cut(s)	1099
AvaII	GGWCC	1 cut(s)	257
BalI	TGGCCA	3 cut(s)	5, 413, 701
BanII	GRGCYC	1 cut(s)	618
BauI	CACGAG	1 cut(s)	1034
Bbv12I	GWGCWC	1 cut(s)	618
BbvI	GCAGC	2 cut(s)	159, 1192
BccI	CCATC	3 cut(s)	30, 308, 355
BcgI	CGANNNNNNTGC	2 cut(s)	1304, 1338
BciT130I	CCWGG	3 cut(s)	13, 869, 1071
BciVI	GTATCC	1 cut(s)	1339
BcnI	CCSGG	1 cut(s)	491
BcoDI	GTCTC	2 cut(s)	144, 1031
BcuI	ACTAGT	1 cut(s)	671
BfaI	CTAG	3 cut(s)	672, 1100, 1298
BfmI	CTRYAG	1 cut(s)	1369
BfoI	RGCGCY	1 cut(s)	1229
BfrI	CTTAAG	1 cut(s)	935
BfuAI	ACCTGC	1 cut(s)	241
BfuI	GTATCC	1 cut(s)	1339
BisI	GCNGC	2 cut(s)	148, 1181
BlpI	GCTNAGC	1 cut(s)	1109
BlsI	GCNGC	2 cut(s)	149, 1182
Bme1390I	CCNGG	4 cut(s)	13, 491, 869, 1071
Bme18I	GGWCC	1 cut(s)	257
BmgT120I	GGNCC	1 cut(s)	257
BmrFI	CCNGG	4 cut(s)	13, 491, 869, 1071
BmrI	ACTGGG	1 cut(s)	509
BmsI	GCATC	5 cut(s)	375, 509, 581, 1024, 1324
BmtI	GCTAGC	1 cut(s)	1103
BmuI	ACTGGG	1 cut(s)	509
Bpu1102I	GCTNAGC	1 cut(s)	1109
BpuMI	CCSGG	1 cut(s)	491
BsaBI	GATNNNNATC	2 cut(s)	365, 1130
BsaJI	CCNNGG	1 cut(s)	12
BsaWI	WCCGGW	1 cut(s)	790
BsaXI	ACNNNNNCTCC	2 cut(s)	987, 1017
Bsc4I	CCNNNNNNNGG	3 cut(s)	12, 446, 496
Bse1I	ACTGG	5 cut(s)	230, 515, 819, 949, 971
Bse3DI	GCAATG	1 cut(s)	527
Bse8I	GATNNNNATC	2 cut(s)	365, 1130
BseAI	TCCGGA	1 cut(s)	790
BseBI	CCWGG	3 cut(s)	13, 869, 1071
BseDI	CCNNGG	1 cut(s)	12
BseGI	GGATG	1 cut(s)	366
BseJI	GATNNNNATC	2 cut(s)	365, 1130
BseLI	CCNNNNNNNGG	3 cut(s)	12, 446, 496
BseMI	GCAATG	1 cut(s)	527
BseMII	CTCAG	4 cut(s)	155, 261, 583, 1100
BseNI	ACTGG	5 cut(s)	230, 515, 819, 949, 971
BseXI	GCAGC	2 cut(s)	159, 1192
BseYI	CCCAGC	1 cut(s)	532
BsgI	GTGCAG	1 cut(s)	1199
BshFI	GGCC	6 cut(s)	5, 413, 429, 701, 728, 764
BsiHKAI	GWGCWC	1 cut(s)	618
BsiSI	CCGG	3 cut(s)	490, 791, 1184
BslFI	GGGAC	2 cut(s)	123, 1237
BslI	CCNNNNNNNGG	3 cut(s)	12, 446, 496
BsmAI	GTCTC	2 cut(s)	144, 1031
BsmFI	GGGAC	2 cut(s)	123, 1237
BsnI	GGCC	6 cut(s)	5, 413, 429, 701, 728, 764
Bsp1286I	GDGCHC	1 cut(s)	618
Bsp13I	TCCGGA	1 cut(s)	790
Bsp143I	GATC	5 cut(s)	203, 539, 774, 898, 1365
Bsp1720I	GCTNAGC	1 cut(s)	1109
BspACI	CCGC	4 cut(s)	318, 397, 640, 982
BspANI	GGCC	6 cut(s)	5, 413, 429, 701, 728, 764
BspCNI	CTCAG	4 cut(s)	154, 262, 582, 1101
BspEI	TCCGGA	1 cut(s)	790
BspHI	TCATGA	1 cut(s)	1081
BspMI	ACCTGC	1 cut(s)	241
BspOI	GCTAGC	1 cut(s)	1103
BspPI	GGATC	1 cut(s)	893
BspTI	CTTAAG	1 cut(s)	935
BsrDI	GCAATG	1 cut(s)	527
BsrI	ACTGG	5 cut(s)	230, 515, 819, 949, 971
BssECI	CCNNGG	1 cut(s)	12
BssMI	GATC	5 cut(s)	203, 539, 774, 898, 1365
BssNAI	GTATAC	1 cut(s)	473
BssSI	CACGAG	1 cut(s)	1034
Bst1107I	GTATAC	1 cut(s)	473
Bst2BI	CACGAG	1 cut(s)	1034
Bst2UI	CCWGG	3 cut(s)	13, 869, 1071
Bst4CI	ACNGT	3 cut(s)	1042, 1150, 1212
Bst6I	CTCTTC	3 cut(s)	228, 459, 570
BstAFI	CTTAAG	1 cut(s)	935
BstAPI	GCANNNNNTGC	1 cut(s)	967
BstC8I	GCNNGC	2 cut(s)	72, 1101
BstDEI	CTNAG	5 cut(s)	141, 200, 270, 569, 1109
BstEII	GGTNACC	1 cut(s)	16
BstF5I	GGATG	1 cut(s)	366
BstH2I	RGCGCY	1 cut(s)	1229
BstHHI	GCGC	1 cut(s)	1228
BstKTI	GATC	5 cut(s)	206, 542, 777, 901, 1368
BstMAI	GTCTC	2 cut(s)	144, 1031
BstMBI	GATC	5 cut(s)	203, 539, 774, 898, 1365
BstMWI	GCNNNNNNNGC	4 cut(s)	324, 338, 967, 1105
BstNI	CCWGG	3 cut(s)	13, 869, 1071
BstNSI	RCATGY	1 cut(s)	469
BstPI	GGTNACC	1 cut(s)	16
BstSCI	CCNGG	4 cut(s)	11, 489, 867, 1069
BstSFI	CTRYAG	1 cut(s)	1369
BstV1I	GCAGC	2 cut(s)	159, 1192
BstZ17I	GTATAC	1 cut(s)	473
BsuI	GTATCC	1 cut(s)	1339
BsuRI	GGCC	6 cut(s)	5, 413, 429, 701, 728, 764
BtgZI	GCGATG	1 cut(s)	352
BtsCI	GGATG	1 cut(s)	366
BtsIMutI	CAGTG	2 cut(s)	267, 1208
BveI	ACCTGC	1 cut(s)	241
Cac8I	GCNNGC	2 cut(s)	72, 1101
CciI	TCATGA	1 cut(s)	1081
CfoI	GCGC	1 cut(s)	1228
Cfr13I	GGNCC	1 cut(s)	257
CviAII	CATG	6 cut(s)	230, 466, 595, 840, 896, 1082
DdeI	CTNAG	5 cut(s)	141, 200, 270, 569, 1109
DpnI	GATC	5 cut(s)	205, 541, 776, 900, 1367
DpnII	GATC	5 cut(s)	203, 539, 774, 898, 1365
DraI	TTTAAA	1 cut(s)	1008
DraIII	CACNNNGTG	1 cut(s)	967
EaeI	YGGCCR	3 cut(s)	3, 411, 699
Eam1104I	CTCTTC	3 cut(s)	228, 459, 570
EarI	CTCTTC	3 cut(s)	228, 459, 570
EciI	GGCGGA	1 cut(s)	333
Ecl136II	GAGCTC	1 cut(s)	616
Eco147I	AGGCCT	1 cut(s)	429
Eco24I	GRGCYC	1 cut(s)	618
Eco47I	GGWCC	1 cut(s)	257
Eco53kI	GAGCTC	1 cut(s)	616
Eco57I	CTGAAG	2 cut(s)	203, 936
Eco91I	GGTNACC	1 cut(s)	16
EcoICRI	GAGCTC	1 cut(s)	616
EcoO65I	GGTNACC	1 cut(s)	16
EcoRI	GAATTC	1 cut(s)	385
EcoRII	CCWGG	3 cut(s)	11, 867, 1069
EcoT22I	ATGCAT	2 cut(s)	596, 1017
EcoT38I	GRGCYC	1 cut(s)	618
FaeI	CATG	6 cut(s)	233, 469, 598, 843, 899, 1085
FaqI	GGGAC	2 cut(s)	123, 1237
FatI	CATG	6 cut(s)	229, 465, 594, 839, 895, 1081
FauI	CCCGC	1 cut(s)	975
FblI	GTMKAC	1 cut(s)	472
Fnu4HI	GCNGC	2 cut(s)	148, 1181
FokI	GGATG	1 cut(s)	373
FriOI	GRGCYC	1 cut(s)	618
Fsp4HI	GCNGC	2 cut(s)	148, 1181
FspBI	CTAG	3 cut(s)	672, 1100, 1298
GlaI	GCGC	1 cut(s)	1227
GluI	GCNGC	2 cut(s)	148, 1181
GsaI	CCCAGC	1 cut(s)	536
HaeII	RGCGCY	1 cut(s)	1229
HaeIII	GGCC	6 cut(s)	5, 413, 429, 701, 728, 764
HapII	CCGG	3 cut(s)	490, 791, 1184
HhaI	GCGC	1 cut(s)	1228
Hin1II	CATG	6 cut(s)	233, 469, 598, 843, 899, 1085
Hin6I	GCGC	1 cut(s)	1226
HinP1I	GCGC	1 cut(s)	1226
HindIII	AAGCTT	2 cut(s)	68, 879
HinfI	GANTC	2 cut(s)	334, 521
HpaII	CCGG	3 cut(s)	490, 791, 1184
HphI	GGTGA	4 cut(s)	10, 959, 1188, 1301
Hpy166II	GTNNAC	2 cut(s)	473, 874
Hpy188I	TCNGA	3 cut(s)	85, 222, 572
Hpy188III	TCNNGA	3 cut(s)	791, 902, 1082
Hpy8I	GTNNAC	2 cut(s)	473, 874
HpyAV	CCTTC	3 cut(s)	688, 718, 972
HpyCH4III	ACNGT	3 cut(s)	1042, 1150, 1212
HpyCH4V	TGCA	7 cut(s)	62, 527, 594, 1015, 1021, 1056, 1180
HpyF10VI	GCNNNNNNNGC	4 cut(s)	324, 338, 967, 1105
HpyF3I	CTNAG	5 cut(s)	141, 200, 270, 569, 1109
Hsp92II	CATG	6 cut(s)	233, 469, 598, 843, 899, 1085
HspAI	GCGC	1 cut(s)	1226
Kpn2I	TCCGGA	1 cut(s)	790
Kzo9I	GATC	5 cut(s)	203, 539, 774, 898, 1365
LmnI	GCTCC	3 cut(s)	96, 161, 399
Lsp1109I	GCAGC	2 cut(s)	159, 1192
LweI	GCATC	5 cut(s)	375, 509, 581, 1024, 1324
MaeI	CTAG	3 cut(s)	672, 1100, 1298
MaeIII	GTNAC	3 cut(s)	16, 461, 1194
MalI	GATC	5 cut(s)	205, 541, 776, 900, 1367
MboI	GATC	5 cut(s)	203, 539, 774, 898, 1365
MboII	GAAGA	9 cut(s)	245, 446, 557, 703, 784, 929, 932, 1039, 1180
MfeI	CAATTG	1 cut(s)	648
MhlI	GDGCHC	1 cut(s)	618
MlsI	TGGCCA	3 cut(s)	5, 413, 701
MluNI	TGGCCA	3 cut(s)	5, 413, 701
MlyI	GAGTC	1 cut(s)	328
MmeI	TCCRAC	1 cut(s)	955
MnlI	CCTC	7 cut(s)	265, 368, 440, 989, 1265, 1294, 1336
Mox20I	TGGCCA	3 cut(s)	5, 413, 701
Mph1103I	ATGCAT	2 cut(s)	596, 1017
MroI	TCCGGA	1 cut(s)	790
MroXI	GAANNNNTTC	3 cut(s)	130, 216, 237
MscI	TGGCCA	3 cut(s)	5, 413, 701
MseI	TTAA	5 cut(s)	711, 911, 936, 1007, 1329
MslI	CAYNNNNRTG	1 cut(s)	1086
Msp20I	TGGCCA	3 cut(s)	5, 413, 701
MspA1I	CMGCKG	1 cut(s)	263
MspCI	CTTAAG	1 cut(s)	935
MspI	CCGG	3 cut(s)	490, 791, 1184
MspR9I	CCNGG	4 cut(s)	13, 491, 869, 1071
MunI	CAATTG	1 cut(s)	648
MvaI	CCWGG	3 cut(s)	13, 869, 1071
MwoI	GCNNNNNNNGC	4 cut(s)	324, 338, 967, 1105
NciI	CCSGG	1 cut(s)	491
NdeII	GATC	5 cut(s)	203, 539, 774, 898, 1365
NheI	GCTAGC	1 cut(s)	1099
NlaIII	CATG	6 cut(s)	233, 469, 598, 843, 899, 1085
NmeAIII	GCCGAG	2 cut(s)	273, 1084
NmuCI	GTSAC	3 cut(s)	16, 461, 1194
NsiI	ATGCAT	2 cut(s)	596, 1017
NspI	RCATGY	1 cut(s)	469
PagI	TCATGA	1 cut(s)	1081
PceI	AGGCCT	1 cut(s)	429
PciI	ACATGT	1 cut(s)	465
PcsI	WCGNNNNNNNCGW	1 cut(s)	751
PdmI	GAANNNNTTC	3 cut(s)	130, 216, 237
PfeI	GAWTC	1 cut(s)	521
PkrI	GCNGC	2 cut(s)	149, 1182
PleI	GAGTC	1 cut(s)	328
PpsI	GAGTC	1 cut(s)	328
PscI	ACATGT	1 cut(s)	465
Psp124BI	GAGCTC	1 cut(s)	618
Psp6I	CCWGG	3 cut(s)	11, 867, 1069
PspEI	GGTNACC	1 cut(s)	16
PspFI	CCCAGC	1 cut(s)	532
PspGI	CCWGG	3 cut(s)	11, 867, 1069
PspPI	GGNCC	1 cut(s)	257
PvuII	CAGCTG	1 cut(s)	263
RseI	CAYNNNNRTG	1 cut(s)	1086
SacI	GAGCTC	1 cut(s)	618
SaqAI	TTAA	5 cut(s)	711, 911, 936, 1007, 1329
SatI	GCNGC	2 cut(s)	148, 1181
Sau3AI	GATC	5 cut(s)	203, 539, 774, 898, 1365
Sau96I	GGNCC	1 cut(s)	257
SchI	GAGTC	1 cut(s)	328
ScrFI	CCNGG	4 cut(s)	13, 491, 869, 1071
SduI	GDGCHC	1 cut(s)	618
SfaNI	GCATC	5 cut(s)	375, 509, 581, 1024, 1324
SfcI	CTRYAG	1 cut(s)	1369
SinI	GGWCC	1 cut(s)	257
SmiMI	CAYNNNNRTG	1 cut(s)	1086
SmlI	CTYRAG	1 cut(s)	935
SmoI	CTYRAG	1 cut(s)	935
SpeI	ACTAGT	1 cut(s)	671
SseBI	AGGCCT	1 cut(s)	429
SsiI	CCGC	4 cut(s)	318, 397, 640, 982
SspMI	CTAG	3 cut(s)	672, 1100, 1298
SstI	GAGCTC	1 cut(s)	618
StuI	AGGCCT	1 cut(s)	429
StyD4I	CCNGG	4 cut(s)	11, 489, 867, 1069
TaaI	ACNGT	3 cut(s)	1042, 1150, 1212
TaqI	TCGA	3 cut(s)	538, 1230, 1257
TfiI	GAWTC	1 cut(s)	521
Tru1I	TTAA	5 cut(s)	711, 911, 936, 1007, 1329
Tru9I	TTAA	5 cut(s)	711, 911, 936, 1007, 1329
TscAI	CASTG	2 cut(s)	274, 1215
TseFI	GTSAC	3 cut(s)	16, 461, 1194
TseI	GCWGC	2 cut(s)	147, 1180
Tsp45I	GTSAC	3 cut(s)	16, 461, 1194
TspDTI	ATGAA	6 cut(s)	246, 496, 681, 753, 1070, 1098
TspRI	CASTG	2 cut(s)	274, 1215
Vha464I	CTTAAG	1 cut(s)	935
VpaK11BI	GGWCC	1 cut(s)	257
XapI	RAATTY	5 cut(s)	52, 385, 574, 863, 906
XceI	RCATGY	1 cut(s)	469
XmiI	GTMKAC	1 cut(s)	472
XmnI	GAANNNNTTC	3 cut(s)	130, 216, 237
XspI	CTAG	3 cut(s)	672, 1100, 1298
Zsp2I	ATGCAT	2 cut(s)	596, 1017

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.