AT4G13950

Ralf-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014259
Physical Location & Seq
Reverse (-)
8058072 .. 8058823
752 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G13950.1

Sequence Viewer

Length: 342 bp
ATGTTTAACTCTACAGCGTTGGTGATTTTCGCCATTCTTTTCCTCTTAATCTCCGCCGATGCGTTCCCGATTCCGTCTCCCAACGGAGAGATCGACGCAATGTTGATCCGAAACAGTATCATCGGAGAAGATGAAGATCTAATGCCAACTGAGATCAGCCGGAGAGTACTGATGGCGCAGAAACGGTACATCGGCTACGAAACGCTAAGGAGAGACATGGTTCCTTGTCAGAAACCAGGAGCTTCTTACTACGATTGTCGCTCTGGACAAGCTAATTCTTACAGCAGAGGCTGTGACACCATTACCAGATGCGCTAGAGACACAAACGACATCAACACATGA

Protein Analysis

113

Amino Acids

12.68

Weight (kDa)

5.01

Isoelectric Point (pI)

48.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 45 - 106 9.3e-27 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23805 AT4G13950
fragaria_vesca FvH4_4g13250
malus_domestica MD04G1017300.v1.1 MD13G1281800.v1.1
prunus_persica Prupe.1G165400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0413501
rosa_laevigata RLG00000008215
rosa_multiflora Rmu_sc0000883.1_g000006
rosa_roxburghii Rroxscaffold_5G00357400
rosa_rugosa Rorug04G0120700
rosa_samantha Rh4AG180400 Rh4BG179700 Rh4CG191500 Rh4DG175700
rosa_wichuraiana Rw4G015030 Rw4G015080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 54
AclWI GGATC 1 cut(s) 100
AfaI GTAC 2 cut(s) 168, 188
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 2 cut(s) 242, 272
AluI AGCT 2 cut(s) 242, 272
Alw26I GTCTC 3 cut(s) 81, 207, 312
AlwI GGATC 1 cut(s) 100
AlwNI CAGNNNCTG 1 cut(s) 291
AspLEI GCGC 2 cut(s) 178, 314
AsuHPI GGTGA 1 cut(s) 34
BccI CCATC 1 cut(s) 166
BciT130I CCWGG 1 cut(s) 237
BcoDI GTCTC 3 cut(s) 81, 207, 312
BfaI CTAG 1 cut(s) 315
BfmI CTRYAG 1 cut(s) 12
BglII AGATCT 1 cut(s) 136
BmcAI AGTACT 1 cut(s) 168
Bme1390I CCNGG 1 cut(s) 237
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 1 cut(s) 237
BmsI GCATC 2 cut(s) 49, 299
Bpu10I CCTNAGC 1 cut(s) 206
BsaBI GATNNNNATC 1 cut(s) 135
Bse3DI GCAATG 1 cut(s) 105
Bse8I GATNNNNATC 1 cut(s) 135
BseBI CCWGG 1 cut(s) 237
BseJI GATNNNNATC 1 cut(s) 135
BseMI GCAATG 1 cut(s) 105
BseMII CTCAG 1 cut(s) 141
BsiSI CCGG 1 cut(s) 160
BsmAI GTCTC 3 cut(s) 81, 207, 312
BsmBI CGTCTC 1 cut(s) 81
Bsp143I GATC 4 cut(s) 90, 105, 136, 153
BspACI CCGC 1 cut(s) 54
BspCNI CTCAG 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 222
BspPI GGATC 1 cut(s) 100
BsrDI GCAATG 1 cut(s) 105
BssMI GATC 4 cut(s) 90, 105, 136, 153
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 2 cut(s) 116, 186
BstDEI CTNAG 2 cut(s) 150, 206
BstHHI GCGC 2 cut(s) 178, 314
BstKTI GATC 4 cut(s) 93, 108, 139, 156
BstMAI GTCTC 3 cut(s) 81, 207, 312
BstMBI GATC 4 cut(s) 90, 105, 136, 153
BstNI CCWGG 1 cut(s) 237
BstSCI CCNGG 1 cut(s) 235
BstSFI CTRYAG 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
CaiI CAGNNNCTG 1 cut(s) 291
CfoI GCGC 2 cut(s) 178, 314
CseI GACGC 1 cut(s) 104
Csp6I GTAC 2 cut(s) 167, 187
CviAII CATG 2 cut(s) 217, 339
CviJI RGCY 5 cut(s) 159, 195, 242, 272, 291
CviKI_1 RGCY 5 cut(s) 159, 195, 242, 272, 291
CviQI GTAC 2 cut(s) 167, 187
DdeI CTNAG 2 cut(s) 150, 206
DpnI GATC 4 cut(s) 92, 107, 138, 155
DpnII GATC 4 cut(s) 90, 105, 136, 153
EciI GGCGGA 1 cut(s) 43
EcoRII CCWGG 1 cut(s) 235
Esp3I CGTCTC 1 cut(s) 81
FaeI CATG 2 cut(s) 220, 342
FaiI YATR 2 cut(s) 218, 340
FatI CATG 2 cut(s) 216, 338
FspBI CTAG 1 cut(s) 315
GlaI GCGC 2 cut(s) 177, 313
HapII CCGG 1 cut(s) 160
HgaI GACGC 1 cut(s) 104
HhaI GCGC 2 cut(s) 178, 314
Hin1II CATG 2 cut(s) 220, 342
Hin6I GCGC 2 cut(s) 176, 312
HinP1I GCGC 2 cut(s) 176, 312
HinfI GANTC 1 cut(s) 70
HpaII CCGG 1 cut(s) 160
HphI GGTGA 1 cut(s) 34
Hpy188I TCNGA 3 cut(s) 110, 125, 231
Hpy188III TCNNGA 2 cut(s) 67, 264
Hpy99I CGWCG 1 cut(s) 98
HpyCH4III ACNGT 2 cut(s) 116, 186
HpyF3I CTNAG 2 cut(s) 150, 206
Hsp92II CATG 2 cut(s) 220, 342
HspAI GCGC 2 cut(s) 176, 312
Kzo9I GATC 4 cut(s) 90, 105, 136, 153
LmnI GCTCC 1 cut(s) 239
LpnPI CCDG 5 cut(s) 173, 222, 249, 249, 319
LweI GCATC 2 cut(s) 49, 299
MaeI CTAG 1 cut(s) 315
MaeIII GTNAC 1 cut(s) 293
MalI GATC 4 cut(s) 92, 107, 138, 155
MboI GATC 4 cut(s) 90, 105, 136, 153
MboII GAAGA 2 cut(s) 140, 146
MflI RGATCY 1 cut(s) 136
MluCI AATT 1 cut(s) 274
MnlI CCTC 2 cut(s) 53, 281
MseI TTAA 2 cut(s) 6, 47
MspI CCGG 1 cut(s) 160
MspR9I CCNGG 1 cut(s) 237
MvaI CCWGG 1 cut(s) 237
NdeII GATC 4 cut(s) 90, 105, 136, 153
NlaIII CATG 2 cut(s) 220, 342
NlaIV GGNNCC 1 cut(s) 222
NmuCI GTSAC 1 cut(s) 293
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PfeI GAWTC 1 cut(s) 70
Psp6I CCWGG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 222
PstNI CAGNNNCTG 1 cut(s) 291
PsuI RGATCY 1 cut(s) 136
RsaI GTAC 2 cut(s) 168, 188
RsaNI GTAC 2 cut(s) 167, 187
SaqAI TTAA 2 cut(s) 6, 47
Sau3AI GATC 4 cut(s) 90, 105, 136, 153
ScaI AGTACT 1 cut(s) 168
ScrFI CCNGG 1 cut(s) 237
SetI ASST 2 cut(s) 244, 274
SfaNI GCATC 2 cut(s) 49, 299
SfcI CTRYAG 1 cut(s) 12
Sse9I AATT 1 cut(s) 274
SsiI CCGC 1 cut(s) 54
SspMI CTAG 1 cut(s) 315
StyD4I CCNGG 1 cut(s) 235
TaaI ACNGT 2 cut(s) 116, 186
TaqI TCGA 1 cut(s) 93
TasI AATT 1 cut(s) 274
TatI WGTACW 1 cut(s) 166
TfiI GAWTC 1 cut(s) 70
Tru1I TTAA 2 cut(s) 6, 47
Tru9I TTAA 2 cut(s) 6, 47
TseFI GTSAC 1 cut(s) 293
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 1 cut(s) 147
TspGWI ACGGA 2 cut(s) 63, 99
XspI CTAG 1 cut(s) 315
ZrmI AGTACT 1 cut(s) 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.