Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0014259

Physical Location & Seq

Genomic Coordinates Reverse (-)

2045450 .. 2045797

Gene Structure 348 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1017300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 348 bp

ATGCCCAAACCCACTATAATTTTCTACGTATCCCTCCTTGTAATCCACACCCACTTCCCAATCTGCAATGGGGTTTCACTCAAAGGGAGCGGAATCGAGGCTGCCATGGCGGGAAGAAGTGGCTGCAGCGAGAACCTTGGGGAGTGTTTGACAGAGCCGGAGATGGAGTCAGAGAGCAGCAGGAGGATTCTGGCAATGCAGAAGAAGTACATAAGCTACGGGACTCTCAAGAGGGACTCGGTTCCCTGTGGAACACCAGGGGCTTCCTACTACAACTGTCACGCTGTGGCGGCCAATCCGTACAACAGAGGGTGCGAGGTCATCACGGGATGTGCAAGAGGTAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005179 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005911 GO:0007154 GO:0007165 GO:0007267 GO:0008150 GO:0009506 GO:0009966 GO:0009987 GO:0010469 GO:0010646 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030054 GO:0030545 GO:0035556 GO:0048018 GO:0048046 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0065009 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RALF

Protein Analysis

116

Amino Acids

12.35

Weight (kDa)

8.18

Isoelectric Point (pI)

50.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RALF	PF05498	52 - 113	1.5e-27	Rapid ALkalinization Factor (RALF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0014259)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23805 AT4G13950
fragaria_vesca	FvH4_4g13250
malus_domestica	MD04G1017300.v1.1 MD13G1281800.v1.1
prunus_persica	Prupe.1G165400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0413501
rosa_laevigata	RLG00000008215
rosa_multiflora	Rmu_sc0000883.1_g000006
rosa_roxburghii	Rroxscaffold_5G00357400
rosa_rugosa	Rorug04G0120700
rosa_samantha	Rh4AG180400 Rh4BG179700 Rh4CG191500 Rh4DG175700
rosa_wichuraiana	Rw4G015030 Rw4G015080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	90
AciI	CCGC	3 cut(s)	90, 110, 290
AcoI	YGGCCR	1 cut(s)	291
AdeI	CACNNNGTG	1 cut(s)	286
AfaI	GTAC	2 cut(s)	209, 302
AjnI	CCWGG	1 cut(s)	256
AluBI	AGCT	1 cut(s)	216
AluI	AGCT	1 cut(s)	216
AoxI	GGCC	1 cut(s)	291
ApeKI	GCWGC	4 cut(s)	101, 123, 126, 177
BbvI	GCAGC	4 cut(s)	88, 110, 138, 189
BccI	CCATC	1 cut(s)	157
BciT130I	CCWGG	1 cut(s)	258
BciVI	GTATCC	1 cut(s)	40
BfmI	CTRYAG	1 cut(s)	124
BfuI	GTATCC	1 cut(s)	40
BisI	GCNGC	5 cut(s)	102, 124, 127, 178, 291
BlsI	GCNGC	5 cut(s)	103, 125, 128, 179, 292
Bme1390I	CCNGG	1 cut(s)	258
BmiI	GGNNCC	1 cut(s)	243
BmrFI	CCNGG	1 cut(s)	258
BpuEI	CTTGAG	1 cut(s)	212
BsaAI	YACGTR	1 cut(s)	28
BsaJI	CCNNGG	3 cut(s)	105, 136, 257
BsaXI	ACNNNNNCTCC	2 cut(s)	152, 182
Bse3DI	GCAATG	2 cut(s)	73, 201
BseBI	CCWGG	1 cut(s)	258
BseDI	CCNNGG	3 cut(s)	105, 136, 257
BseGI	GGATG	1 cut(s)	335
BseMI	GCAATG	2 cut(s)	73, 201
BseXI	GCAGC	4 cut(s)	88, 110, 138, 189
BshFI	GGCC	1 cut(s)	293
BsiSI	CCGG	1 cut(s)	158
BslFI	GGGAC	2 cut(s)	235, 248
BsmFI	GGGAC	2 cut(s)	235, 248
BsnI	GGCC	1 cut(s)	293
Bsp19I	CCATGG	1 cut(s)	105
BspACI	CCGC	3 cut(s)	90, 110, 290
BspANI	GGCC	1 cut(s)	293
BspLI	GGNNCC	1 cut(s)	243
BspMAI	CTGCAG	1 cut(s)	128
BsrBI	CCGCTC	1 cut(s)	90
BsrDI	GCAATG	2 cut(s)	73, 201
BssECI	CCNNGG	3 cut(s)	105, 136, 257
BssT1I	CCWWGG	2 cut(s)	105, 136
Bst2UI	CCWGG	1 cut(s)	258
Bst4CI	ACNGT	1 cut(s)	278
BstBAI	YACGTR	1 cut(s)	28
BstDSI	CCRYGG	1 cut(s)	105
BstF5I	GGATG	1 cut(s)	335
BstMWI	GCNNNNNNNGC	2 cut(s)	107, 290
BstNI	CCWGG	1 cut(s)	258
BstSCI	CCNGG	1 cut(s)	256
BstSFI	CTRYAG	1 cut(s)	124
BstSNI	TACGTA	1 cut(s)	28
BstV1I	GCAGC	4 cut(s)	88, 110, 138, 189
BsuI	GTATCC	1 cut(s)	40
BsuRI	GGCC	1 cut(s)	293
BtgI	CCRYGG	1 cut(s)	105
BtsCI	GGATG	1 cut(s)	335
Csp6I	GTAC	2 cut(s)	208, 301
CviAII	CATG	1 cut(s)	106
CviJI	RGCY	6 cut(s)	101, 123, 157, 216, 263, 293
CviKI_1	RGCY	6 cut(s)	101, 123, 157, 216, 263, 293
CviQI	GTAC	2 cut(s)	208, 301
DraIII	CACNNNGTG	1 cut(s)	286
EaeI	YGGCCR	1 cut(s)	291
Eco105I	TACGTA	1 cut(s)	28
Eco130I	CCWWGG	2 cut(s)	105, 136
EcoRII	CCWGG	1 cut(s)	256
EcoT14I	CCWWGG	2 cut(s)	105, 136
ErhI	CCWWGG	2 cut(s)	105, 136
FaeI	CATG	1 cut(s)	109
FaiI	YATR	3 cut(s)	17, 107, 212
FaqI	GGGAC	2 cut(s)	235, 248
FatI	CATG	1 cut(s)	105
FauI	CCCGC	1 cut(s)	103
Fnu4HI	GCNGC	5 cut(s)	102, 124, 127, 178, 291
FokI	GGATG	1 cut(s)	342
Fsp4HI	GCNGC	5 cut(s)	102, 124, 127, 178, 291
GluI	GCNGC	5 cut(s)	102, 124, 127, 178, 291
HaeIII	GGCC	1 cut(s)	293
HapII	CCGG	1 cut(s)	158
Hin1II	CATG	1 cut(s)	109
HinfI	GANTC	5 cut(s)	93, 167, 187, 223, 236
HpaII	CCGG	1 cut(s)	158
Hpy188I	TCNGA	1 cut(s)	172
Hpy188III	TCNNGA	1 cut(s)	229
HpyCH4III	ACNGT	1 cut(s)	278
HpyCH4IV	ACGT	1 cut(s)	27
HpyCH4V	TGCA	4 cut(s)	66, 126, 199, 335
HpyF10VI	GCNNNNNNNGC	2 cut(s)	107, 290
HpySE526I	ACGT	1 cut(s)	27
Hsp92II	CATG	1 cut(s)	109
LmnI	GCTCC	1 cut(s)	87
LpnPI	CCDG	6 cut(s)	166, 171, 176, 243, 259, 270
Lsp1109I	GCAGC	4 cut(s)	88, 110, 138, 189
MaeII	ACGT	1 cut(s)	27
MaeIII	GTNAC	1 cut(s)	278
MbiI	CCGCTC	1 cut(s)	90
MboII	GAAGA	2 cut(s)	126, 214
MluCI	AATT	2 cut(s)	18, 343
MlyI	GAGTC	3 cut(s)	176, 217, 230
MnlI	CCTC	7 cut(s)	44, 91, 177, 225, 302, 310, 332
MspI	CCGG	1 cut(s)	158
MspR9I	CCNGG	1 cut(s)	258
MvaI	CCWGG	1 cut(s)	258
MwoI	GCNNNNNNNGC	2 cut(s)	107, 290
NcoI	CCATGG	1 cut(s)	105
NlaIII	CATG	1 cut(s)	109
NlaIV	GGNNCC	1 cut(s)	243
NmuCI	GTSAC	1 cut(s)	278
PfeI	GAWTC	2 cut(s)	93, 187
PkrI	GCNGC	5 cut(s)	103, 125, 128, 179, 292
PleI	GAGTC	3 cut(s)	175, 217, 230
PpsI	GAGTC	3 cut(s)	175, 217, 230
Ppu21I	YACGTR	1 cut(s)	28
Psp6I	CCWGG	1 cut(s)	256
PspGI	CCWGG	1 cut(s)	256
PspN4I	GGNNCC	1 cut(s)	243
PstI	CTGCAG	1 cut(s)	128
RsaI	GTAC	2 cut(s)	209, 302
RsaNI	GTAC	2 cut(s)	208, 301
SatI	GCNGC	5 cut(s)	102, 124, 127, 178, 291
SchI	GAGTC	3 cut(s)	176, 217, 230
ScrFI	CCNGG	1 cut(s)	258
SetI	ASST	5 cut(s)	30, 138, 218, 321, 343
SfcI	CTRYAG	1 cut(s)	124
SmlI	CTYRAG	1 cut(s)	227
SmoI	CTYRAG	1 cut(s)	227
SnaBI	TACGTA	1 cut(s)	28
Sse9I	AATT	2 cut(s)	18, 343
SsiI	CCGC	3 cut(s)	90, 110, 290
StyD4I	CCNGG	1 cut(s)	256
StyI	CCWWGG	2 cut(s)	105, 136
TaaI	ACNGT	1 cut(s)	278
TaiI	ACGT	1 cut(s)	30
TaqI	TCGA	1 cut(s)	96
TasI	AATT	2 cut(s)	18, 343
TatI	WGTACW	1 cut(s)	207
TauI	GCSGC	1 cut(s)	293
TfiI	GAWTC	2 cut(s)	93, 187
TseFI	GTSAC	1 cut(s)	278
TseI	GCWGC	4 cut(s)	101, 123, 126, 177
Tsp45I	GTSAC	1 cut(s)	278
TspGWI	ACGGA	1 cut(s)	288

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.