FvH4_4g13250

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014259
Physical Location & Seq
Forward (+)
16854377 .. 16855101
725 bp
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UTR
Exon/CDS
Intron
FvH4_4g13250.t1

Sequence Viewer

Length: 459 bp
ATGGCTGATGTCCCAACCCCCTTTATGAGCCATCCAACCACCCAGAATCTCACTCAACTAGTCCCAACAACAAAACCACAAACAAGAACCCCCAAAAGAGAGAAAATGAAAATGTCCAAACTCAGAGACAATCCCATGTTAATCTTCTACCTGGGTCTCCTCTTAATTCTCACCCATTTCTCAATCTGCAATGGGTCTTCAGTTCTGGACCTCAATGCACTCAAAGGAAGTGAAACTGATGTCAAGGTCGGGGACTGCATAACAGAGCCGGAGATGGACTCAGAGACAAATAGAAGAGTGCTAGCAATGCAGAAGAAGTACATAAGCTACGAGACACTGAAGAAGGACTTGGTTCCTTGTGCAAGGCCTGGGGCTTCCTACTACAATTGCCATGCTGTGGCGGCCAATCCTTATAATAGAGGCTGTGAGGTCATTACACGATGTAGAGGTACCCCATGA

Protein Analysis

153

Amino Acids

17.04

Weight (kDa)

9.07

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 89 - 150 3.9e-28 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23805 AT4G13950
fragaria_vesca FvH4_4g13250
malus_domestica MD04G1017300.v1.1 MD13G1281800.v1.1
prunus_persica Prupe.1G165400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0413501
rosa_laevigata RLG00000008215
rosa_multiflora Rmu_sc0000883.1_g000006
rosa_roxburghii Rroxscaffold_5G00357400
rosa_rugosa Rorug04G0120700
rosa_samantha Rh4AG180400 Rh4BG179700 Rh4CG191500 Rh4DG175700
rosa_wichuraiana Rw4G015030 Rw4G015080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 414
Acc65I GGTACC 1 cut(s) 449
AccB1I GGYRCC 1 cut(s) 449
AccB7I CCANNNNNTGG 1 cut(s) 397
AciI CCGC 1 cut(s) 401
AcoI YGGCCR 1 cut(s) 402
AcuI CTGAAG 2 cut(s) 183, 359
AfaI GTAC 2 cut(s) 320, 451
AfiI CCNNNNNNNGG 1 cut(s) 397
AhlI ACTAGT 1 cut(s) 58
AjnI CCWGG 2 cut(s) 150, 367
AjuI GAANNNNNNNTTGG 2 cut(s) 332, 364
AluBI AGCT 1 cut(s) 327
AluI AGCT 1 cut(s) 327
Alw26I GTCTC 4 cut(s) 120, 161, 278, 326
AoxI GGCC 2 cut(s) 365, 402
Asp718I GGTACC 1 cut(s) 449
AspS9I GGNCC 1 cut(s) 208
AsuHPI GGTGA 1 cut(s) 163
AsuNHI GCTAGC 1 cut(s) 301
AvaII GGWCC 1 cut(s) 208
BanI GGYRCC 1 cut(s) 449
BbsI GAAGAC 1 cut(s) 189
BccI CCATC 2 cut(s) 39, 268
BciT130I CCWGG 2 cut(s) 152, 369
BcoDI GTCTC 4 cut(s) 120, 161, 278, 326
BcuI ACTAGT 1 cut(s) 58
BfaI CTAG 2 cut(s) 59, 302
BisI GCNGC 1 cut(s) 402
BlsI GCNGC 1 cut(s) 403
Bme1390I CCNGG 2 cut(s) 152, 369
Bme18I GGWCC 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 2 cut(s) 354, 451
BmrFI CCNGG 2 cut(s) 152, 369
BmtI GCTAGC 1 cut(s) 305
BpiI GAAGAC 1 cut(s) 189
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 2 cut(s) 151, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 397
Bse3DI GCAATG 2 cut(s) 196, 312
BseBI CCWGG 2 cut(s) 152, 369
BseDI CCNNGG 2 cut(s) 151, 368
BseGI GGATG 1 cut(s) 31
BseLI CCNNNNNNNGG 1 cut(s) 397
BseMI GCAATG 2 cut(s) 196, 312
BseMII CTCAG 2 cut(s) 136, 294
BseRI GAGGAG 1 cut(s) 149
BshFI GGCC 2 cut(s) 367, 404
BshNI GGYRCC 1 cut(s) 449
BsiSI CCGG 1 cut(s) 269
BslFI GGGAC 2 cut(s) 47, 266
BslI CCNNNNNNNGG 1 cut(s) 397
BsmAI GTCTC 4 cut(s) 120, 161, 278, 326
BsmFI GGGAC 2 cut(s) 47, 266
BsnI GGCC 2 cut(s) 367, 404
Bso31I GGTCTC 1 cut(s) 161
BspACI CCGC 1 cut(s) 401
BspANI GGCC 2 cut(s) 367, 404
BspCNI CTCAG 2 cut(s) 135, 293
BspLI GGNNCC 2 cut(s) 354, 451
BspOI GCTAGC 1 cut(s) 305
BspT107I GGYRCC 1 cut(s) 449
BspTNI GGTCTC 1 cut(s) 161
BsrDI GCAATG 2 cut(s) 196, 312
BssECI CCNNGG 2 cut(s) 151, 368
Bst2UI CCWGG 2 cut(s) 152, 369
Bst6I CTCTTC 1 cut(s) 289
BstC8I GCNNGC 1 cut(s) 303
BstDEI CTNAG 2 cut(s) 122, 280
BstF5I GGATG 1 cut(s) 31
BstMAI GTCTC 4 cut(s) 120, 161, 278, 326
BstMWI GCNNNNNNNGC 2 cut(s) 307, 401
BstNI CCWGG 2 cut(s) 152, 369
BstSCI CCNGG 2 cut(s) 150, 367
BstV2I GAAGAC 1 cut(s) 189
BsuRI GGCC 2 cut(s) 367, 404
BtsCI GGATG 1 cut(s) 31
BtsIMutI CAGTG 1 cut(s) 335
Cac8I GCNNGC 1 cut(s) 303
Cfr13I GGNCC 1 cut(s) 208
Csp6I GTAC 2 cut(s) 319, 450
CviAII CATG 3 cut(s) 136, 392, 456
CviJI RGCY 8 cut(s) 5, 30, 268, 327, 367, 374, 404, 423
CviKI_1 RGCY 8 cut(s) 5, 30, 268, 327, 367, 374, 404, 423
CviQI GTAC 2 cut(s) 319, 450
DdeI CTNAG 2 cut(s) 122, 280
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 289
Eco147I AGGCCT 1 cut(s) 367
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 208
Eco57I CTGAAG 2 cut(s) 183, 359
EcoRII CCWGG 2 cut(s) 150, 367
FaeI CATG 3 cut(s) 139, 395, 459
FaiI YATR 7 cut(s) 26, 137, 260, 323, 393, 414, 457
FalI AAGNNNNNCTT 2 cut(s) 332, 364
FaqI GGGAC 2 cut(s) 47, 266
FatI CATG 3 cut(s) 135, 391, 455
Fnu4HI GCNGC 1 cut(s) 402
FokI GGATG 1 cut(s) 18
Fsp4HI GCNGC 1 cut(s) 402
FspBI CTAG 2 cut(s) 59, 302
GluI GCNGC 1 cut(s) 402
HaeIII GGCC 2 cut(s) 367, 404
HapII CCGG 1 cut(s) 269
Hin1II CATG 3 cut(s) 139, 395, 459
HinfI GANTC 2 cut(s) 46, 278
HpaII CCGG 1 cut(s) 269
HphI GGTGA 1 cut(s) 163
Hpy188I TCNGA 2 cut(s) 125, 283
Hpy188III TCNNGA 1 cut(s) 206
HpyAV CCTTC 1 cut(s) 337
HpyCH4V TGCA 5 cut(s) 189, 218, 258, 310, 362
HpyF10VI GCNNNNNNNGC 2 cut(s) 307, 401
HpyF3I CTNAG 2 cut(s) 122, 280
Hsp92II CATG 3 cut(s) 139, 395, 459
KpnI GGTACC 1 cut(s) 453
LpnPI CCDG 7 cut(s) 56, 137, 164, 191, 282, 354, 381
MaeI CTAG 2 cut(s) 59, 302
MboII GAAGA 5 cut(s) 136, 189, 306, 325, 352
MfeI CAATTG 1 cut(s) 385
MluCI AATT 2 cut(s) 165, 385
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 1 cut(s) 59
MnlI CCTC 5 cut(s) 170, 221, 413, 421, 440
MseI TTAA 2 cut(s) 140, 164
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 2 cut(s) 152, 369
MunI CAATTG 1 cut(s) 385
MvaI CCWGG 2 cut(s) 152, 369
MwoI GCNNNNNNNGC 2 cut(s) 307, 401
NheI GCTAGC 1 cut(s) 301
NlaIII CATG 3 cut(s) 139, 395, 459
NlaIV GGNNCC 2 cut(s) 354, 451
PceI AGGCCT 1 cut(s) 367
PfeI GAWTC 1 cut(s) 46
PflMI CCANNNNNTGG 1 cut(s) 397
PkrI GCNGC 1 cut(s) 403
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PsiI TTATAA 1 cut(s) 414
Psp6I CCWGG 2 cut(s) 150, 367
PspGI CCWGG 2 cut(s) 150, 367
PspN4I GGNNCC 2 cut(s) 354, 451
PspPI GGNCC 1 cut(s) 208
RsaI GTAC 2 cut(s) 320, 451
RsaNI GTAC 2 cut(s) 319, 450
SaqAI TTAA 2 cut(s) 140, 164
SatI GCNGC 1 cut(s) 402
Sau96I GGNCC 1 cut(s) 208
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 2 cut(s) 152, 369
SetI ASST 6 cut(s) 153, 213, 249, 329, 432, 451
SinI GGWCC 1 cut(s) 208
SpeI ACTAGT 1 cut(s) 58
Sse9I AATT 2 cut(s) 165, 385
SseBI AGGCCT 1 cut(s) 367
SsiI CCGC 1 cut(s) 401
SspMI CTAG 2 cut(s) 59, 302
StuI AGGCCT 1 cut(s) 367
StyD4I CCNGG 2 cut(s) 150, 367
TasI AATT 2 cut(s) 165, 385
TatI WGTACW 1 cut(s) 318
TauI GCSGC 1 cut(s) 404
TfiI GAWTC 1 cut(s) 46
Tru1I TTAA 2 cut(s) 140, 164
Tru9I TTAA 2 cut(s) 140, 164
TscAI CASTG 1 cut(s) 342
TspDTI ATGAA 1 cut(s) 122
TspRI CASTG 1 cut(s) 342
Van91I CCANNNNNTGG 1 cut(s) 397
VpaK11BI GGWCC 1 cut(s) 208
XspI CTAG 2 cut(s) 59, 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.