Rh4BG179700

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014259
Physical Location & Seq
Forward (+)
31762735 .. 31763091
357 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG179700.1

Sequence Viewer

Length: 357 bp
ATGTCCAAACTCAGAGACAATCCCATCTTAATCTTGTATCTTGGCCTCCTCCTAATCCTTACCCATTTCTCAATCTGCAATGGGGATTCAGTTCTTGACCTCAGTGCACTCAAAGGAAGTGAAGTTGATGTCAAGGTTGGGGACTTCATAACAGAGCCAGAGATGGACTCAGAGATAAGTAGAAGAGTGCTAGCGATGCAGAAGAAGTACATAAGCTATGAGACACTGAGGAGGGACTTGGTTCCTTGTGCAAGGCCAGGTGCTTCATACTACAATTGCCATGCTGTGGCCAACCCTTATAATAGAGGCTGTGAGGTTATCACAGGATGTAGAGGTACACATGACATCAAAACTTGA

Protein Analysis

118

Amino Acids

13.18

Weight (kDa)

6.81

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 52 - 112 4.1e-27 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23805 AT4G13950
fragaria_vesca FvH4_4g13250
malus_domestica MD04G1017300.v1.1 MD13G1281800.v1.1
prunus_persica Prupe.1G165400_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0413501
rosa_laevigata RLG00000008215
rosa_multiflora Rmu_sc0000883.1_g000006
rosa_roxburghii Rroxscaffold_5G00357400
rosa_rugosa Rorug04G0120700
rosa_samantha Rh4AG180400 Rh4BG179700 Rh4CG191500 Rh4DG175700
rosa_wichuraiana Rw4G015030 Rw4G015080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 300
AccB7I CCANNNNNTGG 1 cut(s) 286
AcoI YGGCCR 1 cut(s) 288
AfaI GTAC 2 cut(s) 209, 337
AfiI CCNNNNNNNGG 1 cut(s) 286
AjnI CCWGG 1 cut(s) 256
AluBI AGCT 1 cut(s) 216
AluI AGCT 1 cut(s) 216
Alw21I GWGCWC 1 cut(s) 109
Alw26I GTCTC 2 cut(s) 9, 215
Alw44I GTGCAC 1 cut(s) 105
AoxI GGCC 3 cut(s) 43, 254, 288
ApaLI GTGCAC 1 cut(s) 105
AsuNHI GCTAGC 1 cut(s) 190
BaeGI GKGCMC 1 cut(s) 109
BalI TGGCCA 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 109
BccI CCATC 2 cut(s) 32, 157
BciT130I CCWGG 1 cut(s) 258
BcoDI GTCTC 2 cut(s) 9, 215
BfaI CTAG 1 cut(s) 191
Bme1390I CCNGG 1 cut(s) 258
BmiI GGNNCC 1 cut(s) 243
BmrFI CCNGG 1 cut(s) 258
BmsI GCATC 1 cut(s) 186
BmtI GCTAGC 1 cut(s) 194
BplI GAGNNNNNCTC 2 cut(s) 152, 184
Bsc4I CCNNNNNNNGG 1 cut(s) 286
Bse3DI GCAATG 1 cut(s) 85
BseBI CCWGG 1 cut(s) 258
BseGI GGATG 1 cut(s) 332
BseLI CCNNNNNNNGG 1 cut(s) 286
BseMI GCAATG 1 cut(s) 85
BseMII CTCAG 4 cut(s) 25, 115, 183, 218
BseRI GAGGAG 2 cut(s) 38, 244
BseSI GKGCMC 1 cut(s) 109
BshFI GGCC 3 cut(s) 45, 256, 290
BsiHKAI GWGCWC 1 cut(s) 109
BslFI GGGAC 2 cut(s) 155, 248
BslI CCNNNNNNNGG 1 cut(s) 286
BsmAI GTCTC 2 cut(s) 9, 215
BsmFI GGGAC 2 cut(s) 155, 248
BsnI GGCC 3 cut(s) 45, 256, 290
Bsp1286I GDGCHC 1 cut(s) 109
BspANI GGCC 3 cut(s) 45, 256, 290
BspCNI CTCAG 4 cut(s) 24, 114, 182, 219
BspLI GGNNCC 1 cut(s) 243
BspOI GCTAGC 1 cut(s) 194
BsrDI GCAATG 1 cut(s) 85
Bst2UI CCWGG 1 cut(s) 258
Bst6I CTCTTC 1 cut(s) 178
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 4 cut(s) 11, 101, 169, 227
BstF5I GGATG 1 cut(s) 332
BstMAI GTCTC 2 cut(s) 9, 215
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstNI CCWGG 1 cut(s) 258
BstSCI CCNGG 1 cut(s) 256
BstSLI GKGCMC 1 cut(s) 109
BsuRI GGCC 3 cut(s) 45, 256, 290
BtgZI GCGATG 1 cut(s) 209
BtsCI GGATG 1 cut(s) 332
BtsIMutI CAGTG 2 cut(s) 109, 224
Cac8I GCNNGC 1 cut(s) 192
Csp6I GTAC 2 cut(s) 208, 336
CviAII CATG 2 cut(s) 281, 341
CviJI RGCY 6 cut(s) 45, 157, 216, 256, 290, 309
CviKI_1 RGCY 6 cut(s) 45, 157, 216, 256, 290, 309
CviQI GTAC 2 cut(s) 208, 336
DdeI CTNAG 4 cut(s) 11, 101, 169, 227
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
EcoRII CCWGG 1 cut(s) 256
FaeI CATG 2 cut(s) 284, 344
FaiI YATR 7 cut(s) 149, 212, 219, 268, 282, 300, 342
FaqI GGGAC 2 cut(s) 155, 248
FatI CATG 2 cut(s) 280, 340
FokI GGATG 1 cut(s) 339
FspBI CTAG 1 cut(s) 191
HaeIII GGCC 3 cut(s) 45, 256, 290
Hin1II CATG 2 cut(s) 284, 344
HinfI GANTC 2 cut(s) 86, 167
Hpy166II GTNNAC 2 cut(s) 107, 338
Hpy188I TCNGA 2 cut(s) 14, 172
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 2 cut(s) 107, 338
HpyCH4V TGCA 4 cut(s) 78, 107, 199, 251
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
HpyF3I CTNAG 4 cut(s) 11, 101, 169, 227
Hsp92II CATG 2 cut(s) 284, 344
LpnPI CCDG 4 cut(s) 171, 243, 270, 309
LweI GCATC 1 cut(s) 186
MaeI CTAG 1 cut(s) 191
MboII GAAGA 2 cut(s) 195, 214
MfeI CAATTG 1 cut(s) 274
MhlI GDGCHC 1 cut(s) 109
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 1 cut(s) 274
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 1 cut(s) 161
MnlI CCTC 8 cut(s) 56, 59, 110, 222, 225, 299, 307, 326
Mox20I TGGCCA 1 cut(s) 290
MscI TGGCCA 1 cut(s) 290
MseI TTAA 1 cut(s) 29
Msp20I TGGCCA 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 258
MunI CAATTG 1 cut(s) 274
MvaI CCWGG 1 cut(s) 258
MwoI GCNNNNNNNGC 1 cut(s) 196
NheI GCTAGC 1 cut(s) 190
NlaIII CATG 2 cut(s) 284, 344
NlaIV GGNNCC 1 cut(s) 243
PfeI GAWTC 1 cut(s) 86
PflMI CCANNNNNTGG 1 cut(s) 286
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
PsiI TTATAA 1 cut(s) 300
Psp6I CCWGG 1 cut(s) 256
PspGI CCWGG 1 cut(s) 256
PspN4I GGNNCC 1 cut(s) 243
RsaI GTAC 2 cut(s) 209, 337
RsaNI GTAC 2 cut(s) 208, 336
SaqAI TTAA 1 cut(s) 29
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 1 cut(s) 258
SduI GDGCHC 1 cut(s) 109
SetI ASST 6 cut(s) 102, 138, 218, 262, 318, 337
SfaNI GCATC 1 cut(s) 186
Sse9I AATT 1 cut(s) 274
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 1 cut(s) 256
TasI AATT 1 cut(s) 274
TatI WGTACW 1 cut(s) 207
TfiI GAWTC 1 cut(s) 86
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TscAI CASTG 2 cut(s) 109, 231
TspDTI ATGAA 2 cut(s) 136, 255
TspRI CASTG 2 cut(s) 109, 231
Van91I CCANNNNNTGG 1 cut(s) 286
VneI GTGCAC 1 cut(s) 105
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.