Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0014259

Physical Location & Seq

Genomic Coordinates Forward (+)

34746627 .. 34747282

Gene Structure 656 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G015080.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 441 bp

ATGAGCCATCCAACCACCCAGAATCCCAGTCTCCCAGTCCCAAAAACAAAAACACAAAGAAGCCCCAAAACAGAGAACATCAAAATGTTCAAACTCAGGGACAATCCCATCTTAATCTTGTATCTTGGCCTCCTCTTGATCCTTACCCATTTCTCAATCTGCAATGGGGTGTCAGTTCTTGACCTTAGTGCACTCAAAGGAAGTGAAATTGATGTCAAGGTTGGGGACTTCATAACAGAGCCAGAGATGGACTCAGAGATAAGTAGAAGAGTGCTAGCAATGCAGAAGAAGTACATAAGCTATGAGACACTGAGGAGGGACTTGGTTCCTTGTGCAAGGCCAGGGGCTTCATACTACAATTGCCATGCTGTGGCCAACCCTTATAATAGAGGCTGTGAGGTTATCACAGGATGTAGAGGTACACATGACATCAAAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005102 GO:0005179 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005911 GO:0007154 GO:0007165 GO:0007267 GO:0008150 GO:0009506 GO:0009966 GO:0009987 GO:0010469 GO:0010646 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030054 GO:0030545 GO:0035556 GO:0048018 GO:0048046 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0065007 GO:0065009 GO:0098772

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RALF

Protein Analysis

146

Amino Acids

16.37

Weight (kDa)

9.01

Isoelectric Point (pI)

48.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RALF	PF05498	80 - 140	6.8e-27	Rapid ALkalinization Factor (RALF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014259)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G23805 AT4G13950
fragaria_vesca	FvH4_4g13250
malus_domestica	MD04G1017300.v1.1 MD13G1281800.v1.1
prunus_persica	Prupe.1G165400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0413501
rosa_laevigata	RLG00000008215
rosa_multiflora	Rmu_sc0000883.1_g000006
rosa_roxburghii	Rroxscaffold_5G00357400
rosa_rugosa	Rorug04G0120700
rosa_samantha	Rh4AG180400 Rh4BG179700 Rh4CG191500 Rh4DG175700
rosa_wichuraiana	Rw4G015030 Rw4G015080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	384
AccB7I	CCANNNNNTGG	1 cut(s)	370
AclWI	GGATC	1 cut(s)	133
AcoI	YGGCCR	1 cut(s)	372
AfaI	GTAC	2 cut(s)	293, 421
AfiI	CCNNNNNNNGG	1 cut(s)	370
AgsI	TTSAA	1 cut(s)	91
AjnI	CCWGG	1 cut(s)	340
AluBI	AGCT	1 cut(s)	300
AluI	AGCT	1 cut(s)	300
Alw21I	GWGCWC	1 cut(s)	193
Alw26I	GTCTC	2 cut(s)	35, 299
Alw44I	GTGCAC	1 cut(s)	189
AlwI	GGATC	1 cut(s)	133
AoxI	GGCC	3 cut(s)	127, 338, 372
ApaLI	GTGCAC	1 cut(s)	189
AsuNHI	GCTAGC	1 cut(s)	274
BaeGI	GKGCMC	1 cut(s)	193
BalI	TGGCCA	1 cut(s)	374
Bbv12I	GWGCWC	1 cut(s)	193
BccI	CCATC	3 cut(s)	15, 116, 241
BciT130I	CCWGG	1 cut(s)	342
BcoDI	GTCTC	2 cut(s)	35, 299
BfaI	CTAG	1 cut(s)	275
Bme1390I	CCNGG	1 cut(s)	342
BmiI	GGNNCC	1 cut(s)	327
BmrFI	CCNGG	1 cut(s)	342
BmrI	ACTGGG	2 cut(s)	21, 29
BmtI	GCTAGC	1 cut(s)	278
BmuI	ACTGGG	2 cut(s)	21, 29
BplI	GAGNNNNNCTC	2 cut(s)	236, 268
BsaJI	CCNNGG	1 cut(s)	341
Bsc4I	CCNNNNNNNGG	1 cut(s)	370
Bse1I	ACTGG	2 cut(s)	27, 35
Bse3DI	GCAATG	2 cut(s)	169, 285
BseBI	CCWGG	1 cut(s)	342
BseDI	CCNNGG	1 cut(s)	341
BseGI	GGATG	2 cut(s)	7, 416
BseLI	CCNNNNNNNGG	1 cut(s)	370
BseMI	GCAATG	2 cut(s)	169, 285
BseMII	CTCAG	3 cut(s)	109, 267, 302
BseNI	ACTGG	2 cut(s)	27, 35
BseRI	GAGGAG	2 cut(s)	122, 328
BseSI	GKGCMC	1 cut(s)	193
BshFI	GGCC	3 cut(s)	129, 340, 374
BsiHKAI	GWGCWC	1 cut(s)	193
BslFI	GGGAC	4 cut(s)	23, 113, 239, 332
BslI	CCNNNNNNNGG	1 cut(s)	370
BsmAI	GTCTC	2 cut(s)	35, 299
BsmFI	GGGAC	4 cut(s)	23, 113, 239, 332
BsnI	GGCC	3 cut(s)	129, 340, 374
Bsp1286I	GDGCHC	1 cut(s)	193
Bsp143I	GATC	1 cut(s)	138
BspANI	GGCC	3 cut(s)	129, 340, 374
BspCNI	CTCAG	3 cut(s)	108, 266, 303
BspLI	GGNNCC	1 cut(s)	327
BspOI	GCTAGC	1 cut(s)	278
BspPI	GGATC	1 cut(s)	133
BsrDI	GCAATG	2 cut(s)	169, 285
BsrI	ACTGG	2 cut(s)	27, 35
BssECI	CCNNGG	1 cut(s)	341
BssMI	GATC	1 cut(s)	138
Bst2UI	CCWGG	1 cut(s)	342
Bst6I	CTCTTC	1 cut(s)	262
BstC8I	GCNNGC	1 cut(s)	276
BstDEI	CTNAG	4 cut(s)	95, 185, 253, 311
BstF5I	GGATG	2 cut(s)	7, 416
BstKTI	GATC	1 cut(s)	141
BstMAI	GTCTC	2 cut(s)	35, 299
BstMBI	GATC	1 cut(s)	138
BstMWI	GCNNNNNNNGC	1 cut(s)	280
BstNI	CCWGG	1 cut(s)	342
BstSCI	CCNGG	1 cut(s)	340
BstSLI	GKGCMC	1 cut(s)	193
BsuRI	GGCC	3 cut(s)	129, 340, 374
BtsCI	GGATG	2 cut(s)	7, 416
BtsIMutI	CAGTG	1 cut(s)	308
Cac8I	GCNNGC	1 cut(s)	276
Csp6I	GTAC	2 cut(s)	292, 420
CviAII	CATG	2 cut(s)	365, 425
CviJI	RGCY	9 cut(s)	6, 63, 129, 241, 300, 340, 347, 374, 393
CviKI_1	RGCY	9 cut(s)	6, 63, 129, 241, 300, 340, 347, 374, 393
CviQI	GTAC	2 cut(s)	292, 420
DdeI	CTNAG	4 cut(s)	95, 185, 253, 311
DpnI	GATC	1 cut(s)	140
DpnII	GATC	1 cut(s)	138
EaeI	YGGCCR	1 cut(s)	372
Eam1104I	CTCTTC	1 cut(s)	262
EarI	CTCTTC	1 cut(s)	262
EcoRII	CCWGG	1 cut(s)	340
FaeI	CATG	2 cut(s)	368, 428
FaiI	YATR	7 cut(s)	233, 296, 303, 352, 366, 384, 426
FaqI	GGGAC	4 cut(s)	23, 113, 239, 332
FatI	CATG	2 cut(s)	364, 424
FokI	GGATG	1 cut(s)	423
FspBI	CTAG	1 cut(s)	275
HaeIII	GGCC	3 cut(s)	129, 340, 374
Hin1II	CATG	2 cut(s)	368, 428
HinfI	GANTC	2 cut(s)	22, 251
Hpy166II	GTNNAC	2 cut(s)	191, 422
Hpy188I	TCNGA	1 cut(s)	256
Hpy188III	TCNNGA	2 cut(s)	136, 179
Hpy8I	GTNNAC	2 cut(s)	191, 422
HpyCH4V	TGCA	4 cut(s)	162, 191, 283, 335
HpyF10VI	GCNNNNNNNGC	1 cut(s)	280
HpyF3I	CTNAG	4 cut(s)	95, 185, 253, 311
Hsp92II	CATG	2 cut(s)	368, 428
Kzo9I	GATC	1 cut(s)	138
LpnPI	CCDG	8 cut(s)	32, 40, 48, 82, 255, 327, 354, 393
MaeI	CTAG	1 cut(s)	275
MalI	GATC	1 cut(s)	140
MboI	GATC	1 cut(s)	138
MboII	GAAGA	2 cut(s)	279, 298
MfeI	CAATTG	1 cut(s)	358
MhlI	GDGCHC	1 cut(s)	193
MlsI	TGGCCA	1 cut(s)	374
MluCI	AATT	2 cut(s)	207, 358
MluNI	TGGCCA	1 cut(s)	374
MlyI	GAGTC	1 cut(s)	245
MmeI	TCCRAC	1 cut(s)	35
MnlI	CCTC	7 cut(s)	140, 143, 306, 309, 383, 391, 410
Mox20I	TGGCCA	1 cut(s)	374
MscI	TGGCCA	1 cut(s)	374
MseI	TTAA	1 cut(s)	113
MslI	CAYNNNNRTG	1 cut(s)	83
Msp20I	TGGCCA	1 cut(s)	374
MspR9I	CCNGG	1 cut(s)	342
MunI	CAATTG	1 cut(s)	358
MvaI	CCWGG	1 cut(s)	342
MwoI	GCNNNNNNNGC	1 cut(s)	280
NdeII	GATC	1 cut(s)	138
NheI	GCTAGC	1 cut(s)	274
NlaIII	CATG	2 cut(s)	368, 428
NlaIV	GGNNCC	1 cut(s)	327
PfeI	GAWTC	1 cut(s)	22
PflMI	CCANNNNNTGG	1 cut(s)	370
PleI	GAGTC	1 cut(s)	245
PpsI	GAGTC	1 cut(s)	245
PsiI	TTATAA	1 cut(s)	384
Psp6I	CCWGG	1 cut(s)	340
PspGI	CCWGG	1 cut(s)	340
PspN4I	GGNNCC	1 cut(s)	327
RsaI	GTAC	2 cut(s)	293, 421
RsaNI	GTAC	2 cut(s)	292, 420
RseI	CAYNNNNRTG	1 cut(s)	83
SaqAI	TTAA	1 cut(s)	113
Sau3AI	GATC	1 cut(s)	138
SchI	GAGTC	1 cut(s)	245
ScrFI	CCNGG	1 cut(s)	342
SduI	GDGCHC	1 cut(s)	193
SetI	ASST	5 cut(s)	186, 222, 302, 402, 421
SmiMI	CAYNNNNRTG	1 cut(s)	83
Sse9I	AATT	2 cut(s)	207, 358
SspMI	CTAG	1 cut(s)	275
StyD4I	CCNGG	1 cut(s)	340
TasI	AATT	2 cut(s)	207, 358
TatI	WGTACW	1 cut(s)	291
TfiI	GAWTC	1 cut(s)	22
Tru1I	TTAA	1 cut(s)	113
Tru9I	TTAA	1 cut(s)	113
TscAI	CASTG	1 cut(s)	315
TspDTI	ATGAA	2 cut(s)	220, 339
TspRI	CASTG	1 cut(s)	315
Van91I	CCANNNNNTGG	1 cut(s)	370
VneI	GTGCAC	1 cut(s)	189
XspI	CTAG	1 cut(s)	275

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.